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Citations for Becton, Dickinson and Company :
551 - 589 of 589 citations for Pemetrexed disodium Impurity 30 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... incubated with 2M HCl for 30 minutes and stained with mouse monoclonal anti-BrdU antibody (1:50) (BD Biosciences, San Jose, CA, USA) and Alexa fluor-488 conjugated secondary antibody (1:200 ...
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bioRxiv - Immunology 2020Quote: ... Pellets were then stained with a panel of antibodies for 30 minutes (4°C) followed by washes in PBS before analysis by flow cytometry (BD, Franklin Lakes, NJ).
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bioRxiv - Neuroscience 2020Quote: Brains from paraformaldehyde-perfused 12 month-old mice were sectioned and 30 µm free-floating sections were incubated overnight at 4°C with primary antibodies (anti-αsyn, BD Transduction, 1:700), anti-DNAJC5 (Millipore ...
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bioRxiv - Immunology 2021Quote: We permeabilized formalin-fixed glomerular and tubulointerstitial single cells with 0.25% Tween-20 in PEB buffer at room temperature for 30 minutes and blocked cells with Fc Block (clone 2.4G2, BD Biosciences, San Jose, CA) diluted in 0.25% Tween-20 on ice for 10 minutes ...
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bioRxiv - Genomics 2024Quote: ... Antibodies were added to stain surface markers for 30 min at 4°C (CD33 BV711, BD; CD11b APC-Cy7, BD; Zombie Violet, BioLegend). After 30min ...
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bioRxiv - Immunology 2023Quote: Cells were first labelled with Fc block for 10 minutes at 4°C and then the following antibodies added: CD45-PE (BD, 30-F.11), Siglec F-APC (BioLegend ...
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bioRxiv - Bioengineering 2024Quote: ... for 10 min at room temperature and then stained for 30 min at 4°C with the following antibody cocktail: anti-human CD19 (Becton, Dickinson and Company, BD ...
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bioRxiv - Immunology 2023Quote: ... and incubated at 4°C for 30 min with a B cell surface marker antibody cocktail (Table S11) with 10 µl Brilliant Stain Buffer Plus (BD, cat. No. 566385) diluted in PBS containing 1% BSA up to a volume of 100 µl ...
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bioRxiv - Cancer Biology 2023Quote: ... and resuspended in 0.04 mL sterile PBS and injected under the splenic capsule with an insulin syringe (BD, MicroFine, 0.3 mL, 30 G). Alternatively ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then suspended in 0.5% Tween 20 (vol/vol) plus 1% BSA/PBS and incubated for 30 minutes at room temperature with anti-BrdU FITC (BD Biosciences, Cat. #347583) at a concentration of 0.5 µg/106 cells ...
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bioRxiv - Immunology 2024Quote: ... Cells were then washed and incubated in following antibodies for 30 minutes at 4°C in the dark: PerCP rat anti-mouse CD45 (557235, BD Biosciences, 1:50), FITC rat anti-CD11b (553310 ...
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bioRxiv - Immunology 2024Quote: ... Cell suspensions were then incubated for 30 min with anti-mouse antibodies (1:500 dilution) against CD45 Brilliant Violet 711 (0.2 mg/ml; Clone 30-F11, 563709, BD Biosciences, San Jose, CA), CD45 PE-Firetm700 (0.2 mg/ml ...
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bioRxiv - Neuroscience 2024Quote: ... 250,000-350,000 donor cells or the same volume of empty HBSS+2% FBS vehicle were intraperitoneally (IP) injected using a 30 G insulin syringe (BD Biosciences cat# 328431). Transplanted cells were allowed 2 weeks to engraft prior to SCI.
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bioRxiv - Bioengineering 2024Quote: The following antibodies were used for cell staining/blocking prior to the secretion incubation (20-30 minutes at 4°C in 2% FBS/PBS): i) one-cell discovery campaigns – Fc-block (5 μg/ml; BD Biosciences cat# 553142) and anti-mouse TACI-APC (3 μg/ml ...
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bioRxiv - Genetics 2021Quote: ... 0.5 mM EGTA and 30 mM Tris–HCl pH 7.4) using 25 ml total pushed through a 30 ml syringe with a 26G needle (BD Precision Glide, cat. number 305111) into the left ventricle of the beating heart ...
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bioRxiv - Immunology 2022Quote: ... and incubated for 30 min at 4°C with a cocktail of mouse anti-human antibodies: CD19 Alexa 700 (HIB19, BD Biosciences, San Jose, CA), CD21 BV421 (B-ly4 ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were fixed using 2% paraformaldehyde (PFA) solution for 30 minutes and permeabilization was performed using a 1X BD perm/wash solution (BD Biosciences, USA; cat#554723) for 15 minutes at 4°C ...
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bioRxiv - Immunology 2020Quote: ... cells were fixed and permeabilized for 30 min at 4 °C in the dark after extracelluar stainings using BD Cytofix/Cytoperm (Fixation and Permeabilization Solution, BD Biosciences, Cat: 51-2090KZ) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... The cells were incubated for 30 min at room temperature in the staining solution before being subjected to flow cytometry analysis (FACSCalibur, BD Biosciences, San Jose, CA).
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bioRxiv - Cancer Biology 2023Quote: ... APC-eFluor780-conjugated cKit and BV605-conjugated Fc Gamma (CD16/CD32) for 30 minutes followed by fixation and permeabilization using BD Cytofix/Cytoperm (BD Biosciences, San Jose, California). Intracellular staining using FITC-conjugated KI-67 was performed overnight at 4 degrees ...
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bioRxiv - Cancer Biology 2023Quote: ... 1x106 cells in 100 μL were stained for 30 minutes at room temperature using the following antibodies: CD90-BUV395 (BD, #740260, 0.1 mg/μL), ECAD-AF405 (Novus Bio ...
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bioRxiv - Molecular Biology 2022Quote: ... for 30 min at room temperature and analyzed by flow cytometry using a Becton Dickinson FACS Calibur flow cytometer (BD Biosciences, San Jose, CA). Cell cycle data were analyzed using CytExpert (Beckman Coulter Inc ...
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bioRxiv - Cell Biology 2024Quote: ... Cells from the stroma were sorted gating on single GFP-positive cells from PdgfrαH2Be-GFP mice or using anti-CD45-PE/Cy7 (BD Pharmingen, clone 30-F11, #552848) antibodies ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by incubation with primary antibodies for at least 1 hr at room temperature and secondary antibodies (for nonconjugated primary antibodies) for 30 min at room temperature with washes between the steps before analysis by flow cytometry (BD Fortessa or MACSQuant 16). To exclude dead cells ...
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bioRxiv - Microbiology 2024Quote: ... we rolled a subset of disinfested seeds on malt extract nutrient agar by shaking the plate for 30 s and subsequently removing seeds (BD Difco, Franklin Lakes, NJ). Plates were incubated at room temperature (25 ℃) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... for 30 min (RT) and incubated overnight (4 °C) with mouse anti-5-LOX (1:1000; # 610695; Lot: 9067817; BD Biosciences, Franklin Lakes, NJ) and rabbit anti-perilipin-2 (1:100 ...
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bioRxiv - Immunology 2024Quote: ... mice were anesthetized using isoflurane and injected retro-orbitally with CD45:PE (100 µL per mouse, diluted 1:20 in sterile saline; BD Biosciences, clone 30-F11) for intravital labeling of circulating CD45+ cells (Anderson ...
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bioRxiv - Neuroscience 2024Quote: ... we filled the stage with extracellular saline 95 and cut the vertex using a hypodermic needle (30 G, BD PrecisionGlide, Franklin Lakes, NJ USA). The cut began at the anterior head and followed the lines of bristles along the compound eyes ...
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bioRxiv - Bioengineering 2024Quote: ... and 2 mM MgCl2) and allowed to settle for 1–2 min on anti-CD3ε antibody (30 μg/ml, BD Pharmingen, clone 145-2C11) coated coverslips ...
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bioRxiv - Microbiology 2021Quote: ... sections were incubated for 30 minutes at room temperature with a rat monoclonal antibody anti-Mouse CD8 (BD Pharmingen, BD Bioscience, San Jose, CA, USA) diluted 1:50 in blocking solution ...
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bioRxiv - Microbiology 2020Quote: ... TMF and C6-NBD-cer fluorescence intensities were acquired on the FL1-H channel (530/30 bandpass filter) while PI fluorescence on the FL3-H channel (670LP filter) of a FACSCalibur (BD Biosciences, San Jose, CA, USA). Post-analysis was performed in the software Summit (version 4.3 ...
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bioRxiv - Microbiology 2021Quote: Expression of T3SS genes was measured in denitrification mutants grown in 40 mL of VDM + 30 mM nitrate grown in GasPak™ EZ microaerobic pouches (BD, Franklin Lakes, NJ, USA) at 28°C with shaking for 18 hours starting from an OD600 of 0.001 ...
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bioRxiv - Microbiology 2024Quote: ... Fixed/ permeabilized cells were incubated for 30 min on ice with: Alexa Fluor® 647-conjugated mouse anti-mouse T-bet (4B10; BD Biosciences cat no. 561264), PE-conjugated rat anti-mouse GATA3 (TWAJ ...
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bioRxiv - Immunology 2023Quote: ... 100 µL of whole blood was added to an antibody cocktail containing the following antibodies: mouse-anti-CD3-V500 (BD, catalog #560770, dil 1:30), mouse-anti-CD4-PerCP/Cy5.5 (BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... membrane staining was conducted for 30 min at 4°C using the following antibodies: CD8 BUV496 (2:100; BD Biosciences; clone RPA-T8; Cat# 612942), CD4 Brilliant Violet (BV ...
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bioRxiv - Neuroscience 2021Quote: ... and one minute later 100-150 μL of virus injected into the retro-orbital sinus using a U100 insulin syringe (BD micro-fine 0.3mL, 30 gauge needle). The volume was determined based on the animal’s weight15 ...
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bioRxiv - Immunology 2020Quote: ... Cells were then surface stained for 30 min in the dark at 4°C with viability reagent (BD Horizon™ Fixable Viability Stain, BD Biosciences, San Diego, CA, USA) and a 29-color cocktail of monoclonal antibodies (mAbs ...
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bioRxiv - Microbiology 2021Quote: ... Signals for 5 fluorescent markers were created by staining with antibodies against the common leukocyte antigen (Rat-anti-mouse CD45, Cat# 550539, clone: 30-F11, BD Pharmingen, San Jose, CA 95131, USA) indirectly labeled with a goat-anti-rat secondary antibody labeled with Alexa Fluor 488 ...
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bioRxiv - Bioengineering 2024Quote: ... for 10 min at room temperature and then stained for 30 min at 4°C with the following antibody cocktail: anti-human CD19 (Becton, Dickinson and Company, BD, Franklin Lakes, NJ, USA, cat.: 562440), anti-human CD20 (BioLegend ...