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Citations for Becton, Dickinson and Company :
651 - 700 of 788 citations for 8 Benzylthio 6 oxo octanoic Acid Methyl Ester d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Cells were pelleted by centrifugation at 600 x g for 6 minutes and fixed in 100 μL per 1×106 cells of PhosFlow Lyse/Fix buffer (BD Bioscience) at 37 °C for 10 minutes ...
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bioRxiv - Immunology 2022Quote: ... and the remaining cells were incubated for 5-6 hrs at 37°C in the presence of 1 uM of the indicated peptide and brefeldin A (GolgiPlug; BD Pharmingen). Surface staining was done overnight at 4 degrees ...
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bioRxiv - Immunology 2020Quote: ... Cells were incubated for 5-6 days and CFSE dilution of T cells was determined on a BD Canto II (BD Bioscience) and analyzed by FlowJo (v10.1 ...
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bioRxiv - Immunology 2020Quote: ... fibroblast-collagen cultures were prepared in Falcon cell culture inserts and placed into Falcon 6 Well Deep Well TC-Treated Polystyrene Plates (BD Biosciences) containing 2×105 fibroblasts in FBS ...
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bioRxiv - Immunology 2021Quote: ... spleen cells of immunized mice were incubated with 50 μM of each peptide for 6 hours at 37°C in the presence of monensin (GolgiStop, BD Biosciences) and 0.8 μg of FITC-conjugated anti-mouse CD107a mAb (BioLegend) ...
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bioRxiv - Immunology 2022Quote: ... Tracheas were stretched out by pinning to SylGard-coated well of 6 well plate and fixed for 1 hr on ice in Cytofix/Cytoperm buffer (BD Biosciences). Immunofluorescent staining was performed in PBS with 0.25% Triton X-100 at 4°C as follows ...
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bioRxiv - Immunology 2022Quote: ... and 10% heat-inactivated FBS) and incubated at 37°C for 6 hours with protein transport inhibitors GolgiStop and GolgiPlug (BD Biosciences) and S glycoprotein peptide pools 1 μg/mL (JPT product PM WCPV-S-1 ...
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bioRxiv - Microbiology 2020Quote: ... from the four subgroups were co-cultured in the Biolog AN Microplate in a pairwise fashion (n =6, AB, AC, AD, BC, BD, CD), by combining 50 μL of each isolate suspended in inoculation fluid in each well ...
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bioRxiv - Microbiology 2021Quote: ... lymphocytes were cultured in 96-well dishes at 37°C for 5-6 h in the presence of 2 μM peptide pool and brefeldin A (BD Biosciences). Cells were then labeled for cell-surface markers ...
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bioRxiv - Microbiology 2021Quote: ... according to the manufacturer’s protocol and intracellular staining was performed using anti-BCL-6-AF647 (clone K112-91, 1:100, BD, cat#561525).
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bioRxiv - Bioengineering 2020Quote: ... HEK293 cells transfected with ABE plasmids were collected from 6-well plate and analyzed on Becton Dickinson LSR II (BD Biosciences) to determine GFP-positive cells ...
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bioRxiv - Cell Biology 2020Quote: ... the cell surface of freshly-prepared epidermal cells was labeled with the following antihuman protein antibodies (clone, manufacturer): FITC–conjugated CD1a (HI149) and PE–conjugated HLA-DR (G46-6, both BD Pharmingen), PE–CF594–conjugated CD3 (UCHT1 ...
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bioRxiv - Immunology 2021Quote: ... and circulating plasmablasts from 6 donor mice per group (ApoE-/- versus ApoE-/-Gpr55-/- mice) were sorted using a BD FACSAria III Cell Sorter (BD Biosciences) as described above and bulk RNA-sequencing was performed using the prime-seq protocol.26 A step-by-step protocol can be found on protocols.io (dx.doi.org/10.17504/protocols.io.s9veh66) ...
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bioRxiv - Pathology 2021Quote: ... 0.2 micron sterile filtered) using an insulin syringe (31-gauge, 6 mm length, 3/10 cc, BD Veo Ultra-Fine needle). Glucose and lactate levels were measured using a minimally invasive tail-prick method at designated timepoints post-injection.
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bioRxiv - Neuroscience 2021Quote: ... The suspended cell lysates were pipetted 6 times gently and passed through a syringe 20 times (1 ml TB Syringe, BD, USA), and avoid any bubble in the procedures ...
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bioRxiv - Microbiology 2021Quote: ... The lung infiltrated cells were cultured with purified RBD protein at 10 μg/ml for 12 h at 37 °C followed by a 6 h treatment with GolgiPlug (BD 555028). After blocking with FcγIII and FcγII receptors antibody (BD Pharmingen ...
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bioRxiv - Immunology 2020Quote: ... CD95, CD278) or intracellular proteins (BCL-2, BCL-6, CD152) and cells were acquired on an LSR II analyser (BD Pharmingen), followed by flow cytometric analysis using the FlowJo Software (FlowJo LLC ...
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bioRxiv - Immunology 2021Quote: Cells were co-cultured in R10 medium alone or in presence of K562 cells for 6 hours in the presence of anti-human CD107a (H4A3, FITC, BD Biosciences). Golgi plug and Golgi stop (BD Biosciences ...
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bioRxiv - Bioengineering 2023Quote: ... Circulating levels of IL-6 and TNF were determined using a cytokine bead array (BDTM Cytometric Bead Array Mouse IL-6/TNF Flex Set, BD Biosciences). Samples were analyzed in technical triplicates.
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bioRxiv - Neuroscience 2023Quote: ... single viable cells were gated and CD45-O1+ and CD45lowSiglec- H+ cells were collected using a FACSAria III and corresponding software (FACSDiva, v.6; BD Biosciences). Calculation of the number of CD45lowSiglecH+ microglia per brain was performed by extrapolating their frequency to the counted total number of isolated cells ...
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bioRxiv - Microbiology 2023Quote: Stm strain SL1344 with plasmid pMW211[C.10E/DsRed] [20] and strain 12023 with plasmid pluxCDABE [6] were recovered from frozen glycerol stock and cultured in Difco Luria-Bertani (LB) Broth (BD Biosciences) containing 100 µg/ml ampicillin (Merck ...
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bioRxiv - Immunology 2022Quote: ... Supernatants were recovered and the amounts of IL-6 were analyzed by cytokine-specific ELISA kits (BD Biosciences, Mountain View, CA).
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bioRxiv - Neuroscience 2023Quote: ... we resuspended the cells in 0.6 ml of cold PBS + RNase inhibitor and then filtered the cells with 100-μm cell strainers (Falcon brand, BD Biosciences). Cells were then incubated with fluorophore-conjugated primary antibodies against NeuN and Fos ...
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bioRxiv - Cell Biology 2024Quote: ... Electroporated cells were cultured in 24-well plates for 5-7 days and transferred to 6-well plates prior to selection by fluorescence-activated single cell sorting using FACS Melody (BD Biosciences).
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bioRxiv - Plant Biology 2023Quote: ... The flowers and the fixing solution were removed and replaced with 1 µg/mL DAPI (4′,6-diamidino-2-phenylindole, BD Biosciences) diluted in pollen isolation buffer before visualizing under a fluorescence microscope using a 420 nm filter (Zeiss LSM880 Inverted Confocal Microscope) ...
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bioRxiv - Cell Biology 2024Quote: ... Cleaned coverslips were removed from 70% ethanol and allowed to dry in a biosafety cabinet then were placed into the wells of a 6 well tissue culture plate and coated with 10 µg/ml fibronectin (BD Biosciences) in PBS (10.1 mM Sodium phosphate dibasic ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were then selected in puromycin (1μg/ml) 2 days after transduction and sorted for their EGFP fluorescence after 6 additional days using FACS ARIA III (BD Biosciences). Cells were grown for recovery and collected for genomic DNA extraction with DNeasy Blood and Tissue kit (Qiagen).
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bioRxiv - Genetics 2023Quote: ... we sorted up to 6 million cells for each FlowFISH replicate into 6 bins based on the fluorescence intensity of the target gene using the Influx Cell Sorter (BD Biosciences). To control for differences in staining efficiency of each cell ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were then stained for intracellular IL-6 and events were collected using the BD LSR Fortessa cell analyzer (BD Biosciences). Data was analyzed using the FlowJo Software (BD Biosciences).
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bioRxiv - Immunology 2023Quote: Non-pregnant Foxp3eGFP and C57BL/6 females were used to isolate peripheral CD4+ or Treg cells by cell sorting using FACS ARIA (BD Biosciences). Total lymph nodes and spleens were dilacerated ...
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bioRxiv - Cancer Biology 2023Quote: ... a single cell suspension was generated using trypsin and the cells were sorted based on GFP intensity into 6 equally logged bins using BD FACS Aria III (BD Biosciences). In a parallel control condition ...
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bioRxiv - Cell Biology 2022Quote: ... the endothelial cell monolayers in 6-well plates were lysed after removing center region using cell scraper (BD Falcon #35-3085) and washing with 1X HBSS (Corning #21-022-CV).
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bioRxiv - Neuroscience 2023Quote: ... single viable cells were gated and CD45highCD8+ cells were collected using a FACSAria III and corresponding software (FACSDiva, v.6; BD Biosciences). Calculation of the number of CD45highCD8+ T cells per brain was performed by extrapolating their frequency to the counted total number of isolated cells ...
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bioRxiv - Biophysics 2022Quote: ... The FITC ERF values were assigned to both 2 μm and 6 μm beads using a specific lot of FITC-FC Bead (BD Biosciences) as a calibrator with known ERF value ...
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bioRxiv - Cancer Biology 2024Quote: Live cells were incubated with 100 nM 6-FAM-dC-puromycin in complete culture medium for 30 min and analyzed by a FACSCalibur flow cytometer (BD Biosciences).
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bioRxiv - Bioengineering 2024Quote: ... or 100kDa conjugates and blood was drawn 6 hours post systemic injection via cardiac puncture and collected in K2EDTA-coated tubes (BD Biosciences). Tubes were centrifuged at 1500 g for 15 min at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... ELISA for human IL-6 and IL-10 were performed using ELISA kits (DuoSet [R&D Systems] or BD OptEIA [BD]) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... ELISA for human IL-6 and IL-10 were performed using ELISA kits (DuoSet [R&D Systems] or BD OptEIA [BD]) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... 10 μg/ml of ESAT-6 and CFP-10 full proteins (BEI Resources) + anti-human CD28/CD49d antibodies at 1 μg/ml (BD Bioscience). Samples were incubated at 37°C and 5 μg/ml of Brefeldin A (Sigma-Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... FITC Mouse Anti-Human CD45 Clone HI30 (RUO) and BV605 Mouse Anti-Human HLA-DR Clone G46-6 (RUO) (BD Biosciences) on Cytek® Northern Lights™ 2000 Flow Cytometer ...
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bioRxiv - Cancer Biology 2024Quote: ... staining mice were injected intravenously through the tail vein with 500 ul of 0.5 mg/mL brefeldin A (BFA) in PBS 6 hours before sacrifice and the staining was performed with the Fixation/Permeabilization Solution kit (BD, 554714).
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bioRxiv - Immunology 2022Quote: ... were picked from a selective plate and inoculated in 1 mL SD-UT medium [yeast nitrogen base-casamino acids (YNB-CAA) (BD Biosciences 223120) + 2% D-(+)-Glucose (Sigma G5767-500G ...
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bioRxiv - Immunology 2021Quote: ... Freshly drawn peripheral blood was collected in 5ml ethylene diamine tetra-acetic acid (EDTA) Vacutainer® tubes (BD Medical Supplies, Crawley, UK). PBMCs were isolated by layering 3ml of anti-coagulated blood over 3ml endotoxin-free Ficoll-Paque (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... 1 mM ethylenediaminetetraacetic acid (EDTA)) with the following anti-human antibodies (all at 1:200 dilution): anti-CD20-PECy7 (BD Biosciences, 335793), anti-CD3-APC-eFluro 780 (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... Synthetic Low Ammonium Dextrose (SLAD) broth was made by combining 0.67 g Yeast Nitrogen Base without amino acids and ammonium sulphate (BD DifcoTM, Herlev, Denmark), 2 g glucose and 50 µl of ammonium sulphate solution (1M ...
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bioRxiv - Immunology 2022Quote: ... 1 mM ethylenediaminetetraacetic acid (EDTA)) with the following anti-human antibodies (all at 1:200 dilution): anti-CD20-PECy7 (BD Biosciences, 335793), anti-CD3-APC-eFluro 780 (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... Cell suspensions were stained with the following antibodies in FACS buffer (1% fatty acid-free BSA + 1mM EDTA in PBS) for 25 minutes on ice: Fixable Viability Stain BV510 (BD Biosciences, 564406), CD11b-BV421 (BioLegend 101251) ...
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bioRxiv - Immunology 2022Quote: ... 1 mM ethylenediaminetetraacetic acid (EDTA)) with the following anti-human antibodies (all at 1:200 dilution): anti-CD20-PECy7 (BD Biosciences, 335793), anti-CD3-APC-eFluro780 (Invitrogen ...
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bioRxiv - Immunology 2021Quote: Testing for GC and CT in first-void urine samples was performed by nucleic acid amplification test (NAAT; ProbeTech Assay, BD, Sparks, MD). Testing for HIV-1/2 and syphilis were performed by chemiluminescent microparticle immunoassay (CMIA ...
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bioRxiv - Immunology 2022Quote: ... Blood was collected in BD Microtainer tubes with ethylenediaminetetraacetic acid or lithium heparin (Becton, Dickinson and Company, Franklin Lakes, New Jersey, USA) for complete blood counts (CBC ...