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Citations for Becton, Dickinson and Company :
551 - 600 of 1673 citations for 6 Pteridinemethanol 2 amino 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... permeabilized with FACS Permeabilization Buffer 2 (BD Biosciences), and stained for intracellular markers with V450 anti-IFN-D (BD Biosciences ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were rinsed with 2 × 2 ml Pharmingen stain buffer and fixed with 4.21% (w/w) formaldehyde (BD Biosciences, #554655). Cells were analyzed on LSRFortessa X-20 (BD Biosciences ...
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bioRxiv - Microbiology 2020Quote: ... albicans MTL a/α clinical isolate SC5314 72 was used for all experiments and was routinely grown in YPD (1% yeast extract, 2% peptone, 2% glucose; all BD Biosciences) at 30 °C.
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bioRxiv - Immunology 2021Quote: ... To this end 1×104 targets per well and 2×104 effector cells per well were each preincubated for 2 h in 10 μg/ml anti-human CD178 antibodies (NOK-1 and NOK-2, BD Biosciences) or 5 μg/ml anti-human TRAIL antibody (RIK-2 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed with ice-cold FACS buffer (DPBS supplemented with 2% FBS and 2 mM EDTA) and subjected to fixation and staining with Cell Fixation and Permeabilization Kits (BD Biosciences). Incubation with antibodies was performed for 1h at RT ...
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bioRxiv - Immunology 2023Quote: ... All flow cytometry analyses were performed in FACS buffer containing PBS with 2 mM EDTA and 2% FBS on a FACS CANTO (BD Pharmingen) and analysed with FlowJo software (Treestar).
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bioRxiv - Cancer Biology 2023Quote: ... CD49fhighEpCAMlowLin- cells from 2-month-old C57BL/6 WT mice were sorted using FACS and resuspended in culture media (DMEM/F12+2% FBS+1% PSA+2% B27) supplemented with EGF (10 ng/ mL, BD Bioscience), Rspo1(250 ng/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... CD49fhighEpCAMlowLin- cells were collected from FACS and cultured in 200 μL culture media (DMEM/F12+2% FBS+1% PSA+2% B27) supplemented with 10 ng/ mL EGF (BD Bioscience), 250 ng/ mL Rspo1(R&D) ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were blocked with 2% ECL prime blocking reagent in PBS-T (Amersham; #RPN418) and incubated against AP-2 (mouse-anti-AP50, BD, #A611351) and GAPDH (rabbit-anti-GAPDH ...
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bioRxiv - Immunology 2023Quote: ... Samples were stored in PBS containing 2% (V/V) FBS and 2 mM EDTA until acquisition on an LSR Fortessa flow cytometer (BD Biosciences). Analyses were performed with FlowJo v10.7.1 (BD Biosciences).
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bioRxiv - Microbiology 2023Quote: ... and values for IL-2 were determined by using a standard curve generated by using known concentrations of purified recombinant murine IL-2 (BD Biosciences).
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bioRxiv - Immunology 2023Quote: ... up to 2 x106 MR1-5-OP-RU-APC Tetramer magnetic bead enriched spleen (n= 2) or unenriched colon (n= 2) single cell suspensions were labeled with an oligonucleotide-tagged antibody sample tag (BD Biosciences). MAIT cells (MR1-5-OP-RU-Tet+TCRβ+CD3+ ...
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bioRxiv - Biophysics 2023Quote: ... Cells were then washed twice in FACS buffer (PBS, 2% FBS, 2 mM EDTA) and permeabilized in BD Phosflow Perm Buffer III (BD Biosciences), according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... single cell suspension of electroporated iPS cells was plated on Matrigel-coated 6 well plates (WP) (BD Bioscience #351146). Once cultures were adherent and recovered to ∼80% confluency ...
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bioRxiv - Immunology 2022Quote: ... CD25-) were sorted from C57BL/6 background WT or Rag1-/- mice using a FACSaria Fusion cell sorter (BD Biosciences), and 3 x 104 sorted cells were added to each well containing irradiated feeder cells ...
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bioRxiv - Physiology 2022Quote: Primary mouse hepatocytes were cultured in BD BioCoat™Collagen 6-well plates (BD Pharmingen, Franklin Lakes, NJ, USA) at 1-1.5 x 106 cells per well in William’s medium (catalog n°12551032 ...
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bioRxiv - Neuroscience 2019Quote: ... the transfected cell suspension was diluted in a 1:6 – 1:8 ratio and plated into Matrigel (BD Biosciences)-coated wells of 6-well plates ...
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bioRxiv - Genomics 2019Quote: ... permeabilization and intracellular staining for Ikaros and BCL-6 was performed using the Transcription Factor Buffer Set (BD Pharmingen). Cells were analyzed on a CytoFLEX flow cytometer (Beckman Coulter).
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bioRxiv - Immunology 2021Quote: ... Approximately 6% of the corneal cells were analyzed using the BD LSR II cytometer (BD Biosciences, San Jose, CA) and flow data were processed using the FlowJo software version 10.6.2 (Tree Star ...
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bioRxiv - Immunology 2021Quote: ... cells were re-stimulated with peptides for 6 hours in the presence of Leuko Act Cktl with GolgiPlug (BD) plus 50 IU/mL IL-2 ...
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bioRxiv - Cancer Biology 2019Quote: ... Data was acquired using a BD LSRFortessa™ and analyzed using BD FACSDiva™ Software version 6 (BD Biosciences).
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bioRxiv - Cancer Biology 2020Quote: ... cells were washed and the following antibodies were used for the second round (volume per 1×10^6 cells): CD45 V500 (4μl, BD), CD34 APC-Cy7 (3μl ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 20 batches of 24 eggs each were placed in wells of 6-well plates (Falcon, BD Biosciences, Allschwil, Switzerland). Ten mL of diluted milt of 2 males each was prepared such that each male was represented with the same concentration of active sperm (25 Mio/mL ...
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bioRxiv - Immunology 2022Quote: ... MCP-1 and IL-6 cytokine levels were quantified using the Cytometric bead assay Mouse inflammation kit (BD Bioscience) as recommended by the manufacturer and measured by flow cytometry using a FACS Fortessa flow cytometer.
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bioRxiv - Immunology 2022Quote: ... Cells were stimulated for 4–6 h with 0.5 μM CpG or 50 ng/ml PMA/1 mM ionomycin with GolgiStop (BD). Then ...
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bioRxiv - Systems Biology 2022Quote: ... 1 × 10^6 Cells were stained with PE anti-human CD11b antibodies (20 μL per test, BD Pharmingen, USA) and APC-Cy7 anti-human CD14 antibodies (5 μL per test ...
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bioRxiv - Biochemistry 2019Quote: ... IL-6 levels in the supernatant were quantified using respective human enzyme-linked immunosorbent assay (ELISA) kits (BD OptEIA) as per the manufacturer’s protocol.
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bioRxiv - Microbiology 2020Quote: The inflammatory cytokine response (IL-1β, IL-6, and TNF-α) was determined using Cytometric Bead Array (BD Biosciences) per the manufacturer’s instructions.
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bioRxiv - Microbiology 2019Quote: ... LB agar (2.5 ml) was added to the wells of 6-well plates (BD Biosciences Inc., Franklin Lakes, NJ) and allowed to solidify ...
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bioRxiv - Cell Biology 2019Quote: Melan-a cells were trypsinized and seeded at density of 1X105 cells/well in 6 well plate (BD Bioscience) and incubated overnight with antibiotic containing RPMI 1640+10% FBS ...
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bioRxiv - Cell Biology 2020Quote: Antibody for surface staining and uptake experiments: mouse anti-GFP-Alexa fluor 647 (BD Biosciences clone 1A12-6-18)
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bioRxiv - Developmental Biology 2021Quote: ... from E16.5 C57BL/6 lungs pooled by litter and cultured on a layer of growth factor reduced Matrigel (BD) in serum-free media (DMEM/F12 supplemented with L-glutamine and penicillin-streptomycin ...
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bioRxiv - Immunology 2020Quote: ... for 16 h before the quantification of IL-12p40 and IL-6 in the supernatant by ELISA (BD Biosciences). For phenotypic analysis (MHC II ...
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bioRxiv - Microbiology 2020Quote: ... hESCs were maintained in feeder-free culture conditions in 6-well tissue culture dishes on Matrigel (BD Biosciences, 354277) in mTeSR1 medium (Stem Cell Technologies ...
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bioRxiv - Immunology 2021Quote: ... cells were re-stimulated with peptides for 6 hours in the presence of GolgiPlug and GolgiStop (BD Cat#550583) plus 50 IU/mL IL-2 ...
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bioRxiv - Immunology 2021Quote: ... cells were resuspended in enzyme-free ethylenediaminetetraacetic acid (EDTA) buffer and replated in untreated 6-well plates (BD Falcon) at 1 M cells per well in unconditioned medium ±25 U/ml IFN-γ (Peprotech ...
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bioRxiv - Biochemistry 2024Quote: ... PFB-FDGlu (50 µg/mL) was added for 6 h and the nPFB-FDGlu fluorescence was measured by BD FACSAria™ III Cell Sorter (λex 492 nm and λem 516 nm) ...
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bioRxiv - Biochemistry 2024Quote: ... PFB-FDGlu (50 µg/mL) was added for 6 h and the nPFB-FDGlu fluorescence was measured by BD FACSAria™ III Cell Sorter (λex 492 nm and λem 516 nm).
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bioRxiv - Biochemistry 2023Quote: ... IBG3 (0.1 nM) or IBG4 (100 nM) for 6 hours before flow cytometry analysis on an LSRFortessa (BD Biosciences).
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bioRxiv - Immunology 2023Quote: Blood from indicated mouse strains was harvested via heart puncture and centrifuged at 10,000 g for 6 minutes in serum separation tubes (BD Microtainer Blood Collection Tubes Cat ...
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bioRxiv - Immunology 2023Quote: Mice were injected (i.p) either at day 1 or day 6 post-infection with 1mg of BrdU (BD Biosciences); 48h later ...
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bioRxiv - Immunology 2023Quote: ... plates were washed three times again followed by incubation with biotinylated anti-IL-6 (1:500, BD Biosciences, #554402) and anti-TNF antibodies (1:250 ...
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bioRxiv - Immunology 2023Quote: ... plates were washed three times again followed by incubation with biotinylated anti-IL-6 (1:500, BD Biosciences, #554402) and anti-TNF (1:250 ...
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bioRxiv - Cell Biology 2024Quote: ... the levels of secreted cytokines were quantified using the BD OptEIA™ Human IL-6 ELISA kit (BD Biosciences). Cells were trypsinized and counted for normalization of cytokine levels per cell.
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bioRxiv - Cell Biology 2022Quote: ... in SIH defined minimal medium (Formedium, Norfolk, England) or on SM/5 agar (2 g glucose, 2 g bactopeptone (BD, Sparks, MD), 0.2 g yeast extract (Hardy Diagnostics ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: SARS-CoV-2 infected cells were fixed in 2% formaldehyde for 30 mins and permeabilized with BD Perm/Wash buffer (BD Biosciences, 554723). The primary antibody used was sheep anti-SARS-CoV-2 nucleocapsid antibody (DA114 ...
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bioRxiv - Immunology 2021Quote: ... 50 U/ml Penicillin and 50 ug/ml Streptomycin) supplemented with 100 U/ml hIL-2 and 2 ug/ml anti-CD28 (BD Pharmingen, 553294). Two days after the primary stimulation ...
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bioRxiv - Immunology 2023Quote: ... were diluted 1:50 in PBS-10% FBS and IL-2 concentration was determined by ELISA assays using the OptEIA Human IL-2 ELISA commercial kit (BD Biosciences, USA) and revealed with TMB substrate (Thermofisher ...
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bioRxiv - Genetics 2024Quote: ... After 24 hours we selected the transfected cells either by puromycin treatment (2 ug/ml for 2 days) or flowcytometric sorting for double positive cells (BD FACSAriaTM fusion).
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bioRxiv - Cell Biology 2020Quote: ... 2 mL of dispase (BD Biosciences, 50 U/ml) was instilled into the lungs through the trachea until the lungs inflated ...