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Citations for Becton, Dickinson and Company :
6451 - 6500 of 6970 citations for Cow Caprin 1 CAPRIN1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... The EGFP+stop cassette was inserted in-frame into exon 1 at the AgeI site following amplification from pEGFP-C1 (BD Biosciences) using primers 2-For and 2-Rev ...
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bioRxiv - Immunology 2021Quote: ... GFP-positive transfected cells were sorted in 96-well plates at a density of 1 cell per well using a FACS ARIA III (BD Biosciences) and cultivated in THP-1 media ...
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bioRxiv - Immunology 2021Quote: ... Cells were aliquoted to a count of 1 million cells each and incubated on ice with Fc Block (BD, Table S1) at a 1:20 dilution ...
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bioRxiv - Immunology 2020Quote: ... 8.0 × 105 cells per condition were fixed in 1% PFA for 10 min at room temperature and subsequently stained with primary antibodies: mouse anti-human PEcy7-CD16 (BD #557744), mouse anti-human PerCPcy5.5-CD66b (Biolegend # 305107) ...
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bioRxiv - Genomics 2021Quote: ... the suspension was sorted by applying a 4-way purity mode based on the selected DAPI positive nuclei population (1:1000, BD Pharmingen) using forward and side scatter gating strategy (FACS) ...
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bioRxiv - Plant Biology 2021Quote: ... Samples were run for 5 min at low flow rate (12 ± 3 μl min−1) and cytometric data were documented using BD CellQuest Pro software (BD Biosciences). In addition to glaucophyte cells ...
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bioRxiv - Systems Biology 2020Quote: ... cells were extensively washed with PBS and stained with NeutrAvidin Protein DyLight 488 1:50 for 20 min prior analysis using an Accuri C6 Flow Cytometer (BD Biosciences) with side-scatter (height ...
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bioRxiv - Microbiology 2020Quote: ... during 15 min at 4 °C in MACS buffer followed by incubation with anti-CD45 1:100 (clone 30F-11, BD biosciences), anti-EpCAM 1:100 (clone G8.8 ...
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bioRxiv - Microbiology 2020Quote: ... http://www.eucast.org) and ISO 20776-1:2006 guidelines with the exception that cation-adjusted Mueller-Hinton broth (BD, New Jersey, USA) was supplemented with 100 U of catalase (Worthington Biochemical Corporation ...
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bioRxiv - Cancer Biology 2020Quote: ... For both ACHN and CAKI-1 cell lines 2×106 cells were diluted in 50 µl of PBS and 50 µl of Matrigel (BD Biosciences) and were injected subcutaneously into the right and left flank of each mouse ...
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bioRxiv - Cancer Biology 2020Quote: ... into a 50 mL conical vial using a 10 mL serological pipette and the plunger from a 1 mL syringe (BD, 309628). Cell strainers were washed with 25 mL sterile PBS into corresponding conical vials ...
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bioRxiv - Cancer Biology 2021Quote: ... A primary antibody dilution was prepared in the blocking solution with the antibodies diluted 1:200 for HIF1α (BD Bioscience, 610958), 1:50 for phosphor-S6 (CST ...
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bioRxiv - Cell Biology 2021Quote: ... Crypts were mixed with Col6a1cre-GFP+ and GFP− IMCs sorted by FACS at passage 1 and subsequently resuspended in Matrigel (BD Biosciences) at 250 crypts/50.000 IMCs/30 μl in 48-well plates ...
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bioRxiv - Cancer Biology 2021Quote: ... Flow cytometry for negative checkpoint regulator was performed as previously described(14) using antibodies: BV510 mouse anti-human CD279 (PD1) (1:100; 563076, BD Horizon), PerCP-eFluor 710 mouse anti-human CD223 (LAG-3 ...
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bioRxiv - Cancer Biology 2021Quote: ... The levels of DNA-loaded MCM were detected by incubating cells in anti-MCM2 BM28 antibody (1:200, BD biosciences, 610700) for 1 hr at 37°C in the dark followed by incubation in anti-mouse-488 secondary antibody (1:1000 ...
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bioRxiv - Genomics 2020Quote: ... 3 times 5 min washes with TNT were followed by a 1 h incubation with biotinylated goat anti-rat (559286, BD biosciences) 1:100 in TNB ...
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bioRxiv - Cancer Biology 2022Quote: ... The cells were activated in plates coated with anti-mouse CD3ε and anti-mouse CD28 antibodies (1 µg/ml, BD Biosciences) for up to 72h.
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bioRxiv - Immunology 2022Quote: ... into FACS buffer (1X PBS with 2% FBS and 1 mM EDTA) and analyzed with a FACS Aria flow cytometer (BD Biosciences).
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bioRxiv - Genomics 2022Quote: ... 1 μg of total RNA from NIH/3T3 cells was diluted with 500 μL of BD Rhapsody Lysis buffer (BD Biosciences) supplemented with 15 mM DTT (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... For measurements of CXCL13 the cells were cultured for 5 hours at 37°C in RPMI supplemented with 10% human AB serum and GolgiStop (1/1500; BD Biosciences). For cytokine measurements the cells were plated in the presence of anti-CD3/CD28 (Dynabeads® Human T-activator CD3/CD28 ...
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bioRxiv - Immunology 2022Quote: ... Cells were pelleted by centrifugation at 600 x g for 6 minutes and fixed in 100 μL per 1×106 cells of PhosFlow Lyse/Fix buffer (BD Bioscience) at 37 °C for 10 minutes ...
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bioRxiv - Immunology 2022Quote: Lymphocytes were isolated from the lungs of VSV-OVA-challenged mice and stimulated ex vivo with OVA peptide 1 mM) in the presence of Golgi-Plug (BD Biosciences) for 5 hours ...
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bioRxiv - Immunology 2022Quote: ... and the remaining cells were incubated for 5-6 hrs at 37°C in the presence of 1 uM of the indicated peptide and brefeldin A (GolgiPlug; BD Pharmingen). Surface staining was done overnight at 4 degrees ...
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bioRxiv - Microbiology 2022Quote: ... cells were fixed in 1% (vol/vol) formaldehyde for 30 min at 4°C and analyzed on an LSRFortessa (BD Biosciences) flow cytometer ...
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bioRxiv - Developmental Biology 2022Quote: ... Data concerning bTg-GFP (endogenous expression) and Nkx2-1 expression were obtained and processed using a LSRFortessa X-20 flow cytometer and FACSDiva software (BD Biosciences), respectively ...
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bioRxiv - Microbiology 2022Quote: ... Live CD45+Ly6G+CD11b+Wasabi+ or Wasabi- cells were sorted by FACS in PBS supplemented with 1% BSA using a FACS ARIA Fusion (BD Biosciences). Single cell RNA-sequencing libraries were prepared according to the manufacturer’s instructions using Chromium Single Cell 3′ Library & Gel Bead Kit v3 (10x Genomics ...
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bioRxiv - Cell Biology 2022Quote: ... Cells expressing desired transgenes were isolated by culturing in growth media supplemented with puromycin (1 μg/mL) or using fluorescence-activated cell sorting (FACS) as appropriate (FACS Aria3, BD Biosciences).
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bioRxiv - Immunology 2020Quote: Tumor cells co-cultured with CAR T cells for 1 to 4 h were fixed in 4% PFA for 10 minutes at 4°C and then washed in PBS and stained with anti-ICAM-1 (REA 266, Miltenyi) or anti-phosphorylated STAT1 pY701 (clone 4a, BD Biosciences) antibodies ...
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bioRxiv - Immunology 2020Quote: ... Flow cytometry experiments were performed using PE-anti-human CD54 (ICAM-1) (REA266, Miltenyi) and APC-anti-human CD20 (clone L27, BD Biosciences). Antibodies for blocking LFA-1 - ICAM-1 interaction were anti-ICAM1 (clone HA58 ...
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bioRxiv - Immunology 2021Quote: ... Fc receptors were blocked with Purified Rat Anti- Mouse CD16/CD32 Mouse BD Fc Block™ (2.4G2, BD Pharmingen, 1:1000). Cell surface antigens were stained in PBS containing 2 % (vol/ vol ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were suspended in 200 µl of 1% FBS and cells were sorted by GFP expression using FACSCVantage SE (BD Biosciences). The cell cycle profiles of GFP-positive and GFP-negative cells were determined by Hoescht flourescence using a FACSCalibur flow cytometer (Becton Dickinson) ...
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bioRxiv - Immunology 2021Quote: ... For each sample 200,000 cells were incubated with suitable concentrations of CD4 Nbs coupled to CF568 in combination with 1:500 dilution of anti-CD3-FITC (BD Biosciences) at 4°C for 30 min ...
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bioRxiv - Immunology 2021Quote: ... or GFP-Nb-Cy5.5 (∼51 nM) in 1% FPS/PBS at 4°C for 20 min and subsequently analyzed on a LSR-II cytometer (BD biosciences).
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bioRxiv - Microbiology 2021Quote: Nectin-1 was detected on the surface of cells by staining them with anti-nectin-1 antibody CK41 conjugated to PE (BD Biosciences). HVEM was detected on the surface of cells using the anti-HVEM polyclonal antibody ...
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bioRxiv - Neuroscience 2020Quote: ... cells were incubated in PGB for 25 min with antibodies against Lewis-X (1:20, LeX/CD15 FITC, MMA; BD Bioscience), CD133-APC (1:50,13A4 ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed in 3ml FACS buffer before being fixed in 1% paraformaldehyde and analyzed within 5 days using a Fortessa Flow Cytometer and BD FACSDiva Software (BD Biosciences). Flow cytometric data were analyzed using FlowJo™ (Treestar ...
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bioRxiv - Immunology 2020Quote: ... 0.5-1×106 events were collected in each case on a BD LSR Fortessa X-50 flow cytometer (BD Immunocytometry Systems). Electronic compensation was performed with antibody capture beads (BD Biosciences) ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were stimulated with 100 ng/ml phorbol 12-myristate 13-acetate (PMA) plus 1 µg/ml ionomycin for 4 h at 37°C in the presence of GolgiPlug/GolgiStop (BD Pharmigen). Cells were stained for surface molecules as described above ...
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bioRxiv - Cancer Biology 2020Quote: ... single CD44+CD39+PD-1+CD8+ T cells as well as other CD44+CD8+ T cells were sorted using an ARIA III Sorter (BD Biosciences).
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bioRxiv - Neuroscience 2021Quote: ... P0-P1 mouse pups were anesthetized in ice and then eyes were pierced at the edge of corneal by 30G × 1/2 needle (BD, 305106) under stereomicroscope ...
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bioRxiv - Immunology 2020Quote: ... followed by staining for 3 days at 37 °C with 1:100 dilutions of BV421-labeled mouse anti-human CD35 clone E11 (BD Biosciences) and Alexa Fluor 488-labeled mouse anti-Ki67 clone B56 (BD Biosciences ...
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bioRxiv - Cancer Biology 2021Quote: ... were plated on the lower wells and MCF7 or HFF (1 × 104 cells) were seeded into the top chamber (24-well insert; BD Bioscience) and then co-cultured for 24 hrs (Fig ...
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bioRxiv - Immunology 2021Quote: ... reactivation of latent HIV-1 was determined by quantifying the percentage of GFP+ cells using the FACS ARIA and analyzed using FlowJo (BD Biosciences) software ...
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bioRxiv - Developmental Biology 2022Quote: ... The single cells from BMP4 withdrawal plus FGF2 stimulated VFG culture were incubated only with 1:100 CD117-APC (BD Biosciences) in a similar condition to that described above ...
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bioRxiv - Immunology 2022Quote: ... The yeast clones were washed three times with cold PBS+1%FBS before analyzed by FACS using Aria II (BD Biosciences). Positive yeast clones were sorted and used for nanobody cloning.
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bioRxiv - Immunology 2022Quote: ... The permeabilized cells were stained with intracellular cytokine staining antibodies for 20 min at room temperature in 1 perm/wash buffer (BD Biosciences). Cells were then washed twice with perm/wash buffer and once with staining buffer before acquisition using the BD Symphony Flow Cytometer and the associated BD FACS Diva software ...
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bioRxiv - Immunology 2022Quote: ... Cells were resuspended in 1% para-formaldehyde until acquisition on a BD Biosciences Symphony cytometer and analyzed using FlowJo (BD Biosciences). Caspase 3 staining was performed using PE Active caspase 3 Apoptosis Kit (BD ...
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bioRxiv - Cancer Biology 2022Quote: ... Immunohistochemistry (IHC) was performed on FFEP blocks using mouse monoclonal antibodies SMARCB1/INI1 (Clone 25/BAF47, dilution 1:200, BD Bioscience) and CD70 (Clone 301731 ...
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bioRxiv - Genomics 2022Quote: ... This was followed by the secondary labeling step with 1:200 dilution of Streptavidin PE-Cy7 antibody (BD Biosciences, Cat#: 557598). Cells were isolated and sorted using an Influx Cell Sorter Instrument (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... Tracheas were stretched out by pinning to SylGard-coated well of 6 well plate and fixed for 1 hr on ice in Cytofix/Cytoperm buffer (BD Biosciences). Immunofluorescent staining was performed in PBS with 0.25% Triton X-100 at 4°C as follows ...