3' Methyl 1 1' biphenyl 4 amine citations for Becton, Dickinson and Company

Quote: 2 × 106 cells were stimulated for 4 h at 23°C with 40 ng ml−1 PMA and 1 μg ml−1 ionomycin in the presence of 2 μM Monensin (Golgi-Stop, BD Biosciences, Cat: 51-2092KZ). Cells were washed twice with FACS buffer and stained for extracellular markers ...

AMPKα1 is essential for Glucocorticoid Receptor triggered anti-inflammatory macrophage activation

Giorgio Caratti, et al., bioRxiv - Physiology 2020

Quote: ... and thereafter stained with anti-CD45 antibody (eBioscience, 25-0451-82, 1:200), anti-Ly6C antibody (eBioscience, 12-5932-82, 1:400) and anti-CD64 antibody (BD Pharmingen, 558539, 1:100) for flow cytometry analysis.

ACTIN Anchors the Highly Oligomeric DRP1 at Mitochondria-Sarcoplasmic Reticulum Contact Sites in Adult Murine Heart: Its Functional Implication

Celia Fernandez-Sanz, et al., bioRxiv - Physiology 2021

Quote: ... A 3% BSA-0.2% Triton®X-100 solution was used for permeabilization and blocking followed by a 1 h RT incubation with PBS–1% BSA containing mouse monoclonal DRP1 antibody (611113, BD Transduction Laboratories, 1:50) followed by a 1 h RT incubation with PBS–1%BSA containing rabbit polyclonal RyR2 (PA5-36121 ...

High-content phenotypic screen to identify small molecule enhancers of Parkin-dependent ubiquitination and mitophagy

Roberta Tufi, et al., bioRxiv - Cell Biology 2022

Quote: ... containing conjugated antibodies (Alexa 647 anti-p-Ser65-Ub [CST, 62802, 1:1500], Alexa 488 anti-HSP60 [Abcam, ab128567, 1:1000], and Alexa 555 anti-LAMP1 [BD Biosciences, 555798, 1:1000]) using a Dragonfly Discovery liquid dispenser and incubated at 4°C overnight in the dark with gentle agitation.

High-content phenotypic screen to identify small molecule enhancers of Parkin-dependent ubiquitination and mitophagy

Roberta Tufi, et al., bioRxiv - Cell Biology 2022

Quote: ... containing all conjugated antibodies (Alexa 647 anti-p-Ser65-Ub [CST, 62802, 1:1500], Alexa 488 anti-HSP60 [Abcam, ab128567, 1:1000], and Alexa 555 anti-LAMP1 [BD Biosciences, 555798, 1:1000]) using the Dragonfly Discovery liquid dispenser and incubated at 4°C overnight in the dark with gentle agitation.

Functional characterization of putative ecdysone transporters in lepidopteran pests

George-Rafael Samantsidis, et al., bioRxiv - Genetics 2022

Quote: ... Caspase-3 activity was calculated using the Caspase-3 assay kit (BD biosciences) following the instructions of the manufacturer ...

Induction of a CD8 T cell intrinsic DNA damage and repair response is associated with clinical response to PD-1 blockade in uterine cancer

Yuki Muroyama, et al., bioRxiv - Immunology 2022

Quote: ... Caspase 3 staining was performed using PE Active caspase 3 Apoptosis Kit (BD) as per manufacturer’s instructions ...

Permanent lymphocyte subset elimination upon a single dose of AAV-delivered depletion antibody dissects immune control of chronic viral infection

Anna Lena Kastner, et al., bioRxiv - Immunology 2024

Quote: ... Tim-3 (5D12/TIM-3) were used for staining and purchased from BD, Milteny ...

γδ T cells are effectors of immune checkpoint blockade in mismatch repair-deficient colon cancers with antigen presentation defects

Natasja L. de Vries, et al., bioRxiv - Cancer Biology 2021

Quote: ... and stained with cell surface antibodies (1:100 anti-CD137-APC [clone 4B4-1, BD Biosciences] ...

Large-scale mapping of positional changes of hypoxia-responsive genes upon activation

Koh Nakayama, et al., bioRxiv - Cell Biology 2021

Quote: ... cells were incubated for 1 h with primary antibodies (anti-HIF-1α, 1:100, BD Bioscience #610958 ...

Limited Environmental Serine Confers Sensitivity to PHGDH Inhibition in Brain Metastasis

Bryan Ngo, et al., bioRxiv - Cancer Biology 2020

Quote: ... 2.5×105 cells in 100ul of PBS were mixed 1:1 with Matrigel (BD Biosciences) and injected into the fourth mammary fat pad ...

Cell – ECM interactions play distinct and essential roles at multiple stages during the development of the aortic arch arteries

Michael Warkala, et al., bioRxiv - Developmental Biology 2020

Quote: ... The following antibodies were used 1° antibodies: anti-Pecam1 (1:200, BD Pharmingen, cat #550274), anti-VEGFR2 (1:200 ...

Drug Conjugates of Antagonistic RSPO4 Mutant For Simultaneous Targeting of LGR4/5/6 for Cancer Treatment

Jie Cui, et al., bioRxiv - Pharmacology and Toxicology 2021

Quote: ... were subcutaneously inoculated with 5 × 106 cells in 1:1 mixture with Matrigel (BD Biosciences). After 3 weeks ...

Expansion of CD10^neg neutrophils and CD14⁺HLA-DR^neg/low monocytes driving proinflammatory responses in patients with acute myocardial infarction

Daniela Fraccarollo, et al., bioRxiv - Immunology 2021

Quote: ... anti-F4/80 (clone BM8, 1:100, BioLegend; clone T45-2342, 1:100, BD Biosciences); anti-CD11b (clone M1/70 ...

OXPHOS Promotes Apoptotic Resistance and Persistence in T_H17 cells

Hanna S. Hong, et al., bioRxiv - Immunology 2021

Quote: ... in the presence of brefeldin A (1:1,000; eBioscience) and monensin (1:1,000; BD Biosciences) for 4 hours prior to intracellular staining.

Gigaxonin is required for intermediate filament transport

Bhuvanasundar Renganathan, et al., bioRxiv - Cell Biology 2022

Quote: ... SH-SY5Y neuroblastoma cells were cultured in a 1:1 mixture of EMEM (BD, #670086) and F12 (Gibco ...

Transcriptomic landscape of early hair follicle and epidermal development

Ana-Marija Sulic, et al., bioRxiv - Developmental Biology 2022

Quote: ... pH 7.2 in PBS and stained with rat anti-MAdCAM-1 (1:10, BD Pharmingen) for 10 min ...

Histologic variants in bladder cancer harbor aggressive molecular features including TM4SF1 expression and a CA125+ cell state

Heiko Yang, et al., bioRxiv - Cancer Biology 2024

Quote: ... The injected cells were resuspended in 1:1 serum-free media and Matrigel (BD Biosciences). Mice were enrolled into treatment groups once tumor volumes reached between 50-100 mm3 ...

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