Labshake search
Citations for Becton, Dickinson and Company :
7401 - 7450 of 7984 citations for Thiol Fluorescent Detection Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: ... and the cuticle between the eyes was cut with a needle (BD PrecisionGlide Hypodermic Needles 18G x 1” BD 305195) and removed with forceps ...
-
bioRxiv - Molecular Biology 2023Quote: The specimens were washed in PBS three times and incubated in 1:2000 v/v DAPI (BD Bioscience, NJ, USA) in PBS solution for 5 min.
-
bioRxiv - Neuroscience 2023Quote: ... All dissociated cells were blocked with Fc receptors staining buffer (1% anti-mouse CD16/CD32, 2.4 G2, 2% FBS, 5% NRS, and 2% NMS in HBSS; BD Bioscience) and then stained with a standard panel of antibodies ...
-
bioRxiv - Immunology 2023Quote: Both SS1 and A61C (1) (clinical strain) were revived from glycerol stock using brain heart infusion agar (BD Difco, USA) supplemented with 7% horse blood ...
-
bioRxiv - Immunology 2023Quote: ... Surface antigens were stained for 30 min at 4°C with indicated antibodies (Table.1) after Fc blocking for 4°C 30 min with anti-mouse CD16/32 (BD) without wash ...
-
bioRxiv - Immunology 2023Quote: ... and TG2-binding and non-TG2-binding IgA plasma cells were bulk-sorted into empty microtubes that had been coated with 1% BSA/PBS using a FACSAriaIII instrument (BD). For each of four untreated CeD patients ...
-
bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies were diluted in 3% BSA/PBST (0.1% Triton X-100 in PBS) and incubated with the samples at 37°C for 1 h: mouse anti-BrdU (BD Biosciences 347580 ...
-
bioRxiv - Microbiology 2023Quote: ... We then create a 1 mm thick sheet of agarose using a 3 mL syringe (BD, 3 mL Luer lock) and a blunt needle (Industrial Dispensing Supplies ...
-
bioRxiv - Cancer Biology 2023Quote: ... Single cells from digested xenograft tumors were labeled using a BUV395 anti-mouse cocktail (pooled and diluted 1:40: anti-mouse H2kD [BD Biosciences ...
-
bioRxiv - Cancer Biology 2023Quote: ... Single cells from digested xenograft tumors were labeled using a BUV395 anti-mouse cocktail (pooled and diluted 1:40: anti-mouse H2kD [BD Biosciences, cat. 742437], anti-mouse CD45 [BD Biosciences ...
-
bioRxiv - Cancer Biology 2023Quote: ... Single cells from digested xenograft tumors were labeled using a BUV395 anti-mouse cocktail (pooled and diluted 1:40: anti-mouse H2kD [BD Biosciences, cat. 742437], anti-mouse CD45 [BD Biosciences, cat. 567451], anti-mouse CD31 [BD Biosciences ...
-
bioRxiv - Cell Biology 2023Quote: We used the following antibodies for immunohistochemistry (IHC) and immunoblotting (IB): Rat anti-Mouse CD31 (BD 550274; IHC 1:200), GAPDH (Cell Signaling 5174S ...
-
bioRxiv - Bioengineering 2023Quote: ... Labelled cells were then washed in PBS + 1% BSA and analyzed on a FACS Canto II flow cytometer (BD Biosciences). Data analysis was performed using FlowJo software (Tree Star) ...
-
bioRxiv - Molecular Biology 2023Quote: ... two-color analysis of murine or human platelet activation was performed using fluorophore-labeled antibodies for P-selectin expression (Emfret Analytics, Wug.E9-FITC, #M130-1, and BD Biosciences ...
-
bioRxiv - Immunology 2023Quote: ... Single cell suspensions were incubated for 15 minutes at 4°C with anti CD16/CD32 Mouse BD Fc Block diluted 1/50 (BD) in 100 µL of FACS buffer in 96 wells round-bottom plates (Thermo Fisher Scientific) ...
-
bioRxiv - Immunology 2024Quote: ... Purity > 97% of CD14+ monocytes after magnetic sorting was verified by flow cytometry (anti-CD14-FITC clone MφP9, 1:40, #345784; BD Biosciences). For macrophage differentiation ...
-
bioRxiv - Immunology 2024Quote: ... The cells were resuspended in 1% bovine serum albumin (BSA) containing PBS and analyzed with a FACSCanto II (BD Biosciences) or an LSR Fortessa (BD Biosciences ...
-
bioRxiv - Developmental Biology 2024Quote: ... Both yolk sac tissues and whole embryos were incubated in primary antibody to rat anti-PECAM-1(BD Pharmingen, 553370) diluted in CAS-Block overnight at 4°C ...
-
bioRxiv - Neuroscience 2024Quote: The primary antibodies and corresponding dilutions used are as follows: (1) anti-CtBP2 mouse monoclonal antibody IgG1 (BD Transduction Laboratories) at 1:200 ...
-
bioRxiv - Immunology 2024Quote: ... Cells were washed twice with PBS and incubated for 20 minutes on ice with 1:100 dilution anti-CD16/32 (clone 2.4G2, BD biosciences) and 1:500 dilution Live/Dead nearIR dye (ThermoFisher) ...
-
bioRxiv - Molecular Biology 2024Quote: ... followed by stimulation for the appropriate time at 37°C with pre-warmed anti-hamster IgG1 antibody (5µg ml-1; G94-56, BD Pharmingen). Ex vivo expansion of B cells was performed using recombinant murine IL-4 (100 u ml-1 ...
-
bioRxiv - Biochemistry 2024Quote: ... The 1 mM Rhobo6 solution was then injected retro-orbitally using a 0.5 mL tuberculin syringe with 27G needle (BD, 305620). Mice were allowed to recover in their cage over 30 mins then euthanized by cervical dislocation ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells were pelleted and resuspended in PBS with DAPI (1:3000) and analysed with a BD FACS Canto (BD Biosciences) analyser ...
-
bioRxiv - Cell Biology 2024Quote: ... monoclonal mouse anti-human CD61 BV605 conjugated antibody and monoclonal anti-human PAC-1 FITC antibody were obtained from BD Bioscience (Franklin Lakes ...
-
bioRxiv - Molecular Biology 2024Quote: ... Antibodies for immunostaining were diluted in PBS with 3% BSA and 0.1% Tween-20 as follows: 1:800 rat anti-mouse CD31 (BD, 553370), 1:1000 rabbit anti-ERG (Abcam ...
-
bioRxiv - Immunology 2024Quote: ... diluted with fluorescence-activated cell sorting (FACS) buffer (PBS containing 0.1% sodium azide and 1% bovine serum albumin) and Brilliant Stain Buffer (BD Biosciences). The cells were washed in FACS buffer and fixed in PBS containing 4% paraformaldehyde (Electron Microscopy Sciences) ...
-
bioRxiv - Immunology 2024Quote: ... MD4 and Ars/A1-derived antibody secreting cells were detected with Biotin-conjugated Mouse Anti-Mouse IgM[a] (Clone:DS-1; BD Biosciences), followed by Streptavidin-AP (SouthernBiotech) ...
-
bioRxiv - Cancer Biology 2021Quote: ... Cells were resuspended in 500 m 2% FBS in HBSS and incubated on ice in the dark with 1 l Fc block (BD Biosciences) for 3 minutes ...
-
bioRxiv - Cancer Biology 2021Quote: ... PDX derived cells were centrifuged and suspended in ALDEFLUOR assay buffer containing Fixable Viability Stain 660 (FVS660, 1:1000, BD Horizon) after incubation at 37°C ...
-
bioRxiv - Systems Biology 2020Quote: ... White cells were resuspended in PBS supplemented with 1% human serum and 2 mM EDTA and analyzed on an LSR Fortessa instrument (BD Biosciences).
-
bioRxiv - Immunology 2021Quote: ... 1×105 Tconv cells were co-cultured with Treg cells at different ratios in the presence of 2×105 irradiated APCs and 1 µg/ml soluble anti-CD3 antibody (554829, BD Biosciences). The cells were cultured in T-cell complete media which consists of RPMI 1640 medium supplemented with 50 U/ml penicillin ...
-
bioRxiv - Immunology 2021Quote: ... for 5 minutes prior to staining at 4°C in the dark using the antibodies listed in supplementary table 1 and analysed using an LSR Fortessa cytometer (BD Biosciences) and FlowJo software (BD) ...
-
bioRxiv - Immunology 2021Quote: ... Slides were incubated overnight in blocking solution with the following primary antibodies (1:100): rat anti-mouse SiglecF (E50-2440, 552126 BD Biosciences), Armenian hamster anti-mouse CD80 (16-10A1 ...
-
bioRxiv - Immunology 2021Quote: ... Cells were then incubated with pS6 or HK2 antibody for 1 h and O-GlcNAc antibody for 20 min at RT in the dark in cytoplasmic permeabilization buffer (BD Biosciences). Nuclear permeabilization buffer was used for c-Myc staining ...
-
bioRxiv - Immunology 2021Quote: ... and surface stained for 30 mins at 4°C with 50 μL/well anti-mouse CD3 APC-CY7 (clone 17A2, 1:400, BD Biosciences), anti-mouse CD4 BV510 (clone RM4-5 ...
-
bioRxiv - Immunology 2021Quote: ... 10% FCS and incubated with the indicated pool of peptides (5 μg/ml final concentration of each peptide) and brefeldin A (1 μg/ml; BD Pharmingen) at 37°C for 12-16 hours ...
-
bioRxiv - Molecular Biology 2021Quote: ... run on PAGE together with input sample (1:20 of amount of immunoprecipitated proteins) and blotted with anti-PARP1 (Mouse Mab 551025 BD Pharmigen), anti-TRF1 (Rabbit Pab sc-6165 ...
-
bioRxiv - Immunology 2020Quote: ... Cells were washed once with FACS buffer and then stained with surface antibodies diluted 1:200 in Brilliant buffer (BD Biosciences) for 30 minutes ...
-
bioRxiv - Molecular Biology 2021Quote: ... Cells were re-suspended in 100 μl of FCSB and 1 μl of mouse anti-human CD10-PE (561002, BD Biosciences) and mouse anti-human CD92-AF647 (565316 ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Three stable cell lines expressing shRNAs were cultured simultaneously under 1% hypoxia and 21% normoxia for 48 hours and collected for FITC-conjugated annexin V (BD Pharmingen) staining followed by apoptotic cell number analysis by flow cytometry.
-
bioRxiv - Immunology 2022Quote: BMDMs were harvested as described before and Fc receptors were blocked with an anti-mouse CD16/CD32 antibody (1:250, BD Biosciences) in FACS buffer (5 % heat-inactivated FCS ...
-
bioRxiv - Immunology 2022Quote: ... 1–3 ×106 PBMC were stained with a saturating concentration of the following antibodies: CD3 AF700 (clone SP34-2, BD Biosciences), CD4 eFluor450 (clone OKT4 ...
-
bioRxiv - Neuroscience 2022Quote: Dissociated and resuspended iTF-Microglia were blocked for 15 min with 1:20 Human FC Block (BD Biosciences; Cat. No. 564220) and then stained with 1:66 PE/Cy7 anti-human CD184 (CXCR4 ...
-
bioRxiv - Microbiology 2022Quote: ... BW25113ΔompT (JW0554) and BW25113ΔeptA (JW5730) (Supplementary Table 1) were routinely grown aerobically in Difco Antibiotic Medium 3 (BD, Franklin Lakes, USA) at 37 °C degrees without agitation ...
-
bioRxiv - Neuroscience 2020Quote: ... Sections were incubated overnight at 4°C with the astrocyte (mouse anti-GFAP; 1:1000, BD Biosciences, San Diego, CA, USA) and microglia (rat anti-CD11b ...
-
bioRxiv - Cell Biology 2022Quote: ... 10 μl of platelet suspension was mixed with 40 μl of staining solution (HEPES-Tyrode buffer supplemented with 2.5 mM CaCl2, 1:50 PE rat anti-mouse CD41 antibody (BD Biosciences 561850), and 3:50 FITC Annexin V (BD Biosciences 556419)) ...
-
bioRxiv - Cell Biology 2022Quote: ... were loaded onto SDS-PAGE gel and immunoblotted using antibodies against clathrin heavy chain (1:1,000; BD Bioscience, San Jose, CA), dynamin (1:1,000 ...
-
bioRxiv - Immunology 2022Quote: ... PBMCs were re-stimulated with 1 µg/mL of master peptide pools in the presence of brefeldin A (GolgiPlug; BD Bioscience) and monensin (GolgiStop ...
-
bioRxiv - Immunology 2021Quote: ... stained with anti-CD117-PECy7 mAb (5 μg.mL−1) and viability dye and sorted by a FACSMelody™ cell sorter (BD biosciences). For immunofluorescence experiments ...
-
bioRxiv - Immunology 2021Quote: ... TNFα and CD107a expression by multicolor flow cytometry panel (all antibodies used are listed in Table 1) in a BD LSR Fortessa Instrument (BD Biosciences). Polyfunctionality was evaluated using Boolean gating obtained with FlowJo v10 software.