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Citations for Becton, Dickinson and Company :
651 - 700 of 8141 citations for Anti CD4 TRX1 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... T-cells were then stained with CD4 and CDRB and sorted on a FACS Aria Fusion (BD biosciences). CD4+ CDRBhi cells were collected and administered to TCRb-/- or TCRb-/-;Clec12a-/- mice intra-peritoneally (I.P.) ...
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bioRxiv - Cancer Biology 2023Quote: ... CD4 and CD8 T cell depletion were confirmed using flow cytometry on the BD FACS CantoII (BD Biosciences) from the mouse spleen ...
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bioRxiv - Immunology 2023Quote: ... CFSE fluorescence intensity was measured in CD3+CD4+DAPI- T cells with BD FACS CANTO II (BD Biosciences) to measure the proliferation of CD4+CD25- effector T cells with or without the presence of CD8+CD45RClow/-TNFR2+ Tregs or total CD8+CD45RClow/- Tregs.
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bioRxiv - Immunology 2024Quote: ... Naive TCR7B8 T cells were sorted as CD4+CD3+CD44loCD62LhiCD25−TCRVβ14+ on the FACS Aria II (BD Biosciences). Sorted cells were then resuspended in PBS on ice and transferred into congenic isotype-labelled recipient mice by tail injection (i.v.) ...
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bioRxiv - Immunology 2024Quote: ... and CD4 (Table S3) was performed following BD Cytofix/Cytoperm Fixation/Permeabilization Kit according to manufacturer protocol (BD). For tofacitinib treatments ...
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bioRxiv - Immunology 2020Quote: ... Positively selected CD4+ T cells were labelled with CD27 FITC (O323) and CD45RA APC (HI100) (both from BD Biosciences) and sorted using a FACSAria (BD Biosciences) ...
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bioRxiv - Immunology 2020Quote: ... Infected or uninfected K562 and CD4+ T cell data was collected using the BD LSRII flow cytometer (BD Biosciences). Data were analyzed using FlowJo V10.1 software (Tree Star).
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bioRxiv - Immunology 2020Quote: The following antibodies and reagents were used for flow cytometry: CD4 clone RM-4-5 (BD Biosciences, BioLegend, Tonbo), CD8α clone53-6.7 (BD ...
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bioRxiv - Cancer Biology 2021Quote: ... CD45+CD4+CD8+ cells were sorted using a 100 µm nozzle on either a FACSAria or FACSAria Fusion (BD) and processed immediately after.
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bioRxiv - Immunology 2022Quote: ... CD3 (SP34-2, Cat# 557757), CD4 (SK3, Cat# 612749), PD1 (EH12.2, Cat# 564104), CTLA4 (BN13, 561717) were obtained from BD Biosciences ...
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Convergence, plasticity, and tissue residence of regulatory T cell response via TCR repertoire prismbioRxiv - Immunology 2023Quote: ... Effector Treg cells and effector CD4+ T cell subsets were sorted using an Aria-II Cell Sorter (BD Biosciences). Briefly ...
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bioRxiv - Immunology 2023Quote: Purified CD4+FYP+ Treg cells (1 × 105) were stimulated the plate-bound α-CD3 (1 μg/ml) (BD Pharmingen), α-CD28 (1 μg/ml ...
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bioRxiv - Immunology 2024Quote: ... CD45+CD3+CD4+CD25neg(Teffs) and CD45+CD3+CD8+ cells were sorted using FACSAria™ Fusion Flow Cytometer (BD) with 100um nozzle in KCL BRC Flow Cytometry Core ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were incubated with a lineage antibody cocktail (B220, CD3, CD4, CD8, Gr1, CD11b, NK1.1, Il7R, CD19) and were sorted on FACSAria II sorter (BD Biosciences) to recover GMPs ...
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bioRxiv - Immunology 2022Quote: ... recent thymic emigrated (CD4+CD127+CD31+, designated hereafter as naïve) T cells were obtained using a FACSAria cell sorter (BD Biosciences). The purity of the isolated cells was > 95% ...
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bioRxiv - Cell Biology 2022Quote: ... To analyse the surface expression of αE and β7 the cells were stained with BV605 CD4 (:500; BD Pharmingen, 563151), BV711 CD8 ...
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bioRxiv - Immunology 2020Quote: ... the SVCs were stained with specific antibodies (CD45, CD8, CD3, CD4, CD11c, eBioscience; CD64, BD Pharmingen, Franklin Lakes, NJ, USA) for 30 min at 4 C in the dark ...
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bioRxiv - Immunology 2021Quote: ... Committed (CD24+CD73+) or Mature (CD24loCD73+) thymocytes and CD4+CD8+ DP thymocytes were purified by FACS (BD FACS Aria III) and the RNA was isolated using RNeasy micro kit (Qiagen ...
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bioRxiv - Biophysics 2020Quote: ... The lineage depleted fraction was stained with 13 markers for lineage-specfic monoclonal antibodies FITC (CD2, CD3, CD4, CD11b, CD14, CD15, CD24, CD10, CD41, CD20, CD66c, CD127, CD33) (Biolegend and BD) and Pacific blue conjugated anti-CD34 (Biolegend) ...
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bioRxiv - Immunology 2020Quote: ... T cells were 2-way sorted from PBMC pre-gated on CD4+ CD14− viable lymphocytes using a FACS Aria (BD) and the antibodies listed in supplementary material procured from Biolegend or BD ...
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bioRxiv - Neuroscience 2023Quote: ... and T lymphocytes were stained using antibodies against CD4 and CD8 (1:100, catalog nos. 553049 and 553032; BD Biosciences). At least 1◻×◻105 leukocytes per mouse were analyzed and their amount per microliter of blood was calculated.
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bioRxiv - Immunology 2022Quote: ... Live (PI-) conventional (YFP-) and regulatory (YFP+) CD4+TCRβ+ T cells were sorted with a BD FACS Aria (BD Biosciences) to >95% purity ...
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bioRxiv - Immunology 2024Quote: ... were washed with PBS and incubated with pMHC and additional antibodies (CD3-BV421, 1:20, BD; CD8-BV650, 1:100, BD; CD4-V500, 1:50, BD; CD137-APC ...
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bioRxiv - Immunology 2024Quote: ... were washed with PBS and incubated with pMHC and additional antibodies (CD3-BV421, 1:20, BD; CD8-BV650, 1:100, BD; CD4-V500, 1:50, BD; CD137-APC, 1:25, BD; CD279-APC-Cy7 ...
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bioRxiv - Microbiology 2024Quote: ... Alexa Fluor 700), CD4 (L200, V450), CD11b (ICRF44, PE), CD16 (3G8, FITC) and CD20 (L27, PE-Cy7) all from BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... Cells were stained and live CD3+CD4+CD69+CD137+ cells were sorted and collected using a FACS Aria II (BD) (Supplementary Figure 1A).
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bioRxiv - Immunology 2024Quote: ... Tregs were then sorted on a BD FACS Aria Fusion based on viability dyenegative CD4+ (BV605, clone RM4-5, BD) GFP-FOXP3+ ...
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bioRxiv - Genetics 2023Quote: ... 10 × 106 bone marrow cells were resuspended in 200 μl of PBS supplemented with 2 % FCS containing the following antibody solution: FITC-conjugated lineage cocktail with antibodies against CD4 (clone H129.19, BD Pharmingen), CD3e (clone 145-2C11 ...
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bioRxiv - Immunology 2024Quote: Spleens from 8-10-week-old DR3 and DR3.IL17A-/- transgenic mice were harvested for CD4+ T cells by BD™ IMag Particles as per manufacturer instruction ...
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bioRxiv - Immunology 2024Quote: ... cells were washed and stained for 20 min at 4°C with mAbs CD4-APC-Cy7 (BD, clone RPA-T4), CD8-BV605 (clone RPA-T8) ...
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bioRxiv - Immunology 2024Quote: A total of 300,000 sort-purified CD4+YFP+ Treg cells from Foxp3cre and Foxp3cre Cxxc1fl/fl were resuspended in BD Pharmingen Stain Buffer (FBS) (554656; BD). Single cells were isolated using a chromium controller (BD platform ...
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bioRxiv - Immunology 2024Quote: ... and Th2 polarized (liveCD4+) CD4 T cells from WT and Pik3cdE1020K/+ mice were sorted using an Aria Fusion Sorter (BD), as previously described and then subjected to CUT&Tag using the CUT&Tag/v3 protocol (dx.doi.org/10.17504/protocols.io.bcuhiwt6 ...
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bioRxiv - Genomics 2024Quote: ... Sca1/Pe-Cy7, Flt-3/PE, CD48/FITC, CD150/BV711, Lineage (CD4, CD8, Gr1, Mac1, Ter119)/Pacific Blue and purchased from BD Biosciences or eBioscience.
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bioRxiv - Immunology 2024Quote: ... as positive markers for MSCs and CD3/4/8 (CD4/8/3, BD Tritest™; BD Biosciences San Jose, CA) CD56 (NCAM16.2 ...
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bioRxiv - Immunology 2024Quote: ... as positive markers for MSCs and CD3/4/8 (CD4/8/3, BD Tritest™; BD Biosciences San Jose, CA) CD56 (NCAM16.2 ...
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bioRxiv - Immunology 2021Quote: ... AIM assay was conducted as previously explained and CD69+CD40L+CD4+ T cells were live-sorted on a FACS Aria cell sorter (BD BioSciences) equipped for handling of biohazardous material ...
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bioRxiv - Immunology 2022Quote: ... T cell receptor Vβ chains of splenic CD4+ T cells from CKO and control mice were assessed using a commercial mouse TCR Vβ screening panel (BD Biosciences). Flow cytometry data collection was performed on an LSR II (BD Biosciences) ...
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bioRxiv - Immunology 2020Quote: ... Live CD3+CD4+CD45RA− CD25+CD127+ Teff and CD25++CD127− Treg cells were sorted with a FACSAria Fusion instrument (BD Biosciences). Sorted T cell subsets then underwent the TCRβ sequencing protocol described above ...
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bioRxiv - Immunology 2020Quote: ... Live CD3+CD4+CD45RA− activated CD154+ and resting CD154−CD69− T cells were sorted with a FACSAria II instrument (BD Biosciences). Sorted T cells were lysed in Buffer RLT Plus (Qiagen ...
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bioRxiv - Immunology 2021Quote: For Reverse Transcription PCR demonstrating 3BNC117 gene integration into the IgH locus, RNA was extracted from sorted engineered B cells (3BNC117+, CD4-, CD19+) on a FACS BD AriaIII (BD Biosciences) using the RNeasy Mini Kit (Qiagen ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... according to manufacturer’s instructions and purity of isolated CD4+ T-cells was controlled on a FACSverse™ flow cytometer (BD Biosciences) using BD Tritest CD4/CD8/CD3 antibody stain (BD Biosciences) ...
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bioRxiv - Immunology 2020Quote: ... FoxP3-GFP and ErbB2-CAR transgenic mice were then sorted on the basis of a CD4+CD8-CD62L+CD44-GFP-CD25- expression profile on a FACSAria flow cytometer (BD Biosciences). Thymic Tregs (tTregs ...
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bioRxiv - Immunology 2022Quote: ... memory B cells that bound to one or the other of these probes (CD3-CD4-CD8-CD14-CD20+IgD-IgM-IgG+probe+) were isolated with a FACSAria cell sorter using the FACSDiva software (BD Biosciences) and flow cytometric data were subsequently analyzed using FlowJo (v10.8.1) ...
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bioRxiv - Immunology 2022Quote: ... pErk1/2T202/Y204 and pAktS473 of activated CD4+ T cells was performed by fixing for 15 minutes at 37 °C with BD Cytofix (BD Biosciences), permeabilization for 30min on ice with BD Phosflow™ Perm Buffer III (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... 0.25X and 4X concentration of CD4 T cell-depleting antibody for 30 minutes at 37°C before surface staining with CD3 (clone SP34-2, BD Pharmingen), CD4 (Clone L200 ...
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bioRxiv - Immunology 2023Quote: ... Foxp3-GFP were then sorted on the basis of a CD4+CD8-CD62L+CD44-GFP-CD25- expression profile on a FACSAria flow cytometer (BD Biosciences). T cell activation was performed using plate-bound α-CD3 (clone 145-2C11 ...
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bioRxiv - Immunology 2023Quote: ... and anti-CD62L antibodies and sorted memory CD4+ T cells (CD4+ CD44hi CD62Llo) that were either GFP+ (IL-10+) and GFP- (IL-10−) by a FACSAria flow sorter (BD Biosciences).
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bioRxiv - Immunology 2023Quote: Non-pregnant Foxp3eGFP and C57BL/6 females were used to isolate peripheral CD4+ or Treg cells by cell sorting using FACS ARIA (BD Biosciences). Total lymph nodes and spleens were dilacerated ...
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bioRxiv - Cell Biology 2023Quote: ... the purity of CD4 T cells expressing the OTII T cell receptor was confirmed by flow cytometry (BD LSRFortessa Cell Analyzer) after staining with Anti-CD4-Alexa647(BD bioscience,557681 ...
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Downregulation of Let-7 miRNA promotes Tc17 differentiation and emphysema via de-repression of RORγtbioRxiv - Immunology 2024Quote: ... or CD4+CD8+ double positive cells purified from thymus single-cell suspensions by flow-cytometric sorting on FACS Aria (BD Biosciences).