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Citations for Becton, Dickinson and Company :
6951 - 7000 of 7602 citations for 6β Fluoro 21 hydroxypregna 1 4 9 11 16 tetraene 3 20 dione 21 acetate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: Primary human bronchial epithelial basal cells (Passage 1 or 2) were expanded on flasks coated with collagen I (BD Biocoat) in bronchial epithelial growth medium (BEGM) ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by incubation in blocking buffer (1% anti-mouse-CD16/CD32, 2.4 G2, 2% FBS, 5% NRS,1% triton x100 and 2% NMS in HBSS; BD Bioscience) for 1h at room temperature ...
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bioRxiv - Immunology 2022Quote: ... Fc-blocked (1 μg/mL; eBiosciences) BALF cells were stained with anti-mouse SiglecF-PE (0.3 μg/mL; BD Pharmingen), CD11c-APC (0.3 μg/mL ...
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bioRxiv - Immunology 2022Quote: For RNAseq analysis BM cells were isolated by flushing the long bones with FACS buffer (2mM EDTA, 1% FCS, PBS) and treated with Pharm LyseTM buffer (BD). Cells were enriched by magnetic removal of CD11b+ and CD19+ cells (EasySepTM ...
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bioRxiv - Microbiology 2022Quote: 5 μl of overnight bacterial suspension (~ 1×108 cells) was plated on Columbia Agar with 5% Sheep Blood purchased from BD (Becton ...
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bioRxiv - Molecular Biology 2022Quote: ... in PBS for 60 min at RT and incubation with primary antibodies (mouse anti-RAN, 1:500, BD-610340, BD Biosciences ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by blocking with 3 % normal goat serum (NGS) in PBS for 60 min at RT and incubation with primary antibodies (mouse anti-RAN, 1:500, BD-610340 ...
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bioRxiv - Immunology 2022Quote: ... Pellets were resuspended in 50 µL cold PBS 0.25% BSA with PE-conjugated mouse anti-human IgG (1:100, BD Biosciences), or PE-conjugated goat-anti human IgA (1:800) ...
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bioRxiv - Immunology 2022Quote: ... B cells were also stained with following anti-human antibodies (all at 1:200 dilution): anti-IgD-BV650 (BD, 740594), anti-CD27-BV786 (BD biosciences ...
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bioRxiv - Systems Biology 2022Quote: ... the cell pellet resuspended in 1 ml aCSF containing 0.5% BSA and the cell suspension transferred to a round bottom tube (BD Biosciences) for flow cytometry ...
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bioRxiv - Molecular Biology 2022Quote: ... expanded for 1 week and then sorted for medium-brightness ones with a BD FACSAria III Cell Sorter (BD, USA). The proliferation rates of HEK293T control cells or cells expressing G-Flamp1 were measured using the Enhanced Cell Counting Kit-8 (Cat ...
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bioRxiv - Immunology 2022Quote: Ex vivo isolated T cells and in vitro-cultured T cells were incubated with Fc-block (1:250; BD Biosciences) followed by surface staining ...
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bioRxiv - Cancer Biology 2022Quote: ... Surface staining was performed by incubating the cell suspension with 1 μg/mL purified anti-CD16/32 (2.4G2, BD Biosciences) for 10 min at 4 °C followed by an additional 20 min incubation with appropriate dilution of the surface marker antibodies ...
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bioRxiv - Biophysics 2022Quote: ... mouse anti-flotillin-1 mAb (catalog number 610820) and mouse anti-calnexin mouse mAb (catalog number 610523) were purchased from BD Transduction Laboratories (Torrey Pines ...
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bioRxiv - Cancer Biology 2022Quote: ... Mouse cells were incubated with mouse BD Fc BlockTM containing purified anti-mouse CD16/CD32 mAb 2.4G2 at 1:400 (553142, BD BioSciences) for 10 min at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... 1µm and 2µm) were added to 1 ml of PBS in 5mL round bottom polystyrene test tubes (BD Biosciences, UK) for flow cytometry and were run on a BD FACSVerse™ (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... followed by a 20-minute incubation with a panel of directly conjugated monoclonal antibodies diluted in equal parts of fluorescence-activated cell sorting (FACS) buffer (PBS containing 0.1% sodium azide and 1% bovine serum albumin) and Brilliant stain buffer (BD Biosciences). Fluorophore-conjugated recombinant RBD protein produced by the Hensley Lab (University of Pennsylvania ...
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bioRxiv - Neuroscience 2019Quote: ... and microglia that were attached to the plate were incubated with CD11b-APC-Cy7 antibody (1:200, clone M1/70, 557657, BD) in FACS buffer (PBS supplemented with 2mM EDTA and 1% FBS ...
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bioRxiv - Neuroscience 2019Quote: ... around 12000 cells from a CD11b-enriched and CD11b-depleted fractions were stained in suspension with CD11b-APC-Cy7 antibody (1:200, clone M1/70, 557657, BD) in FACS buffer for 30 minutes at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... The primary antibodies not described in Kalyuga et al were used as follows: E-Cadherin (BD bioscience #610181, 1:10,000) and Vimentin (Leica Biosystems # VIM-572-L-CE) ...
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bioRxiv - Microbiology 2020Quote: ... Adherent and suspension cells of each sample were pooled and after centrifugation for 5min at 800 × g cells were carefully resuspended in annexin V staining buffer (RPMI1640 without phenol red containing 1 % FBS, 2 µg/ml lysostaphin, 2 mM CaCl2, 10 µl/ml annexin V-APC [BD Biosciences] and 10 µl/ml 7AAD [BD Biosciences]) ...
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bioRxiv - Immunology 2020Quote: ... Cells were maintained daily at 1 x 106 cells ml-1 and collected for flow cytometry after staining for IgG1 and B220 (FITC anti-IgG1, BD Pharmingen ...
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bioRxiv - Immunology 2020Quote: ... each sample was incubated in 1:100 dilution of rat anti-mouse unconjugated monoclonal anti-CD31 IgG (BD; CAT # 550274), and 1:50 dilution biotinylated rabbit anti-B ...
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bioRxiv - Cell Biology 2020Quote: ... PE-Cyanine7, clone eBR2a, Cat. no. 25-4321-82, eBioscience. 1:400; mouse anti-IgG2a k, FITC, clone G155-178, BD, Cat No ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed five times and developed with tetramethylbenzidine (1-Step Ultra TMB-ELISA Substrate Solution, ThermoFisher, for RBD-ELISA, and BD OptEIA Substrate Solution ...
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bioRxiv - Microbiology 2021Quote: ... Fc receptors were blocked using the anti-mouse CD16/CD32 (FcBlock clone 2.4G2) monoclonal antibodies (BD Biosciences, #553141, 1/400) and cells were stained for 30 min at 4°C with a combination of the following anti mouse immunophenotyping antibodies ...
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bioRxiv - Neuroscience 2021Quote: Primary antibodies used were as follows: Calcium Voltage-Gated Channel Auxiliary Subunit Gamma 8 (1:500; BD Transduction Laboratories 610921), Calnexin (1:500 ...
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bioRxiv - Cell Biology 2021Quote: ... After staining for 45 min at 4°C cells were washed and taken up in 200 µl PBS+2%FBS and 1/1000 7AAD before being run on a LSR Fortessa (BD). Lineage out-put was analysed in FlowJo ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed five times and developed with tetramethylbenzidine (1-Step Ultra TMB-ELISA Substrate Solution, ThermoFisher, for RBD-ELISA,and BD OptEIA Substrate Solution ...
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bioRxiv - Bioengineering 2021Quote: CHO-SCas9 cells were seeded at 0.3 × 106 cells/mL in 1 mL media in 26 wells of 12 well plates (BD Biosciences). In 25 of the wells ...
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bioRxiv - Immunology 2022Quote: ... B cells were also stained with following anti-human antibodies (all at 1:200 dilution): anti-IgD-BV650 (BD, 740594), anti-CD27-BV786 (BD biosciences ...
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bioRxiv - Immunology 2022Quote: ... 0.1 μg/ml) and CD28 (clone 37.51; 1 μg/ml) antibodies in the presence of Golgi-stop or Golgi Plug (BD Biosciences) at 37°C for 5 hrs ...
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bioRxiv - Cell Biology 2022Quote: ... For biotin conjugated antibodies (β7) the DN3a cells were rinsed and stained with BV421 Streptavidin (1:600, BD Biosciences, 563259) for one hour on ice prior to analysis.
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bioRxiv - Genomics 2022Quote: ... Cells were labeled with a 1:200 dilution of Anti-mouse CD45 antibody conjugated to BV510 (BD Biosciences, Cat#: 563891), 1:200 dilution of Anti-mouse CD31 antibody conjugated to BV510 (BD Biosciences ...
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bioRxiv - Pathology 2020Quote: RB1 IHC was performed on 12 of the 13 ULs with a mouse monoclonal antibody (Clone G3-245, 1:100, BD Biosciences Pharmingen ...
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bioRxiv - Biochemistry 2019Quote: ... The preparation refered here as HLM-1 was prepared by differential centrifugation from the sample of human liver S9 pool (lot number 3212595) obtained from BD Gentest ...
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bioRxiv - Cancer Biology 2019Quote: ... About 1×104 cells were collected and analysed for binding efficacy of the chimeras using FACS (BD FACS Calibur system).
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bioRxiv - Developmental Biology 2019Quote: ... The cells were resuspended in 30 μl PBS supplemented with Alexa Fluor 647 Mouse anti-SSEA1 (1:200, BD Biosciences) and FITC anti-mouse/rat CD61 (1:500 ...
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bioRxiv - Microbiology 2019Quote: ... Maintenance of hypoxic conditions (less than 1% O2 and greater than 13% CO2) was monitored using Dry Anaerobic Indicator Strips (BD).
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bioRxiv - Immunology 2020Quote: ... Cells were fixed in Fix perm buffer (eBioscience) for one hour followed by 1 hour in perm/wash buffer (BD). Conjugated antibodies specific for transcription factors PLZF (clone R17-809) ...
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bioRxiv - Immunology 2020Quote: ... Splenic cells were fixed with 4% paraformaldehyde and peripheral blood cells were fixed with 1% paraformaldehyde before staining for IFN-γ (XMG1.2) (eBioscience) in the presence of Perm/Wash buffer (BD) for intracellular staining ...
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bioRxiv - Immunology 2020Quote: ... was employed to investigate associations between laminin 511 expression and CX3CR1-GFP mean fluorescence intensity (MFI): cremaster muscles were stained with rat anti-mouse PECAM-1 (MEC13.3, BD Bioscience) and rabbit anti-mouse laminin α5 (antibody 405 ...
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bioRxiv - Immunology 2020Quote: ... Then cells were fixed with 2% paraformaldehyde for 1 h prior to analyzing on an LSRII flow cytometer (BD Biosciences). For cell sorting ...
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bioRxiv - Genetics 2021Quote: ... and 10 μg ml−1 propidium iodide (Fluka) at 37°C for 30 min and analysed with a FACSCalibur (BD). Cell cycle distribution data were further analysed using ModFit LT 3.0 software (Verity Software House Inc).
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bioRxiv - Cell Biology 2019Quote: Cells were stained as described and resuspended in DPBS with 1% FBS to a concentration of ∼5×106 cells/ml for ImageStream Mark II (Amnis) and ∼1×106 cells/ml for FACSAria I (BD) analysis ...
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bioRxiv - Microbiology 2021Quote: ... and the marker-specific fluorochrome-labeled antibodies rat anti-mouse CD4-AF700 (clone RM4-5, 1:200, BD cat#557956), rat anti-mouse CD8-APCy7 (clone 53-6.7 ...
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bioRxiv - Pathology 2021Quote: We used the following antibodies for immunohistochemistry (IHC) and immunoblotting (IB): Rat anti-Mouse CD31 (BD 550274; IHC 1:200), GAPDH (Cell Signaling 5174S ...
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bioRxiv - Immunology 2019Quote: ... and human monocyte-derived macrophages were seeded for 1 h at 37°C and 5% CO2 in 24- or 48-well non-tissue culture treated plates (BD) at a density of 1-2 x 105 cells/ml ...
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bioRxiv - Immunology 2020Quote: ... Cell were then further disrupted with a 1-cc syringe plunger through a 70-um nylon cell strainer (BD Biosciences), and filtered to obtain a single cell suspension ...
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bioRxiv - Systems Biology 2019Quote: ... washed in PBS and 250k cells were stained with PE-LDLR at 2 µg/ml (1:100, BD Bioscience 565653) for 20 minutes at 4°C and Zoombie NIR (BioLegend 423105 ...