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Citations for Becton, Dickinson and Company :
551 - 600 of 2213 citations for CD19 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... and blocked with Fc Block (BD Biosciences, San José, CA) before staining with the following extracellular antibodies in FACS buffer ...
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bioRxiv - Immunology 2023Quote: ... crystallizable (Fc) receptors were blocked (anti-CD16/32, BD Pharmingen) prior to staining with LIVE/DEAD Fixable near IR Dead Cell Stain (Thermo Fisher) ...
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bioRxiv - Immunology 2023Quote: ... Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block) (553142) ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were resuspended in 20μl of Fc-blocker (BD Bioscienses) for 15 minutes ...
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bioRxiv - Immunology 2024Quote: ... containing 9.5µL staining buffer and 0.5µL Fc Block (BD Pharmingen) for 10min at 4°C ...
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bioRxiv - Immunology 2024Quote: ... Samples were first stained with Fc Block cocktail (BD Pharmingen) containing 9.5µL staining buffer and 0.5µL Fc Block (BD Pharmingen ...
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bioRxiv - Immunology 2024Quote: ... FCS files were exported from the Symphony A5 (BD Biosciences) flow cytometer and imported into the R environment using flowCore package (version 2.4) ...
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bioRxiv - Immunology 2024Quote: ... and cells were first blocked with Fc-block (BD 564220), stained with AF647-conjugated antibodies to CD4 (Biolegend 317422 ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by an Fc receptor block (BD Pharmingen, cat. 553142) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2024Quote: ... Cells were incubated with Fc block (1:250; BD Biosciences) and Fixable Viability Dye eFluor 506 (1:1000 ...
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bioRxiv - Genomics 2020Quote: ... Fluorochrome-conjugated antibodies against the following mouse antigens were then used for sorting by flow cytometry: CD45.2-FITC (clone 104) and CD19-PECy7 from BD Pharmingen ...
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bioRxiv - Immunology 2022Quote: ... CD19+ B cells were enriched from donor PBMCs via negative isolation (BD iMag Negative Isolation Kit, BD Biosciences #558007) and resuspended at 2×106 cells/mL in R15 supplemented with 2 mM L-glutamine ...
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bioRxiv - Immunology 2020Quote: ... CD19+ Pf+ or HA+ B cells were single cell–sorted into 96-well plates using a FACSAria II (BD). Index sorting was used to determine isotype and B cell subsets by IgM ...
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bioRxiv - Immunology 2020Quote: ... CD19+ B cells were extracted from each PBMC sample through magnetic separation (BD iMag Negative Isolation Kit, BD, # 558007). Purified B cells were cultured in RPMI 1640 media supplemented with 15% fetal calf serum (FCS ...
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bioRxiv - Immunology 2023Quote: ... clone 53-6.7), CD19 (V450, clone: 1D3), CD71 (PE) and IFN-γ(BV421, clone: XMG1.2) were purchased from BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... Cells were labeled for 20 min at 4 °C with the following specific antibodies: BUV496 anti-CD19 (SJ25C1;BD), BUV563 anti-CD56 (MY31;BD) ...
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An innovative hematopoietic stem cell gene therapy approach benefits CLN1 disease in the mouse modelbioRxiv - Neuroscience 2022Quote: ... mouse Alexa647 anti-human human CD271 (NGF Receptor) (BD Pharmingen). For characterization of lin-purity ...
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bioRxiv - Biochemistry 2021Quote: ... Human CD45 (BD #) chimerism in these hematopoietic tissues was analyzed by Accuri C6 flow cytometry.
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bioRxiv - Immunology 2022Quote: ... cells counted under the microscope and stained in FACS buffer (PBS, 2% FCS, 2mM EDTA) with purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™, BD Catalog #553141) and the specific antibody cocktail ...
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bioRxiv - Immunology 2021Quote: ... Disease burden was monitored in the blood by CD45+CD5+CD19+ frequency on an LSR II flow cytometer (BD Biosciences) and CD45+ cell count on a MACSQuant VYB Flow Cytometer (Miltenyi Biotec) ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were incubated with a lineage antibody cocktail (B220, CD3, CD4, CD8, Gr1, CD11b, NK1.1, Il7R, CD19) and were sorted on FACSAria II sorter (BD Biosciences) to recover GMPs ...
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bioRxiv - Immunology 2019Quote: ... FCRL6+/FCRL6- pro B (AA4.1+Lin-CD43+B220+CD19-IgM-) cells from the FL and BM were sorted using a FACSAria II sorter (BD).
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bioRxiv - Pathology 2021Quote: Memory B cells were isolated from peripheral blood mononuclear cells (PBMCs) through magnetic cell sorting with 0.5 μg/ml anti-CD19-PECy7 antibodies (BD, 341113) and mouse anti-PE microbeads (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... fentanyl-reactive B cells were defined as live fentanyl+decoy-CD19+ and sorted into 384-well plates containing lysis buffer using an ARIA II cell sorter (BD Bioscience and using the index-sort function).
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The transcription factor reservoir and chromatin landscape in activated plasmacytoid dendritic cellsbioRxiv - Molecular Biology 2021Quote: ... For ATAC-Seq BM-derived pDCs (CD3-CD19-CD11c+CD11blowB220+SiglecH+CD317+) were FACS purified using FACS Aria III (BD). The pDCs were left untreated or stimulated with 1μM CpG 2216 complexed to transfection reagent DOTAP (Roche ...
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bioRxiv - Cancer Biology 2022Quote: ... 6,000 CD45+CD19-TCRβ+ cells (i.e., T cells), and 20,000 CD45+CD19-TCRβ- cells (e.g., neutrophils) using FACS Aria (BD Bioscience, NJ, USA). The obtained mixture of cells was subjected to single-cell transcriptomics.
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bioRxiv - Genetics 2022Quote: ... lymph node and thymus of Myce and littermate WT control were harvested into single-cell suspension with red cell lysis and stained with CD19-BUV395 (1D3, BD), TCRβ-PE (H57-597 ...
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bioRxiv - Immunology 2023Quote: ... DCs were then isolated via flow sorting (CD3-CD19- CD45R-Ly6G-MerTK-NK1.1-Siglec-F-Ter119-, CD45+CD11c+IA/IE+) using an Influx (BD BioSciences) to a purity of 95-99% ...
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bioRxiv - Immunology 2023Quote: ... The wells were blocked with 1% BSA/PBS before addition of IgA B cells (CD3- CD14-CD19+IgD-IgA+CD27+/-CD38+/-) that had been bulk-sorted from stained PBMCs using a FACSAriaIII instrument (BD). The cells were incubated overnight at 37°C ...
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bioRxiv - Immunology 2021Quote: ... cells were blocked with mouse Fc block (clone: 2.4G2; BD Biosciences) for 10 min followed by staining with fluorescent dye conjugated antibodies specific to surface markers for 20 min at 4°C in the dark ...
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bioRxiv - Immunology 2020Quote: ... followed by Fc receptor blocking using CD16/32 antibody (BD Biosciences). Intracellular stainings were realized using eBioscience Foxp3 / Transcription Factor Staining Buffer Set except for MHC II-CLIP peptide staining where cells were fixed using 4% PFA before permeabilization and staining using a 0.1% saponine ...
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bioRxiv - Immunology 2020Quote: ... cells were incubated with anti-Fc receptor (clone 2.4G2, BD Biosciences) for 7-12 minutes ...
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bioRxiv - Neuroscience 2022Quote: Cells pellets were incubated with Mouse Fc block (BD Biosciences 553142), Fixable Viability Stain 620 (BD Biosciences 564996 ...
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bioRxiv - Immunology 2019Quote: Fc receptors were blocked with anti-CD16/32 (2.4G2, BD Pharmingen). Cell viability was assessed using Zombie Violet dye (Biolegend) ...
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bioRxiv - Immunology 2021Quote: ... followed by Fc-receptor blockade with anti-CD16/CD32 (553141, BD) for mouse cells or Human TruStain FcX (422301 ...
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bioRxiv - Immunology 2021Quote: ... Cells were first incubated with CD16/CD32 Fc block (BD Biosciences), followed by a cell viability stain (either Live/Dead Fixable Aqua Dead Cell Stain Kit ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were blocked with anti-CD16/32 (FC block, BD Pharmigen) for 20 minutes and then incubated with the following antibodies for 30 minutes on ice ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: All flow cytometry FCS files were analyzed with FlowJo (v10, BD). Statistical analyses were performed with GraphPad Prism 9 ...
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bioRxiv - Microbiology 2022Quote: ... Single cell suspensions were preincubated with Fc Block antibody (BD PharMingen) in PBS + 2% FBS for 10 min at room temperature before staining ...
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bioRxiv - Developmental Biology 2022Quote: ... All data were analysed with FCS Express 6 software (BD Biosciences).
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bioRxiv - Microbiology 2022Quote: ... Single cell suspensions were preincubated with Fc Block antibody (BD PharMingen) in PBS + 2% heat-inactivated FBS for 10 min at room temperature before staining ...
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bioRxiv - Cell Biology 2022Quote: ... unfractionated BM cells were incubated with Fc block (BD Pharmigen 553141), followed by biotin-conjugated anti-lineage marker antibodies (CD4 (Biolegend 553649) ...
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bioRxiv - Immunology 2019Quote: ... purified rat anti-mouse CD16/CD32 antibody (Mouse BD Fc Block) (BD Biosciences ...
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bioRxiv - Cancer Biology 2020Quote: ... We then manually gated the individual FCS files using FlowJo (BD) according to the gating scheme described in supplementary material ...
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bioRxiv - Microbiology 2020Quote: Cells were pre-incubated with CD16/CD32 Fc block (BD PharMingen). Surface and intracellular cytokine staining was performed per manufacturer’s instructions in PBS + 2% FBS for 20 min on ice ...
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bioRxiv - Immunology 2019Quote: ... were counted and blocked with anti-mouse FC-block (BD Biosciences) per manufacturer’s protocol for 15 minutes on ice ...
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bioRxiv - Molecular Biology 2019Quote: ... Fc receptors were blocked with CD16/CD32 (clone 2.4G2, BD Biosciences). Cells (1×107 ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were treated with FC Block (BD, San Diego, CA, USA) and labeled with antibodies against CD31 (FAB3628N ...
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bioRxiv - Immunology 2021Quote: ... Samples were incubated with mouse Fc Block (BD Biosciences, catalogue# 553141) for 10 minutes at 4°C and either or both mouse anti-PE and anti-APC were added at 0.5μg/100μL depending on the pMHC multimer conjugates used ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 µL of CD16/CD32 Fc Block antibody (BD Biosciences 553141) was added for 10 minutes at 4°C in the dark ...