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• TLR7 activation at epithelial barriers promotes emergency myelopoiesis and lung anti-viral immunity

  William D Jackson, et al., bioRxiv - Immunology 2023

  Quote: ... Monocytes were sorted into HBSS-2% FCS in 15ml Falcon tubes using a BD Aria III (BD Biosciences). Monocytes were gated as Lineage-negative (B220 ...
• A COVID-19 DNA Vaccine Candidate Elicits Broadly Neutralizing Antibodies Against Multiple SARS-CoV-2 Variants Including the Currently Circulating Omicron BF.5, BF.7, BQ.1 and XBB

  Yuan Ding, et al., bioRxiv - Immunology 2023

  Quote: ... and horseradish peroxidase (HRP)-labeled goat anti-rabbit IgG secondary Ab (BD Biosciences, San Diego, CA, USA). Chemiluminescence detection was performed with the ECL Prime Western Blotting System and acquired by the ChemiDoc Imaging System (Bio-Rad).
• A Novel MHC-Independent Mechanism of Tumor Cell Killing by CD8⁺ T Cells

  Emily Lerner, et al., bioRxiv - Immunology 2023

  Quote: Mouse-specific antibodies were purchased from BD Biosciences ...
• A Novel MHC-Independent Mechanism of Tumor Cell Killing by CD8⁺ T Cells

  Emily Lerner, et al., bioRxiv - Immunology 2023

  Quote: ... and analyzed using FlowJo v.10 (BD Biosciences).
• A novel rotavirus reverse genetics platform supports flexible insertion of exogenous genes and enables rapid development of a high-throughput neutralization assay

  Jiajie Wei, et al., bioRxiv - Microbiology 2023

  Quote: ... Staining and washing were performed in Perm/Wash buffer (BD Biosciences). Flow cytometric data were acquired using a BD LSRII flow cytometer (BD Biosciences ...
• A novel rotavirus reverse genetics platform supports flexible insertion of exogenous genes and enables rapid development of a high-throughput neutralization assay

  Jiajie Wei, et al., bioRxiv - Microbiology 2023

  Quote: ... Flow cytometric data were acquired using a BD LSRII flow cytometer (BD Biosciences) and gated on single cells ...
• Epigenetic regulation of innate immune genes and enhanced interleukin-10 expression underlie chronic subclinical Plasmodium chabaudi infection

  Leandro de Souza Silva, et al., bioRxiv - Immunology 2023

  Quote: ... using DIVA software (BD Biosciences) and analyzed by FlowJo software version 10 (FlowJo LLC ...
• Excessive apoptosis of naïve T cells resulting hyperactivation as a cause of mammalian aging

  Lingxia Wang, et al., bioRxiv - Immunology 2023

  Quote: ... CD3e (eBioscience, 25-0031-82; BD, 553061), CD4 (eBioscience ...
• Excessive apoptosis of naïve T cells resulting hyperactivation as a cause of mammalian aging

  Lingxia Wang, et al., bioRxiv - Immunology 2023

  Quote: The following antibodies were used: against mouse CD45 (BD, 553080), CD3e (eBioscience ...
• Molecular basis for potent B cell responses to antigen displayed on particles of viral size

  Jeremy F. Brooks, et al., bioRxiv - Immunology 2023

  Quote: ... anti-CD40 (1μg/ml, hm40-3 clone; BD Pharmingen), recombinant murine BAFF (20ng/ml ...
• Molecular basis for potent B cell responses to antigen displayed on particles of viral size

  Jeremy F. Brooks, et al., bioRxiv - Immunology 2023

  Quote: ... CD45.2 (104) conjugated to biotin or fluorophores (BioLegend, eBiosciences, BD, or Tonbo).
• Subset-specific and temporal control of effector and memory CD4+ T cell survival

  Sharmila Shanmuganad, et al., bioRxiv - Immunology 2023

  Quote: ... (BD Biosciences), CXCR5 ...
• A novel adjuvant formulation induces robust Th1/Th17 memory and mucosal recall responses in Non-Human Primates

  Joshua S Woodworth, et al., bioRxiv - Immunology 2023

  Quote: ... with the plunger from a 3 mL syringe (BD). Cells were washed twice in cold RPMI or PBS followed by 5 minutes centrifugation at 1800 rpm ...
• Single shot dendritic cell targeting SARS-CoV-2 vaccine candidate induces broad and durable systemic and mucosal immune responses

  Nicholas You Zhi Cheang, et al., bioRxiv - Immunology 2023

  Quote: ... 100 µL of AEC substrate (551951; BD Biosciences) was added per well and incubated for 30 mins before rinsing ten times with dH2O ...
• Single shot dendritic cell targeting SARS-CoV-2 vaccine candidate induces broad and durable systemic and mucosal immune responses

  Nicholas You Zhi Cheang, et al., bioRxiv - Immunology 2023

  Quote: ... Ancestral and variant spike expressing HEK293T cells were sorted for as target cells using the FACSAria™ Fusion Cell Sorter (BD Biosciences ...
• Human immune phenotyping reveals accelerated aging in type 1 diabetes

  Melanie R. Shapiro, et al., bioRxiv - Immunology 2023

  Quote: ... Data were acquired on an LSRFortessa (BD Biosciences) within 24 hours of staining ...
• Secretory IgM (sIgM) is an ancient master regulator of microbiota homeostasis and metabolism

  Yang Ding, et al., bioRxiv - Immunology 2023

  Quote: ... sIgT+ and sIgM/IgT++ bacteria were sorted per pool using a BD FACSAria II flow cytometer (BD Biosciences), while an aliquot of the whole bacteria suspension from each pool was stored as pre-sorted sample ...
• Human immune phenotyping reveals accelerated aging in type 1 diabetes

  Melanie R. Shapiro, et al., bioRxiv - Immunology 2023

  Quote: ... Analyses were performed in FlowJo software (v9 and v10; BD Life Sciences) according to the gating strategies in Figure S1-S6 to derive percentage of parent and geometric mean fluorescence intensity (gMFI ...
• Myeloid Zfhx3 Deficiency Protects Against Hypercapnia-induced Suppression of Host Defense Against Influenza A Virus

  S. Marina Casalino-Matsuda, et al., bioRxiv - Immunology 2023

  Quote: ... and stained with fluorochrome-conjugated antibodies (antibodies, clones, fluorochromes, and manufacturers were described in detail in our previous publication 47. Data were acquired on a BD LSR II flow cytometer (for information regarding instrument configuration and antibody panels ...
• Single-cell analysis of CX3CR1⁺ cells reveal a pathogenic role for BIRC5⁺ myeloid proliferating cells driven by Staphylococcus aureus leukotoxins

  Denis G. Loredan, et al., bioRxiv - Immunology 2023

  Quote: ... CD11b BUV395 (M1/70, BD Horizon), CD11c BV650 (N418 ...
• Tyrosine kinase inhibitors target B cells

  Valentyn Oksenych, bioRxiv - Immunology 2023

  Quote: ... Fixable Viability Stain 510 (BD Biosciences ...
• Tyrosine kinase inhibitors target B cells

  Valentyn Oksenych, bioRxiv - Immunology 2023

  Quote: ... Fixable Viability Stain 510 (BD Biosciences ...
• Insulin B peptide-MHC class II-specific chimeric antigen receptor-Tregs prevent autoimmune diabetes

  Justin A. Spanier, et al., bioRxiv - Immunology 2023

  Quote: ... anti-CD8a (PE, 53-6.7; BD Biosciences), and anti-Vβ4 (PE ...
• Insulin B peptide-MHC class II-specific chimeric antigen receptor-Tregs prevent autoimmune diabetes

  Justin A. Spanier, et al., bioRxiv - Immunology 2023

  Quote: ... CD4+ (BD Biosciences and Tonbo Biosciences), CD25+ (BioLegend) ...
• Active maintenance of CD8⁺ T cell naïvety through regulation of global genome architecture

  Brendan E. Russ, et al., bioRxiv - Immunology 2023

  Quote: ... Samples were read with a FACSCanto II cytometers (BD Biosciences), and analysed using FlowJo software (Tree Star ...
• Insulin B peptide-MHC class II-specific chimeric antigen receptor-Tregs prevent autoimmune diabetes

  Justin A. Spanier, et al., bioRxiv - Immunology 2023

  Quote: ... and anti-Vβ4 (PE, KT4; BD Biosciences). Slides were mounted with ProlongDiamond antifade reagent (P36961 ...
• Active maintenance of CD8⁺ T cell naïvety through regulation of global genome architecture

  Brendan E. Russ, et al., bioRxiv - Immunology 2023

  Quote: ... cells were fix and permeabilised according to manufacturer’s instructions (BD Biosciences) prior to staining with anti-CCL4 and CCL5 antibodies ...
• Epigenetic regulation of innate immune genes and enhanced interleukin-10 expression underlie chronic subclinical Plasmodium chabaudi infection

  Leandro de Souza Silva, et al., bioRxiv - Immunology 2023

  Quote: ... Data were acquired on an LSRII cytometer (BD Biosciences) using DIVA software (BD Biosciences ...
• Intraperitoneal transfer of wild-type bone marrow cells in the Csf1r knockout rat repopulates resident tissue macrophages without contributing to monocytopoiesis

  Anuj Sehgal, et al., bioRxiv - Immunology 2023

  Quote: ... Cells were stained for 45 min on ice in FC buffer containing unlabelled CD32 (BD Biosciences, Sydney, Australia) to block Fc receptor binding ...
• Adult thymus-derived cMaf⁺RORγt⁺ γδ T cells lack Scart2 chromatin accessibility and do not reach periphery

  Tao Yang, et al., bioRxiv - Immunology 2023

  Quote: ... anti-IFN-γ PE-Cy7 (XMG1.2, BD) and anti-IL-17A APC (TC11-18H10.1 ...
• Adult thymus-derived cMaf⁺RORγt⁺ γδ T cells lack Scart2 chromatin accessibility and do not reach periphery

  Tao Yang, et al., bioRxiv - Immunology 2023

  Quote: ... anti-CD44 BUV496 (IM7, BD), anti-CD45RB (126A ...
• Adult thymus-derived cMaf⁺RORγt⁺ γδ T cells lack Scart2 chromatin accessibility and do not reach periphery

  Tao Yang, et al., bioRxiv - Immunology 2023

  Quote: ... Flow cytometry was performed using BD LSRII (BD) equipped with three lasers operating on 405nm ...
• Adult thymus-derived cMaf⁺RORγt⁺ γδ T cells lack Scart2 chromatin accessibility and do not reach periphery

  Tao Yang, et al., bioRxiv - Immunology 2023

  Quote: Sorting was performed in the Cell Sorting Core Facility of the Hannover Medical School by using the FACSAria Fusion (BD). For all NGS experiments Vγ4 cells were FACS sorted as Zombie Aqua-GFP+ TCRβ- Vγ4+ lymphocytes.
• Adult thymus-derived cMaf⁺RORγt⁺ γδ T cells lack Scart2 chromatin accessibility and do not reach periphery

  Tao Yang, et al., bioRxiv - Immunology 2023

  Quote: ... anti-TCRβ PE-Cy5 (H57-597, BD), anti-CD3 APC-Fire810 (17A2 ...
• Adult thymus-derived cMaf⁺RORγt⁺ γδ T cells lack Scart2 chromatin accessibility and do not reach periphery

  Tao Yang, et al., bioRxiv - Immunology 2023

  Quote: ... anti-CD27 BUV563 (LG.3A10, BD) anti-CD73 BV605 (TY/11.8 ...
• Adult thymus-derived cMaf⁺RORγt⁺ γδ T cells lack Scart2 chromatin accessibility and do not reach periphery

  Tao Yang, et al., bioRxiv - Immunology 2023

  Quote: ... anti-Vγ4 BV480 (UC3-10A6, BD), anti-Vγ4 APC (UC3-10A6 ...
• Adult thymus-derived cMaf⁺RORγt⁺ γδ T cells lack Scart2 chromatin accessibility and do not reach periphery

  Tao Yang, et al., bioRxiv - Immunology 2023

  Quote: ... anti-CD45RB (126A, BD) anti-CD24 PE (M1/69 ...
• Adult thymus-derived cMaf⁺RORγt⁺ γδ T cells lack Scart2 chromatin accessibility and do not reach periphery

  Tao Yang, et al., bioRxiv - Immunology 2023

  Quote: ... anti-CD73 BV605 (TY/11.8, BD), anti-CD62L BV711 (MEL-14 ...
• Targeted cohesin loading characterizes the entry and exit sites of loop extrusion trajectories

  Ruiqi Han, et al., bioRxiv - Genomics 2023

  Quote: ... 6ug anti-BrdU antibodies (BD biosciences) pre-coupled with Dynabeads protein G beads (Thermofisher ...
• KLRG1 marks tumor-infiltrating CD4 T cell subsets associated with tumor progression and immunotherapy response

  Casey R. Ager, et al., bioRxiv - Immunology 2023

  Quote: ... Surface antibodies were prepared at optimal dilutions (see Supplementary Table S1) in FACS supplemented with Brilliant Stain Plus buffer (BD; Franklin Lakes, NJ), then were added to samples in blocking solution ...
• A Gamma-adapted recombinant subunit vaccine induces broadly neutralizing antibodies against SARS-CoV-2 variants and protects mice from infection

  Lorena M. Coria, et al., bioRxiv - Immunology 2023

  Quote: ... and visualized with tetramethylbenzidine (TMB) substrate (BD biosciences ...
• Human CCR6⁺ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticity

  Satya P. Singh, et al., bioRxiv - Immunology 2023

  Quote: ... All cell sorting was done using a FACSAria Cell Sorter (BD Biosciences). By post-sort analysis ...
• The macrophage genetic cassette inr/dtor/pvf2 is a nutritional status checkpoint for developmental timing

  Sergio Juarez-Carreño, Frederic Geissmann, bioRxiv - Immunology 2023

  Quote: ... in on an ARIA III (BD bioscience). Flow cytometry plots were obtained with Flow Jo 9.9 ...
• Salmonella cancer therapy metabolically disrupts tumours at the collateral cost of T cell immunity

  Alastair Copland, et al., bioRxiv - Immunology 2023

  Quote: ... the digestion mixture was passed through a 70 μm cell strainer (BD Falcon), washed in FACS buffer ...
• Microfluidics-enabled fluorescence-activated cell sorting of single pathogen-specific antibody secreting cells for the rapid discovery of monoclonal antibodies

  Katrin Fischer, et al., bioRxiv - Immunology 2023

  Quote: ... anti-IgG2a/2b BV421 (1:100, clone R2-40, BD), anti-IgG2a BV421 (also binds IgG2c ...
• Microfluidics-enabled fluorescence-activated cell sorting of single pathogen-specific antibody secreting cells for the rapid discovery of monoclonal antibodies

  Katrin Fischer, et al., bioRxiv - Immunology 2023

  Quote: ... anti-IgG3 BV421 (1:100 dilution, clone R40-82, BD), anti-IgM BV605 (1:40 ...
• Microfluidics-enabled fluorescence-activated cell sorting of single pathogen-specific antibody secreting cells for the rapid discovery of monoclonal antibodies

  Katrin Fischer, et al., bioRxiv - Immunology 2023

  Quote: ... Functionalised agarose beads were washed twice and then incubated with 20 nM of the GFP-streptavidin tetramers and anti-mouse IgG2a/b BV421 antibody (1:10 dilution, clone R2-40, BD Bioscience) for 30 min on ice ...
• Molecular and functional properties of human Plasmodium falciparum CSP C-terminus antibodies

  Opeyemi Ernest Oludada, et al., bioRxiv - Immunology 2023

  Quote: ... Single-cell sorting of 7AAD−PfCSP+CD19+ B cells gated positive for either IgG or CD27 as PfCSP memory B cells and 7AAD−CD38+CD27+CD19+ cells as plasmablasts was performed using a FACS Aria II (BD Biosciences) with FACSDiva software (version 8.0.1 ...
• Red2Flpe-SCON: A Versatile, Multicolor Strategy for Generating Mosaic Conditional Knockout Mice

  Szu-Hsien Sam Wu, et al., bioRxiv - Genetics 2023

  Quote: ... with a BD-LSRFortessa flow cytometer (BD). Data from the flow cytometry experiments were analyzed using FlowJo software (BD).
• The phosphodiesterase 2A regulates lymphatic endothelial development via cGMP-mediated control of Notch signaling

  Claudia Carlantoni, et al., bioRxiv - Developmental Biology 2023

  Quote: ... before the cell pellet was resuspended in 100µl Fc block (mouse BD Fc Block CD16/CD32 Clone 2.4G2 ...