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Citations for Becton, Dickinson and Company :
6151 - 6200 of 9171 citations for 7 7a Dihydro 2 2 4 6 6 pentamethyl 1 3 benzodioxol 5 6H one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... mMDSCs (CD11b+Gr-1+Ly6G-) and gMDSCs (CD11b+Gr-1+Ly6G+) were sorted into FACS buffer using a BD FACSMelody (BD Bioscience). After centrifugation at 400 g for 5 min ...
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bioRxiv - Cell Biology 2022Quote: ... Unfractionated BM was diluted 1:1 in DMEM with 10% FBS and added to vacuum gas plasma treated plates (BD Falcon). After three days ...
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bioRxiv - Neuroscience 2022Quote: ... that was blocked in 5% non-fat milk for 1 h at room temperature and incubated overnight with primary antibodies against the following proteins: α-synuclein (1:1000, BD), NCK2 (1:1000 ...
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bioRxiv - Molecular Biology 2022Quote: ... The cells were stained with the antibody cocktail in the staining buffer of a 1:1 mix of PBS and Brilliant Staining Buffer (BD, 566349), at room temperature for 20 mins or at 4°C for 30-40 mins ...
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bioRxiv - Neuroscience 2023Quote: ... and antibodies directed against intracellular targets Foxp3 (APC, eBioscience #17-5773, 1:200) and RORγt (BV421, BD Biosciences #562894, 1:200). After 1h incubation at 4°C ...
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bioRxiv - Physiology 2024Quote: ... The 2X stain cocktail included the following antibodies (final dilutions are all 1:300 unless otherwise indicated): rat anti-mouse SiglecF-BV421 (1:400, clone E50-2440, cat# 562681 BD Horizon), rat anti-mouse/human CD11b-Pacific Blue (clone M1/70 ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then washed 2x with 1% FBS in PBS and incubated in 1% FBS in PBS with APC-conjugated mouse anti-human CD68 (BD Biosciences) for 1 hour at room temperature ...
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bioRxiv - Immunology 2024Quote: ... PMA [1μg/ml] and ionomycin [0,5μg/ml] with Golgi Plug [1:1000] and Golgi Stop [1:1000] solutions (Cytofix/Cytoperm TM Plus, BD Bioscience, ref: 555028) for 4-5h at 37°C to block Golgi’s exocytosis ...
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bioRxiv - Microbiology 2023Quote: ... for 16 hours followed by stimulation with a peptide pool of a broad range of HLA-subtypes and infectious agents (CEFX Ultra SuperStim Pool; JPT) (1 µg/ml) and 1 μg/ml mouse-anti-human CD28 antibody (#556620; BD Biosciences), or IL-2 (20 IU/ml ...
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bioRxiv - Cancer Biology 2024Quote: ... 3 × 106 VCaP cells were subcutaneously injected into the dorsal flank on both sides of each mouse in a 1:1 mixture of serum free medium and Matrigel (BD Biosciences). Once tumors grew to a palpable size (∼400 mm3) ...
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bioRxiv - Immunology 2023Quote: ... cells were stained intracellular with anti-Foxp3 Alexa Fluor 488 (eBioscience; 1/100) and anti-ROR t BV421 (BD; 1/100). Flow cytometry data were analyzed using FlowJo Software 10.8.1 and a sequential gating strategy ...
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bioRxiv - Molecular Biology 2023Quote: ... For BrdU staining we used either a rat anti-BrdU (1:1000, Eurobio clone BU1/75) or mouse anti-BrdU (1:1000, BD 347580) antibodies 2 hours at RT (1:1000 ...
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bioRxiv - Immunology 2023Quote: ... The cells were washed with buffer (0.2% FBS in 1× PBS) and data (∼1 million events/sample) was acquired using BD LSRFortressa X-20 (BD Biosciences, USA). The acquired flow cytometry data were analysed using FCS Express (DeNovo software ...
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bioRxiv - Immunology 2023Quote: ... together with PE-anti-PD-L2 (clone TY25, 1:20) and its isotype control PE-Rat IgG2a,κ (clone R35-95, 1:20) were purchased from BD Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: ... were anesthetized with isofluorane and GFP-Luc labelled cells or MCF7-HypT cells resuspended in 1:1 PBS and Matrigel (BD Biosciences) were injected into the fourth mammary fat pad ...
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bioRxiv - Developmental Biology 2022Quote: ... 1:10000), anti-dendritic cell mAb: anti-CD11c (N418)-FITC (11-0114-81, eBioscience; 1:20) using LSR II (BD Biosciences). For OC differentiation ...
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bioRxiv - Cancer Biology 2023Quote: ... coverslips were incubated with primary antibodies (mouse anti-HA 1:200, Cell Signaling Technology, 2367; mouse anti-CtBP2, 1:250; BD Biosciences Cat# 612044 ...
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bioRxiv - Immunology 2023Quote: ... cells were stained for 30 min on ice with biotinylated recombinant HAs and PD-1-BB515 (EH12.1, BD Horizon, 1:100) diluted in P2 ...
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bioRxiv - Molecular Biology 2023Quote: ... 5.0 × 104 MDA-MB-231 or HCC1806-LM2C triple reporter-labeled cells were resuspended in a 1:1 mixture of PBS and Matrigel (BD Biosciences), and injected into the 4th mammary fat pad of female NOD-SCID-g female mice 6-8 weeks of age ...
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bioRxiv - Cancer Biology 2023Quote: ... and/or 0.25 million A431 cells in a 100 μL cell suspension of a 1:1 (v/v) mixture of medium with reconstituted basement membrane (BD Matrigel, BD Biosciences). Mice-bearing NCIN87/A431 bilateral tumors were developed by subcutaneous injection of NCIN87 and A431 cancer cells on the bilateral dorsal flank regions ...
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bioRxiv - Microbiology 2023Quote: ... Crypts in the tube were collected after removing the supernatant, and resuspended in 50% L-WRN conditioned medium (CM, Table S3) mixed 1:1 with Matrigel (BD Biosciences). The crypt suspension (55 μl per well ...
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bioRxiv - Immunology 2023Quote: ... and anti-MCL-1 A647 (1:1000, clone 19C4-15, WEHI antibody facility) and analysed on a Fortessa X20 (BD Biosciences) or Cytek Aurora spectral flow cytometer (Cytek Biosciences) ...
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bioRxiv - Microbiology 2024Quote: ... washed with PBS-T and kept overnight in 1% BSA solution in PBS-T containing a 1:500 dilution of anti-rabbit-Vi-polysaccharide antibody (BD BioSciences) overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... The cells were resuspended in T cell stimulation media (RPMI 1640 supplemented with 10% FBS, 1% Penicillin/Streptomycin, Protein Transport Inhibitor (Containing Brefeldin A) (diluted 1:1000) (BD, 555029), Monensin (Biolegend ...
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bioRxiv - Immunology 2020Quote: Multiscreen ninety-six well plates were coated overnight with 100 μl per well of 5 μg/ml anti-human interferon-γ (IFN-γ) (B27; Becton, Dickinson and Company, Franklin Lakes, NJ) in endotoxin-free Dulbecco’s-PBS (D-PBS) ...
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bioRxiv - Cancer Biology 2021Quote: ... and resuspended in 50 µL of FACS buffer (PBS plus 1% BSA) and blocked with µL 1 human Fc block (BD Biosciences, 564219) for 20 minutes at 4°C ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated for 30 min on ice with biotinylated and Alexa Fluor 647-conjugated recombinant soluble S and PD-1-BB515 (EH12.1, BD Horizon, 1:100) in 2% FBS and 2 mM EDTA in PBS (P2) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were fixed and permeabilized prior to staining with primary antibody (Vimentin CST # 1:100 and E-cadherin BD number 1:100) overnight followed by secondary (anti-rabbit ThermoFisher #31466 1:10000 ...
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bioRxiv - Neuroscience 2019Quote: ... Blocking was followed by 1-hour incubation with Anti-Pan Nav (Alomone labs, # ASC003) (1:50) and purified Mouse Anti-MAP II (BD biosciences, #556320) (1:400 ...
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bioRxiv - Neuroscience 2020Quote: ... one-in-ten sections were incubated with different anti-BrdU antibodies (BrdU & CldU, rat primary antibodies at 1/200 Accurate Chemical; IdU, mouse primary antibodies at 1/200, BD Biosciences). Sections were also incubated with Zif268 (rabbit,1:500 ...
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bioRxiv - Immunology 2021Quote: ... 1 mM ethylenediaminetetraacetic acid (EDTA)) with the following anti-human antibodies (all at 1:200 dilution): anti-CD20-PECy7 (BD Biosciences, 335793), anti-CD3-APC-eFluro 780 (Invitrogen ...
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bioRxiv - Immunology 2021Quote: 12-week old mice were sensitized by intradermal injection of 100ug rat collagen type II (COL2) in 100ul of a 1:1 emulsion with Complete Freund’s adjuvant (BD, Difco, MI, USA) and 10mM acetic acid at the base of the tail ...
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bioRxiv - Immunology 2022Quote: ... 1 mM ethylenediaminetetraacetic acid (EDTA)) with the following anti-human antibodies (all at 1:200 dilution): anti-CD20-PECy7 (BD Biosciences, 335793), anti-CD3-APC-eFluro 780 (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... 100 µL of each collected serum sample diluted 1:100 with 1% (w/v) Difco Skim Milk (cat. n° 232100, BD Biosciences, USA) was added to each plate for 1 h at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... rat anti C4b (thermo fisher scientific MA1-40047,1:25) rabbit anti Olig2 (millipore AB9610, 1:250) mouse anti-Gstp (BD 610719, 1:250), anti mouse 555 (thermo fisher scientific A-21422 ...
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bioRxiv - Immunology 2022Quote: ... 1 mM ethylenediaminetetraacetic acid (EDTA)) with the following anti-human antibodies (all at 1:200 dilution): anti-CD20-PECy7 (BD Biosciences, 335793), anti-CD3-APC-eFluro780 (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... washed with PBS and incubated in DMEM media containing 1% FBS and 1% BSA with Alexa Fluor® 647 Mouse Anti-Human LAMP1 Clone H4A3 (BD Biosciences) for 1h at 4°C with intermittent mixing ...
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bioRxiv - Neuroscience 2021Quote: ... The cell pellet was then stained with antibodies to microglial cell surface markers (CD11b-PE Cy7, 1:200 Biolegend, # 101216; CD45-APC Cy7, 1:200, BD Biosciences # 103116;) for isolation using the FACSAria™ III cell sorter (Fig 1C) ...
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bioRxiv - Cancer Biology 2021Quote: ... The fibres were stained with primary antibodies (Rat anti-BrdU Abcam ab6326 1:250, Mouse anti-BrdU BD Biosciences 347580 1:100) overnight and with secondary antibodies (Alexafluor 555 goat anti-rat 1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti-Tuj1 (gift from Tony Spano, University of Virginia, 1:250) and rat anti-Icam-II (BD Pharmingen, 553325, 1:500). Secondary antibodies ...
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bioRxiv - Bioengineering 2022Quote: ... the membrane was incubated overnight at 4°C or with primary antibody solution (1:1000 dilution for anti-Alix [E4T7U, Cell Signaling Technology], anti-QSOX1 [12713-1-AP, ProteinTech], anti-HIF-1α [610958, BD Biosciences] ...
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bioRxiv - Immunology 2022Quote: ... 1 mM ethylenediaminetetraacetic acid (EDTA)) with the following anti-human antibodies (all at 1:200 dilution): anti-CD20-PECy7 (BD Biosciences, 335793), anti-CD3-APC-eFluro 780 (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... The cells were washed 1× with 1ml cold PBS and resuspended in 500μl PBS containing PE Streptavidin (BD 554061: 1:200 dilution) and Zombie Violet live dead stain (BioLegend 423113 ...
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bioRxiv - Immunology 2022Quote: ... Then the cells underwent membrane surface staining for 30 minutes at 4°C protected from light with Fixable Viability Dye eFluor506 (1:1000, eBiosience, Cat# 65-0866-14) and the following antibodies: CD3 BUV805 (1:50, BD, Cat# 612895), CD8 BUV496 (1:50 ...
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bioRxiv - Neuroscience 2024Quote: ... The following primary antibodies were used in this study: anti-total α-syn mouse monoclonal Syn-1 (1:10,000 dilution; BD Biosciences #610786), anti-PSyn rabbit monoclonal EP1536Y (1:10,000 dilution ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were cultured in RPMI plus 10% FBS in 2D on tissue culture plates or in 3D in non-adherent suspension plates in a 1:1 mix of Matrigel (growth factor-reduced, BD Biosciences - 354230) and RPMI plus 10% FBS ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were blocked in 5% milk in 0.2% Tween-20 in TBS for 1 h at room temperature and probed with primary antibodies against SORLA (1:4000; 611680, BD Transduction Labs) and GAPDH (1:10,000 ...
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bioRxiv - Immunology 2023Quote: ... DO11.10 TCR transgenic mice subcutaneously received 50 μL injections of a 1:1 emulsion of adjuvant complete Freund H37 Ra (BD Difco, 231131) and 2 mg/mL grade V ovalbumin (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... FITC-coupled anti-ST2 (1:100, clone DJ8, MD Bioproducts, #101001F) and PE-coupled anti-CD13 (1:100, clone R3-242, BD Pharmingen, #558745) antibodies ...
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bioRxiv - Immunology 2023Quote: ... or FITC-coupled anti-ST2 (1:100, clone DJ8, MD Bioproducts, #101001F) and PE-coupled anti-CD13 (1:100, clone R3-242, BD Pharmingen, #558745), respectively ...