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Citations for Becton, Dickinson and Company :
701 - 750 of 2473 citations for Recombinant Human MSLN Protein Fc Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Samples were treated with BD Fc Block (Rat Anti-mouse CD16/CD32; BD Biosciences, USA) for 20 min at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... liver lymphocytes were treated with an Fc block (anti-CD16/32, clone 2.4G2; BD Biosciences) and live/dead dye for 30 min ...
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bioRxiv - Immunology 2023Quote: ... Antibody stainings were preceded by adding Fc block (anti-CD16/CD32; clone 2.4G2, BD Biosciences). For sorting ...
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bioRxiv - Immunology 2024Quote: Flow cytometry was performed using anti-mouse CD16/32 (Fc Block, clone 2.4G2, BD Biosciences) before staining with primary antibody or isotype controls ...
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bioRxiv - Neuroscience 2024Quote: ... The cells were first blocked for 10 minutes with Fc Blocker (cat#: BDB564765; BD Biosciences) and subsequently stained with antibodies (Supplementary Table 1 ...
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bioRxiv - Immunology 2024Quote: ... 1 x 106 cells were blocked using anti-CD16/CD32 antibodies (Fc block; BD Pharmingen) and then labeled with a panel of fluorochrome-conjugated antibodies including CD45 (30-F11 ...
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bioRxiv - Immunology 2021Quote: ... IL-1β was detected using biotinylated anti IL-1β (eBioscience) and streptavidin conjugated to horseradish peroxidase (BD Biosciences). Peroxidase activity was detected using an o-phenylenediamine hydrochloride (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2021Quote: ... The Tbr1 signals were amplified using biotinylated secondary IgG antibody (swine anti-rabbit IgG) (1:400, BD Biosciences) followed by Alexa Fluor 488 or 568 Streptavidin (1:100 ...
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bioRxiv - Developmental Biology 2020Quote: ... The Tbr1 signals were amplified using biotinylated secondary IgG antibody (swine anti-rabbit IgG) (1:400, BD Biosciences) followed by Alexa Fluor 488 or 568 Streptavidin (1:100 ...
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bioRxiv - Immunology 2023Quote: ... IFNγ was subsequently detected using a 0.1 μg/mL biotinylated rat anti-murine Ab (clone XMG1.2; BD Pharmingen) and recombinant IFNγ (BD Pharmingen ...
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bioRxiv - Biochemistry 2023Quote: ... followed by signal amplification using biotinylated rat anti-mouse IgG (Jackson Immunochemicals) and streptavidin-allophycocyanin (APC) (BD Biosciences). Propidium Iodide (Sigma-Aldrich p-4864 ...
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bioRxiv - Microbiology 2021Quote: ... creating either N- or C-terminally tagged GAL4 activation domain (AD/prey) or GAL4 DNA-binding domain (BD/bait) fusion proteins ...
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bioRxiv - Immunology 2023Quote: ... up to 2 x106 enriched or unenriched cells were labeled with an oligonucleotide-tagged antibody sample tag (BD Biosciences). From infant thymus and PBMC donors up to 5 populations were sorted after doublet ...
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bioRxiv - Immunology 2021Quote: ... or for 4 h with 20 ng/ml recombinant mouse IL-4 (BD Pharmigen). Cells were then washed twice with isotonic buffer composed of 147 mM NaCl ...
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bioRxiv - Microbiology 2019Quote: Human erythrocytes were harvested from 10 mL of human blood following treatment in sodium heparin tubes (BD). Whole blood was centrifuged at 500 × g for 10 min at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... Human IFN-γ ELISA Set (555142) and Human IL-2 ELISA Set (555190) were purchased from BD OptEIA™ ...
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bioRxiv - Immunology 2020Quote: ... CXCR5 was stained with biotinylated anti-CXCR5 (2G8) followed by staining with streptavidin-conjugated PE (both from BD Biosciences). Cell viability was assessed using the Fixable Dye Ghost 510 (Tonbo Bioscience ...
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bioRxiv - Molecular Biology 2021Quote: ... biotinylated antibody was detected with Streptavidin-HRP (1:1’000 in 1% skim milk in 0.1% PBS-T, BD Biosciences). Chromogenic reaction and reading of plates were performed as described in (24) ...
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bioRxiv - Immunology 2022Quote: ... and biotinylated RBD Gamma and Delta with streptavidin-BV480 or streptavidin-BV650 (all from BD Biosciences, San Jose, CA) at a protein ...
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bioRxiv - Immunology 2023Quote: ... Twenty 8-11 months-old Gata1low mice were randomly divided in two groups which were treated either with vehicle (2% v/v dimethyl sulfoxide in H2O) or with biotinylated mAb RB40.34 (BD Pharmigen ...
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bioRxiv - Immunology 2023Quote: ... plates were washed three times again followed by incubation with biotinylated anti-IL-6 (1:500, BD Biosciences, #554402) and anti-TNF antibodies (1:250 ...
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bioRxiv - Immunology 2023Quote: ... plates were washed three times again followed by incubation with biotinylated anti-IL-6 (1:500, BD Biosciences, #554402) and anti-TNF (1:250 ...
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bioRxiv - Immunology 2023Quote: ... 2) As the MojoSort antibodies are biotinylated we replaced the Qa2-bio antibody with Qa2-BV421 (BD, Cat. 743309). Using qPCR and FACS we ensured that the positively selected (lineage positive ...
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bioRxiv - Immunology 2019Quote: Fc receptors were blocked with appropriate sera or rat-anti-mouse CD16/CD32 (2.4G2; BD Biosciences). All labelings were performed in PBS containing 2% heat-inactivated fetal calf serum (FCS ...
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bioRxiv - Microbiology 2020Quote: ... Samples were then blocked with CD16/CD32 (1:500; Fc Block™; BD Biosciences, RRID: AB_394656) for 5 min and incubated for 30 min with optimal concentrations of fluorochrome-conjugated antibodies (Table S2 ...
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bioRxiv - Bioengineering 2019Quote: ... cells were incubated with Fc block solution (purified anti-mouse CD16/CD32, clone 2.4G2, BD Biosciences) for 15 min at room temperature to prevent non-specific binding ...
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bioRxiv - Cancer Biology 2020Quote: ... RBCs were lysed and samples were subsequently incubated with anti-mouse Fc block (BD Pharmingen, 553142) for 10 minutes at 4°C ...
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bioRxiv - Cancer Biology 2019Quote: Single cell suspensions were incubated on ice with mouse Fc receptor block (BD Biosciences, 1:200) followed by antigen-specific antibodies in FACS buffer ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then incubated with Fc block (1:500 dilution, Cat. # 553142, clone 2.4G2, BD Biosciences) diluted in FACS buffer for 5 minutes to block nonspecific binding of antibodies to cellular Fc receptors ...
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bioRxiv - Immunology 2021Quote: ... for 10 minutes followed by an Fc block (catalog number 553142, BD Pharmingen, Franklin Lakes, NJ) at 1:1,000 for 15 minutes at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Flow Cytometry Standard (FCS) 3.0 files were analyzed with FlowJo software version X 0.7 (BD Biosciences).
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bioRxiv - Immunology 2020Quote: ... Barcoded cells were then combined into a single tube prior to Fc receptor blocking (BD Biosciences) and staining with a cocktail of metal-conjugated antibodies against cell-surface markers for 30 minutes at RT with frequent gentle shaking ...
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bioRxiv - Immunology 2020Quote: Single cell suspensions were first stained with anti-mouse CD16/CD32 (Fc-block; BD Biosciences; 553142) and dead cells were excluded with the Fixable Viability Dye eFluor506 (eBioscience ...
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bioRxiv - Immunology 2020Quote: ... Fc receptors were blocked using α-CD16/CD32 antibody (clone 2.4G2, BD Biosciences, Bedford, MA, USA). The cells were then stained with directly labeled antibody against CD5 (APC ...
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bioRxiv - Immunology 2022Quote: Splenocyte cell suspensions (~2 million cells in HBSS) were incubated with Fc block (553142, BD Biosciences) for 10 min on ice followed by the direct addition of the following antibodies ...
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bioRxiv - Cancer Biology 2022Quote: ... Final gating analysis was conducted using FCS Express software (De Novo) or FlowJo V10.8.1 (BD Biosciences). Control wells containing no chemokine in the bottom well were averaged and normalized as 100% migration ...
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bioRxiv - Immunology 2019Quote: ... Non-specific binding was blocked with rat anti-mouse CD16/CD32 Fc block (2.4G2, BD Biosciences) for 20 minutes at 4°C ...
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bioRxiv - Immunology 2020Quote: ... Single cell suspensions of BAL and lung digests were preincubated with Fc Block antibody (BD PharMingen) in PBS + 2% heat-inactivated FBS for 10 min at room temperature before staining ...
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bioRxiv - Immunology 2021Quote: ... supplemented with 10% FCS at 37 °C with either PMA/ionomycin (Leukocyte activation cocktail, BD Biosciences) for 3 hours ...
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bioRxiv - Cancer Biology 2019Quote: ... Fc receptors were blocked with anti-FcR (anti-CD16 CD-32 at 5μg/ml, BD Pharmingen). After 2 washes in PBS 2% FCS ...
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bioRxiv - Immunology 2021Quote: ... and with Mouse BD Fc Block™ (≤ 1 μg/million cells in 100 μl, BD Biosciences) at 4°C for 10 min ...
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bioRxiv - Immunology 2021Quote: ... while in PBS and then resuspended in rat anti-mouse CD16/32 (Fc block, BD Biosciences) antibody for 10 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... Myelin-depleted cell suspensions were incubated with an anti-CD16/CD32 Fc-blocking antibody (BD Biosciences) for 30 minutes at 4 °C to avoid Fc receptor binding and then washed once with FACS buffer ...
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bioRxiv - Immunology 2021Quote: ... cells were blocked in FACS buffer supplemented with anti-CD16/CD32 antibody (Fc Block, BD Pharmingen).
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bioRxiv - Immunology 2020Quote: Single cell suspensions were first stained with anti-mouse CD16/CD32 (Fc-block; BD Biosciences; 553142) and dead cells were excluded with the Fixable Viability Dye eFluor506 (eBioscience ...
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bioRxiv - Immunology 2021Quote: ... and cells were blocked in FACS buffer containing Fc Block anti-CD16/32 (2.4G2) (BD Biosciences), 5% normal hamster serum ...
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bioRxiv - Immunology 2021Quote: ... Unspecific binding by the Fc receptor was prevented by incubation of anti-mouse CD16+CD32 (BD) for 20 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... Single cell suspensions were incubated on ice with mouse Fc receptor block (BD Biosciences, 1:200) followed by antigen-specific antibodies in FACS buffer ...
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bioRxiv - Immunology 2022Quote: ... resuspended in Fc Block (clone 2.4G2, Becton, Dickinson, and Company [BD, Franklin Lakes, NJ], cat. 553142) and incubated for 5 minutes at 4°C ...
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bioRxiv - Microbiology 2024Quote: MLN cell suspensions were incubated with Fc-block (anti-CD16/CD32, BD Biosciences, cat no. 553142) followed by staining with antibodies against surface and intranuclear markers ...