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Citations for Becton, Dickinson and Company :
6551 - 6600 of 7309 citations for 6 Methyl benzo 1 3 dioxole 5 carbaldehyde since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... resuspended in surface staining mix containing FACS buffer with 1/2 of final volume of Brilliant Stain Buffer (BD), 50 μg/ml anti-mouse CD16/CD32 (2.4G2 ...
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bioRxiv - Cancer Biology 2023Quote: ... sorted based on Lgr5 and Sca-1 expression (and absence of DRAQ7) using the FACS Aria III (BD Biosciences). Sorted cells were embedded as single cells in Collagen I gels at a density of 200 cells/μl in 96-well imaging plates (Cellstar ...
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bioRxiv - Bioengineering 2023Quote: ... labeled wild-type HUVECs in a 1:1 ratio and seeded at a final density of 7.5 X 104 in 24-well plates coated with collagen 1 (from rat tail, BD). After culturing for 48h in complete medium at 37°C and 5% CO2 ...
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bioRxiv - Neuroscience 2023Quote: ... Digested suspensions were subsequently triturated by sterilized syringes with 20G 1/2 needle (BD Biosciences, Franklin Lakes, NJ, USA) and washed with DMEM to harvest mononuclear cells ...
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Treg cells drive MYCN-mediated immunosuppression and tumor aggressiveness in high-risk neuroblastomabioRxiv - Cancer Biology 2023Quote: ... and then stained with Alexa Fluor 647 conjugated anti-FOXP3 mAbs (1:100, Clone 259D/C7, BD Biosciences, 560044) to identify CD4+CD25+FOXP3+ Treg cells ...
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bioRxiv - Immunology 2024Quote: ... CFSE-labelled cells were incubated under R10 complemented medium with 1 μg/ml of anti-CD28/CD49d (BD, Pharmigen) in the following conditions ...
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Autophagy suppression in DNA damaged cells occurs through a newly identified p53-proteasome-LC3 axisbioRxiv - Cell Biology 2024Quote: ... and then resuspended in 1% BSA in PBS pH 7.4 at the desired volume for subsequent flow cytometry analyses (LSR II analyzer, BD). 10,000 cells were measured for each analysis ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed once and stained with the following antibodies: anti-VCAM-1 (BD biosciences 551146, clone 51-10C9) anti-ICAM-1 (BD biosciences 551146 ...
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bioRxiv - Cancer Biology 2024Quote: ... female athymic nude mice were injected into the mammary fat pad with 1×106 MDA-BR cells in a 100μl mixture composed of Matrigel (50μl; BD Matrigel 10mg/ml, BD Biosciences) and PBS (50μl) ...
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bioRxiv - Microbiology 2024Quote: ... plates were washed and incubated with detection antibody and enzyme reagent for 1 h at room temperature (BD Bioscience). TMB (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... resuspended in 100 μl of FACS buffer and incubated with an anti-CD32 antibody (1:20, BD Biosciences #550271) for 30 min at 4°C to block nonspecific Fc receptor binding ...
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bioRxiv - Microbiology 2024Quote: ... homogenates were serially diluted up to 1:10,000 and 10µl of each dilution was plated onto one LB agar (BD-Difco) plate (concentrations plated ...
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bioRxiv - Immunology 2024Quote: ... and then for a further 2 hours with the addition of 1 mg/ml Brefeldin and Monensin (BD Biosciences) in complete medium ...
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bioRxiv - Immunology 2024Quote: ... The proportion of THP-1 cells containing fluorescent-positive beads was evaluated by flow cytometry (FACS CantoII, BD Biosciences), analyzed using FlowJo software and presented as phagocytosis index (the percentage of THP-1 monocytes with ingested merozoites or beads).
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bioRxiv - Cancer Biology 2021Quote: ... eBioscience] for 45 min at 4°C together with different additional antibodies for immunophenotyping (including 1:10 anti-CD103-FITC [clone Ber-ACT8, BD Biosciences] ...
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bioRxiv - Immunology 2021Quote: ... co-culture supernatants were thawed at room temperature for 1 hr and cytokines stained with mouse Th1/Th2/Th17 cytokine kit (BD) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... BALF was collected by pumping 1 ml of sterile PBS into the lungs and recovered with a syringe (BD #309659). This process was repeated once to collect 2 mL of BALF.
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bioRxiv - Genomics 2020Quote: ... DNA denaturation was achieved with 0.5% BSA and 2M HCl after which cells were incubated in 1:2000 solution of anti-BrdU (BD Pharmingen) for 1h at RT ...
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bioRxiv - Immunology 2020Quote: ... 2mM EDTA and 0.05% NaN3): PerCP-Cy™5.5 mouse anti-human CD19 (1:40 dilution, Clone HIB19, BD Pharmingen) (for PBMC samples only) ...
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bioRxiv - Bioengineering 2022Quote: ... Brooks Lifesciences) were coated with 200 μL of 2 mg/mL type 1 rat tail collagen (354249, Corning/BD Biosciences) diluted in DMEM equilibrated in 1/10 v/v of 7.5% w/v sodium bicarbonate solution (#25080-060 ...
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bioRxiv - Neuroscience 2021Quote: ... the cell pellet resuspended in 1 ml aCSF containing 0.5% BSA and the cell suspension transferred to a round bottom tube (BD Biosciences) for flow cytometry ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated with primary antibody in incubation buffer overnight at 4°C with mouse anti-CtBP2 antibody (1:50, BD Transduction ...
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bioRxiv - Neuroscience 2021Quote: ... For FACS sorting the cells were stained with anti- CD31 PE conjugated antibody in a concentration of 1:20 (#566125, clone MBC78.2, BD Pharmingen) and anti-CD45 APC conjugated antibody in a concentration of 1:20 (#17-0459-42 ...
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bioRxiv - Molecular Biology 2020Quote: ... catheter access was achieved by placing conscious animals in a rodent restrainer and using 1 mL tuberculin slip-tip syringes (cat. no. 309626, BD) with attached blunt 22 ga dispensing needles (cat ...
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Single-cell analysis of skeletal muscle macrophages reveals age-associated functional subpopulationsbioRxiv - Cell Biology 2022Quote: ... mononuclear cell suspensions were incubated with BD Horizon™ Fixable Viability Stain 780 (FVS780, BDBiosciences, Cat# 565388, Dilution: 1:4000) in PBS (Ca+ and Mg+ free ...
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bioRxiv - Evolutionary Biology 2022Quote: ... BV421-conjugated anti-rat CD45RA (clone OX-33, Cat#740043), and BV605-conjugated anti-rat CD45 (clone OX-1, Cat#740515) from BD Biosciences (Franklin Lakes ...
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bioRxiv - Developmental Biology 2022Quote: ... A density of 100,000 cells per well was used for culture in 8-well #1 coverglass chambers (Labtek) precoated with growth factor reduced Matrigel (80µl, BD Biosciences) for 30 minutes at 37°C ...
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bioRxiv - Immunology 2021Quote: ... Vα7.2+ cells were pre-incubated for 1 h and 30 min with LukED at 0.312 μg/mL before adding to the bacteria-pulsed THP-1 and co-culture at 2:1 ratio for 24 h in the presence of 1.25 μg/mL anti-CD28 mAb (L293, BD Biosciences). Monensin and brefeldin A (both from BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... Cells were plated at 106 cells/ml in 6-well plates plate-coated with anti-CD3 (clone 2C11) and anti-CD28 (clone 37.51) (1 μg/ml each, BD Biosciences) by pre-coating the wells with 100 μg/ml goat anti-hamster IgG (Cappel).
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bioRxiv - Immunology 2021Quote: ... fixed in PBS with 1% paraformaldehyde and analyzed by flow cytometry (BD Accuri™ C6 Plus Flow Cytometer, BD Biosciences), measuring cell-surface CD4 ...
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bioRxiv - Immunology 2019Quote: Indirect immunofluorescence was performed on frozen fixed (acetone:methanol) liver tissue using monoclonal antibody CD45-FITC (1:100) (30-F11, BD Pharmingen). Co-localisation was assessed using CD147 (1:100 ...
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bioRxiv - Cancer Biology 2019Quote: ... SSEA3 conjugated to Phycoerythrin and TRA-1-60 conjugated to Alexa Fluor™ 647 (all of them from BD Biosciences) for 30 minutes at 4°C ...
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bioRxiv - Bioengineering 2019Quote: ... the monocytes were incubated for 10 min at room temperature with human BD Fc-block (2.5 µg per 1×106 cells in 100 µL, Cat. no. 564220, BD biosciences). The cells were further incubated with the following antibody cocktail for 30 min at 4°C ...
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bioRxiv - Bioengineering 2019Quote: ... or saline were infused directly into the tumor at a flow rate of 10?μl min-1 via a Genie Touch syringe pump through PE10 catheter tubing and a 30-gauge needle (BD). Injection at the focal zone was confirmed via B-mode imaging by locating the needle tip ...
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bioRxiv - Developmental Biology 2020Quote: ... Membranes were blocked with BSA and incubated with primary antibodies diluted 1/1000 against Cdh5/VE-cadherin (BD Biosciences 555289), Phospho-Akt (Cell Signalling ...
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bioRxiv - Cancer Biology 2019Quote: Effect of Grem-1 in the invasion of cells were assayed using the BD BioCoat Matrigel Invasion Chambers (BD Bioscience). Each well of a 24-well plate contained an insert with an 8-mm pore size PET (polyethylene terephthalate ...
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bioRxiv - Immunology 2020Quote: ... or WNV MPs (1 µg/mL) or DMSO (0.1%) as negative control together with Brefeldin A (BD GolgiPlug, BD Biosciences) for 6 hours ...
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bioRxiv - Immunology 2020Quote: ... or WNV MPs (1 µg/mL) or DMSO (0.1%) as negative control together with Brefeldin A (BD GolgiPlug, BD Biosciences) for 6 hours ...
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bioRxiv - Neuroscience 2019Quote: ... Nuclei enriched in Nuclei Suspension Buffer were stained with DAPI (1:1000) for nuclei isolation using the Influx cell sorter (BD Biosciences ...
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bioRxiv - Immunology 2019Quote: ... and incubated for 30 min at 4°C with antibodies specific for the following markers: CD45.2-PE-Cy7 (1:200 dilution; BD Biosciences); EpCAM-APC (1:100 dilution ...
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bioRxiv - Cell Biology 2019Quote: Flow cytometry of anti-p-ERK-stained CRC cells resuspended in PBS/1%BSA cells was done using an Accuri cytometer (BD). Cells were gated for populations displaying different tdTomato fluorescence values (negative ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were incubated for 1 h at RT with primary antibody used as follows: mouse anti-EEA1 (BD Biosciences: 610457) 1 µg/mL ...
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bioRxiv - Immunology 2019Quote: ... FCRL6+/FCRL6- pro B (AA4.1+Lin-CD43+B220+CD19-IgM-) cells from the FL and BM were sorted using a FACSAria II sorter (BD).
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bioRxiv - Immunology 2019Quote: ... Single suspensions of FL cells were prepared by crushing the tissue with a 1 ml syringe plunger and passage through a 40 μm strainer (BD). Single cell suspensions were treated with ACK lysing buffer and re-suspended with PBS containing 2% FCS ...
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bioRxiv - Immunology 2019Quote: ... Cell surface markers were immunolabeled with anti-Gr-1 (PE Rat Anti-Mouse Ly6G and Ly6C, BD Pharmingen, Franklin, NJ), F4/80 (Anti-F4/80 PE ...
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bioRxiv - Cancer Biology 2019Quote: ... Tumor xenografts were established in 6-week-old NOD SCID mice via subcutaneous injection into the dorsal region of each mouse with 1 x 107 cells/0.15 ml Hank’s balanced salt solution containing Matrigel® (BD Biosciences). Experiments were performed at five mice in each group (vector control ...
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bioRxiv - Cell Biology 2020Quote: ... at 3:100 or phospho-AKT (pS473) at 1:20 dilutions for 30 mins at RT before analysis on FACSAria II (BD).
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bioRxiv - Cancer Biology 2019Quote: ... the apoptosis of PANC-1 cells was determined by using the Annexin V-FITC/PI apoptosis detection kit (BD Biosciences). The cells were harvested with trypsin-EDTA (Gibco ...
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bioRxiv - Neuroscience 2020Quote: ... and microglia (CD45med CD11b+were sorted into collection tubes coated with FCS and filled with complete RPMI 1640 medium (1% penicillin, streptomycin, 0.1% 2-ME) with 20% FCS using a FACSAria device (BD Biosciences). Purity of sorted populations was routinely above 95% ...
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bioRxiv - Developmental Biology 2021Quote: ... Phenotyping BCAMhi and BCAMlo organoids: Single cells were incubated with anti-human BCAM BV421 (clone B64; 1:50; BD Biosciences), EGFR BV605 ...