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Citations for Becton, Dickinson and Company :
5701 - 5750 of 5857 citations for Mouse Switch associated protein 70 SWAP70 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... and cells were fixed and permeabilized at room temperature for 15 min with Fixation/Permeabilization Solution Kit (BD Biosciences; cat. #554714). For each sample a minimum of 1.2x105 stopping gate events (live CD3+ T-cells ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were collected at 72 h post FeNPs administration and detected with the Annexin V-FITC Apoptosis Detection Kit (BD, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... GMPGFP+ and GMPGFP- or clonal HoxA9 cells were sorted directly in the fixation buffer from the Fixation/Permeabilization kit (BD Biosciences). Cells were then washed with PBS supplemented with 3% BSA followed by the Click-iT reaction as described above ...
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bioRxiv - Cell Biology 2020Quote: ... intracellular staining with anti-FoxP3-FITC antibodies (clone: FJK-16s; Thermo Fischer Scientific) was performed with the BD Cytofix/Cytoperm Kit (BD Biosciences) according to the manufacture instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... treated with 75µM cisplatin and stained with Annexin V conjugated to Alexa Fluor 488 dye and propidium iodide (PI) using Dead Cell Apoptosis Kit (BD Biosciences) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... fixable viability dye was included with the surface staining antibodies (20 minutes, RT) and intracellular staining used the Cytofix/Cytoperm kit (BD Biosciences) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... TNF-α and IFN-γ in the serum of experimental monkeys were detected with the Non-Human Primate (NHP) Th1/Th2 Cytokine Kit (BD) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... The enzyme activity was stopped with fetal bovine serum (FBS) followed by the fixation and staining with the APC BrDU Flow Kit – Part A (552598, BD Pharmingen) according to the manufacturer’s recommendation with some exceptions ...
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bioRxiv - Immunology 2022Quote: ... Intracellular staining was performed after treatment with Fixation/Permeabilization solution and in the presence of Perm/Wash buffer (Cytofix/ Cytoperm kit, BD Biosciences) using anti-mouse IFN-γ FITC ...
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bioRxiv - Immunology 2022Quote: ... The Foxp3 staining kit was used for intracellular transcription factor staining and the Cytoperm/Cytofix Plus staining kit was used for cytokine staining (BD Biosciences). Data was analyzed using FlowJo (TreeStar) ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed twice with 500 μL permeabilization buffer (from kit described above) and stained with 150 μL permeabilization buffer containing 1:400 Ki67-Alexa-Fluor antibody (561126, BD Biosciences) for 30 minutes at 4 °C ...
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bioRxiv - Immunology 2019Quote: ... Intracellular cytokine staining was performed to examine the production of cytokines in these CAR-T cells using Fixation/Permeabilization Solution Kit (BD Biosciences). CD107 expression was determined as described (Liu et al. ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... IL-12 and IL-6 was simultaneously analyzed by flow cytometry (FACS Canto II; Becton-Dickinson) using the BD Cytometric Bead Array (CBA) Human Inflammatory Cytokines Kit (BD™) according recommendations ...
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bioRxiv - Immunology 2019Quote: Cell surface staining was performed as described in the protocol “Single Cell Labelling with the BD Single-Cell Multiplexing Kit and BD AbSeq Ab-oligos” (BD Biosciences). Briefly ...
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bioRxiv - Genetics 2020Quote: ... and permeabilized using BD Cytofix/Cytoperm kit before incubation with BV605 anti-human IFN-gamma (B27 clone) and BUV395 anti-human TNF-alfa (MAB11 clone, BD Biosciences) in permeabilization buffer at 4°C for 30 min in the dark ...
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bioRxiv - Cancer Biology 2019Quote: ... NODAL and CA9 staining was performed on fixed and permeabilized cells according to manufacturer instructions using the Fixation/Permeabilization kit (BD Biosciences).
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bioRxiv - Cancer Biology 2019Quote: Exponentially growing cells in six-well plates were treated with 3 μM BAY-876 or DMSO for 24h before cell cycle analysis using allophycocyanin (APC) BrdU Flow kit (BD Pharmingen). Briefly ...
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bioRxiv - Cancer Biology 2020Quote: Cells were double stained with propidium iodide (PI) and Annexin V by using FITC Annexin V apoptosis detection kit (BD Biosciences) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: ... were sorted directly into four 96 well plates « Precise WTA Single Cell Encoding Plate » (BDTM Precise WTA Single Cell Kit, BD Genomics) (1 cell/well ...
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bioRxiv - Immunology 2020Quote: ... Total CD4+ T cells were isolated by negative magnetic selection using a CD4 T cell isolation kit (Stemcell) and CD4+CD25hiCD127lo-neg Treg cells were sorted on a FACS Aria (BD Biosciences). Treg cells were cultured in RPMI 1640 medium supplemented with 5% Human serum ...
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bioRxiv - Cell Biology 2019Quote: ... Phenotypic characterization of each lot was done by flow cytometry using the BD StemflowTM hMSC Analysis kit and the endothelial cell marker CD31 (all reagents from BD Bioscience) and showed that the 2 lots of hUC-MSC met the ISCT phenotypic criteria for MSCs (>95% positive for CD73 ...
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bioRxiv - Genetics 2021Quote: ... at least 2x106 iPSC-PPC cells were fixed and permeabilized using the Fixation/Permeabilization Solution Kit with BD GolgiStopTM (BD Biosciences) following manufacturer recommendations ...
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bioRxiv - Bioengineering 2021Quote: ... for assays which required non-transcription factor internal staining (cytokines alone) fixation and permeabilization was performed using the Cytofix/Cytoperm kit (BD Biosciences) according to manufacturer instructions ...
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bioRxiv - Cell Biology 2020Quote: The following monoclonal antibodies (Abs) were used for flow cytometry and cell sorting: anti-c-Kit (2B8, BD Biosciences, 1:100), -Sca-1 (E13-161.7 ...
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bioRxiv - Pathology 2021Quote: ... CD11b) and Sca1+/c-Kit+ cells in lineage negative cells determined via flow cytometry on a BD LSRII instrument (BD Biosciences). Data was analyzed on FlowJo software (Treestar) ...
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bioRxiv - Immunology 2021Quote: ... 4 and 6 hours and cytokines detected by intracellular cytokine staining (see Table S2 for antibodies list) using Fixation/Permeabilization Solution Kit (BD Biosciences), unstimulated cells were used as a control.
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bioRxiv - Immunology 2020Quote: ... stimulation of single cell suspensions in the presence of GolgiPlug for 4 hours prior to intracellular staining using BD Cytofix/Cytoperm kit (BD Biosciences). Viability was determined using Fixable Viability Dye eFluor 780 (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: ... either cell supernatants or cell extracts were used for cytokine estimation with specific kits- TNFα (cat no: 88-7346-77, eBioscience) and IL1β (cat no: 557953, BD Biosciences) ELISA kits according to manufacturer’s protocol.
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bioRxiv - Microbiology 2019Quote: ... we diluted test cultures 100-fold and stained with thiazole orange and propidium iodide (BD Cell Viability Kit, product no. 349483). In parallel ...
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bioRxiv - Immunology 2020Quote: ... Spleen B cells were analyzed for BrdU incorporation (by anti-BrdU mAb staining) and DNA contents (by 7–AAD staining after cells were permeabilized) using the APC BrdU Flow kit® (BD) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... and intracellular staining was performed after the fixation and permeabilization of the cells (intracellular staining kit from ThermoFisher or BD Biosciences). Analysis of cells collected from in vivo experiments were performed following Fc block (2.4G2 ...
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bioRxiv - Immunology 2020Quote: ... We only used iTreg cells for experiments if samples achieved at least 80% Foxp3 positive cells (00552300, Foxp3 Staining Kit, BD Bioscience).
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bioRxiv - Immunology 2021Quote: ... Spike -specific T cells were measured by surface staining followed by fixation and permeabilization using BD fixation/permeabilization solution kit (BD Biosciences,) and intracellular cytokine staining (table S1) ...
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bioRxiv - Immunology 2021Quote: ... splenocytes were isolated and immunolabeled with anti-CD4 and anti-Foxp3 antibodies and BrdU incorporation was analyzed by a BrdU Flow kit per the manufacturer’s protocol (BD Pharmingen, 552598) (15).
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bioRxiv - Immunology 2022Quote: ... cells were cultured in the presence of 1 μg/mL brefeldin A and monensin (00-4505-51, eBioscience) for 2 hr and were then fixed and permeabilized with Cytofix/Cytoperm kit (BD Biosciences) according to manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... Libraries were then prepared using the BD Rhapsody WTA Amplification Kit (#633801) following instructions of the mRNA WTA Library Preparation Protocol (BD Biosciences). For the MULTI-seq library first the BD protocol of Sample Tag Library Preparation was followed until purification of Sample Tag PCR1 product with the difference of adding the MULTI-seq primer (sequence according to McGinnis and Patterson et al.5 ...
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bioRxiv - Immunology 2022Quote: RFP+GFP+ FOXP3+ Tregs and RFP+GFP- exFOXP3 T cells isolated from spleen and CNS from EAE mice were stained with sample tag antibodies (Ms Single Cell Sampling Multiplexing Kit, BD Biosciences). Next ...
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bioRxiv - Immunology 2022Quote: ... and cells were fixed and permeabilized at room temperature for 15 min with Fixation/Permeabilization Solution Kit (BD Biosciences; cat. #554714). For each sample a minimum of 1.2×105 stopping gate events (live CD3+ T-cells ...
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“Identification of microRNAs regulated by E2F transcription factors in human pluripotent stem cells”bioRxiv - Developmental Biology 2024Quote: The distribution of cell populations throughout the cell cycle and the fraction of cells capable of incorporating BrdU were determined using the BrdU Flow Kit (BD Biosciences). After treatments cells were incubated with BrdU (10 ⎧M ...
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bioRxiv - Neuroscience 2022Quote: ... and tumor necrosis factor alpha (TNFα) in cell supernatants were measured using the Human Inflammatory Cytokine Cytometric Bead Array Kit (BD Biosciences). A LEGENDplex™ HSC Myeloid Panel kit (Biolegend ...
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bioRxiv - Bioengineering 2024Quote: ... and the hemoglobin contents were quantified after 8 h by the colorimetric assay kit (absorbance: 550 nm; with BD Pharm Lyse). Each value was subtracted from the internal control without the anti-BSA antibody.
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bioRxiv - Neuroscience 2024Quote: The rate of apoptosis induced by microRNA-29a-5p mimics and inhibitors transfection in astrocytes after OGD/R was determined using Annexin V/PI kit (BD Biosciences). Briefly ...
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bioRxiv - Genomics 2024Quote: ... at least 2 x 106 cells were fixed and permeabilized using the Fixation/Permeabilized Solution Kit with BD GolgiStop TM (BD Biosciences) following the manufacturer’s recommendations ...
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bioRxiv - Immunology 2024Quote: ... Cell apoptosis was measured by co-staining cells with Annexin V and 7-AAD using the PE Annexin V Apoptosis Detection Kit I (BD Biosciences), according to manufacturer’s protocol.
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed with ice-cold FACS buffer (DPBS supplemented with 2% FBS and 2 mM EDTA) and subjected to fixation and staining with Cell Fixation and Permeabilization Kits (BD Biosciences). Incubation with antibodies was performed for 1h at RT ...
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bioRxiv - Immunology 2024Quote: ... and CD45.2+ peripheral blood leukocytes were FACS-sorted and labeled using the BD Rhapsody Single-Cell Multiplexing CTT Kit (BD Biosciences, 626545). On average ...
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bioRxiv - Cell Biology 2023Quote: ... Post incubation cells were processed for apoptosis using flow cytometry according to the protocol described in FITC Annexin-V apoptosis detection kit (BD Pharmingen).
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bioRxiv - Genetics 2023Quote: ... 1.106 cells were incubated 20 min at 37°C in 100 μL solution BD cytofix/ cytoperm (BD PharmingenTM BrdU Flow Kit, 552598, BD Bioscience), washed with PBS and re-suspended in 700 µl of staining buffer (PBS with 3% SVF) ...
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bioRxiv - Immunology 2023Quote: ... and ionomycin (500 ng/mL) for 3 hrs at 37°C prior to surface and intracellular staining using the BD Cytofix/Cytoperm Kit (BD Biosciences). Intracellular transcription factor staining was performed using a Foxp3 staining kit (ThermoFisher) ...
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bioRxiv - Developmental Biology 2023Quote: ... The final cDNA library was generated from double strand full length cDNA by random priming amplification with the BD Rhapsody cDNA Kit (633773, BD Biosciences) and the BD Rhapsody Targeted mRNA & AbSeq Amplification Kit (633774 ...