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Citations for Becton, Dickinson and Company :
601 - 650 of 873 citations for Annexin A10 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Adherent and non-adherent cells in the medium were collected and stained with Annexin-FITC and propidium iodide for 15 min (BD biosciences 556547) as per the manufacturer protocol ...
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies for immunoblot were as follows: AURKA (1:1000; mouse mAb clone 4/IAK1, BD Transduction Laboratories), phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C [1:1000 ...
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bioRxiv - Immunology 2020Quote: ... and stained with p-(S465/S467)-Smad2/p- (S423/S425)/Smad3-Alexa 647 mAb Phosflow antibody (BD Biosciences) for 30 minutes at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... Primary antibodies for immunoblot were as follows: AURKA mouse mAb (1:1000; Clone 4/IAK1, BD Transduction Laboratories), phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (1:1000 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were stained with PE-conjugated anti-human CD33 or isotype control mAbs (BD Pharrmingen, San Jose, CA).
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bioRxiv - Cell Biology 2021Quote: Primary antibodies for immunoblot were as follows: AURKA mouse mAb (1:1000; Clone 4/IAK1, BD Transduction Laboratories), phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (1:1000 ...
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bioRxiv - Microbiology 2019Quote: ... Receptors FcγIII (CD16) and FcγII (CD32) were blocked by adding 20 µl of 2.4G2 mAb (BD Pharmigen N553142). Cells were then incubated with 10 μg/ml of biotinylated HCV E2mc3 protein ...
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bioRxiv - Molecular Biology 2019Quote: ... Chemerin was detected using biotin-conjugated rat-anti mouse chemerin mAb (BAM2325) followed by streptavidin-HRP (BD Science). The reaction was developed with TMB substrate (BD Science) ...
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bioRxiv - Immunology 2021Quote: ... The following mAbs were used to label cell surface markers: anti-CD3 PerCP-Cy5.5 (BD, clone 145-2C11), anti-CD4 Alexa Fluor 700 (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... The binding of mAbs to spikes expressing on cell surface was analyzed using flow cytometry (BD Canto Analyzer). Percent (% ...
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bioRxiv - Immunology 2020Quote: ... and stained with fluorescein isothiocyanate (FITC)-conjugated mAb against mouse kappa light chains (BD Biosciences, San Diego, CA) and/or phycoerythrin (PE)-conjugated mAb against mouse CD9 ...
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bioRxiv - Immunology 2021Quote: ... and saturating concentrations of phenotyping mAbs (Table S2) diluted in 50% 1xPBS 50% Brilliant Violet Buffer (BD Biosciences). For identification of SARS-CoV-2 antigen specific B cells 1ug per 500ul of stain each of tetrameric Spike-APC and Spike-PE and 0.5ug per 500ul stain of tetrameric RBD-BV421 were added to the cell preparation ...
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bioRxiv - Immunology 2024Quote: ... Cells stained with anti-CD177 mAb or isotype control were analyzed on a FACSCelesta flow cytometer (BD Biosciences). The FlowJo software (Tree Star Inc. ...
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bioRxiv - Immunology 2023Quote: ... except that mAbs to pSTAT5 and GATA-3 were prepared in 1× BD Perm/Wash buffer (BD Biosciences).
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bioRxiv - Biophysics 2022Quote: ... The binding of mAbs to spikes expressing on cell surface was analyzed using flow cytometry (BD Canto Analyzer). Percent (% ...
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bioRxiv - Immunology 2023Quote: ... and incubated on ice for 30 minutes with fluorochrome-labeled mAbs directed to interferon gamma (clone B27, BD), IL-17 (clone eBio64DEC17) ...
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bioRxiv - Immunology 2024Quote: ... 2mM EDTA) and incubated with Mouse BD Fc Block purified anti-mouse CD16/CD32 mAb 2.4G2 (BD Biosciences) to block non-specific staining ...
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bioRxiv - Cancer Biology 2021Quote: ... The analysis was repeated three times according to the manufacturer’s instructions of Annexin V-FITC/PI kit (BD ISRI Sorp, Franklin Lakes, USA).
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bioRxiv - Biochemistry 2019Quote: ... Annexin V binding was measured using the BD FACSCalibur platform and analyzed using the BD CellQuest Pro software (BD Biosciences, San Jose, CA), 10,000 cells from each sample were analyzed and each sample performed in biological triplicate.
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bioRxiv - Biochemistry 2021Quote: ... Cells were incubated in the dark at RT with 3 μL propidium iodide (Miltenyi Biotech; cat. 130-093-233) and 3 μL FITC-Annexin V (BD Biosciences; cat. 556419) for 20 minutes and then diluted with 100 μL binding buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were stained with 10 μg/ml propidium iodide (PI) staining solution from FITC Annexin V Apoptosis Detection Kit (BD Pharmingen; Cat#: 556547) and 0.2 mg/mL RNase A (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... The apoptotic rates were analyzed by the Annexin V-FITC and propidium iodide (PI) double detection kit (BD Biosciences, San Jose, CA, USA), and the rinsed cells were resuspended in binding buffer containing Annexin V-FITC and PI and then incubated at RT for 15 min in the dark ...
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bioRxiv - Cancer Biology 2019Quote: ... For apoptosis analysis was performed by a double staining Annexin V and 7-AAD Apoptosis Detection Kit (BD Pharmingen, San Jose, CA, USA) following the manufacture’s introductions ...
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bioRxiv - Genomics 2019Quote: ... fluorescein isothiocyanate (FITC)- mouse anti-human Annexin-V (biolegend; Cat# 640945) and APC-anti-human anti-CD42b (GPIb) monoclonal antibody (BD Pharmingen; Cat# 551061). The calcein AM blue was used to identify live platelet sized events ...
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bioRxiv - Cancer Biology 2022Quote: The apoptotic rate was determined using Hoechst 33342 staining following fluorescence-microscopic evaluation as well as by annexin V– FITC staining kit (BD Pharmingen, CA, USA) following the manufacturer’s instructions.HCT-116、HCT-116 Bax−/− cells (1×105 cells/well ...
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bioRxiv - Cell Biology 2023Quote: ABT-737-treated or SARS-CoV-2-infected cells (detached using Trypsin/EGTA) and their respective conditioned media were combined and stained with fluorescently-labelled annexin A5 (BD Biosciences, 1:2,000) and 0.2 μM TO-PRO-3 (Life Technologies ...
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bioRxiv - Cancer Biology 2020Quote: Cells were treated as indicated and stained with fluorescent conjugates of annexin-V and PI (1 µg/ml final concentration) and analyzed on a FACSCanto machine (BD, Franklin Lakes, NJ, USA). Annexin V was prepared as previously described (Brumatti et al. ...
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bioRxiv - Cancer Biology 2022Quote: Cell viability was determined using a resazurin assay and cell death measured using the Annexin-V FITC apoptosis detection kit (BD Biosciences, San Jose, CA). Methods have been previously described (65 ...
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bioRxiv - Cell Biology 2022Quote: ... and their viability was tested using the annexin-PI apoptosis detection kit (MBL, USA) followed by flow cytometry analysis (BD Biosciences FACS Canto II).
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bioRxiv - Molecular Biology 2022Quote: ... cells were washed with ice-cold DPBS plus 5 mM EDTA and stained with annexin V and PI (BD Biosciences, Franklin Lakes, NJ, USA) for 15 min at RT ...
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bioRxiv - Genomics 2024Quote: ... the CD34+ cells were carefully pipetted out and their viability was measured using Annexin V apoptosis assay kit (BD Pharmingen, San Diego, CA, USA) as per the manufacturer’s protocol and counter stained with DAPI.
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bioRxiv - Immunology 2022Quote: ... CD45+ skin cells or expanded MAIT cells were activated (TCR dynabeads) for 18 hours before determining RORγt expression using a specific RORγt mAb (BD Biosciences) and transcription factor staining kit (Biolegend) ...
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bioRxiv - Microbiology 2019Quote: ... 0.02% NaN3 in PBS) and stained with a FITC-conjugated anti-human HLA-DR mAb (Clone Tü36; BD Biosciences). This antibody specifically binds to a monomorphic epitope of the HLA-DR αβ complex and not the isolated α or β chains or the HLA-DP and -DQ isotypes41 ...
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bioRxiv - Immunology 2021Quote: ... The following monoclonal antibodies (mAbs) were used in this study: anti-BrdU-FITC (3D4, BD Biosciences, San Jose, CA), CD45-PE (MB4-6D6 ...
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bioRxiv - Immunology 2021Quote: ... Cells were then washed twice in PBS and stained with mAbs to CD69 (APC, FN50, BD Biosciences USA, #555533) and CD3 (APC-Cy7 ...
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bioRxiv - Immunology 2020Quote: PBMCs (1−2×106) were stimulated with epitope-specific peptides (5 μM) and anti-CD28 mAb (0.5 μg/mL, BD) and expanded for 14 days in complete RPMI culture medium containing rIL2 (20 IU/mL ...
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bioRxiv - Cell Biology 2024Quote: ... or magnetic beads coupled to an anti-BAK antibody able to recognize the Nt region of BAK only when activated(49) (mouse mAb, clone G317.2, BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... Twenty 8-11 months-old Gata1low mice were randomly divided in two groups which were treated either with vehicle (2% v/v dimethyl sulfoxide in H2O) or with biotinylated mAb RB40.34 (BD Pharmigen ...
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bioRxiv - Microbiology 2022Quote: ... PE-Cy7-anti-CD4 (clone RM4-5) and BV711-anti-CD8 (clone 53-6.7) mAbs (BD Biosciences or eBioscience). Cells were washed in FACS buffer ...
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bioRxiv - Immunology 2023Quote: ... 2×106 cells/well (6-well plate) were cultured with 1μg/mL of anti-CD3 mAb (553057, BD Biosciences) plus 10 ng/mL of mouse IL-2 (Z02764 ...
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Treg cells drive MYCN-mediated immunosuppression and tumor aggressiveness in high-risk neuroblastomabioRxiv - Cancer Biology 2023Quote: ... and then stained with Alexa Fluor 647 conjugated anti-FOXP3 mAbs (1:100, Clone 259D/C7, BD Biosciences, 560044) to identify CD4+CD25+FOXP3+ Treg cells ...
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bioRxiv - Immunology 2021Quote: ... or for 4 h with 20 ng/ml recombinant mouse IL-4 (BD Pharmigen). Cells were then washed twice with isotonic buffer composed of 147 mM NaCl ...
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bioRxiv - Biophysics 2020Quote: ... and 5 to 45 µl of suspension was added to 300 µl of annexin V binding buffer (Becton Dickinson, BD Bioscience, San Jose, CA, USA). After the addition of 2.5 µl of annexin V-FITC (Becton Dickinson ...
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bioRxiv - Cancer Biology 2020Quote: ... The viability of the cells was measured post 48 hours of drug treatment using annexin-V-7AAD viability kit (BD Pharmingen, San Diego, CA, USA) as per the manufacturer’s protocol.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and resuspended in a binding buffer containing 1 μg/mL propidium iodide (PI) and 1 μg/mL APC-labeled annexin V (BD Pharmingen, San Diego, CA, USA). All specimens were analyzed by flow cytometry (FACSCalibur ...
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bioRxiv - Cell Biology 2023Quote: ... Stained cells were diluted 4x in 1x Annexin V Binding Buffer and analysed by flow cytometry performed using the BD FACSCanto II (Becton Dickinnson and Company, BD Biosciences, San Jose, CA, USA). Data was analysed using FlowJoTM Software ...
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bioRxiv - Immunology 2021Quote: ... by negative selection with single-cell splenocyte suspensions by using biotinylated anti-Thy1.2 mAb followed by streptavidin-conjugated microbeads (BD Pharmingen). In brief ...
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bioRxiv - Immunology 2021Quote: ... and pre-incubated with 0.5 μg/ml FcBlock (eBioscience) and stained with fluorochrome-conjugated mAbs (Biolegend, eBioscience, and BD Biosciences): CD19 (1D3) ...
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bioRxiv - Immunology 2022Quote: ... cells were first stained for surface markers and then fixed and permeabilized using Intracellular Fixation and Permeabilization Buffer Set (eBioscience) before incubation with fluorochrome-conjugated mAbs against mouse IFNγ (clone XMG1.2, BD Bioscience). Flow cytometry was performed on LSRFortessa (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... Vα7.2+ cells were pre-incubated for 1 h and 30 min with LukED at 0.312 μg/mL before adding to the bacteria-pulsed THP-1 and co-culture at 2:1 ratio for 24 h in the presence of 1.25 μg/mL anti-CD28 mAb (L293, BD Biosciences). Monensin and brefeldin A (both from BD Biosciences ...