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Citations for Becton, Dickinson and Company :
5401 - 5447 of 5447 citations for Mouse Serine threonine protein kinase PINK1 mitochondrial PINK1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... A third of each cell sample was stained for 30 mins at 4°C with 1:100-dilutions of APC-conjugated anti-C-Kit/CD117 (BD Biosciences; 550412) and PE-Cy7-conjugated anti-CXCR-4/CD184 (BD Biosciences ...
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bioRxiv - Cancer Biology 2024Quote: ... Myelin-depleted single-cell suspensions obtained from high grade glioma samples were sequentially labeled using the BD Human Sample Multiplexing Kit (Sample-tag) (BD Biosciences, #633781), the sorting antibody CD45-APC (#560973 ...
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bioRxiv - Microbiology 2024Quote: ... The cells were then resuspended in 500 μL of PBS and then stained following the Fixable Viability Stain 450 kit manufacturer instructions (BD Biosciences, 562247). Infected and uninfected macrophages were stained with Fixable Viability Stain 450 ...
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bioRxiv - Immunology 2024Quote: Intracellular staining for BIM was performed with secondary Donkey anti-rabbit APC secondary antibody using BD cytofix/cytoperm kit (BD biosciences 554722) per manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... Samples were washed twice in staining media and stained for intracellular cytokines or for Foxp3 according to manufacturer recommendations with BD Cytofix/Cytoperm Fixation/Permeabilization Kit (BD 554714, AB_2869008). Samples were further stained with conjugated intracellular antibodies (1:100 ...
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bioRxiv - Cancer Biology 2021Quote: ... The analysis was repeated three times according to the manufacturer’s instructions of Annexin V-FITC/PI kit (BD ISRI Sorp, Franklin Lakes, USA).
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bioRxiv - Cancer Biology 2021Quote: ... Samples were stained with 10 μg/ml propidium iodide (PI) staining solution from FITC Annexin V Apoptosis Detection Kit (BD Pharmingen; Cat#: 556547) and 0.2 mg/mL RNase A (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2020Quote: ... BrdU solution was added in culture medium and incubated for desired length of time to pulse the cells and further processed as per manufacturer’s instruction (BD Pharmigen BrdU Flow Kit). Stained cells were acquired on a flow cytometer.
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bioRxiv - Cancer Biology 2022Quote: ... The apoptotic rates were analyzed by the Annexin V-FITC and propidium iodide (PI) double detection kit (BD Biosciences, San Jose, CA, USA), and the rinsed cells were resuspended in binding buffer containing Annexin V-FITC and PI and then incubated at RT for 15 min in the dark ...
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bioRxiv - Cancer Biology 2019Quote: ... For apoptosis analysis was performed by a double staining Annexin V and 7-AAD Apoptosis Detection Kit (BD Pharmingen, San Jose, CA, USA) following the manufacture’s introductions ...
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bioRxiv - Cell Biology 2019Quote: ... the plasma levels of pro-inflammatory cytokines IL-6 and TNFα were quantified by bead-based flow cytometry assay (CBA Kit; BD Biosciences, Heidelberg, Germany) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2020Quote: Apoptosis was analyzed 24 hours after treatment (same concentrations as the CFU assay) using a FITC-AnnexinV/Propidium Iodide (PI) Apoptosis Detection kit per manufacter’s protocol (BD Biosciences, San Jose, CA) and as previously described using the anti-human CD34 antibody11 (Clone 581 ...
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bioRxiv - Immunology 2020Quote: ... Cell debris and dead cells were excluded from the analysis based on scatter signals and use of Fixable Blue or Green Live/Dead Cell Stain Kit (BD Bioscience, cat # 564406).
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bioRxiv - Cell Biology 2022Quote: ... Cells were resuspended and stained using cell surface antibody markers preconjugated to a fluorophore: KIT (CD117, BD Pharmingen 25-1171-82, RRID: AB_469644), CD45.2 (BD Biosciences 553772 ...
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bioRxiv - Cancer Biology 2022Quote: The apoptotic rate was determined using Hoechst 33342 staining following fluorescence-microscopic evaluation as well as by annexin V– FITC staining kit (BD Pharmingen, CA, USA) following the manufacturer’s instructions.HCT-116、HCT-116 Bax−/− cells (1×105 cells/well ...
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bioRxiv - Immunology 2024Quote: ... the cells were fixed and permeabilized using the BD Cytofix/Cytoperm™ kit as per the manufacturer’s instructions (BD Biosciences, San Jose, CA). Stained cells were washed twice and data were acquired on a BD LSR Fortessa X-20 (BD Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by washing with PBS and fixation/permeabilization using the BD Cytofix/Cytoperm kit according to the manufacturer’s protocol (BD Biosciences, San Jose, California). Cells were then pelleted and resuspended in the Click-iT OP-puro reaction cocktail with AlexaFluor 488 picolyl azide (Thermo Fisher ...
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bioRxiv - Cancer Biology 2023Quote: ... Intracellular staining was performed after fixation and permeabilisation of the cells using FOXP3 Fixation/Permeabilisation Solution kit (BD Biosciences, Frankin Lakes, NJ, USA) using manufacturer’s instruction ...
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bioRxiv - Pathology 2023Quote: ... The culture medium was sampled by adding 1-3 drops to microscope slides followed by Gram stain (BD BBLTM Gram Stain Kits; B12539) and imaging.
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bioRxiv - Cell Biology 2021Quote: ... for 10 min at 37°C and dead cells were labeled using the LIVE/DEAD Fixable Aqua (405 nm) Dead Cell Stain Kit (BD Biosciences, San Jose, CA). EdU detection ...
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bioRxiv - Cancer Biology 2022Quote: ... The co-cultures were incubated for 6 hours at 37°C and 5% CO2 prior to cell staining using the Cytofix/Cytoperm Fixation Kit (BD Biosciences, Cat# 554715/555028).
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bioRxiv - Neuroscience 2021Quote: ... Stimulated cells were stained with fluorochrome-conjugated anti-MCP-1 and anti-IL-6 Abs using the Cytofix/Cytoperm kit (BD Biosciences, San Jose, CA) to determine MCP-1 and IL-6 production ...
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bioRxiv - Physiology 2021Quote: ... islets were dispersed and dead cells were labeled using the LIVE/DEAD Fixable Aqua (405 nm) Dead Cell Stain Kit (BD Biosciences, San Jose, CA). EdU detection ...
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bioRxiv - Immunology 2021Quote: ... T2 and its derived cell lines were fixed and permeabilized using the Cytofix/Cytoperm kit (BD Biosciences, Becton, Dickinson and Company, Franklin Lakes, NJ). Washed cells were then resuspended in 1x PermWash buffer (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... followed by a cell viability stain (either Live/Dead Fixable Aqua Dead Cell Stain Kit, 405 nm excitation, Invitrogen ref. no. L34966; or Fixable Viability Stain 700; BD Horizon Cat. No. 564997). For tumor cell analysis ...
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bioRxiv - Cancer Biology 2022Quote: Cell viability was determined using a resazurin assay and cell death measured using the Annexin-V FITC apoptosis detection kit (BD Biosciences, San Jose, CA). Methods have been previously described (65 ...
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bioRxiv - Immunology 2021Quote: ... PM-WCPV-S and PM-CVHSA-S respectively) in the presence of Brefeldin A (0.65 µL/mL, GolgiPlugTM, BD Cytofix/Cytoperm Kit, Cat. 555028), co-stimulatory antibodies anti-CD28 (BD Biosciences Cat ...
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bioRxiv - Cell Biology 2022Quote: ... and their viability was tested using the annexin-PI apoptosis detection kit (MBL, USA) followed by flow cytometry analysis (BD Biosciences FACS Canto II).
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bioRxiv - Pathology 2021Quote: ... The cells were stained with fluorescein isothiocyanate (FITC)-conjugated annexin V and propidium iodide (PI) using the Annexin V-FITC & PI Apoptosis Detection Kit (, BD 货号 Biosciences, USA).
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bioRxiv - Biochemistry 2020Quote: ... and TNF in the supernatants was determined by using Cytometric Bead Array (CBA) - Human Inflammatory Cytokines Kit (551811, BD Biosciences, San Jose, CA, USA), that allows the determination of the indicated human cytokines simultaneously ...
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bioRxiv - Immunology 2021Quote: ... T cells were stained for surface antigens prior to fixation and subsequently processed for intracellular cytokine staining following BD Cytofix/Cytoperm™ kit (BD Biosciences cat 554714) instructions.
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bioRxiv - Physiology 2022Quote: ... Then final cDNA library was generated from double strand full length cDNA by random priming amplification with BD Rhapsody cDNA Kit (BD Biosciences, Cat. No. 633773) and BD Rhapsody Targeted mRNA & AbSeq Amplification Kit (BD Biosciences ...
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bioRxiv - Genetics 2023Quote: ... were isolated by 1X Red Blood Cell lysis Buffer (eBioscience) and stained with a combination of fluorochrome-conjugated antibodies: c-Kit (BD Biosciences, BioLegend clone 2B8), Sca-1 (BioLegend clone D7) ...
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bioRxiv - Cell Biology 2023Quote: ... density centrifugation or 1X RBC Lysis Buffer (eBioscience) and stained with a combination of fluorochrome-conjugated antibodies: c-Kit (BD Biosciences, BioLegend clone 2B8), Sca-1 (BioLegend clone D7) ...
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bioRxiv - Immunology 2023Quote: ... Following surface staining cells were fixed and permeabilized using a BD Cytofix/ Cytoperm Fixation/ Permeabilization Solution kit (Cat# 554714 BD Biosciences, Franklin Lakes NJ) followed by intracellular staining with anti-IFNγ ...
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bioRxiv - Genomics 2024Quote: ... the CD34+ cells were carefully pipetted out and their viability was measured using Annexin V apoptosis assay kit (BD Pharmingen, San Diego, CA, USA) as per the manufacturer’s protocol and counter stained with DAPI.
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bioRxiv - Neuroscience 2024Quote: ... PCR amplification of the fragments and their subsequent ligation was performed using the In-Fusion cloning kit and online tools (BD Clontech, Takara Bio, USA), or they were synthesized by GenScript (Piscataway ...
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bioRxiv - Biochemistry 2022Quote: ... cells were fixed with 1% (w/v) paraformaldehyde and 70% (v/v) ethanol on ice and stained with an APO-BrdU TUNEL assay kit (BD Biosciences, San Jose, CA, USA) according to the manufacturer’s protocol and with 1 μg/mL DAPI for 10 min ...
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bioRxiv - Immunology 2019Quote: ... mice were sacrificed and the cells from spleen and peripheral lymph nodes were stained for surface markers and then for BrdU according to the manufacturer’s protocol (BD Pharmingen, APC BrdU flow kit 552598).
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bioRxiv - Immunology 2021Quote: ... or PMA + ionomycin in the presence of brefeldin A for 4 h and stained using the Cytofix/Cytoperm Kit (BD Bioscience, Franklin Lakes, NJ, USA). Foxp3-positive cells and apoptotic cells were detected using the BD Foxp3 Staining Kit (BD Bioscience ...
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bioRxiv - Cancer Biology 2020Quote: ... The viability of the cells was measured post 48 hours of drug treatment using annexin-V-7AAD viability kit (BD Pharmingen, San Diego, CA, USA) as per the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2019Quote: ... and cells in the S phase using incubation with bromodeoxyuridine (BrdU) for 1 h and labeling with an anti-BrdU antibody (APC BrdU flow kit, BD Biosciences, San Jose, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Splenocytes taken from allo-transplanted recipients were stimulated with anti-CD3/anti-CD28 (2.5ug/ml) for 6 hours in the presence of Golgiplug (BD Cytofix/Cytoperm Plus kit cat#555028) (1:1000) ...
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bioRxiv - Microbiology 2022Quote: ... PM-WCPV-S and PM-CVHSA-S respectively) in the presence of Brefeldin A (0.65 µL/mL, GolgiPlug™, BD Cytofix/Cytoperm Kit, Cat. 555028), co-stimulatory antibodies anti-CD28 (BD Biosciences Cat ...
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bioRxiv - Cell Biology 2021Quote: ... permeabilization and EdU detection were performed using the Click-iT™ Plus EdU Alexa Fluor™ 488 Flow Cytometry Assay Kit (BD Biosciences, San Jose, CA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... bacterial Bacterial mortality was determined using the LIVE/DEAD® BacLightTM Bacterial Viability Kit (ThermoFischer Scientific, Ref. L7012) as described for flow cytometry utilization using an ACCURI C6 cytometer (BD Biosciences, Le pont de Claix,France) from the CYBIO Core Facility ...
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bioRxiv - Microbiology 2023Quote: DST for first line anti-tuberculosis drugs was performed on the BD BACTEC™ MGIT™ 960 automated mycobacterial detection system with the BD BBL™ MGIT™ AST SIRE kit (Becton, Dickinson and Company, NJ). All isolates were tested at the following critical concentrations as described by the manufacturer and the Clinical and Laboratory Standards Institute (CLSI ...