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Citations for Becton, Dickinson and Company :
601 - 650 of 7323 citations for 1 2 4 4 4 Pentachloropentafluorobutane since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... with blockade of cytokine secretion being achieved by simultaneous treatment with 4 µl of GolgiStop (# 51-2092KZ, BD Biosciences) for every 6 ml of cell culture for the last 3 hours ...
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bioRxiv - Developmental Biology 2023Quote: ... dissociated cell clusters were fixed for 20 min at 4°C in cold BD fixation/permeabilizationTM solution (BD Bioscience). Cells were washed twice in BD Perm/WashTM Buffer (BD Bioscience ...
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bioRxiv - Biophysics 2022Quote: ... The tissue sections were incubated at 4 °C overnight with rat anti-mouse CD31 (cat. no. 553370, BD Biosciences) and rabbit anti-cleaved Caspase-3 (cat ...
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bioRxiv - Microbiology 2022Quote: ... Cells were fixed with 4% formaldehyde solution and fluorescence was evaluated on a LSRII (BD Bioscience, San Jose, CA).
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bioRxiv - Immunology 2023Quote: ... Cells were labeled for 20 min at 4 °C with the following specific antibodies: BUV496 anti-CD19 (SJ25C1;BD), BUV563 anti-CD56 (MY31;BD) ...
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bioRxiv - Immunology 2023Quote: ... and IELs were stained in 2.4G2 supernatant for 30 minutes at 4°C with the following antibodies: (BD Biosciences) CD4 (RM4-4) ...
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bioRxiv - Immunology 2024Quote: ... 4°C for 10 min and binding was analyzed by flow cytometer FACS LSRFortessaTM (BD Bioscience, Franklin Lakes, NJ) using Yellow/Green Ex 561 nm Em 586/15 laser for the detection of antibodies ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 4 mg/ml heat-killed Mycobacterium tuberculosis H37Ra (400 μg; Becton, Dickinson and Company, Sparks, MD, USA) as described previously [21-23] ...
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bioRxiv - Neuroscience 2024Quote: ... 0.78 mM EDTA) and incubated for 30 min at 4 °C with FACS buffer containing Fc block (BD Biosciences) and primary antibody mix ...
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bioRxiv - Molecular Biology 2024Quote: ... Single cells were stained with the appropriate antibodies to surface markers at 4°C for 30 minutes in the dark.The following fluorescent dye-labeled antibodies purchased from BD Biosciences ...
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bioRxiv - Neuroscience 2024Quote: ... GFP-positive cells were sorted into 96-well PCR plates at 300 cells per well (3 wells per genotype) using a 4-way purity mask with the FACSDiva software v9.0.1 (BD Biosciences).
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bioRxiv - Neuroscience 2024Quote: ... HUVECs grown for indicated time periods on 0.1% gelatin-coated glass chamber slides (4- or 8-well; BD Falcon) in FA-supplemented or control media were washed once in PBS and fixed for 10 min on ice with 4% PFA ...
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bioRxiv - Neuroscience 2024Quote: ... in flotation for 1 h at room temperature and then overnight at 4°C with the mouse antibody that recognizes protein GM130 (Cat# A-610822, RRID:AB_398141, BD Biosciences), followed by incubation for 2 h at room temperature with Alexa Fluor 546-conjugated goat anti-mouse secondary antibodies (Cat#A-11030 ...
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bioRxiv - Neuroscience 2024Quote: The full 750-800µL suspension was loaded at 4°C into a FACSAria™ Fusion flow cytometer (BD Biosciences) with a 70 µm nostril running regular 1X PBS at the Flow Cytometry Core Facility of the Molecular Biology Department at Princeton ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated with primary antibody solutions for 5 days at 4°C: mouse monoclonal antiNMDAR1 (GluN1) (1:200; 556308, PharMingen, Becton, Dickinson and Company, Frankline Lakes, USA) or rabbit polyclonal antiNMDAa1 (GluN2A ...
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bioRxiv - Cancer Biology 2024Quote: ... MM1.S cells incubated overnight in the presence of Cy5-labeled DNA ODN were fixed with 4% paraformaldehyde and then labeled with anti-LAMP1 antibody (BD, cat no. 555798, 1:100 dilution) followed by staining with 1 µg/mL DAPI.
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bioRxiv - Systems Biology 2020Quote: ... Cells were stained at 4°C for 15 minutes with an APC-anti-Human-CD62L (clone DREG-56, BD Pharmingen), a BV650-anti-Human-CD11b (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... single cell suspensions were stimulated with OVA peptide (SIINFEKL) 2µM 37°C for 4 hrs in the presence of Golgi Stop (monensin, BD Biosciences). Upon extracellular staining ...
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bioRxiv - Genetics 2021Quote: ... and of the 12 mutant strains (4 reporter genes x 3 mutations) was quantified using a Sony MA-900 flow cytometer (the BD Accuri C6 instrument used for other fluorescence assays was not available due to Covid-19 shutdown ...
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bioRxiv - Molecular Biology 2021Quote: ... then cells were collected and 60 μg of sonicated chromatin was incubated overnight at 4°C with 20 μl of the anti-BrdU antibody (347580 Pure BD). Then ...
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bioRxiv - Neuroscience 2022Quote: ... and the following antibody mixture in PBS at 4°C for 30 minutes: BV421 Rat Anti-CD11b (BD Biosciences 562605), BV510 Hamster Anti-Mouse TCR β Chain (BD Biosciences 563221) ...
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bioRxiv - Immunology 2021Quote: ... for 4 h at 37°C in the presence of both Brefeldin A (GolgiPlug) and Monensin (GolgiStop, both BD Biosciences). As GolgiPlug and GolgiStop were used together ...
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bioRxiv - Immunology 2021Quote: ... followed by 200 μL FACS buffer before resuspending the cells in FACS buffer for acquisition in a high-throughput sampler on a 4-laser LSR Fortessa (BD), at a flow rate of 1 μL/s.
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bioRxiv - Immunology 2021Quote: ... cells from tumors were cultured ex-vivo from tumors for 4 hours in the presence of Golgi Plug (BD Biosciences) prior to cell surface and intracellular staining.
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... cells were washed again and re-suspended in permeabilization buffer with the following fluorescently-tagged antibodies targeting intracellular T-lymphocyte proteins for 30 min: IL-4 BV 421 (BD Biosciences clone 11B11 ...
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bioRxiv - Microbiology 2021Quote: ... Cells were washed twice (PBS) and fixed with 500 μl of 4% PFA and analysed by flow cytometry (BD Bioscience). A total of 10,000 events were acquired and data were analysed using FlowJo software version 10.6.2.
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated with the following antibodies for 30 min at 4°C: CD38 (Cat#342371; RRID: AB_400453, BD Biosciences), lineage cocktail (CD3/14/16/19/20/56 ...
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bioRxiv - Cancer Biology 2022Quote: Wells with the highest proportion of target barcodes were revived into a 6 well plate and grown for 4 days before being single-cell sorted by BD FACSAria II into 96 well plate ...
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bioRxiv - Molecular Biology 2022Quote: ... Primary or conjugated antibodies were incubated with the cells overnight at 4°C in Perm/Wash buffer (554714, BD Biosciences) containing 4% FBS and secondary antibodies for 45 min at RT ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by incubation for 25 min at 4° C with the following mouse specific antibodies to delineate subpopulations: CD24-PE (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... The cells (2-4 x 105 cells per well of a 96-well plate) were added to the ELISpot plate containing an immobilized primary antibody to either IFN-g or IL-4 (BD Cat# 551881 and BD Cat# 551878 ...
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Three Distinct Transcriptional Profiles of Monocytes Associate with Disease Activity in SSc PatientsbioRxiv - Genomics 2022Quote: ... and stained with the following cocktail for 30 minutes at 4°C in the dark to enable delineation of mature mononuclear phagocytes: Anti-CD45 PE-Cy7 (BD), anti-HLA-DR eFluor 450 (eBioscience) ...
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bioRxiv - Immunology 2022Quote: ... Antibodies to intracellular proteins were added to samples for 20 minutes at 4°C in the dark and then washed with PermWash (BD). Flow cytometry analysis was performed using a LSRFortessa flow cytometer (BD ...
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bioRxiv - Biophysics 2020Quote: ... The samples were kept in the dark at 4°C until analyzed using a LSR II flow cytometer (BD Biosciences). Hoechst 33342 and Pyronin Y staining were measured with a UV (350 nm ...
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bioRxiv - Developmental Biology 2021Quote: ... generated by hanging drops culturing of ESCs (1,000 cells per drop) were collected after 4 days and embedded in growth-factor-restricted Matrigel (MTG; BD Biosciences); 50µl MTG drops (containing around 20 EBs ...
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bioRxiv - Cell Biology 2020Quote: ... intranuclear staining was performed at 4 °C overnight in the dark using the FITC anti–human Ki-67 antibody (BD) diluted in Perm/Wash ...
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An innovative hematopoietic stem cell gene therapy approach benefits CLN1 disease in the mouse modelbioRxiv - Neuroscience 2022Quote: ... and labeling at 4°C for 15 minutes with the following specific antibodies: rat APC.Cy7 anti-mouse CD45 (BD Pharmingen) 1:100 ...
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bioRxiv - Immunology 2022Quote: ... Fluorescent bead uptake was measured in 4% paraformaldehyde (PFA) fixed cells by flow cytometry on a BD LSRII (BD Biosciences) and analyzed by FlowJo 10.3 ...
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bioRxiv - Immunology 2022Quote: ... were used to stain the cells in flow cytometry staining (FACS) buffer for 30 min at 4 °C before being washed and analyzed by BD FACS Array software™ on a BD FACS Array flow cytometer (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... Cells were incubated with antibodies against surface antigens for 20 minutes at 4°C and fixed with Stabilising Fixative (BD) prior to acquisition (52) ...
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bioRxiv - Immunology 2020Quote: ... BV421 conjugated streptavidin was added for 20 minutes at 4 °C prior to fixation and permeabilisation (BD Fix perm kit). Anti IFN-γ-FITC (clone CC302 ...
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bioRxiv - Physiology 2020Quote: ... Cell pellets re-suspended in 500μl PBS at 4°C were analysed using a BD 5L LSR Fortessa and BD FACSDiva software (BD Biosciences). To exclude dead cells ...
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bioRxiv - Immunology 2020Quote: ... the levels of IFNg expression was examined by intracellular staining after exposure to 4 hours of GolgiStop and GolgiPlug (BD).
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bioRxiv - Molecular Biology 2020Quote: ... 200 μl of ice-cold Solution C were added to the samples and cells were incubated for 10 minutes at 4°C in the dark before analysis with FACS CantoA (BD). The percentage of cells in each stage of the cell cycle (G1 ...
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bioRxiv - Immunology 2020Quote: ... The levels of IFNg protein expression was determined by intracellular staining after exposure to 4 hours of GolgiStop and GolgiPlug (BD).
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bioRxiv - Immunology 2020Quote: ... Cells were then labelled in 300 μl staining buffer using combinations of different antibodies for 30 min at 4 °C in the dark (anti-mouse CD4, CD69 (BD Bioscience ...
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bioRxiv - Immunology 2021Quote: ... Cells were collected and fixed by 4% PFA and subjected to analysis on a FACS Fortessa flow cytometer (BD Bioscience). Cells with GFP signal positive were considered as the virus-infected cells.
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bioRxiv - Immunology 2021Quote: Murine IFNγ and IL-4 ELISPOT assays were carried out according to the manufacture’s protocols for mouse IFNγ and IL-4 ELISPOT kit (BD Bioscience). Immunized mice splenocytes were seeded in the plates with a density of 2 × 105 cells per well and incubated with the peptide pool of 15 mer peptides with 11 overlapping amino acids for SARS-CoV-2 RBD protein (5 μg/mL ...
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bioRxiv - Immunology 2020Quote: ... IFN-γ and IL-4 protein in culture supernatants were quantified using the Cytokine Bead Array according to the manufacturer’s instructions (BD Biosciences)
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bioRxiv - Immunology 2023Quote: ... Cytokine production (IFN, IL-4, IL-17A, IL-10) was also assessed by Cytometric Bead Array (CBA) Kit (BD Biosciences) on supernatants collected at day 3 of polarization ...