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Citations for Becton, Dickinson and Company :
6251 - 6300 of 8729 citations for Elongator complex protein 1 ELP1 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Cells were subsequently stained for 20 min a 4°C with the appropriate combinations of fluorochrome-conjugated anti-human antibodies for the identification of CD8 T cells and sub-populations: CD3 BV480 (clone UCHT1, BD Biosciences), CD8 BV421 (clone RPA-T8 ...
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bioRxiv - Immunology 2023Quote: ... purified NK cells were incubated for 10 minutes at room temperature with 2.5µg of Human BD Fc Block followed by addition of 10μg/mL of anti-NKG2D purified antibody (BD Biosciences, clone 1D11) or isotype control antibodies prior to addition of SVG-A cells ...
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bioRxiv - Cell Biology 2023Quote: ... and subsequently hLSECs were purified via FACS by labelling with mouse anti-human CD31-FITC conjugated antibody (clone WM-59, BD Biosciences). Two independent preparations of transplanted iEC were used for single cell RNAseq ...
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bioRxiv - Bioengineering 2023Quote: ... At the end of the co-culture the cells were washed twice and incubated with human or mouse Fc receptor blocking antibodies (BD Biosciences). Human macrophages were stained with anti-CD11b (M1/70 ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were then washed and stained using anti-mouse monoclonal antibodies: CD45.1 Brilliant Violet 510 (clone A20, BD Pharmingen, RRID: AB_2739150), CD45.2 FITC (clone 104 ...
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bioRxiv - Immunology 2023Quote: ... Pooled cells from n = 3 - 5 mice/cohort were stained with PE- or APC-conjugated antibodies (from either BD or BioLegend) against murine F4/80 (clone ...
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bioRxiv - Immunology 2023Quote: ... and the 2D effective affinity AcKa was estimated by normalizing
against mr and ml that were measured using PE-labeled monoclonal antibody together with QuantiBRITE PE standard beads (BD Biosciences): -
bioRxiv - Immunology 2023Quote: ... Blue staining of PCNA was achieved by using a species-cross reactive mouse IgG2a-kappa monoclonal antibody directed against PCNA (clone PC10; BD Bioscience) and the Vector® Blue Substrate Kit ...
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bioRxiv - Genomics 2023Quote: ... for 5 minutes and stained at a dilution of 1:50 with one of two panels of directly conjugated antibodies for 30 minutes at 4 °C: anti-human CD45 (BD 563792), CD204 (Biolegend 371906) ...
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bioRxiv - Immunology 2023Quote: ... Cells were then permeabilized with BD Fix/Perm for 20 min at room temperature (RT) and stained intracellularly with antibodies in 1x Perm buffer (BD Biosciences): anti-IFN-γ (1:100 dilution ...
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bioRxiv - Immunology 2022Quote: Single-cell suspensions from colonic lamina propria were stained with fluorescently labelled antibodies and isolated using a BD FACSAria III cell sorter (BD Biosciences). Live CD45+ Lin- CD127+ ILC FACS sorted as KLRG1+ and KLRG1- were cultured in DMEM supplemented with 10% FCS ...
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bioRxiv - Immunology 2022Quote: ... Cell subsets for the populations from different donors were labelled simultaneously with the cell surface sorting panel (Table S4) an oligonucleotide-tagged antibody sample tag (BD Biosciences) prior to cell sorting on a FACSAria3 (BD Biosciences ...
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bioRxiv - Cancer Biology 2022Quote: ... CD137 expression on CD8+ T cells was assessed after an overnight co-culture with an anti-CD137 PE antibody (BD Biosciences).
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bioRxiv - Molecular Biology 2022Quote: ... The CldU and IdU tracks were detected by IF with anti BrdU antibodies (rat for CldU, Abcam ab6326, and mouse for IdU, BD 347580). Fiber integrity was assessed with a mouse anti-ssDNA antibody (Millipore MAB3034) ...
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bioRxiv - Cell Biology 2022Quote: ... or anti-β2 mAb IB4 (10 μg/ml) followed by Alexa Fluor 488-conjugated secondary antibody and analyzed using a FACScan (BD Biosciences) and Attune NxT flow cytometer (ThermoFisher) ...
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bioRxiv - Immunology 2024Quote: ... sorted T3a and T3b B cells were assayed for differentiation to FO at 24 hours in culture by flow cytometry using MitoTracker staining in conjunction with the following primary antibody panel: CD38 (HIT2, BD Biosciences), CD24 (ML5 ...
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bioRxiv - Microbiology 2023Quote: ... We then washed the cells and stained with FACS antibodies (anti-IL-2 FITC [BD Biosciences, 562040], anti-TNF PE [BD Biosciences, 554419], anti-IFN-γ [BD Biosciences ...
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bioRxiv - Biochemistry 2023Quote: ... 1 µg of this genomic DNA from soluble fraction was mixed with 3 µg of anti-IdU/BrdU antibody (3D4 clone, BD Science) in BrdU-IP buffer composed of PBS and 0.0625% Triton X-100 overnight ...
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bioRxiv - Immunology 2024Quote: MNCs from either IP or peak were immunophenotyped by FACS using the monoclonal antibodies CD3-FITC (BD Biosciences, San Jose, CA), CD4-FITC (BD Biosciences) ...
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bioRxiv - Neuroscience 2024Quote: ... and cells were blocked with 2% normal mouse serum and an anti-CD16/CD32 antibody (FcγR blocker, BD Pharmingen™ 553142) for 30 min on ice ...
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bioRxiv - Genomics 2024Quote: ... day 7 cells were transferred to fresh culture medium with IL-2 and stimulated again with peptides and 1ug/mL of anti-human CD28 antibody (BD Biosciences) for another 7 days ...
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bioRxiv - Developmental Biology 2024Quote: ... dissociated cells were stained with Alexa Fluor 647-conjugated rat anti-CD49f antibody (5% v/v in a 100 µL reaction; BD Biosciences) for 25 minutes at 37°C on an orbital shaker at 200 rcf and resuspended in DPBS containing 0.5% bovine serum albumin and 50 units/mL DNase I (Sigma Aldrich) ...
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bioRxiv - Immunology 2024Quote: ... cells were incubated with anti-CD16/32 APC-Fire750 and anti-CD64 PECy7 for 15 min on ice before the addition of the rest of the fluorophore-conjugated antibodies were added on ice for 20 min in Brilliant Stain Buffer (BD Biosciences). For GATA6 staining ...
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bioRxiv - Microbiology 2024Quote: ... Parasites were then washed with FACS buffer and placed on ice for incubation with anti-isotype detection antibodies depending on application: anti-IgG3-BV421 (R40-82, BD Bioscience), anti-IgM-PE/Cy7 (RMM-1 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell death was identified by analysis of cells stained with annexin V-(AnV-FITC) and propidium iodide (PI) antibodies before flow cytometry analysis as recommended (BD Biosciences).
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bioRxiv - Immunology 2023Quote: ... 0.25X and 4X concentration of CD4 T cell-depleting antibody for 30 minutes at 37°C before surface staining with CD3 (clone SP34-2, BD Pharmingen), CD4 (Clone L200 ...
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bioRxiv - Immunology 2024Quote: ... The maturity of differentiated HL60 cell was probed by CD11b expression on flow cytometry using anti-CD11b-FITC antibody (BD Biosciences). Differentiated HL60 cells were harvested and stimulated with 10 µg/mL of resistin for 16 hours ...
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bioRxiv - Immunology 2023Quote: ... TCR transduction efficiency was measured by flow cytometry using the PE a-mouse TCRβ antibody (clone H57-597, BD Biosciences 553172) and anti-PE MicroBeads (Miltenyi Biotec 130-048-801) ...
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bioRxiv - Immunology 2023Quote: ... The binding of GITRL or OX40L was detected using anti-FLAG APC antibody and measured on Accuri C6 cytometer (BD Bioscience). The data were analyzed using FlowJo software (BD Bioscience).
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bioRxiv - Cancer Biology 2023Quote: The antibodies used for flow staining in this study are: mouse anti-human CD3 conjugated to BUV737 (clone UCHT1, BD, 612750), mouse anti-human CD45 conjugated to FITC (clone HI30 ...
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bioRxiv - Biochemistry 2023Quote: ... (Antibody: pSTAT1: BD Phosflow 612597, pSTAT2: cell signaling 907405, pSTAT3: BD Phosflow 557815, pSTAT5: BD Phosflow 612599, pSTAT6: BD Phosflow 612601).
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bioRxiv - Neuroscience 2023Quote: ... while 100 μg was subjected to chromatin immunoprecipitation (ChIP) using 5 μg of mouse anti-ASCL1 (Mash1) antibody (BD Biosciences, 556604) or 5 μg rabbit ant-OLIG2 (Olig2 ...
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bioRxiv - Neuroscience 2023Quote: ... P2’ lysate (2 mg; 2 g/L) was incubated with either 2 μg of anti-mDia1 antibody (BD Biosciences, Cat# 610848) or equal amounts of immunoglobulin G (IgG ...
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bioRxiv - Immunology 2024Quote: ... surface antibodies were incubated with cells for 30 minutes at 4°C in hFACS buffer and Brilliant Stain Buffer (BD Biosciences). For intracellular staining ...
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bioRxiv - Synthetic Biology 2024Quote: ... or showed a complete loss of antibody binding (escape) (IgG-/FLAG+) were sorted by FACS (BD Aria Fusion or BD Influx). Collected cells were cultured in SD-UT medium for 16-48 hours at 30°C ...
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bioRxiv - Cancer Biology 2023Quote: ... or ASC52telo cells (ATCC SCRC-4000) were suspended in 5%FBS/PBS solution and the following antibodies were added: APC-CD14 (BD 561708), APC-CD19 (BD 561742) ...
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bioRxiv - Immunology 2023Quote: ... up to 2 x106 MR1-5-OP-RU-APC Tetramer magnetic bead enriched spleen (n= 2) or unenriched colon (n= 2) single cell suspensions were labeled with an oligonucleotide-tagged antibody sample tag (BD Biosciences). MAIT cells (MR1-5-OP-RU-Tet+TCRβ+CD3+ ...
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bioRxiv - Immunology 2023Quote: ... solution for 20 minutes followed by 20 minutes of antibody cross linking on ice with 2 µg/mL goat-anti mouse solution (BD, #53998). T cells were then activated via an incubation period at 37 °C in FACS buffer (PBS with 2% FBS and 2 mM EDTA ...
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bioRxiv - Immunology 2023Quote: ... Single-cell suspensions were incubated for 20 minutes with Tmem119 and Neun antibodies and afterwards sorted with the FACS sorter Aria Fusion (BD Biosciences) at the Institute for Biomedical Aging Research ...
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bioRxiv - Cancer Biology 2023Quote: ... lineage-negative cells were stained with antibodies for the isolation of HSPCs followed by FACS using BD FACS Aria II and III cell sorters (BD Biosciences).
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bioRxiv - Immunology 2023Quote: ... the plate was washed 5x with PBST 0.05% and incubated with biotinylated antibody G1 (Serotec MCA336B) or G2A (BD bioscience 553388) for 60min at RT ...
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bioRxiv - Physiology 2024Quote: ... endothelial cells were purified by two consecutive immuno-selection procedures using magnetic beads conjugated with anti-ICAM-2 antibody (BD Pharmingen) and plated into 75 cm2 tissue culture coated flasks ...
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bioRxiv - Immunology 2022Quote: ... cells were fixed with fixation buffer and then permeabilized with Cytofix / Cytoperm (e-bioscience) followed by staining for 60 minutes with antibodies that were diluted in 1X perm buffer (BD, 554723). Flow cytometry data acquisition was performed either on LSR Fortessa X-20 (BD ...
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bioRxiv - Immunology 2022Quote: ... Mouse and rat anti-human monoclonal antibodies (mAbs) used in this study included: IFNγ FITC (clone 25723.11, BD Biosciences, cat# 340449), TCRαβ Percp 710 (clone IP26 ...
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bioRxiv - Cancer Biology 2022Quote: ... and engraftment of human cells in the bone marrow and spleen was measured with flow cytometry (CD45-PE-Cy7 antibody, BD Biosciences). Single-cell suspensions were prepared from the bone marrow ...
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bioRxiv - Cell Biology 2022Quote: ... Phenotypic analysis was performed with the following antibodies: Rat anti-mouse active β1-integrin (active CD29 Clone 9EG7, 553715; BD Biosciences); Alexa647-Armenian hamster anti-mouse β1-integrin (total β1-integrin 102214 ...
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bioRxiv - Cell Biology 2022Quote: ... The intracellular staining was carried out according to the manufacturer’s instructions using Fixation/Permeabilization kit (eBioscience) in the presence or absence of antibodies against OCT3/4 (OCT4) (AlexaFluor 647 mouse anti-OCT3/4, BD Biosciences), SOX17 (APC Goat anti-SOX17 ...
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bioRxiv - Cancer Biology 2022Quote: ... incubated with FITC-conjugated anti-BrdU antibody and then analyzed by flow cytometry with a BD FACSCanto II system (BD Biosciences). Data are shown as mean ± standard deviation and are representative of three or four independent experiments ...
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bioRxiv - Immunology 2022Quote: ... stained on ice for 30’ with relevant cocktails of antibodies and populations of interest were sorted at 4°C on an BD FACSARIA (BD Bioscience) using a 70 μM nozzle in 50% FBS RPMI ...
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bioRxiv - Immunology 2022Quote: ... intracellular cytokine staining was performed at 4°C for 30 minutes using a cocktail of antibodies resuspended in 1x Perm/Wash buffer (BD Biosciences); intracellular antibodies included IFNγ APC-Cy7 (BioLegend ...