Labshake search
Citations for Becton, Dickinson and Company :
701 - 750 of 903 citations for C terminal Proghrelin Isoform Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: Cell-free supernatants were stored at –20°C until their analysis for chemokine/cytokine content with a multiplexed bead-based immunoassay (BD(tm) Cytometric Bead Array) ...
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bioRxiv - Immunology 2021Quote: ... Cells were centrifuged and re-suspended in PBS and stored at 4°C prior to cytometric analysis using a using LSRFortessa flow analyzer (BD Biosciences). Results were analyzed using FlowJo V10 software (Flowjo ...
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bioRxiv - Immunology 2022Quote: ... The tube was then stored in the dark at 4° C for up to 2 hours prior to acquisition on the LSRII or FACSLyric analyzers (BD Biosciences).
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bioRxiv - Cancer Biology 2022Quote: ... Cells were incubated for 15 min at 25 °C in the dark and then analyzed using flow cytometry with a BD FACSCanto II device (BD Biosciences). All data are shown as mean ± standard deviation and are representative of three independent experiments ...
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bioRxiv - Immunology 2022Quote: Splenocytes from infected mice were incubated for 5 hours at 37°C 5%CO2 in cRPMI prior intra-cytoplasmic detection of active-caspase 3 (BD Bioscience) using BD Fixation/Permeabilization kit (BD Bioscience) ...
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bioRxiv - Immunology 2022Quote: ... were re-stimulated in vitro for 5h at 37°C 5% CO2 in cRPMI supplemented with GolgiStop (1/250; BD bioscience), GolgiPlug (1/500 ...
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bioRxiv - Immunology 2022Quote: ... stained on ice for 30’ with relevant cocktails of antibodies and populations of interest were sorted at 4°C on an BD FACSARIA (BD Bioscience) using a 70 μM nozzle in 50% FBS RPMI ...
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bioRxiv - Immunology 2022Quote: ... intracellular cytokine staining was performed at 4°C for 30 minutes using a cocktail of antibodies resuspended in 1x Perm/Wash buffer (BD Biosciences); intracellular antibodies included IFNγ APC-Cy7 (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... cultured splenocytes were resuspended in 40 μL FACS buffer containing fluorochrome-conjugated antibodies and incubated 1 hr at 4 °C followed by cell fixation using Cytofix/Cytoperm buffer (BD Bioscience) for 20 min at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... at 37 °C for 16-18 h followed by the addition of Brefeldin A (5 μg/ml BD Biosciences #BD 555029) for the last 5 h of the incubation ...
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bioRxiv - Immunology 2022Quote: ... at 37 °C for 16-18 h followed by the addition of Brefeldin A (5 μg/ml BD Biosciences #BD 555029) for the last 5 h of the incubation ...
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bioRxiv - Neuroscience 2023Quote: ... and cells were washed and labeled with the following antibodies for 30 min at 4 °C: rat anti-CD8 APC (1:100, catalog no. 553035; BD Biosciences); rat anti-CD45 PE (1:100 ...
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bioRxiv - Immunology 2022Quote: ... 20 μm paraformaldehyde fixed lung sections from Mtb-mCherry infected I-Tomcat Ai6 and Sp140−/− I-Tomcat Ai6 mice were stained at 4°C overnight with BV421-labeled SIRP□ (P84, BD Biosciences), Pacific Blue–labeled B220 (RA3-6B2 ...
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bioRxiv - Immunology 2022Quote: ... Cells were then resuspended in FACS buffer (1% BSA in D-PBS) and kept at 4°C in dark until analyzed with a customized BD LSR Fortessa flow cytometer (BD Biosciences). Compensation was performed using ArC and UltraComp compensation beads (Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2022Quote: ... thermoglucosidasius DSM2542T was routinely grown on 2-SPY medium106 at 60 °C with 150 RPM shaking or on Tryptic Soy Agar plates (BD Biosciences) at 60 °C ...
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bioRxiv - Immunology 2022Quote: ... pErk1/2T202/Y204 and pAktS473 of activated CD4+ T cells was performed by fixing for 15 minutes at 37 °C with BD Cytofix (BD Biosciences), permeabilization for 30min on ice with BD Phosflow™ Perm Buffer III (BD Biosciences ...
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bioRxiv - Microbiology 2022Quote: ... of anaerobic incubation at 37°C in a GasPak jar with an EZ Anaerobe Container System Sachets with Indicator (BD BBL), multiple colonies from each plate were inoculated into two (three ...
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Podoplanin expression in fibroblasts determines lymph node architecture and adaptive immune functionbioRxiv - Immunology 2022Quote: Lymph node single cell suspension of 2.5×106 cells was incubated for 20 min at 4°C with a purified rat IgG2b anti-mouse CD16/CD32 receptor antibody (BD biosciences). Cells were stained with fluorochrome-conjugated antibodies against CD9 ...
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bioRxiv - Pathology 2023Quote: ... then incubated for 20 minutes at 4°C with a combination of the following mouse anti-human antibodies: AF488 anti-CD3 (clone UCHT1, BD Biosciences), PE anti-CD203c (clone NP4D6 ...
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bioRxiv - Cancer Biology 2023Quote: ... admixed with an equal number of HDFs expressing either S301D mutant or wildtype lamin A/C were re-suspended in 70 µl of Matrigel solution (BD Bioscience) and injected intradermally into the left and right side of the mouse back ...
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bioRxiv - Microbiology 2023Quote: ... Mtb strain H37Rv with plasmid pSMT3[Phsp60/DsRed] [9] was cultured at 37°C in complete Difco Middlebrook 7H9 Broth (BD Biosciences), supplemented with 10% ADC (BD Biosciences) ...
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bioRxiv - Developmental Biology 2023Quote: Hematopoietic cells were stained with the antibodies listed in Supp Table S1 for 20-30 min at 4°C and analyzed on a custom BD LSR Fortessa (BD Biosciences). For sorting ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibody incubation was overnight at 4 degrees C with the following antibodies at 1:100 dilution in PBS + 3% BSA: CD24-FITC (clone ML5, BD Bioscience), CD271-APC (clone C40-1457 ...
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bioRxiv - Microbiology 2023Quote: ... and Sharpe (MRS) plates supplemented with 1.5% agar at 37°C in anaerobic conditions (BD GasPak™ EZ Anaerobe Container System) overnight ...
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bioRxiv - Neuroscience 2023Quote: ... cells were incubated for 15 min at 4 °C with a purified mouse anti-rat CD32 FcγII (clone D34-485, Rat BD Fc Block) to block unspecific binding ...
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bioRxiv - Immunology 2023Quote: T cells were staining 30 min at 4°C with anti-human CD3-PE (Cyto Matin Gene, CMGCD3-P100) anti-CD3-PerCP (BD, 347344), anti-CD3-FITC (BD ...
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bioRxiv - Immunology 2023Quote: ... The cells were cultured at 37°C and 5% CO2 for 72 hours and then the dye dilution was analyzed using a FACSVerse flow cytometer (BD Biosciences), and data were analyzed using FlowJo software (Tree Star).
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bioRxiv - Genomics 2023Quote: ... for 5 minutes and stained at a dilution of 1:50 with one of two panels of directly conjugated antibodies for 30 minutes at 4 °C: anti-human CD45 (BD 563792), CD204 (Biolegend 371906) ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were incubated with 300 µg/mL DNase for 1 hour at 37°C to expose incorporated brdU and then stained with an APC anti-brdU antibody (1:50, BD Biosciences). 1X BD Perm/Wash in ddH2O was used as a staining and washing buffer ...
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bioRxiv - Bioengineering 2023Quote: ... was grown overnight (37°C, 4% H2, 5% CO2, 91% N2, <10 ppm O2) in Chopped Meat Carbohydrate Broth (BD297307, BD, USA). Genomic DNA was extracted from B ...
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bioRxiv - Developmental Biology 2023Quote: ... for 30 minutes at 37°C and the single cell suspensions were filtered through a 70 µm cell strainer (BD Biosciences).
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bioRxiv - Cancer Biology 2022Quote: ... Cells were incubated for 30 minutes at 37°C washed and resuspended in ALDEFLUOR™ assay buffer and rapidly sorted on a FACSAria III cell sorting system (BD). 5×104 freshly sorted ALDHhi cells were seeded in seahorse XF24 plate in growth media and incubated overnight before seahorse analysis as described in analysis of extracellular flux section.
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then fixed (eBioscience #00-8222-49) at 4 °C for 20 minutes and permeabilization (eBioscience #00-8333-56) before staining for intracellular IFNγ (BV650, BD #563854) and TNFα (BV421 ...
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bioRxiv - Microbiology 2023Quote: Stained cells were stored for up to 2 days at 4°C in the dark (TRAIL-R1) and flow cytometry was performed on a BD FACSCanto II (BD Biosciences). Raw data were exported to FlowJo v10.8.1 for analysis and plotting.
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bioRxiv - Microbiology 2023Quote: ... PBMCs were then fixed in 2% formaldehyde and stored at 4°C until flow cytometry on an LSR II flow cytometer (BD Biosciences). Cell surface markers included CD3 (clone 17A2 ...
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bioRxiv - Physiology 2023Quote: ... cells were incubated at 4°C for 30 min with APC-conjugated rat anti-mouse CD31 (1:200, BD Pharmigen, 551262). DAPI (5 mg/ml ...
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bioRxiv - Immunology 2024Quote: ... surface antibodies were incubated with cells for 30 minutes at 4°C in hFACS buffer and Brilliant Stain Buffer (BD Biosciences). For intracellular staining ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The activated Ramos cells were incubated overnight (18 h) in 5% CO2 at 37°C followed by staining for flow cytometry (BD LSR2) and analysis (FlowJo and Graphpad Prism).
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bioRxiv - Microbiology 2023Quote: Bacteria used in this work are listed in Supplementary Table 7 and were grown at 37 °C in brain and heart infusion (BHI) broth (with agitation) and agar media (BD Difco). Cell lines of human origin used in this work included JEG-3 trophoblasts (ATCC HTB-36) ...
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bioRxiv - Immunology 2023Quote: ... The tube was then stored in the dark at 4°C for up to 2 hours prior to acquisition on a LSRII or FACSLyric analyzer (BD Biosciences).
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bioRxiv - Immunology 2023Quote: ... the cells were stained with the corresponding FACS panels (Extended Data Table 5) at 4 °C for 30 minutes and sorted using BD Aria III (BD Biosciences) or MoFlo Astrios EQ (Beckman Coulter ...
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bioRxiv - Neuroscience 2023Quote: ... The samples were incubated overnight at 4°C in the dark and sample analysis performed the following day using an LSR FortessaTM X-20 device (BD Biosciences).
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bioRxiv - Bioengineering 2023Quote: ... The cell suspension was incubated with the following antibodies for 30 minutes at 4 °C: CD106-PE (VCAM-1, 555647, BD Biosciences), CD62E-FITC (E-Selectin ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell suspensions were blocked for 5 min at 4 °C with rat anti-mouse CD16/CD32 (#553142, Mouse BD Fc Block, BD Biosciences) in FACS buffer ...
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bioRxiv - Cell Biology 2023Quote: ... E8.5 embryos were fixed with 4% paraformaldehyde (PFA) in PBS at 4°C for 3 h and incubated with FITC-conjugated anti-EEA1 (1:100, BD Bioscience) or Cy3-labeled anti-mouse IgG (1:500 ...
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bioRxiv - Cancer Biology 2023Quote: ... the pLCOs in suspension were centrifuged at 500 × g for 5 minutes at 4 °C and then resuspended in cold growth factor-reduced Matrigel (BD Biosciences). Subsequently ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed two times with 150 µL PBS (centrifuged 5 min at 400 × g at 4 °C) and resuspended in 50 µL Fc-block (1:50, Purified Rat Anti-Mouse CD16/CD32, BD, #553142) diluted in FACS buffer (1x PBS/2% FBS/2mM ...
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bioRxiv - Microbiology 2023Quote: ... fragilis NCTC 9343 ΔPSA44 (lacks PSA biosynthesis locus) maintained in 25% glycerol at −80 °C were thawed on Brain Heart Infusion agar plates (BHI, BD BBLTM) supplemented with 5 µg/ml hemin (Alfa Aesar ...
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bioRxiv - Cancer Biology 2023Quote: ... per well of U-bottom plate and coincubated for 4h at 37°C in the presence of NH4Cl and 1 µl Golgi PLUG (BD Biosciences). After that time cells were washed three times with 200 µl PBS and centrifuged (5 min ...
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bioRxiv - Bioengineering 2024Quote: ... Cells incubated with IL-10 at 37 °C were fixed and permeabilized for 20 min on ice with Cytofix/Cytoperm (BD Biosciences). Cells were washed twice in permeabilization buffer and further stained with anti-mouse IL-10 in permeabilization buffer for 30 min at on ice ...