Labshake search
Citations for Becton, Dickinson and Company :
501 - 550 of 586 citations for 3 Methyl 3' piperidinomethyl benzophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... The cells followed by an incubation with the SM/Chol probe OlyA (3 µM) and primary anti-IFN-γR1 antibody (1:50) (BD Biosciences) together in 1% BSA-PBS at room temperature for 90 min ...
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bioRxiv - Evolutionary Biology 2023Quote: ... After 48h cultures were diluted and plated on Nutrient Agar plates (5 g/L peptone BD difco, BD Bioscience; 3 g/L yeast extract BD difco, BD Bioscience, 15 g/L agar Bacto, BD Bioscience). Plates were left at room temperature until colonies were detectable and distinguishable (2-4 days) ...
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bioRxiv - Immunology 2024Quote: ... Flow cytometry was performed on a 3 laser Cytek Aurora Cytometer (Cytek Biosciences, Fremont, CA) and analyzed using FlowJo software (BD Biosciences). All antibodies are from Biolegend ...
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bioRxiv - Bioengineering 2024Quote: ... 25,000 irradiated HM-1-luc cells were mixed with 3 × 105 PBMCs per sample and seeded in a 96-well ELISPOT plate (BD Biosciences) that was pre-coated with IFN-γ capture antibody (BD Biosciences) ...
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bioRxiv - Microbiology 2024Quote: ... Cells were activated for 3 days with 1 μg/mL/million cells of purified NA/LE mouse anti-human CD3 and CD28 (BD Biosciences) and infected with HIVBaL at 0.01 ng/106 cells ...
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bioRxiv - Immunology 2024Quote: ... coated plates were blocked with PBS–3% rat serum and incubated with serum samples and with a biotinylated rat antimouse IgE (clone R35–118; BD Biosciences). Avidin-HRP (Ebiosciences ...
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bioRxiv - Immunology 2024Quote: ... cells were stained with AlexaFluor 647-rabbit anti-active caspase-3 antibody (BD, 1:20 in 1X BD Perm/Wash buffer) for 30 minutes at 4°C in the dark ...
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bioRxiv - Neuroscience 2024Quote: ... PNU-120596 or vehicle was back filled into a 1 mL syringe (Slip Tip with Intradermal Bevel Needle, 26G x 3/8; BD Syringe) and delivered daily by i.p ...
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bioRxiv - Immunology 2024Quote: ... cells were stained with AlexaFluor 647-rabbit anti-active caspase-3 antibody (BD, 1:20 in 1X BD Perm/Wash buffer) for 30 minutes at 4°C in the dark ...
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bioRxiv - Immunology 2024Quote: Fluorescence activated cell sorting (FACS) and flow cytometry analysis were performed using a 3 marker panel including FVS510 Fixable Viability Stain (BD Biosciences), CD45 FITC (Clone 2D1 ...
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bioRxiv - Immunology 2024Quote: Congenically marked WT or IL-27R-/- OT1 T cells were flow sorted 3 days after subunit vaccination using a FACSAria Fusion (BD Biosciences) and total RNA was isolated using Qiagen RNeasy columns ...
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bioRxiv - Immunology 2024Quote: ... in 75μl of 1xPBS for one hour were seeded on coated coverslips for 3 min at 37°C and fixed using Cytofix/Cytoperm-Fixation/Permeabilization Kit (BD biosciences). Coverslips were then gently washed two times with 1x PBS ...
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bioRxiv - Immunology 2024Quote: ... 50 μl of PBS-BSA containing 2.5 μl of CD11c APC antibody (clone S-HCL-3) or 5 μl of CD11c BV421 antibody (clone B-ly6 RUO) (BD Biosciences) were added to each tube ...
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bioRxiv - Neuroscience 2024Quote: ... sections were blocked with 3% animal serum in PBS-T for 1h and then incubated with anti-Ly6G (BD Bioscience, #551459) primary antibody overnight at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... cells were sorted to select double fluorescence positive clones (eGFP-Lysenin and mOrange-Galectin-3) using a BD FACSAria III Cell Sorter (BD Biosciences). A polyclonal subpopulation with high expression of eGFP-Lysenin was further selected for expansion.
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bioRxiv - Immunology 2024Quote: ... and then stained with a surface marker antibody cocktail (Supplemental Table 3) using BD Brilliant Staining Buffer (BD Biosciences, PN 563794) for 30 minutes at 4°C ...
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bioRxiv - Immunology 2024Quote: ... blocked for 30 minutes at RT (3% BSA, 10% SFB in PBS) and incubated with the following primary antibodies: anti-DUSP3 (1:500, BD Biosciences), anti-DUSP3 (1:50 ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then stained with cell phenotyping antibodies in a 1:1 volume ratio of 3% FBS and Brilliant Stain Buffer (BD Biosciences) according to standard procedures and analyzed on a FACS Aria III flow cytometer (BD Biosciences) ...
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bioRxiv - Bioengineering 2024Quote: The expression of the exhaustion markers PD-1 and TIM-3 was evaluated using anti-PD1 (cat. 560795, BD Biosciences, BD PharMingen) and anti-TIM3 (cat ...
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bioRxiv - Cancer Biology 2024Quote: Cells were analysed on the BD LSR Fortessa X-20 or BD FACSymphony or sorted on the BD FACSAria Fusion 5 or BD FACSAria Fusion 3 (BD Biosciences) at the Peter MacCallum Cancer Centre flow cytometry core facility ...
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bioRxiv - Cancer Biology 2024Quote: ... the animals were sacrificed by isoflurane overdose and blood was collected from the portal vein using 24G needles (BD Microlance 3) into microtubes with clot activator (Microvette 500 CAT-Gel ...
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bioRxiv - Cancer Biology 2024Quote: ... Final cell pellets were resuspended at a cell density of 2×106 cells/ml in 3% FBS in PBS and analyzed at the UTSW Flow Cytometry Core Facility (FACSCalibur, BD Biosciences). FlowJo software was used to analyze mean fluorescent intensity (RRID:SCR_008520).
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Planarian CREB-binding protein (CBP) gene family regulates stem cell maintenance and differentiationbioRxiv - Developmental Biology 2020Quote: ... which was incubated for 2 h in the dark at 37°C with 20 µM caspase-3 substrate Ac-DEVD-AMC (BD Biosciences Pharmingen) or 2 ml from a stock of 1 mg/ml for a final volume of 150 µl ...
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bioRxiv - Developmental Biology 2022Quote: Zebrafish wild-type and vsxKO retina sections from different developmental stages were analysed for the detection of apoptotic and mitotic cells using rabbit anti-active caspase-3 antibodies (BD Biosciences, 559565) and rabbit anti-phospho-histone H3 antibodies (Merck Millipore ...
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bioRxiv - Immunology 2021Quote: ... cells were stained for surface markers (when required) then fixed/permeabilized by using PE Active Caspase-3 Apoptosis Kit (BD Bioscience, 550914) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Between 2 - 4x105 events were collected per sample within 3 hours after staining on an LSRFortessa (BD Biosciences, 4 lasers, 18 parameters) and analyzed using FlowJo software (version 9.7.4 ...
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bioRxiv - Cancer Biology 2020Quote: ... Mammary fat pad injections were performed in the 4th pre-cleared mammary fat pad of 3 weeks old female mice in 50% matrigel (BD Biosciences, Germany). Breast or prostate cancer-origin of xenografts were verified by a pathologist.
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ACE2-lentiviral transduction enables mouse SARS-CoV-2 infection and mapping of receptor interactionsbioRxiv - Microbiology 2021Quote: ... Lentivirus was titrated by serial dilution of lentivirus and incubating with 5000 HEK293T cells in 96 well plates with 8 µg/ml polybrene for 3 days followed by GFP detection by flow cytometry (BD Biosciences LSRFortessa). Transduction units (TU ...
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bioRxiv - Immunology 2022Quote: ... 1x105 tumor cells and 1x105 γδ T cells were co-cultured for 3 h at 37°C in the presence of 1 µl α-CD107a-PE antibody (BD Biosciences). Medium or stimulation with α-CD3 and α-CD28 (both 1 µg/ml ...
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bioRxiv - Immunology 2022Quote: ... PBMC from the 3-time point from each patient were labelled using Single Cell Labelling with the BD Single-Cell Multiplexing Kit (BD Biosciences, #633781) and 52 different BD AbSeq Ab-Oligos reagents following the manufacturer’s protocol (BD Biosciences) ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were suspended in 500 μl PBS with 3 % FBS and then detected by flow cytometry (BD Biosciences, San Diego, CA, USA).
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bioRxiv - Neuroscience 2020Quote: COs were washed 3 times for 5 minutes in 5 mL of PBS in 5 mL round bottom tubes (BD Falcon, #1152367) then fixed overnight in 4% PFA at 4°C on a rocker ...
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bioRxiv - Immunology 2020Quote: ... and 3-Iodo-L-tyrosine-treated B cells were labeled by using the Annexin V Fitc Apoptosis detection kit (BD, Heidelberg, Germany) according to manufacturer`s instructions ...
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bioRxiv - Immunology 2022Quote: T cells were isolated as described above from the intestine of 3 individual samples and PLZF+ CD4+ TCR-β+ T cells were sorted on proxy surface markers using FACSAria2 SORP (BD Biosciences) as previously described (14) ...
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bioRxiv - Molecular Biology 2022Quote: ... The dermis was incubated in the enzyme mix from the whole skin dissociation kit (Milteney Biotec) for 3 hours according to the manufacturer’s instructions and further processed by Medicon tissue disruptor (BD Biosciences, Stockholm, Sweden). The dermal cell suspension was incubated with CD90 Microbeads ...
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bioRxiv - Cell Biology 2022Quote: ... maintained for 20 min on ice and then homogenized with syringe every 5 min (BD Microlance™ 3 Needle 0,5 × 25 mm). Nuclear lysate supernatants were collected at 1000 rpm for 10 min at 4°C 43.
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bioRxiv - Physiology 2022Quote: ... Venous peripheral blood (4-6 ml for adults, 1- 3 mL for children and infants) was collected on-site on EDTA (BD vacutainer©) and immediately stored at 4-8°C until use ...
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bioRxiv - Immunology 2022Quote: ... Intracellular staining was carried out with the following antibodies diluted in BD Perm/Wash buffer: Active-caspase-3-GranzymeB-PE (C92-605, BD, cat #561011), Active-caspase-3-BV650 (C92-605 ...
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bioRxiv - Cancer Biology 2022Quote: ... The cells were stained for 30 min on ice with 5 μl of biotin-labeled anti–cleaved caspase-3 monoclonal antibody (BD Biosciences, #550821). The cells were washed in Perm/Wash buffer (BD Biosciences ...
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bioRxiv - Neuroscience 2024Quote: ... N2a-ssGT cells were imaged for GFP fluorescence using CytationTM 3 Cell Imaging Multi-Mode Reader (BioTekTM) and flow cytometry analysis by Accuri® C6 flow cytometer (BD Biosciences).
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bioRxiv - Microbiology 2024Quote: ... and HIV-1 NL4-3-GFP-I virus by plating 1.5 × 106 cells per well of a 6-well flat-bottom plate (BD Bioscience, X, Y) and adding 250 mU RT (equivalent to 45 ng p24 ...
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bioRxiv - Microbiology 2024Quote: ... as well as HIV-1 NL4.3-GFP-I virus by plating 1.5 × 106 cells per well of a 6-well flat-bottom plate (BD Bioscience, X, Y) and adding 250 mU RT (equivalent to 45 ng p24 ...
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bioRxiv - Cancer Biology 2024Quote: ... Flow cytometry was performed on a 3 laser Cytek Aurora Cytometer (Cytek Biosciences, Fremont, CA) and the data was analyzed using FlowJo software (BD Biosciences, RRID:SCR_008520). All listed antibodies for flow cytometry are from Biolegend ...
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bioRxiv - Systems Biology 2023Quote: ... Each row of a 96-well plate is a single BD construct (For example, row A: BD construct #1, row B: BD construct #2, row C: BD construct #3… to row H: BD construct #8) and each column is a single AD construct (For example column 1 ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue was weighed then homogenized for 3 minutes in a bullet blender and serum was added to a serum separator tube (BD Microtainer: 365967) before being spun down and diluted 1:200 ...
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bioRxiv - Microbiology 2024Quote: ... Unspecific binding of antibodies was blocked by incubating the samples with 3% rat serum in BD staining buffer (BD Biosciences, Heidelberg, Germany) for 5 minutes at RT ...
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bioRxiv - Cancer Biology 2024Quote: ... and VC3ai cleavage by caspase-3/7 was assessed based on fluorescence emission at 530 nm using a BD LSR Fortessa™ Cell Analyzer (BD Biosciences).
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bioRxiv - Immunology 2024Quote: ... cells were suspended in 0.5 mL PBS+3% FBS and stained for flow cytometry analysis by adding anti-CD16/CD32 antibody (BD Pharmingen; clone 2.4G2) ,anti- CD117 (Biolegend clone 2B8) ...
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bioRxiv - Neuroscience 2024Quote: ... Tracheal intubation was performed after sevoflurane exposure (5%, 3 minutes) using a 20-gauge intravenous catheter (REF 382434; BD Angiocath Plus, Singapore). To ensure proper positioning of the catheter so as to avoid one- lung ventilation and gas leakage during ventilation ...
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bioRxiv - Bioengineering 2024Quote: The expression of the exhaustion markers PD-1 and TIM-3 was evaluated using anti-PD1 (cat. 560795, BD Biosciences, BD PharMingen) and anti-TIM3 (cat ...