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Citations for Becton, Dickinson and Company :
601 - 650 of 935 citations for Gentamicin Deuterated C Complex Pentaacetate Salt Contains d0 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... 1% bovine serum antigen) and stained with either 1:20 anti-HLA-A,B,C-PE (clone W6/32, BD Biosciences), 1:100 anti-β2m-FITC (clone 2M2 ...
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bioRxiv - Cancer Biology 2021Quote: ... for 30 minutes at 4°C and consecutively sorted using a SONY SH800S or a FACS Aria Cell Sorter (BD Biosciences) into NCAM1- and NCAM+ populations ...
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bioRxiv - Cancer Biology 2021Quote: ... for 30 minutes at 4°C and sorted into 384-wells SORTseq plates using a FACS Aria Cell Sorter (BD Biosciences) and directly stored at −80°C until further processing.
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bioRxiv - Microbiology 2020Quote: ... washed with cold PBS by centrifugation (5 min, 550g at 4°C) and analysed with a FACS BD AccuriTM C6 (BD Biosciences) instrument ...
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bioRxiv - Microbiology 2020Quote: ... cells were stored at 4°C until staining with combinations of fluorochrome-conjugated monoclonal antibodies including: anti-CD3 (SP34-2: Pacific Blue; BD Biosciences), anti-CD4 (L200 ...
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bioRxiv - Immunology 2021Quote: ... for 5 minutes prior to staining at 4°C in the dark using the antibodies listed in supplementary table 1 and analysed using an LSR Fortessa cytometer (BD Biosciences) and FlowJo software (BD) ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were incubated for 30 min in the dark at 4°C after adding relevant antibodies (BD biosciences, Franklin Lakes, NJ). A FACSCalibur system (BD biosciences ...
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bioRxiv - Immunology 2021Quote: ... and surface stained for 30 mins at 4°C with 50 μL/well anti-mouse CD3 APC-CY7 (clone 17A2, 1:400, BD Biosciences), anti-mouse CD4 BV510 (clone RM4-5 ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed twice and incubated overnight at 4°C with the fluorochrome-conjugated antibodies and unconjugated rat and mouse IgG in Perm/Wash solution (BD Biosciences), which was followed by additional 5 min incubation in Perm/Wash solution without antibodies ...
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bioRxiv - Cell Biology 2022Quote: Cells harvested at different time points of reprogramming were stained in FACS buffer for 30 min at 4°C and washed with FACS buffer prior to acquisition with LSR Fortessa (BD Biosciences) cytometer ...
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Genetically engineered microglia-like cells have therapeutic potential for neurodegenerative diseasebioRxiv - Neuroscience 2021Quote: ... and with hematopoietic stem cell markers APC-conjugated anti-CD117 (c-Kit) and PE-conjugated Ly6A/E (Sca-1) (BD Biosciences). Lineage negative cells were transduced with a lentiviral vector in a cell incubator at 2 ×106 cells/mL in either StemSpan SFEM II (STEMCELL Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated overnight at 4°C with the astrocyte (mouse anti-GFAP; 1:1000, BD Biosciences, San Diego, CA, USA) and microglia (rat anti-CD11b ...
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bioRxiv - Molecular Biology 2020Quote: ... High sensitivity C-reactive protein (CRP) was measured in serum: blood was collected into a silica and gel containing tube (BD Vacutainer).
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bioRxiv - Molecular Biology 2020Quote: ... resuspended in cold phosphate-buffered saline (PBS) containing 3% FBS and stored at 4°C until analysis using a LSRII flow cytometer (BD Biosciences). FlowJo software v10 (Treestar ...
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bioRxiv - Cell Biology 2022Quote: ... was directly added to the cells and incubated for 30 mins at 37°C and analyzed using a Fortessa flow cytometer (BD Biosciences) at the Imaging and Flow cytometry Core ...
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bioRxiv - Genomics 2021Quote: ... tissues were blocked (3% bovine serum albumin in 5% normal goat sera and 0.1% Triton) and incubated overnight at 4°C with primary antibodies: CD31 (Neomarkers, RB-10333-P0) or CDH1 (BD Biosciences, 6315829). Slides were washed and incubated with Cy3-goat-anti-mouse or anti-rabbit-conjugated secondary antibodies (Jackson Immunoresearch Laboratories ...
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bioRxiv - Immunology 2021Quote: Cultured T cells were stimulated with the cognate SARS-CoV-2 OPPs (ORF1ab-C, ORF3a, N, S, nsp12) and incubated for 4 hours in the presence of Golgiplug (BD Biosciences). Following stimulation ...
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bioRxiv - Cancer Biology 2020Quote: Tumor cells were implanted subcutaneously in the flank of BALB/c mice using 5 × 106 cells (WEHI-164) with 0.5 ml 29G insulin syringes (MicroFine™+, BD medical). Tumor volume was measured with a caliper and volume was calculated using the formula ...
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bioRxiv - Immunology 2021Quote: ... and stained for 20 minutes at 4°C with 50 μl of antibody cocktail containing subset-specific antibodies (Supplementary Table 2) diluted in Brilliant Stain Buffer (BD Biosciences). Cells were washed in FACS buffer and fixed for 20 minutes at 4°C in 100 μl of Fixation Buffer (BioLegend).
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bioRxiv - Microbiology 2021Quote: ... neoformans strains were grown at 30°C in yeast extract-peptone-dextrose (YPD) agar or liquid medium (BD Biosciences, Sparks, MD). S ...
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bioRxiv - Bioengineering 2020Quote: Cell surface staining was performed at 4 °C for 30 min and was analysed using a FACSCalibur flow cytometer (BD Biosciences) and CellQuest Software (BD Biosciences) ...
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bioRxiv - Bioengineering 2020Quote: Cell-surface staining was performed for 45 min at 4 °C and was analyzed using a FACSCalibur flow cytometer (BD Biosciences). A minimum of 1 × 104 events per sample were examined.
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bioRxiv - Neuroscience 2020Quote: ... The pellet was recovered and sequentially incubated with anti-mouse CD16/CD32 (1:250, BD Pharmingen; for 15min at 4°C) followed by rat anti-CD11b and PerCP-Cy5.5 rat anti-mouse CD45 (1:200 ...
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bioRxiv - Neuroscience 2020Quote: ... followed by rat anti-CD11b and PerCP-Cy5.5 rat anti-mouse CD45 (1:200, BD Pharmingen; for 30min at 4°C), all in 2% BSA ...
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated overnight at 4°C with IFN-γ APC (1:25, Clone XMG1.2, Biolegends) and IL-17 PE (1:50, Clone TC11-18H10, BD Pharmingen) in Perm/Wash buffer ...
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bioRxiv - Neuroscience 2020Quote: ... cells were incubated for 1 hr at 4°C with extracellular domain anti-GluN1 antibody (BD biosciences; 2 μg ml−1) followed by two washes with ice cold phosphate buffered saline (PBS ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were then washed twice 5 min in Tris buffer saline containing 0.01% Triton X-100 (TBST) and incubated with the primary antibody over night at 4°C (human and mouse a-Syn: 610786 BD Biosciences). After incubation with the first antibody ...
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bioRxiv - Cancer Biology 2020Quote: ... Discs were then washed three times for 10 minutes each in 0.1% PBST and incubated overnight at 4°C in mouse anti-BrdU primary antibody (B44) (BD, 1:50). The next day ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were again pelleted at 500xg for 5 min at 4°C then resuspended in Cytofix/Cytoperm reagent (BD Biosciences) and incubated on ice for 30 min Following fixation/permeabilization ...
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bioRxiv - Cell Biology 2021Quote: ... Coverslips were incubated for 25 mins at 37°C 5% CO2 and then fixed and permeabilized with CytoFix/CytoPerm (BD Biosciences) at room temperature for 20 mins ...
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bioRxiv - Microbiology 2020Quote: ... lymphocytes were cultured in 96-well dishes at 37°C for 5-6 h in the presence of 2 μM peptide pool and brefeldin A (BD Biosciences). Cells were then labeled for cell-surface markers ...
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bioRxiv - Microbiology 2021Quote: ... Isolates were resuscitated from cryo stocks preserved at - 80°C by streaking a loopful of each cryo stock onto brain heart infusion (BHI) agar (BD Difco) and incubating a streaked plate for 15-18 h at 32ºC ...
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bioRxiv - Microbiology 2020Quote: Candida strains (Supplementary Table S1) were routinely grown at 30°C in yeast peptone dextrose (YPD) media (2% peptone (BD Bacto), 2% D-glucose (Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... during 15 min at 4 °C in MACS buffer followed by incubation with anti-CD45 1:100 (clone 30F-11, BD biosciences), anti-EpCAM 1:100 (clone G8.8 ...
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bioRxiv - Immunology 2022Quote: Shigella flexneri 2a WT strain 2457T and avirulent plasmid-curated strain BS103 were grown from frozen stocks (−80°C) on Tryptic Soy Agar (TSA) (Difco BD, USA) supplemented with 0.01% Congo Red dye (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... For measurements of CXCL13 the cells were cultured for 5 hours at 37°C in RPMI supplemented with 10% human AB serum and GolgiStop (1/1500; BD Biosciences). For cytokine measurements the cells were plated in the presence of anti-CD3/CD28 (Dynabeads® Human T-activator CD3/CD28 ...
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bioRxiv - Immunology 2022Quote: ... overlapping peptides (10µg/mL) or media alone for 60 hours at 37 °C and 5% CO2 with proliferation dye (Violet Proliferation Dye, BD Biosciences). Peptides were generated in 15mer format with 8 amino acid overlaps for the ID91 fusion antigen ...
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bioRxiv - Immunology 2022Quote: ... and the remaining cells were incubated for 5-6 hrs at 37°C in the presence of 1 uM of the indicated peptide and brefeldin A (GolgiPlug; BD Pharmingen). Surface staining was done overnight at 4 degrees ...
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bioRxiv - Microbiology 2022Quote: ... cells were fixed in 1% (vol/vol) formaldehyde for 30 min at 4°C and analyzed on an LSRFortessa (BD Biosciences) flow cytometer ...
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bioRxiv - Physiology 2022Quote: ... Cell suspensions were washed and re-suspended in PBS at 4°C and analysed using a BD 5L LSR Fortessa and BD FACSDiva software (BD Biosciences). To exclude dead cells ...
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bioRxiv - Neuroscience 2021Quote: ... and their Fc receptors were blocked for 10 min at 4 °C with anti-CD16/CD32 antibodies (10 μg/mL: BD Biosciences). After blocking ...
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bioRxiv - Biochemistry 2020Quote: ... followed by incubation with fluorochrome-conjugated antibodies for 30min at 4°C in the dark in brilliant stain buffer (BD Biosciences). Prior to fixation and permeabilization for intracellular staining with Cytofix/Cytoperm (BD Bioscience) ...
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bioRxiv - Immunology 2020Quote: ... The cells were incubated for one hour at 4°C with a cocktail of fluorochrome-labelled antibodies consisting of anti-CD3-BV510 (cat. no: 563024, BD Biosciences), anti-B220-APC-Cy7 (cat ...
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bioRxiv - Immunology 2021Quote: ... or GFP-Nb-Cy5.5 (∼51 nM) in 1% FPS/PBS at 4°C for 20 min and subsequently analyzed on a LSR-II cytometer (BD biosciences).
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bioRxiv - Cancer Biology 2020Quote: ... The membrane was blocked with 5% non-fat dry milk (Marvel) and subsequently incubated (O/N, 4°C) with primary antibodies HIF-1α (BD Biosciences) Lamin A (Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were stimulated with 100 ng/ml phorbol 12-myristate 13-acetate (PMA) plus 1 µg/ml ionomycin for 4 h at 37°C in the presence of GolgiPlug/GolgiStop (BD Pharmigen). Cells were stained for surface molecules as described above ...
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bioRxiv - Immunology 2020Quote: ... followed by staining for 3 days at 37 °C with 1:100 dilutions of BV421-labeled mouse anti-human CD35 clone E11 (BD Biosciences) and Alexa Fluor 488-labeled mouse anti-Ki67 clone B56 (BD Biosciences ...
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bioRxiv - Microbiology 2021Quote: Spleen and liver cells were incubated (20 min, 4°C) with anti-Fc-gamma blocking antibody (clone 2.4G2) (BD Biosciences, Belgium) and were subsequently stained with fluorescent conjugated antibodies (30 min ...
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bioRxiv - Immunology 2021Quote: ... Spleen cells of immunized mice were incubated with 50 μM of each peptide for 5 hours at 37°C in the presence of brefeldin A (GolgiPlug, BD Biosciences), and were stained with FITC-conjugated anti-mouse CD8 mAb (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... spleen cells of immunized mice were incubated with 50 μM of each peptide for 6 hours at 37°C in the presence of monensin (GolgiStop, BD Biosciences) and 0.8 μg of FITC-conjugated anti-mouse CD107a mAb (BioLegend) ...