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Citations for Becton, Dickinson and Company :
6901 - 6950 of 7309 citations for 6 Methyl benzo 1 3 dioxole 5 carbaldehyde since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... The permeabilized cells were stained with intracellular cytokine staining antibodies for 20 min at room temperature in 1 perm/wash buffer (BD Biosciences). Cells were then washed twice with perm/wash buffer and once with staining buffer before acquisition using the BD Symphony Flow Cytometer and the associated BD FACS Diva software ...
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bioRxiv - Immunology 2022Quote: ... Cells were resuspended in 1% para-formaldehyde until acquisition on a BD Biosciences Symphony cytometer and analyzed using FlowJo (BD Biosciences). Caspase 3 staining was performed using PE Active caspase 3 Apoptosis Kit (BD ...
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bioRxiv - Cancer Biology 2022Quote: ... Immunohistochemistry (IHC) was performed on FFEP blocks using mouse monoclonal antibodies SMARCB1/INI1 (Clone 25/BAF47, dilution 1:200, BD Bioscience) and CD70 (Clone 301731 ...
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bioRxiv - Genomics 2022Quote: ... This was followed by the secondary labeling step with 1:200 dilution of Streptavidin PE-Cy7 antibody (BD Biosciences, Cat#: 557598). Cells were isolated and sorted using an Influx Cell Sorter Instrument (BD Biosciences ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Tissues were incubated at 4°C overnight with the following primary antibodies: monoclonal mouse anti-carboxyl-terminal binding protein 2 (CtBP2) IgG1 at 1:200 (612044; BD Biosciences) counterstained with goat anti-Mouse IgG1 conjugated with Alexa Fluor 568 (#A-21124) ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed twice with 500 μL permeabilization buffer (from kit described above) and stained with 150 μL permeabilization buffer containing 1:400 Ki67-Alexa-Fluor antibody (561126, BD Biosciences) for 30 minutes at 4 °C ...
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bioRxiv - Immunology 2019Quote: ... LN or spleen cells were stimulated with 50 ng/ml PMA and 1 μM ionomycin in the presence of Golgi-Stop (BD Biosciences) for 5 h ...
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bioRxiv - Neuroscience 2019Quote: ... Supernatant was aspirated and the cell pellet was resuspended in 200uL of FACS buffer (PBS, 0.5% BSA, 2mM EDTA, 20mM Glucose) with 1:50 PE Rat Anti-Human CD49f antibody (BD Biosciences; 555736) and incubated on ice for 20 minutes ...
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bioRxiv - Immunology 2019Quote: ... harvested CD4+ T cells or lung single cells were stimulated with PMA (50 ng/ml) and ionomycin (1 µM) in the presence of GolgiStop (BD Pharmingen) for 6hr before FACS analysis.
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bioRxiv - Microbiology 2019Quote: ... Intrahaemocoelic injection of the larvae (n=10 per condition) was performed by a 1 mL tuberculin syringe (Norm-Ject) fitted with a custom 30½-gauge needle (BD PrecisionGlide) at a volume of 20 µL (∼20,000 cells ...
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bioRxiv - Cell Biology 2019Quote: ... VCAM-1 expression and E-selectin expression were detected by using APC-conjugated monoclonal antibodies anti-CD106 (VCAM-1, clone 51-10C9) and anti-CD62E (E-selectin, clone 68-5H11, BD Biosciences) respectively ...
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bioRxiv - Microbiology 2019Quote: ... Cells were incubated with 100 μg.mL−1 RNase A and 15 μg.mL−1 propidium iodide (PI) for 30 min before PI was detected using the BD FACSCalibur™ (BD Biosciences). Data was analysed using FlowJo_V10™ software (FlowJo ...
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bioRxiv - Cell Biology 2019Quote: ... 1-2×106 cells were incubated for 15 min at +4°C with blocking buffer (1xPBS, 1% BSA, 2mM EDTA) containing 5mg/ml α-CD16/CD32 (2.4G2, BD Biosciences). For surface staining ...
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bioRxiv - Immunology 2020Quote: ... The cells were washed once with FACS buffer and fixed with 2% paraformaldehyde for 1 h prior to analyzing on an LSRII flow cytometer (BD Biosciences). In some experiments ...
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bioRxiv - Immunology 2020Quote: ... Intracellular staining of IL-2 followed the same protocol as that for CTLA-4 but included the addition of 1 μg/mL GolgiPlug (BD Biosciences) to the culture media 12 hours prior to sample preparation ...
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bioRxiv - Immunology 2020Quote: The cell supernatant was collected after centrifugation of the cells and the secreted IL2 in the diluted cell culture medium (1:4 dilution) was measured using the IL2 Flex set cytometric bead array (CBA) (BD, 558270) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... samples were processed as previously described47 and 1-2 million cells were injected into cleared mammary fat pads in 10-20 microliters of commercial Matrigel (BD Biosciences). For ER+ tumors ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed once using PBS with 1% BSA and kept on ice until FACS sorting using the FACSAria III (BD Biosciences). For RNA isolation ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were then grown for 3 d and live cells were labeled with CD44-Alexa-Fluor-647 or TfR1-PE antibodies in 2% FBS/1× PBS with 1 mM ETDA and subsequently CD44-negative or TfR1-negative cells were sorted by FACS (Aria IIu, BD Biosciences), seeding one cell per well into 96-well plates ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were stained by replacing the solution containing ethanol with another made up of PBS/FBS and implemented with PI (1:100, BD Bioscience) and RNaseA (1:400 ...
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bioRxiv - Microbiology 2020Quote: ... Spore suspensions were prepared by plating a 1:100 dilution of an overnight culture onto a 70:30 medium (63 g Bacto peptone (BD Difco), 3.5 g proteose peptone (BD Difco) ...
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bioRxiv - Molecular Biology 2020Quote: ... The membranes were incubated overnight with different anti-aSyn primary antibodies with different binding properties: either 1:1000 mouse anti-aSyn (BD Biosciences), 1:3000 mouse anti-aggregated-aSyn (clone 5G4 ...
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bioRxiv - Bioengineering 2021Quote: ... We incubated the ECs in a 1:500 primary antibody solution containing Mouse anti-CD144 (VE-Cad, BD Pharmingen Cat. #55561) in ADB overnight at 4 oC ...
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bioRxiv - Cancer Biology 2021Quote: ... and cells incubated for 30 min on ice with following antibodies: PE-conjugated anti-CD11b (M1/70, 1:3000, BD Biosciences), APC-conjugated anti-F4/80 (for macrophages ...
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bioRxiv - Immunology 2020Quote: ... cells were prepared as described for flow cytometry and then sorted into FBS containing media (RPMI 1640, 20% FBS, 1% HEPES, 100 mg/mL penicillin/streptomycin) on a FACSAria II (BD Biosciences).
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bioRxiv - Immunology 2021Quote: ... for 10 min followed by staining with antibodies: 1 μl of PE-conjugated anti-mouse Ly-6G (BD Pharmingen, CA, USA), 1 μl of FITC-conjugated anti-mouse CD206 (Biolegend ...
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bioRxiv - Immunology 2020Quote: We centrifuged the samples and resuspended each in 1 mL of an antibody master mix consisting of Brilliant Buffer (BD Biosciences) and anti-CCR6 - PECy7 (BioLegend ...
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bioRxiv - Immunology 2019Quote: ... spinal cord tissue was homogenized with 1X HBSS in the presence of Golgi Plug (1:1000, BD Biosciences, San Jose, CA) and passed through nylon mesh filter for further dissociation ...
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bioRxiv - Physiology 2020Quote: ... 1 ng/mL FGF2 and 10 mg/mL gentamycin) and plated on dishes previously coated with 2% Matrigel™ (BD biosciences).
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bioRxiv - Molecular Biology 2019Quote: ... 20μg of total protein was loaded per lane and probed with the following primary antibodies dissolved in TBS-T: Topoisomerase IIβ (1:1000, overnight) (BD Biosciences), αTubulin (DMIA ...
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bioRxiv - Microbiology 2020Quote: ... xanthus cells were grown in liquid CYE medium (10 mM MOPS pH 7.6, 1% (w/v) Bacto™ casitone (BD Biosciences), 0.5% yeast extract and 4 mM MgSO4 ...
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bioRxiv - Systems Biology 2019Quote: ... Cells were washed with PBS-T and incubated with the Alexa-fluor 647 cleaved PARP primary and Alexa-fluor 488 goat anti-rabbit secondary antibodies (1:500 dilution, BD Bioscience) overnight at room temperature ...
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bioRxiv - Immunology 2021Quote: Interleukin-8 secretion of treated THP-1 monocytes was quantified by the ELISA OptEIA™ Set for human interleukin-8 (BD) following the manufacturer’s protocol with optimized wash buffer composition (50x dilution) ...
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bioRxiv - Cell Biology 2021Quote: ... cells were pelleted and washed twice with 1% FBS in PBS and subjected to flow cytometry using a FACSMelodyTM (BD Biosciences).
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bioRxiv - Physiology 2021Quote: INS-1 cells were collected for flow cytometry analysis using FITC Annexin V apoptosis detection reagent (BD Biosciences, San Jose, CA). The apoptotic cells were measured by FACS Count™ Flow Cytometer (BD Biosciences) ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed with 1 × PBS (30 s) and stained on ice in the dark for 30 min with anti-CD3 (BD, 557705), anti-CD4 (BioLegend ...
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bioRxiv - Plant Biology 2020Quote: ... and diluted to a final concentration of 1.5 × 105 spores ml−1 in half strength potato dextrose broth (PDB ½, BD Difco™) prior to inoculation ...
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Paracrine signalling between intestinal epithelial and tumour cells induces a regenerative programmebioRxiv - Cancer Biology 2021Quote: ... The sections were then blocked and permeabilized with 5% Donkey serum 0.01% Triton X-100 for 30 min at RT before being incubated with rabbit anti-YAP dilution 1/100 (Signalling Technology #14074) and mouse anti beta-catenin (BD 610153) (dilution 1/200 ...
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bioRxiv - Immunology 2021Quote: ... cells were previously stimulated with 100 ng/ml of PMA and 1 μg/ml of Ionomycin for 1h at 37°C and then incubated in GolgiStop and GolgiPlug (both from BD PharMingen) for 2 h at 37°C ...
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bioRxiv - Cell Biology 2020Quote: The following monoclonal antibodies (Abs) were used for flow cytometry and cell sorting: anti-c-Kit (2B8, BD Biosciences, 1:100), -Sca-1 (E13-161.7 ...
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bioRxiv - Developmental Biology 2021Quote: Mouse femur single cells were obtained as described above in the flow cytometry section and stained with biotin-conjugated antibodies for Lin (Ter119, Gr-1, B220, CD3e) (BD, 559971) followed by negative selection using streptavidin-coated magnet beads (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... bone marrow cells were subjected to red blood cell lysis and stained with fluorochrome-conjugated anti-mouse antibodies to CD11b (Mac-1, BD Biosciences) and/or Ly6G (1A8 ...
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bioRxiv - Cancer Biology 2021Quote: MART-1-specific T cells from uveal melanoma biopsies were identified as previously described (13) and sorted using FACSAria III (BD Biosciences). Sorted MART1-specific T cells were co-cultured with irradiated allogenic peripheral blood leukocytes at a 1:200 ratio in AIM-V cell culture medium (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: ... MDA-MB- 231-GFP cells (1 × 106) were resuspended in 100 μL of 20% of collagen I (BD Biosciences, cat #354234). Cells were injected in the 4th lower left mammary fat pad of the mouse ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were fixed in 1% formalin and analyzed on a BD FACS Canto II running the FACS Diva Software (BD Biosciences). Fluorescence values were normalized to an unstained control samples and initial compensation applied using IgG-APC control antibody (Miltenyi Biotech ...
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bioRxiv - Microbiology 2020Quote: ... Footpads were digested with 4 mg ml−1 collagenase D for 2 h at 37°C before dissociation through 70 μm cell strainers (BD Biosciences). Homogenates were resuspended in HOMEM supplemented with 20% FCS and serial dilutions (2-fold ...
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bioRxiv - Microbiology 2020Quote: ... albicans MTL a/α clinical isolate SC5314 72 was used for all experiments and was routinely grown in YPD (1% yeast extract, 2% peptone, 2% glucose; all BD Biosciences) at 30 °C.
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bioRxiv - Immunology 2021Quote: ... stained cells were resuspended in 1% paraformaldehyde (PFA) and acquired using Stained cells were acquired on a BD LSRFortessa (BD Biosciences) and analysis performed using FlowJo v10.0.8 (Tree Star ...
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bioRxiv - Immunology 2021Quote: ... and cell surface markers were stained with the following anti-mouse antibodies at 1:200 dilution per 106 cells: Ly6C (AL-21; BD Biosciences), Ly6G (1A8 ...
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bioRxiv - Immunology 2020Quote: ... spleen or lymph node cells in complete RMPI at a ratio of approximately 10 T cells to 1 DC in the presence of Golgi Plug (BD Bioscience). Co-cultures were incubated at 37°C ...