Labshake search
Citations for Becton, Dickinson and Company :
7701 - 7750 of 7984 citations for Thiol Fluorescent Detection Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2024Quote: ... Additional samples were stained and analyzed as above using the following modifications: the samples were blocked with FcR block (1:200 dilution; 553141, BD Biosciences) for 10 min at 4°C and incubated with anti-mouse CD11b-PE (1:50 dilution ...
-
bioRxiv - Bioengineering 2024Quote: ... 3 ml of spiked blood mixed with BD BACTEC Plus Aerobic medium (BD, USA) (3:1 Blood:BD BACTEC Plus Aerobic medium) was gently placed over the density media ...
-
bioRxiv - Immunology 2024Quote: Activation of signaling pathways downstream of pre-TCR was analyzed by western blotting of cells incubated with 10 µg/ml of anti-CD3ε (UCHT-1; BD Biosciences) or anti-pTα (K5G3 ...
-
bioRxiv - Microbiology 2024Quote: ... and then stained with a panel of immunophenotyping antibodies (clone, dilution, manufacturer) at room temperature for 30min: CD45 BUV395 (30-F11, 1:400, BD Biosciences), CD24 BV510 (M1/69 ...
-
bioRxiv - Immunology 2024Quote: ... and each tube was stained with 5 μL of APC-Cy7 Mouse Anti-Human CD31 (PECAM-1) antibody (clone WM59, BD Biosciences) and 5 μL of APC anti-human CD144 (VE-Cadherin ...
-
bioRxiv - Cell Biology 2024Quote: ... Tissue and cell slides were then blocked with 1% BSA and 10% normal goat serum in PBS at room temperature for 1 hour followed by overnight incubation at 4°C with primary antibodies (1:50 dilution) against human HIF1α (cat # 610958, BD Biosciences), rat HIF1α (cat # NB100-105 ...
-
bioRxiv - Immunology 2024Quote: ... fluorescens J4 clinical isolates were grown for 1 day at 30°C in YM broth (BD Biosciences, San Diego, CA, USA). After gavages ...
-
bioRxiv - Molecular Biology 2024Quote: ... The emission wavelength ratios of intracellular Ca2+-bound and unbound Indo-1-AM were analyzed on a LSRII flow cytometer with FACSDiva software (BD Biosciences).
-
bioRxiv - Molecular Biology 2024Quote: ... were stimulated by incubation with 5 µg ml-1 anti-IgM F(ab’)₂ fragment antibody (115-006-020; Jackson Immuno Research) with or without 5 µg ml-1 anti-CD40 antibody (553787, BD Pharmingen), with 100 ng/ml MPL-A (Invivogen ...
-
bioRxiv - Molecular Biology 2024Quote: ... Antibody staining for Cxcr4 was performed for 20 mins on ice using 1:100 APC-conjugated Rat anti-Mouse CD184/Cxcr4 (BD Pharmingen) in FC buffer and cells were then washed twice in FC buffer and resuspended in 1 μg/ml DAPI in FC buffer at ∼1-1.5 x 1E7 cells/ml to label dead cells and run the suspension through a cell strainer ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells were then incubated with the primary antibody solution (1:100 dilution; PDGFRA-APC, Thermo Fisher, cat#17-1401-81; SCA1-FITC, Thermo Fisher, 11-5981-82; CD45-BV450, BD Biosciences ...
-
bioRxiv - Cell Biology 2020Quote: ... resuspended in buffer solution (PBS + 2% FBS + 2mM EDTA) and blocked with recombinant Fc protein (1:100 dilution, BD Biosciences, cat#553142). Cells were then incubated with the primary antibody solution (1:100 dilution ...
-
bioRxiv - Cell Biology 2020Quote: ... BSA was removed and the desired mouse primary antibody was added in Antibody solution (0.5%BSA/0.01% Triton X-100/PBS) at the indicated dilutions: Anti-β-Catenin Clone 14/Beta-Catenin (BD Biosciences, 1:1000), anti-Lamin A (Abcam ...
-
bioRxiv - Immunology 2022Quote: ... 10% FCS and incubated with the indicated pool of peptides (5 μg/ml final concentration of each pool) and brefeldin A (1 μg/ml; BD Pharmingen, USA) at 37°C for 12-16 hours ...
-
bioRxiv - Biochemistry 2022Quote: ... 10 μL pre-cleared cell lysate was combined with 200 μL assay reagent containing 1 mM D-luciferin (BD Biosciences, product #556878), 3 mM adenosine triphosphate ...
-
bioRxiv - Neuroscience 2021Quote: ... Primary antibodies used for immunofluorescence of HeLa cells and/or primary neurons were: mouse anti-Ataxin-2 (BD Biosciences 611378, 1:250), mouse anti-beta-III-tubulin (Covance MMS-435P ...
-
bioRxiv - Microbiology 2019Quote: ... The cell pellet was then resuspended in 1 ml of T-R minimal medium and injected into the IsoCaRB system using a 3 ml syringe (BD Biosciences # 309657) and a 20-gauge needle (Sigma-Aldrich #Z192511).
-
bioRxiv - Synthetic Biology 2019Quote: ... It was sterilized by autoclaving after filling with 2.5 L animal component free complex medium containing 20 g L-1 yeast extract (BD Biosciences, Heidelberg, Germany), 40 g L-1 peptone from soymeal (Applichem ...
-
bioRxiv - Cancer Biology 2020Quote: ... cells were washed with 1x Permeabilization buffer (eBioscience) and incubated for 15 min in FACS buffer (PBS, 2% FCS) before adding the primary MASH-1 antibody (BD Biosciences, 556604), 1:250 ...
-
bioRxiv - Cancer Biology 2020Quote: ... cells were washed with 1x Permeabilization buffer (eBioscience) and incubated for 15 min in FACS buffer before adding the primary MASH-1 antibody (BD Biosciences, 556604), 1:250 ...
-
bioRxiv - Cell Biology 2019Quote: ... Hematopoietic cells were harvested from OP9-DL1 cocultures by forceful pipetting and stained on ice for one hour with the following antibodies: biotin-CD4 (1:2000; BD Pharmingen, 553045), biotin CD8a (1:2000 ...
-
bioRxiv - Cancer Biology 2020Quote: ... Cells were then washed with PBS and resuspended in 0.5 ml of PBS containing 1 μg/ml propidium iodide (PI) and 50 μg RNase and analyzed by flow cytometry (BD LSR-II) MCF-7 cells were exposed to 10 Gy γ radiation ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... 4 μL of the saturated culture were transferred in 60 uL PBS and the samples were stored at 4 °C for 1-2 days before being assayed by flow cytometry (BD FACSCanto II). Data were analyzed in FlowJo ...
-
bioRxiv - Cell Biology 2020Quote: ... cells were pelleted and washed twice with 1% FBS in PBS and subjected to flow cytometry using a FACSMelody™ (BD Biosciences).
-
bioRxiv - Immunology 2021Quote: Mouse splenocytes were cultured in RPMI-10 and stimulated with the S peptide pool at 1 µg/ml/peptide in the presence of GolgiStop™ and GolgiPlug™ (BD) for 6 hours ...
-
bioRxiv - Immunology 2021Quote: For TCR stimulation naïve and primed CD8+ T cells were incubated for 20 min at 4°C with biotinylated anti-CD3ε mAb (5 and 1 μg/mL, BD Biosciences #553060). Cells were washed and stimulated for the indicated time by adding streptavidin (20 μg/mL ...
-
bioRxiv - Bioengineering 2020Quote: ... we also preincubated 200 µL of 2 µmol recombinant B56 protein with 1 µmol mouse anti-B56 antibody (Clone 23/B56α BD biosciences, 610615) and 1 µmol goat anti-mouse IgG Alexa Fluor 647 (Thermo Fisher A-21235 ...
-
bioRxiv - Bioengineering 2021Quote: ... and stained for 1 hour at 4 °C with 2 µl of the following Abs: PE-Cy7 conjugated anti-mouse IFN-γ (BD Biosciences), PE conjugated anti-mouse IL-2 (Invitrogen eBioscience) ...
-
bioRxiv - Cell Biology 2021Quote: ... was added after 1 hour and cells were stained for surface NK makers (CD56 and CD16) and intracellular IFN-γ (Cytofix/Cytoperm; BD Biosciences).
-
bioRxiv - Cancer Biology 2020Quote: ... 0.1% sodium azide) and re-suspended in 500µl of ice-cold FACS buffer prior to flow cytometric analysis (BD LSR II Fortessa). Data were analyzed using the FCS Express software.
-
bioRxiv - Cancer Biology 2020Quote: ... at a density of 1 million cells per 100 μL and stained with anti-mouse CD16/CD32 Fc Block™ (BD Pharmigen) for 10 min ...
-
bioRxiv - Microbiology 2020Quote: ... Sections were washed and stained with primary antibody to E-cadherin (1:100 anti E-cadherin mouse monoclonal antibody, BD Biosciences 610181) and/or primary antibody to O157-antigen (1:2000 anti E ...
-
bioRxiv - Microbiology 2021Quote: ... 2×106 lymph node cells were stained with the following monoclonal antibodies: rat anti-mouse CXCR5-biotin (clone 2G8, 1:100, BD, cat#551960), streptavidin PECy7 ...
-
bioRxiv - Cancer Biology 2020Quote: ... then probed with the following primary antibodies in 5% milk/TBS-T overnight at 4 °C: SREBP-2 (1:250; BD Biosciences, 557037), p44/42 MAPK (ERK1/2 ...
-
bioRxiv - Cell Biology 2022Quote: ... washed three times with RPMI and incubated for 30 mins at 37°C in RPMI containing Dispase (BD Cornig, 1:10 dilution) and 30 μg/μl DNase I with continuous and gentle agitation on a rotating shaker ...
-
bioRxiv - Neuroscience 2022Quote: ... The primary and secondary antibodies and dilutions used here were: mouse anti-BrdU 1:100 (347580; BD Biosciences, Franklin Lakes, NJ, USA), 4c4 1:200 (mouse anti-fish leukocytes ...
-
bioRxiv - Microbiology 2020Quote: The mitochondrial inner membrane potential (Δψm) was determined by staining with the membrane-permeable lipophilic cationic fluorochrome JC-1 (BD Biosciences, NJ). Overnight C ...
-
bioRxiv - Molecular Biology 2020Quote: ... DNA extraction and BrdU-labelled DNA immunoprecipitation were performed with anti-BrdU antibody (40 μL of 25 μg mL−1, BD Pharmingen, #555627). Validation of BrdU immunoprecipitation was carried out using qRT-PCR on known Early (BMP1 ...
-
bioRxiv - Neuroscience 2021Quote: ... Cell suspensions were stained with the following antibodies in FACS buffer (1% fatty acid-free BSA + 1mM EDTA in PBS) for 25 minutes on ice: Fixable Viability Stain BV510 (BD Biosciences, 564406), CD11b-BV421 (BioLegend 101251) ...
-
bioRxiv - Immunology 2020Quote: ... and serum-blocked in 2.5% goat serum/PBS containing 1:100 dilution of Fc block (CAT # 553142; BD Biosciences, San Jose CA). For B ...
-
bioRxiv - Microbiology 2020Quote: ... Each culture contained a 10 μL 4.0 McFarland (McF) bacterial cell suspension (WHO-F or WHO-X) in 12 mL gonococcal (GC) broth supplemented with 1% IsoVitaleX (BD BBL™) and vancomycin ...
-
bioRxiv - Neuroscience 2020Quote: ... Primary antibodies were diluted in blocking solution of 1% normal horse serum in PBS with 0.3% Triton X-100 and incubated overnight at 37°C as follows: mouse (IgG1) anti-CtBP2 (C-terminal Binding Protein) 1:200 (#612044, BD Transduction Labs) targeting pre-synaptic ribbons ...
-
bioRxiv - Cancer Biology 2021Quote: ... and stained for 1 hour at 4 °C with 2 μl of the following Abs: PE-Cy7 conjugated anti-mouse IFN-γ (BD Biosciences), PE conjugated anti-mouse IL-2 (Invitrogen eBioscience) ...
-
bioRxiv - Microbiology 2021Quote: ... the same protocol was followed except that of the use of biotinylated anti-Syrian hamster IgG1 /IgG2 antibodies in 1:10000 dilution (BD biosciences, USA) and Streptavidin-horseradish peroxidase 1:8000 (Thermo-scientific ...
-
Mutation in the matricellular gene fibulin-4 leads to endothelial dysfunction in resistance arteriesbioRxiv - Physiology 2022Quote: ... Membranes were blocked in 5% BSA/0.1% Tween20 in 1x TBS or 5% milk/0.1% Tween20 in 1x PBS for primary antibody mouse anti-human phospho-eNOS (1:1,000, pS1177, #612392, BD Biosciences, Franklin Lakes, NJ) or mouse anti-human-eNOS (1:1,000 ...
-
bioRxiv - Immunology 2022Quote: ... 1x105 tumor cells and 1x105 γδ T cells were co-cultured for 3 h at 37°C in the presence of 1 µl α-CD107a-PE antibody (BD Biosciences). Medium or stimulation with α-CD3 and α-CD28 (both 1 µg/ml ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were washed the pellet was resuspended with 1 ml PBS and analyzed in a Flow Cytometer LSRFortessa (BD Biosciences, NJ, USA). Analysis of the data output was preformed via FlowJo v10.8 software using Watson model to calculate the percent of cells in each stage of the cell cycle.
-
bioRxiv - Cell Biology 2022Quote: ... Hematopoietic cells were harvested from OP9-DL1 cocultures by forceful pipetting and stained on ice for one hour with the following antibodies: Pacific Blue CD4 (1:500; BD Pharmingen, 558107), BV711 CD8a (1:1000 ...
-
bioRxiv - Bioengineering 2022Quote: ... HAECs were allowed to recover from passaging for 24 h after plating and were subsequently incubated with AlexaFluor-647 anti-human VE-Cadherin antibody (1:100, BD Biosciences, #561567). After 15 minutes incubation ...
-
bioRxiv - Developmental Biology 2019Quote: ... Then the cells were washed twice in 1 ml PBS with 10% FBS and examined on a BD FACSAria™ III cell sorter (BD Biosciences).