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Citations for Becton, Dickinson and Company :
5601 - 5650 of 6484 citations for Mouse Bcl 2 Modifying Factor BMF ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... and the BD Rhapsody Targeted mRNA & AbSeq Amplification Kit (633774, BD Biosciences). All the libraries were sequenced in a PE150 mode (Pair-End for 150bp read ...
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bioRxiv - Immunology 2023Quote: ... Note these experiments were carried out using early access kits from BD Genomics before the implementation of commercially-available single-cell protein/RNA assays (e.g ...
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Autophagy suppression in DNA damaged cells occurs through a newly identified p53-proteasome-LC3 axisbioRxiv - Cell Biology 2024Quote: ... Cells were stained using Annexin-V PI kit (#556547, BD Pharmingen, USA) as described in the kit manufacturer’s protocol.
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bioRxiv - Immunology 2024Quote: ... and processed with BD Cytofix/Cytoperm Fixation and Permeabilization Kit (BD 554714). Anti-H3K27ac primary antibody (Sigma SAB4200485 ...
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bioRxiv - Immunology 2024Quote: ... was purchased from BD Biosciences and cells were fixed and permeabilized according to kit instructions (BD Biosciences Cytofix/Cytoperm Cat ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were labelled with the PE Annexin V Apoptosis kit (BD Biosciences) to distinguish between early and late apoptosis by flow-cytometry ...
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bioRxiv - Immunology 2024Quote: ... of cells labelled with APC-coupled anti-CD117 (c-kit) (BD Biosciences), from Lin- BM samples 6- to −10-week-old C57BL/6 mice (The Jackson Laboratory ...
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bioRxiv - Immunology 2024Quote: ... samples were fixed and permeabilized with a Cytofix/Cytoperm kit (BD Biosciences) for 20 min at 4 LC ...
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bioRxiv - Molecular Biology 2024Quote: ... on a single lane cartridge following manufacturer instructions (BD Rhapsody WTA kit). Subsequently ...
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bioRxiv - Immunology 2024Quote: ... fixed and permeabilized using the BD Cytofix/Cytoperm™ kit (BD Biosciences). Cells were washed and then stained with anti-IgM Fab and anti-IgG Fab along with anti-rabbit AF488-labeled secondary antibody (Table 2) ...
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bioRxiv - Immunology 2024Quote: ... Cytokine staining was performed using the BD Cytofix/Cytoperm Kit (BD Biosciences). Flow cytometry analysis was performed on a BD LSRFortessa X-20 and BD FACSymphony A3 Cell Analysers (BD Biosciences ...
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bioRxiv - Molecular Biology 2024Quote: ... fixed and permeabilized using reagents from the CytoFix/CytoPerm Kit (BD Biosciences) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Single-cell cDNA synthesis was performed using BD Rhapsody cDNA Kit (BD) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... The bottom layer of soft agar was prepared by mixing 2 × growth medium and 1.5% noble agar (BD Biosciences, San Jose, CA) at a 1:1 ratio and the mixture was poured into 6-well plates ...
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bioRxiv - Cell Biology 2020Quote: ... resuspended in buffer solution (PBS + 2% FBS + 2mM EDTA) and blocked with recombinant Fc protein (1:100 dilution, BD Biosciences, cat#553142). Cells were then incubated with the primary antibody solution (1:100 dilution ...
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Planarian CREB-binding protein (CBP) gene family regulates stem cell maintenance and differentiationbioRxiv - Developmental Biology 2020Quote: ... which was incubated for 2 h in the dark at 37°C with 20 µM caspase-3 substrate Ac-DEVD-AMC (BD Biosciences Pharmingen) or 2 ml from a stock of 1 mg/ml for a final volume of 150 µl ...
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bioRxiv - Developmental Biology 2019Quote: ... Passages were performed by resuspending Matrigel-embedded organoids in cold DPBS and transferring them to a Falcon tube using a 2 mL syringe with a 27 G ½ needle (BD Microlance #300635) to break them up ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were washed with 1x Permeabilization buffer (eBioscience) and incubated for 15 min in FACS buffer (PBS, 2% FCS) before adding the primary MASH-1 antibody (BD Biosciences, 556604), 1:250 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 4 μL of the saturated culture were transferred in 60 uL PBS and the samples were stored at 4 °C for 1-2 days before being assayed by flow cytometry (BD FACSCanto II). Data were analyzed in FlowJo ...
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bioRxiv - Bioengineering 2021Quote: ... albicans fungal cells (CMM16) were inoculated from a streaked plate in 2 ml yeast extract peptone dextrose (YPD) [1% yeast extract (BD Biosciences, 212730), 2% peptone (BD Biosciences ...
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bioRxiv - Molecular Biology 2021Quote: ... Between 2 - 4x105 events were collected per sample within 3 hours after staining on an LSRFortessa (BD Biosciences, 4 lasers, 18 parameters) and analyzed using FlowJo software (version 9.7.4 ...
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bioRxiv - Microbiology 2020Quote: ... Strains were precultured during 48h in 300 μL of Synthetic Defined (SD) medium (Warringer et al., 2011) with some modifications (2% glucose, 0.14% Yeast Nitrogen Base (BD Difco™, U.S.A.), 0.5% ammonium sulfate ...
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bioRxiv - Microbiology 2021Quote: ... the ELISA was performed by coating 96 well plates with 2 µg/mL LRRC15-His (AcroBio, #LR5-H52H3) in coating buffer (BD, #51-2713KC) overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: Streptococcus pneumoniae D39 (serotype 2 clinical isolate) and isogenic mutant strains were cultured in Todd-Hewitt broth (Becton, Dickinson and Company; BD) supplemented with 0.2% yeast extract (BD ...
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bioRxiv - Microbiology 2020Quote: Streptococcus pneumoniae D39 (serotype 2 clinical isolate) and isogenic mutant strains were cultured in Todd-Hewitt broth (Becton, Dickinson and Company; BD) supplemented with 0.2% yeast extract (BD ...
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bioRxiv - Microbiology 2020Quote: ... 50 μL of EDTA-anticoagulated whole blood were stained with the monoclonal antibodies CD3 BV605 (SP34-2, BD Biosciences, San Jose, CA), CD4 PerCP/Cyanine5.5 (Biolegend ...
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bioRxiv - Cancer Biology 2020Quote: ... then probed with the following primary antibodies in 5% milk/TBS-T overnight at 4 °C: SREBP-2 (1:250; BD Biosciences, 557037), p44/42 MAPK (ERK1/2 ...
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bioRxiv - Immunology 2022Quote: ... The hemocytes were collected from thirty to forty shrimps at 2 hours post injection for each sorting which was performed on a FACSMelody cell sorter (BD Biosciences, USA). The fluorescence boundary was set based on detection of the shrimp hemocyte self-fluorescence without VP injection.
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bioRxiv - Pathology 2022Quote: ... the samples were washed and resuspended in PBS supplemented with 2% FBS for analysis on a five-laser flow cytometer (BD Biosciences, Fortessa). The data were analysed with FlowJo software (TreeStar).
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bioRxiv - Immunology 2022Quote: ... For stimulation ∼106 lymphocytes plated in medium containing 2% fetal calf serum and 1.5 μl/ml Golgiplug (BD Bioscience; San Jose, CA) were cultured with peptides each present at a final concentration of 2 µg/ml for 5 hr at 37°C in a 5% CO2 incubator ...
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bioRxiv - Immunology 2020Quote: ... 7 and 14) or at 2 time points (days 1and 14) after vaccination into Vacutainer® Heparin tubes (BD Biosciences, Plymouth, U.K.). Blood samples were subjected to immediate preparation and analyses ...
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bioRxiv - Pathology 2020Quote: ... Tomato-positive and -negative cells differed by ∼2 orders of magnitude in tomato fluorescence with excitation of 554nm and emission of 581nm by Influx™ cell sorter (BD Biosciences).
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bioRxiv - Immunology 2019Quote: ... Samples were resuspended to a volume 2.5 mL with PBS with 2% FBS and 0.4% EDTA and placed on an Imag (BD Biosciences, San Diego, CA) for 5 min ...
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bioRxiv - Cell Biology 2019Quote: ... A total of 5x 105 cells resuspended in 20µl PBS were locally injected into the fracture site of bone under anesthesia using a Hamilton syringe (Hamilton, USA) and a 30-gauge 1/2 needle (BD Biosciences, USA). X-rays were taken weekly using a digital X-ray machine (Faxitron X-Ray Corp. ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were then washed with PBS+2% goat serum and cell cycle analysis was conducted using a BD FACS Calibur (BD Biosciences, UK).
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bioRxiv - Cancer Biology 2020Quote: ... two groups of NOD/SCID female mice were orthotopically injected in the mammary fat pad #4 with 2×106 cells of either ALDH1A3 overexpressing or vector control MDA-MB-231 cells (admixed with high concentration Matrigel, BD, Cat # 354262). Mice were euthanized 49 days post cancer cell implantation and the lungs were harvested ...
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bioRxiv - Neuroscience 2020Quote: COs were washed 2 times for 5 minutes at room temperature in 5 mL of PBS in 5 mL round bottom tubes (BD Falcon, #1152367) then fixed overnight in 4% PFA at 4°C on a rocker ...
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bioRxiv - Immunology 2021Quote: ... Macaque cells were incubated with 100 μl of FACS buffer (PBS 1x with 2% fetal bovine serum and 1mM EDTA) with human Fc Block (BD Biosciences #564219) at a 1:500 dilution for 30 min on ice.
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bioRxiv - Genetics 2019Quote: ... in PBS with 1 μg/ml bovine serum albumin (BSA and 2 mM Tris-HCl. Cells were analyzed on a BD LSRII flow cytometer (BD Biosciences, Inc) and data were analyzed using FlowJo software ...
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Mapping of the autophagosomal degradome identifies IL-7Rα as key cargo in proliferating CD4+ T-cellsbioRxiv - Immunology 2021Quote: ... cells were fixed for 10 min at room temperature with 2% Paraformaldehyde and analysed using LSRFortessa™ X-20 Cell Analyzer (BD Biosciences), LSR II Flow Cytometer (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... Lymphocytes were incubated in 200 μl of a mixture of BD FACS™ Permeabilizing Solution 2 and BD FACS™ Lysing Solution prepared according to manufacturer’s instructions (BD Biosciences) for 8 min at RT ...
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bioRxiv - Immunology 2022Quote: ... then fixed with 2% paraformalin and kept on ice until acquisition on a BD Symphony A3 (Becton Dickinson BD; Franklin Lakes, NJ). Data was analyzed using FlowJo 10.8.1 (RRID:SCR_008520 ...
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bioRxiv - Cell Biology 2022Quote: ... Rings were fixed after 7 days with 2% PFA and stained with rat PECAM (dilution 1:100, Rat monoclonal, clone MEC 13.3; BD Biosciences, Cat# 557355). Rings were imaged in 3D using the ZEISS Lightsheet Z.1 microscope ...
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bioRxiv - Microbiology 2022Quote: ... the internal space of the chambers in which biofilms were cultivated measured 5000μm x 500μm x 70μm (LxWxH). Segments of inlet tubing (Cole Parmer PTFE #30, cat. # 06417-11) attached to 27Gx1/2 needles (BD Precision, cat. # 305109) on 1mL syringes (Brandzig ...
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bioRxiv - Physiology 2022Quote: ... Samples were stored at 10-15°C in 2 mL K2-EDTA (ethylenediaminetetraacetic acid) tubes (BD Vacutainer®, Franklin Lakes, NJ, USA) without agitation until analysed 15 to 45 min later.
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bioRxiv - Physiology 2022Quote: ... The sample was stored at 10-12 °C in 2 mL EDTA (ethylenediaminetetraacetic acid) tubes (BD Vacutainer®, Franklin Lakes, NJ, USA) without agitation until analysed 0.5-2.5 h later ...
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bioRxiv - Immunology 2024Quote: ... and staining of surface markers with a mixture of conjugated antibodies 1:250 in 2%FBS PBS 1mM EDTA buffer with 10% Brilliant Stain Buffer Plus (BD, Cat#566385) for 30 min on ice ...
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bioRxiv - Cancer Biology 2024Quote: ... Positively transduced cells were selected with 2 µg/ml puromycin followed by a sterile sort for high GFP and mCherry expression (BD FACSAria II). Expression was maintained with 1 µg/ml puromycin in all experiments except where immune cells were needed.
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bioRxiv - Cell Biology 2024Quote: ... 10 menstrual endometrial fragments (diameter 1-2 mm) from donor animals were inserted using two 14G catheters (Insyte, Vialon, BD, Madrid, Spain) under laparoscopic guidance in both left and right flank and behind the uterus ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then stained for 30min on ice with Alexa-Fluor-488-conjugated mouse monoclonal antibody (mAb) directed against the SARS-CoV-2 spike protein (R&D systems; clone #1035206, diluted 1:50 in Perm/Wash buffer (BD)) ...