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Citations for Becton, Dickinson and Company :
651 - 700 of 1901 citations for L Alanine N T Boc 2 13C 98 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Eventually 200 000 CD8+ T cells from vaccinated animals were seeded with 50 000 ovalbumin pulsed splenocytes on IFNγ ELISpot assay plates (BD Biosciences). The cells were incubated for 48 hours and the plates were developed as described by the manufacturer.
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bioRxiv - Immunology 2019Quote: ... Intracellular cytokine staining was performed to examine the production of cytokines in these CAR-T cells using Fixation/Permeabilization Solution Kit (BD Biosciences). CD107 expression was determined as described (Liu et al. ...
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bioRxiv - Microbiology 2019Quote: ... B and T-lymphocytes and CD11c+ phagocytes were sorted on a FACSAria IIu Flow Cytometry Sorter (BD Biosciences, San Jose, CA) and into 0.22 µm Spin X centrifuge tube filters (Corning ...
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bioRxiv - Immunology 2020Quote: ... Total CD4+ T cells were isolated by negative magnetic selection using a CD4 T cell isolation kit (Stemcell) and CD4+CD25hiCD127lo-neg Treg cells were sorted on a FACS Aria (BD Biosciences). Treg cells were cultured in RPMI 1640 medium supplemented with 5% Human serum ...
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bioRxiv - Molecular Biology 2019Quote: ... 20μg of total protein was loaded per lane and probed with the following primary antibodies dissolved in TBS-T: Topoisomerase IIβ (1:1000, overnight) (BD Biosciences), αTubulin (DMIA ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... according to manufacturer’s instructions and purity of isolated CD4+ T-cells was controlled on a FACSverse™ flow cytometer (BD Biosciences) using BD Tritest CD4/CD8/CD3 antibody stain (BD Biosciences) ...
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bioRxiv - Cancer Biology 2021Quote: MART-1-specific T cells from uveal melanoma biopsies were identified as previously described (13) and sorted using FACSAria III (BD Biosciences). Sorted MART1-specific T cells were co-cultured with irradiated allogenic peripheral blood leukocytes at a 1:200 ratio in AIM-V cell culture medium (Invitrogen ...
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bioRxiv - Immunology 2021Quote: Naïve CD4+ T cells were defined as CD4+ CD8- CD25- CD44- CD62L+ cells and isolated on an Aria II (BD Biosciences) cell sorter ...
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bioRxiv - Immunology 2021Quote: ... Intracellular stains were performed using anti-T-beteFluor66O (clone 4B10; eBioscience) in conjunction with a Transcription Factor Buffer Set (BD Biosciences). Samples were acquired using an LSR Fortessa or a FACSCanto II (BD Biosciences) ...
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bioRxiv - Immunology 2020Quote: ... spleen or lymph node cells in complete RMPI at a ratio of approximately 10 T cells to 1 DC in the presence of Golgi Plug (BD Bioscience). Co-cultures were incubated at 37°C ...
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bioRxiv - Immunology 2021Quote: ... and effector memory (TEM, CD3+CD8+Tet−CD62L−CD44+) T-cells were sorted from spleen on a FACS Aria-II SORP (BD Biosciences), FACS Aria III (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... Resting- or in vitro-generated effector CD4+ or CD8+ T cells (105 cells/well) were stimulated with 1 μg/ml plate-bound anti-mouse CD3 mAb (BD Bioscience) in combination with different concentrations of P3CSK4 ...
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bioRxiv - Immunology 2021Quote: ... Enzyme-linked ImmunoSpot (ELISPOT) assay was performed to measure IFN-γ+ CD8+ T cells according to the manufacturer’s protocol (BD Bioscience). CD8+ T cells were mixed with irradiated B16 cells at 1:1 ratio (250,000 cells each ...
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bioRxiv - Immunology 2021Quote: ... mice spleens were then harvested and Batf cKO and Irf4 cKO CD8+ P14 T cells were sorted with cell sorter (BD, FACSAria) according to the GFP and YFP fluorescence expression ...
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bioRxiv - Immunology 2021Quote: ... Spike -specific T cells were measured by surface staining followed by fixation and permeabilization using BD fixation/permeabilization solution kit (BD Biosciences,) and intracellular cytokine staining (table S1) ...
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bioRxiv - Immunology 2021Quote: ... Cells were then washed in 1x eBioscience Permeabilization Buffer twice and incubated with indicated intracellular antibodies including anti-T-bet-BV785 (4B10, BD Biosciences), anti-EOMES-PerCP-ef710 (X4-83 ...
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bioRxiv - Genomics 2021Quote: ... Purity of the cell cultures was determined by measuring the expression of the cardiomyocyte-specific marker cardiac troponin T (564767, BD Biosciences) by flow cytometry ...
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bioRxiv - Immunology 2022Quote: ... For quantification of the different developmental steps in T cell maturation the cells were blocked with murine Fc-block (BD Biosciences) and stained with either CD45-APC/Cy7 (BD Biosciences ...
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bioRxiv - Microbiology 2022Quote: ... CD4+ and CD8+ T cells were additionally sorted by fluorescence activated cell sorting (FACS) using an ARIA II cell sorter (BD Bioscience). For flow cytometry analyses ...
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bioRxiv - Immunology 2022Quote: Naive and effector/memory CD4+ T cells were isolated by Fluorescence-activated Cell Sorting (FACS) upon staining with anti-CD4 antibody (BD 562970), anti-CCR7 antibody (353227 BioLegend ...
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bioRxiv - Cancer Biology 2022Quote: ... CD137 expression on CD8+ T cells was assessed after an overnight co-culture with an anti-CD137 PE antibody (BD Biosciences).
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bioRxiv - Bioengineering 2022Quote: ... The specificity of CD8+ T cells towards the peptide was previously assessed by co-culture of 5·104 T cells with pulsed tumor cell lines in the presence of Golgi Plug (BD Biosciences) in 96-well plate at 1:1 E:T cell ratio.
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bioRxiv - Cancer Biology 2022Quote: Whole transcriptome analysis at single cell level was performed on FACS-sorted primary T-ALL cells using the BD Rhapsody Single-Cell Analysis System (BD, Biosciences).
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bioRxiv - Immunology 2022Quote: ... pErk1/2T202/Y204 and pAktS473 of activated CD4+ T cells was performed by fixing for 15 minutes at 37 °C with BD Cytofix (BD Biosciences), permeabilization for 30min on ice with BD Phosflow™ Perm Buffer III (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... cells were stained intracellular with anti-Foxp3 Alexa Fluor 488 (eBioscience; 1/100) and anti-ROR t BV421 (BD; 1/100). Flow cytometry data were analyzed using FlowJo Software 10.8.1 and a sequential gating strategy ...
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bioRxiv - Cancer Biology 2023Quote: For detecting intracellular PTP1B splenic CD3+CD19−CD49b−NK.1.1− T cells and CD3− CD19−CD49bhiNK.1.1hi NK cells were blocked with ant-CD16/CD32 (BD Biosciences, Cat# 553142) and then fixed and permeabilized with the BD Cytofix/Cytoperm kit according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: Cellular toxicity assays in primary T cells were performed 72 hours post-transduction using the Annexin V:PE Apoptosis Detection Kit (BD Biosciences, 559763). In brief ...
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bioRxiv - Immunology 2022Quote: ... GFP expression in peripheral blood B and T cells was screened using anti-CD19-PE (BD, clone 1D3, dilution 1/100), anti-CD3e-PerCP-Cy5.5 (eBioscience ...
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bioRxiv - Immunology 2023Quote: ... Single cell suspensions of ex vivo organs were co-cultured with DCs in complete RMPI at a ratio of approximately 10 T cells to 1 DC in the presence of Golgi Plug (BD Bioscience). Co-cultures were incubated at 37°C ...
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bioRxiv - Immunology 2023Quote: ... Cell sorting of CD8+ T cell subsets and transduced cells was performed on a BD FACS ARIA II SORP (BD Biosciences).
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bioRxiv - Immunology 2023Quote: ... Cells were subsequently stained for 20 min a 4°C with the appropriate combinations of fluorochrome-conjugated anti-human antibodies for the identification of CD8 T cells and sub-populations: CD3 BV480 (clone UCHT1, BD Biosciences), CD8 BV421 (clone RPA-T8 ...
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bioRxiv - Bioengineering 2023Quote: ... CD3+ T cell proliferation was quantified by measuring the corresponding decrease in cell fluorescence by flow cytometry (BD FACS Symphony A5.2). The experiments were performed with cells from three separate donors (donors A-C ...
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bioRxiv - Cancer Biology 2023Quote: ... CD8/CD4 T cells ratio and activation was assessed with BD Multitest CD3/CD8/CD45/CD4 (1:20; BD Biosciences, #342317) and APC-CD69 (1:100 ...
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bioRxiv - Immunology 2024Quote: ... Live NK (CD3-CD56+CD138-) and T cells (CD3+CD138-) from each donor were FACS sorted (ARIA II Fusion, BD Biosciences) and mixed at a 1:2 ratio of NK:T cells ...
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bioRxiv - Immunology 2024Quote: ... CD8+ T cells were stained and processed as described above and sorting was performed using a FACSAria III cytometer (BD Biosciences). No PBS-PFA fixation steps were performed in this case.
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bioRxiv - Neuroscience 2024Quote: Purified T cells stained with respective Abs were analyzed by three-color flow cytometry (FACScan II; BD Biosciences, San Jose, CA) to assess purity ...
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bioRxiv - Immunology 2024Quote: ... CARpos and CARneg CD3+ T-cells were FACS-sorted at high purity (>95%) using a FACS Aria flow cytometer (BD Biosciences).
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bioRxiv - Cell Biology 2019Quote: ... filtered through 40 μm cell strainer and sorted into VTN-N-coated 96-well plates using FACSAriaIII Fusion (BD Biosciences) with a 100-μm nozzle and FACSDiva software (BD Biosciences) ...
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bioRxiv - Biophysics 2021Quote: ... or from all the atoms of the channel including the N-terminal and barrel atoms (section « PB/PNP and GCMC/BD »). In all cases ...
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bioRxiv - Plant Biology 2021Quote: ... between the NdeI or NcoI and BamHI restriction sites in order to express N-terminal fusion proteins with the GAL4 DNA binding domain (BD) or with the GAL4 activation domain (AD) ...
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bioRxiv - Pathology 2020Quote: ... slides were incubated overnight at 4 °C with a 1:100 dilution of N-cadherin antibody (BD Transduction Labs, #610920) followed by incubation with secondary fluorescence antibody Alexa Fluor Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... we incubated the coverslips or plates O/N at +4 °C with 0.1 mg/ml of collagen I from rat tail (BD Biosciences) in 0.25% acetic acid ...
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bioRxiv - Plant Biology 2023Quote: ... 2007) for expression with an N-terminal LexA activation domain under the control of a constitutive ADH1 promoter (BD-NLR). Effector variants were cloned into pB42AD-GW (Shen et al. ...
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bioRxiv - Microbiology 2023Quote: ... 50 μL of the stain cocktail (1x Brillant Stain Buffer [BD Bioscience] and either 5 μL per sample of Alexa Flour 647 Anti-Total Stat1 [N-Terminus] [BD Biosciences] and 5 μL per sample of PE anti-STAT1 Phospho [Tyr701] [BioLegend] ...
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bioRxiv - Cell Biology 2022Quote: ... For nitrogen starvation in SD-N medium (0.17% (w/v) yeast nitrogen base without amino acids and ammonium sulfate (BD Difco) and 2% (w/v ...
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bioRxiv - Cell Biology 2023Quote: ... cells were grown in SDC overnight to early log-phase and then washed at least three times in synthetic defined without nitrogen (SD-N) media composed of 0.17% (w/v) yeast nitrogen base without amino acids and ammonium sulfate (BD Difco) and 2% (w/v) ...
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bioRxiv - Biochemistry 2022Quote: ... SC media contained 1.7 g/l yeast-nitrogen base with ammonium sulphate (BD Difco™), 20 g/l glucose and was supplemented with the required amino acid mixtures (Sigma ...
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bioRxiv - Microbiology 2019Quote: ... The TSA+ was prepared by adding 30 g/L of trypticase soy broth (BD, Difco), 0.98 g/L of MgSO4 ...
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bioRxiv - Synthetic Biology 2021Quote: ... (YNB; 1.7 g/l Yeast Nitrogen Base without amino acids and ammonium sulfate (BD Difco), 5 g/l ammonium sulphate ...
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bioRxiv - Microbiology 2020Quote: Gonococcal base agar was made from 10 g/l BactoTM agar (BD Biosciences, Bedford, MA), 5 g/l NaCl (Roth ...