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Citations for Becton, Dickinson and Company :
7801 - 7850 of 8130 citations for Protein Kinase A PKA Activity Assay Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... and CD43-B cells were cultured at a density of 5×105 cells per ml in RPMI medium supplemented with 15% FBS and 25 ng ml−1 of IL-4 (R&D) and anti-CD40 (BD Biosciences). Cultured cells were maintained daily at a density of 0.5-1×106 cells per ml ...
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bioRxiv - Cell Biology 2023Quote: ... NTHi was inoculated into 50 ml brain-heart-infusion broth (Roth, Germany) with 1% supplement B (BD Difco™; BD, Germany) and incubated overnight at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibody incubation was overnight at 4 degrees C with the following antibodies at 1:100 dilution in PBS + 3% BSA: CD24-FITC (clone ML5, BD Bioscience), CD271-APC (clone C40-1457 ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 x 106 dissociated organoid cells in 100 µl hepatocyte culture medium and 5% Matrigel were injected subcutaneously into the flank using a 1 ml syringe with 25G needle (BD 305122). For human grafts ...
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bioRxiv - Biochemistry 2023Quote: ... the cells were resuspended with 500 μl PBS containing 1% BSA for detection in BD Accuri C6 flow cytometer system (BD Biosciences) at excitation 488 nm and emission 519 nm ...
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bioRxiv - Immunology 2023Quote: ... Bacteria were subcultured from the chocolate agar into brain heart infusion (BHI) broth supplemented with 1% BBL IsoVitaleX (BD Biosciences, # 211875) to a starting OD600=0.1 ...
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bioRxiv - Cell Biology 2023Quote: ... PECAM was stained 1:200 with the live labeling antibody Alexa Fluor® 647 Mouse Anti-Human CD31 (BD Pharmingen™), 2 min prior to imaging ...
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bioRxiv - Immunology 2023Quote: ... single cell suspensions were incubated with 1 µg/ml SIINFEKL peptides in complete RPMI medium for 1 h at 37°C followed by brefeldin A (Golgiplug; BD Pharmingen) addition and further 5 h incubation ...
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bioRxiv - Immunology 2023Quote: 50 × 103 effector cells were cultured alone or together with 5 × 103 tumour cells for 4 h at a 10:1 ratio in the presence of anti-CD107a-PE mAb (clone: H4A3; BD Biosciences) and monensin (3 μM ...
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bioRxiv - Immunology 2023Quote: ... and CD4+FYP+ Treg cells at indicated Treg : Tresp ratios in the presence of 1 μg/ml anti-CD3 antibody (BD Pharmingen). After 3 days ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were incubated with 300 µg/mL DNase for 1 hour at 37°C to expose incorporated brdU and then stained with an APC anti-brdU antibody (1:50, BD Biosciences). 1X BD Perm/Wash in ddH2O was used as a staining and washing buffer ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were then resuspended in BD Perm/wash buffer with the following antibodies: BV650 IL4 (1:50, BD Bioscience, cat#564004), APC-Fire750 IFNγ (1:80 ...
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bioRxiv - Immunology 2022Quote: ... GFP expression in peripheral blood B and T cells was screened using anti-CD19-PE (BD, clone 1D3, dilution 1/100), anti-CD3e-PerCP-Cy5.5 (eBioscience ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed five times and developed with tetramethylbenzidine (1-Step Ultra TMB-ELISA Substrate Solution, Thermo Fisher Scientific, for the RBD ELISA, and BD OptEIA Substrate Solution ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were resuspended in staining buffer consisting of 50% PBS containing 1% bovine serum albumin (BSA) and 50% Brilliant Stain Buffer (BD Biosciences) and incubated for 20 minutes at 4°C in the following antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... 100 μl of this suspension (1×10e5) was transferred to a 5 ml culture tube and 5 μl of FITC Annexin V (BD Biosciences) added ...
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bioRxiv - Biophysics 2022Quote: ... Saccharomyces cerevisiae strain W303-GAL4 was grown overnight in YPDA medium (yeast extract 1 %, adenine sulfate 540 μM, peptone 2 % (BD Biosciences), glucose 2 % ...
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bioRxiv - Cancer Biology 2023Quote: Single cell suspensions were pre-incubated for 15 minutes in a staining solution (PBS+/+, 2% FBS) containing 1 μg/mL Fc block (BD Biosciences), followed by an incubation with the antibodies for 45 minutes (Table S1) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... then the resulting 350 μL volume was drawn twice into a 1 mL luer slip tip tuberculin syringe (BD Catalog # 309659) fitted with a 27G-½” needle ...
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bioRxiv - Developmental Biology 2023Quote: ... and then resuspended in 100 μL Annexin V binding buffer with either (i) FITC-Annexin V conjugated antibody (1:33 dilution in TBST) and BD propidium iodide staining solution (BD Pharmingen) or (ii ...
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bioRxiv - Immunology 2023Quote: ... Single cell suspensions of ex vivo organs were co-cultured with DCs in complete RMPI at a ratio of approximately 10 T cells to 1 DC in the presence of Golgi Plug (BD Bioscience). Co-cultures were incubated at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... HC-04 cells were washed and fixed with 1% PFA in PBS before quantifying dextran-positive cells by flow cytometry (LSR II instrument, BD Biosciences). For data analysis using FlowJo V.10.0.8 (Tree Star) ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then incubated for 45 min at 4°C with rocking with 1:250 of each of the following antibodies: Negative selection markers: BV421 Rat anti-mouse anti-CD31 (562939, BD Biosciences), and BV421 Rat anti-mouse CD45 (563890 ...
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bioRxiv - Cancer Biology 2023Quote: ... CD49fhighEpCAMlowLin- cells from 2-month-old C57BL/6 WT mice were sorted using FACS and resuspended in culture media (DMEM/F12+2% FBS+1% PSA+2% B27) supplemented with EGF (10 ng/ mL, BD Bioscience), Rspo1(250 ng/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... CD49fhighEpCAMlowLin- cells were collected from FACS and cultured in 200 μL culture media (DMEM/F12+2% FBS+1% PSA+2% B27) supplemented with 10 ng/ mL EGF (BD Bioscience), 250 ng/ mL Rspo1(R&D) ...
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bioRxiv - Cancer Biology 2023Quote: ... mice bone marrow mononuclear cells were first stained with lineage (Lin) cocktail containing biotinylated antibodies against Ly-6G/Ly-6C (Gr-1) Biotin RB6-8C5 (BD Bioscience) (1:100) ...
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bioRxiv - Immunology 2022Quote: ... Single-cell suspensions were incubated with Fc block (anti-mouse CD16/CD32, 1 μg for 1x106 cells, BD Biosciences, Cat# 553142) at 4°C for 30 min ...
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bioRxiv - Immunology 2022Quote: ... cells were fixed with 2% paraformaldehyde for 20 min and resuspended in 350 ul 1% BSA/PBS for phenotyping by FACSDivacytometer (BD Biosciences). To set up the compensation control ...
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bioRxiv - Physiology 2023Quote: ... cells were incubated at 4°C for 30 min with APC-conjugated rat anti-mouse CD31 (1:200, BD Pharmigen, 551262). DAPI (5 mg/ml ...
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bioRxiv - Neuroscience 2023Quote: ... aristae or the third segment of antennae were surgically removed by a needle (BD PrecisionGlide Hypodermic Needles 18G x 1” BD 305195) connected to a syringe.
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bioRxiv - Immunology 2023Quote: ... and 1:20 dilution mouse anti-human CD123 (IL-3)-PE (stock 200 ug/mL, BD Biosciences, Franklin Lakes, NJ, USA). A 1:1,000 dilution of anti-mouse IgG Alexa-647 (stock 1 mg/mL ...
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bioRxiv - Microbiology 2023Quote: ... The resulting tissue homogenates were each serially diluted from 10-1 to 10-7 and plated in triplicates onto Marine Agar 2216 (MA, BD Difco). Following one week of incubation at 23 °C ...
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bioRxiv - Bioengineering 2023Quote: ... The embryos were then stained with monoclonal antibody against PECAM1 (CD31, clone MEC 13.3, Cat# 553700, BD Pharmingen, 1:200 dilution) and monoclonal anti-β-tubulin III (TuJ1) ...
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bioRxiv - Cancer Biology 2023Quote: ... and either 1×106 VCaP or 5×105 22Rv1 cells suspended in 100 μl of PBS with 50% Matrigel (BD Biosciences) were implanted subcutaneously into the dorsal flank on both sides of the mice ...
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bioRxiv - Immunology 2023Quote: ... Single cells were then washed with 1% Bovine serum albumin (BSA) in PBS and blocked with anti-Fcγ receptor (BD Biosciences). Human peripheral blood mononuclear cells (PBMC ...
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bioRxiv - Cell Biology 2023Quote: ... E8.5 embryos were fixed with 4% paraformaldehyde (PFA) in PBS at 4°C for 3 h and incubated with FITC-conjugated anti-EEA1 (1:100, BD Bioscience) or Cy3-labeled anti-mouse IgG (1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... The cells followed by an incubation with the SM/Chol probe OlyA (3 µM) and primary anti-IFN-γR1 antibody (1:50) (BD Biosciences) together in 1% BSA-PBS at room temperature for 90 min ...
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bioRxiv - Cancer Biology 2023Quote: ... After washing the slides were blocked for 30 min in Superblock solution following the incubation with the primary antibodies mouse anti-BrdU/IdU (1:100, BD Bioscscience) and rat anti-BRDU/CIdU (1:200 ...
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bioRxiv - Cancer Biology 2023Quote: ... and total DNA (7-AAD 1:50 solution dilution) and analyzed by flow cytometer in a BD LSRFortessa cell analyzer (BD Biosciences) using 488 nm and 640 nm lasers ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed twice in PBS-FBS 1% and stained with 20 µL of anti Ki-67 (FITC Mouse Anti-Ki-67 Set, BD Biosciences) for 30 min at RT in the dark ...
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bioRxiv - Neuroscience 2023Quote: ... Stained cells were analyzed and 7AAD−Lin−Vcam−Sca1+ (Stem cell antigen 1; Ly6a) (FAPs) were isolated using FACS Aria III cell sorter (BD Biosciences) with 4-way-purity precision ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed two times with 150 µL PBS (centrifuged 5 min at 400 × g at 4 °C) and resuspended in 50 µL Fc-block (1:50, Purified Rat Anti-Mouse CD16/CD32, BD, #553142) diluted in FACS buffer (1x PBS/2% FBS/2mM ...
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bioRxiv - Immunology 2023Quote: ... Effector cells were incubated with K562 and Nalm6 variants for 4 hours at a ratio of 5:1 with addition of Brefeldin A (GolgiPlug, BD Biosciences). Next ...
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bioRxiv - Immunology 2023Quote: ... Cell purity was determined by flow cytometry (see Figure S6A) using antibodies specific against CD20-FITC (1:100 345792, BD Biosciences), CD14-PerCP (1:50 ...
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bioRxiv - Cancer Biology 2023Quote: ... CD8/CD4 T cells ratio and activation was assessed with BD Multitest CD3/CD8/CD45/CD4 (1:20; BD Biosciences, #342317) and APC-CD69 (1:100 ...
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bioRxiv - Microbiology 2024Quote: ... Cells were activated for 3 days with 1 μg/mL/million cells of purified NA/LE mouse anti-human CD3 and CD28 (BD Biosciences) and infected with HIVBaL at 0.01 ng/106 cells ...
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bioRxiv - Immunology 2024Quote: ... CFSE-labelled cells were boosted with HIV-1-Gag or vaccine-specific peptide pools in the presence of Brefeldin A solution (BD, Biosciences) and Monensin solution (BD Biosciences) ...
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bioRxiv - Cell Biology 2024Quote: ... ESLAM HSCs defined as CD45+EPCR+CD48-CD150+ as previously described,1 were isolated using a Becton Dickinson Influx sorter (BD Biosciences). Cells were sorted directly into culture dishes containing media or into Eppendorfs containing FACS buffer.
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bioRxiv - Molecular Biology 2024Quote: ... unsorted, sorted, and depleted fractions, were labelled with mouse anti-ASGPR1-PE (1:20 dilution, to detect porcine hepatocytes) (BD Pharmigen), rat anti-OC2-2F8 ...
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bioRxiv - Neuroscience 2024Quote: ... The organs of corti were then dissected from the otic capsule and co-stained with anti-Ctbp2 antibodies (1:800; 612044, BD Transduction) and anti-myosin VI antibodies (1:400 ...