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Citations for Becton, Dickinson and Company :
601 - 650 of 3553 citations for 2' 5' Dichloro 3 3 4 dimethylphenyl propiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Intracellular staining was carried out with the following antibodies diluted in BD Perm/Wash buffer: Active-caspase-3-GranzymeB-PE (C92-605, BD, cat #561011), Active-caspase-3-BV650 (C92-605 ...
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bioRxiv - Immunology 2024Quote: ... cells were suspended in 0.5 mL PBS+3% FBS and stained for flow cytometry analysis by adding anti-CD16/CD32 antibody (BD Pharmingen; clone 2.4G2) ,anti- CD117 (Biolegend clone 2B8) ...
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bioRxiv - Bioengineering 2024Quote: The expression of the exhaustion markers PD-1 and TIM-3 was evaluated using anti-PD1 (cat. 560795, BD Biosciences, BD PharMingen) and anti-TIM3 (cat ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue was weighed then homogenized for 3 minutes in a bullet blender and serum was added to a serum separator tube (BD Microtainer: 365967) before being spun down and diluted 1:200 ...
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bioRxiv - Microbiology 2024Quote: ... Unspecific binding of antibodies was blocked by incubating the samples with 3% rat serum in BD staining buffer (BD Biosciences, Heidelberg, Germany) for 5 minutes at RT ...
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bioRxiv - Cancer Biology 2024Quote: ... and VC3ai cleavage by caspase-3/7 was assessed based on fluorescence emission at 530 nm using a BD LSR Fortessa™ Cell Analyzer (BD Biosciences).
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bioRxiv - Microbiology 2021Quote: Streptococcus pneumoniae TIGR4 (serotype 4) was cultured statically in Todd Hewitt broth with 5% yeast extract (THY, BD Biosciences) supplemented with 300 U/ml catalase (Worthington Biochemicals ...
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bioRxiv - Immunology 2020Quote: The following antibodies and reagents were used for flow cytometry: CD4 clone RM-4-5 (BD Biosciences, BioLegend, Tonbo), CD8α clone53-6.7 (BD ...
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bioRxiv - Genetics 2024Quote: ... Following blocking (5% milk/BSA) membranes were incubated with primary antibody overnight at 4°C (BIP [1:40,000 BD Transduction 610979] ...
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bioRxiv - Cell Biology 2023Quote: The viability of the cultured cells was determined by DAPI (4’,6-Diamidino-2-Phenlindole, Dihydrochloride; BD Pharmingen), a nucleic acid stain that is excluded from viable cells ...
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bioRxiv - Cell Biology 2021Quote: ... by subcutaneous injection of 3 × 105 A375 or H2122 cells in RPMI-1640 medium supplemented with 50% Matrigel (BD Biosciences, cat. no. 354234). Seven days after the injection ...
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bioRxiv - Immunology 2023Quote: ... Mice were inoculated subcutaneously (50uL) with 3×104 PFU or injected intracranially (10uL) with 50 PFU of LGTV-TP21 using insulin syringes (BD Medical, #BD-329461). At appropriate times post infection ...
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bioRxiv - Pathology 2023Quote: ... The culture medium was sampled by adding 1-3 drops to microscope slides followed by Gram stain (BD BBLTM Gram Stain Kits; B12539) and imaging.
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bioRxiv - Neuroscience 2022Quote: ... Following overnight incubation the plate was washed 3 times with DPBS and then incubated with 20 µl anti-CD45 antibody (BD Pharmingen, catalog #550539) in 12 ml of 0.2% BSA for a minimum of 2 hours ...
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bioRxiv - Microbiology 2023Quote: ... and allowed to gel over a 1-mL layer of acellular collagen in six-well culture inserts with 3-μm-pore polycarbonate filters (BD Biosciences NJ, USA). Gels were allowed to contract for 4–5 days before seeding with 3×105 HaCaT keratinocytes in serum and mitogen-supplemented DMEM/F12 raft medium ...
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bioRxiv - Immunology 2024Quote: ... n=3 samples per genotype) for FACS enrichment of islet immune cells via BD FACS ARIA II (BD Biosciences, San Jose, CA, USA) into PBS containing 2% FBS (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... All experiments were performed with n ≥ 3 from independent overnight cultures starting from single colonies on LB agar plates (BD; Franklin Lakes, NJ).
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bioRxiv - Neuroscience 2023Quote: ... The cell suspension was then centrifuged at 300 × g for 5 min at 4 °C and cells resuspended in 100 µL of stain buffer (BD Biosciences ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... for 5 min at 4 °C and immunostained with CD11b-AlexaFluor 647 (M1/70; 1:1000; Cat#557686, BD Biosciences) and CD45-PE (1:1000 ...
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bioRxiv - Developmental Biology 2022Quote: PI-annexin V apoptosis assay was performed at differentiation week 2 and week 4 in normal and HGPS iPSCs-keratinocytes induction according to the manufacturer’s instruction (BD). In brief ...
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bioRxiv - Developmental Biology 2021Quote: ... the E13.5 dams were injected intraperitoneally with 200 μl of 5-bromo-2’-deoxyuridine (BrDU) (552598, BD Pharmingen) early in the morning and the embryonic kidneys were collected 2.5 hours later ...
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bioRxiv - Genomics 2021Quote: ... supplemented with 10% modified ADC (2% glucose, 5% BSA, 0·85% NaCl) or in Middlebrook 7H9broth (BD Biosciences) supplemented with 10% modified ADC along with 0.05% Tween 80 without shaking.
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bioRxiv - Immunology 2021Quote: ... then washed and resuspended in PBS-2% FBS for flow acquisition on a 5-laser LSRII (BD BioSciences). For experiments with intranuclear transcription factor staining ...
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bioRxiv - Developmental Biology 2023Quote: 5-ethynyl-2’-deoxyuridine (EdU) staining was performed using the 647 EdU Click Proliferation Kit (BD Biosciences, 565456). First ...
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bioRxiv - Physiology 2021Quote: ... Sections were then washed with PBS 3 times for 15 min and incubated with PBS containing Hoechst (1:5000, #561908, BD Pharmingen, Lincoln Park, NJ) for 5 min before a final series of washes with PBS.
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bioRxiv - Bioengineering 2023Quote: ... so that the microfluidic tubing (Scientific Commodities,Inc., BB31695-PE/3) can connect the device to the sample sources in a syringe (BD 1 mL, NJ, USA).
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bioRxiv - Cancer Biology 2024Quote: ... The phosphorylation-SMAD2/3 ability of CAR T cells was detected using PE Mouse anti-Smad2/Smad3 antibodies (clone O72-670, BD Biosciences, Franklin Lakes, NJ) following the staining protocols ...
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bioRxiv - Immunology 2022Quote: ... and F15 NOD Nfκb1 heterozygote were suspended in biotin-free RPMI containing 0.1% azide and 3% FCS and surface stained in 96-well plates with the 10–3.6 PE (anti-I-Ag7) (BD PharMingen, Franklin Lakes, NJ, USA), which is class II major histocompatibility complex (MHC ...
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Angiogenesis in the mature mouse cortex is governed in a region specific and Notch1 dependent mannerbioRxiv - Neuroscience 2024Quote: ... sections were incubated overnight in 0.1M PBS + 0.1% TX-100 solution containing primary antibody for rat anti-CD31 (label endothelial cells; 1:200 dilution, BD Clone MEC 13:3: #553370) or sheep anti-CD93 (label endothelial tip cells ...
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bioRxiv - Pathology 2024Quote: ... phosphorylated TDP-43 (clone 11-9, 1:2000, CosmoBio, Tokyo, Japan) and anti-p62 (3/P62 LCK LIGAND, 1:500 BD Company, NJ, United States) were employed ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... analysis of sub-G0/G1 DNA content as previously described (23) and by measurement of active caspase-3 by flow cytometry using a commercial kit (BD Biosciences, Franklin Lakes, NJ).
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bioRxiv - Microbiology 2020Quote: ... washed with cold PBS by centrifugation (5 min, 550g at 4°C) and analysed with a FACS BD AccuriTM C6 (BD Biosciences) instrument ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Membranes were incubated with primary antibodies overnight at 4℃ after 1h blocking in 5% skimmed milk (Becton, Dickinson and Company, USA) at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were again pelleted at 500xg for 5 min at 4°C then resuspended in Cytofix/Cytoperm reagent (BD Biosciences) and incubated on ice for 30 min Following fixation/permeabilization ...
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bioRxiv - Cancer Biology 2020Quote: ... The membrane was blocked with 5% non-fat dry milk (Marvel) and subsequently incubated (O/N, 4°C) with primary antibodies HIF-1α (BD Biosciences) Lamin A (Sigma ...
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bioRxiv - Bioengineering 2023Quote: ... was grown overnight (37°C, 4% H2, 5% CO2, 91% N2, <10 ppm O2) in Chopped Meat Carbohydrate Broth (BD297307, BD, USA). Genomic DNA was extracted from B ...
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bioRxiv - Immunology 2023Quote: ... the cells were stained with the corresponding FACS panels (Extended Data Table 5) at 4 °C for 30 minutes and sorted using BD Aria III (BD Biosciences) or MoFlo Astrios EQ (Beckman Coulter ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell suspensions were blocked for 5 min at 4 °C with rat anti-mouse CD16/CD32 (#553142, Mouse BD Fc Block, BD Biosciences) in FACS buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... the pLCOs in suspension were centrifuged at 500 × g for 5 minutes at 4 °C and then resuspended in cold growth factor-reduced Matrigel (BD Biosciences). Subsequently ...
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bioRxiv - Immunology 2023Quote: ... Effector cells were incubated with K562 and Nalm6 variants for 4 hours at a ratio of 5:1 with addition of Brefeldin A (GolgiPlug, BD Biosciences). Next ...
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bioRxiv - Genomics 2020Quote: ... each anaesthetised snail was injected with 170 μL of snail saline (see Standen 1975) in the head-foot using a syringe and needle (0.45 mm × 16 mm BD Microlane™ 3, New Jersey, USA). This treatment examined the effect of wounding in activating immune defence when compared to anaesthesia without exposure to immune elicitors (treatment 2 above) ...
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Importin α/β Promote Kif18B Microtubule Association to Spatially Control Microtubule DestabilizationbioRxiv - Cell Biology 2022Quote: ... Reactions were fixed with 40 µL of 1% glutaraldehyde/BD buffer for 3 min and diluted with 800 µL BRB80 (BD buffer without 1 mM DTT). Fixed and diluted samples were added to spin down tubes containing a removable chock ...
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bioRxiv - Microbiology 2023Quote: ... Prokaryotic cells and virus-like particles (VLPs) from site #1 – #3 were measured on a flow cytometer (BD Accuri C6, BD Biosciences, Franklin Lakes, NJ, USA) by using protocols from [47] and [48] ...
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Lactylation-driven FTO-mediated m6A modification of CDK2 aggravates diabetic microvascular anomaliesbioRxiv - Cell Biology 2023Quote: ... NP-FB23-2 (3 mg/kg) was delivered through the retro-orbital sinus into neonatal mice with a 31-gauge insulin syringe (BD Biosciences, San Jose, CA, USA).
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bioRxiv - Pathology 2024Quote: ... Sections were incubated with a goat monoclonal antibody against EEA1 (1/100; SICGEN, Cantanhede, Portugal) and Caveolin 3 (1/750, BD Biosciences, Pont de Claix, France) antibodies (for dog sections ...
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bioRxiv - Immunology 2020Quote: ... In experiments testing double recombinants SARS-CoV2 vectors IL-2 antibody was not included and PE-CF594 anti-mouse IL-4 (clone 11B11, 562450, BD) and BV421 rat anti mouse IL-10 (clone JES5-16E3 ...
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bioRxiv - Genomics 2021Quote: ... stimulated cells were stored at 4°C until staining with combinations of fluorochrome-conjugated monoclonal antibodies including: anti-CD3 (SP34-2: Alexa700; BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... then fixed in 2% paraformaldehyde and stored at 4°C until acquisition on a FACSAria Fusion III flow cytometer (BD). Flow cytometry data was analysed with FlowJo version 9.9.6 (Tree Star).
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bioRxiv - Cancer Biology 2024Quote: ... with 4′,6-diamidino-2-phenylindole (DAPI) as a viability dye and the following antibodies: anti-CD31 PE (BD Biosciences), anti-CD45 APC (Fisher Scientific) ...
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bioRxiv - Immunology 2023Quote: ... and 1 μg/ml 4′,6-diamidino-2-phenylindole (DAPI) and analyzed on a BD FACS Aria III (BD Biosciences).