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Citations for Becton, Dickinson and Company :
5051 - 5100 of 8203 citations for 6 Methyl 2 3 4 9 tetrahydro 1H carbazole 1 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... For BrdU staining we used either a rat anti-BrdU (1:1000, Eurobio clone BU1/75) or mouse anti-BrdU (1:1000, BD 347580) antibodies 2 hours at RT (1:1000 ...
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bioRxiv - Immunology 2023Quote: ... The cells were washed with buffer (0.2% FBS in 1× PBS) and data (∼1 million events/sample) was acquired using BD LSRFortressa X-20 (BD Biosciences, USA). The acquired flow cytometry data were analysed using FCS Express (DeNovo software ...
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bioRxiv - Immunology 2023Quote: ... together with PE-anti-PD-L2 (clone TY25, 1:20) and its isotype control PE-Rat IgG2a,κ (clone R35-95, 1:20) were purchased from BD Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: ... were anesthetized with isofluorane and GFP-Luc labelled cells or MCF7-HypT cells resuspended in 1:1 PBS and Matrigel (BD Biosciences) were injected into the fourth mammary fat pad ...
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bioRxiv - Developmental Biology 2022Quote: ... 1:10000), anti-dendritic cell mAb: anti-CD11c (N418)-FITC (11-0114-81, eBioscience; 1:20) using LSR II (BD Biosciences). For OC differentiation ...
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bioRxiv - Cell Biology 2022Quote: ... 5 × 106 MCF-7 cells stably expressed shCtrl and shMYSM1 lentivirus were suspended in 100 μl of culture medium/Matrigel (1:1) (BD Biosciences) and implanted subcutaneously into the bilateral armpits of 4-week-old female BALB/c nude mice (Vital River Laboratory) ...
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bioRxiv - Cancer Biology 2023Quote: ... coverslips were incubated with primary antibodies (mouse anti-HA 1:200, Cell Signaling Technology, 2367; mouse anti-CtBP2, 1:250; BD Biosciences Cat# 612044 ...
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bioRxiv - Immunology 2023Quote: ... cells were stained for 30 min on ice with biotinylated recombinant HAs and PD-1-BB515 (EH12.1, BD Horizon, 1:100) diluted in P2 ...
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bioRxiv - Molecular Biology 2023Quote: ... 5.0 × 104 MDA-MB-231 or HCC1806-LM2C triple reporter-labeled cells were resuspended in a 1:1 mixture of PBS and Matrigel (BD Biosciences), and injected into the 4th mammary fat pad of female NOD-SCID-g female mice 6-8 weeks of age ...
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bioRxiv - Cancer Biology 2023Quote: ... and/or 0.25 million A431 cells in a 100 μL cell suspension of a 1:1 (v/v) mixture of medium with reconstituted basement membrane (BD Matrigel, BD Biosciences). Mice-bearing NCIN87/A431 bilateral tumors were developed by subcutaneous injection of NCIN87 and A431 cancer cells on the bilateral dorsal flank regions ...
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bioRxiv - Microbiology 2023Quote: ... Crypts in the tube were collected after removing the supernatant, and resuspended in 50% L-WRN conditioned medium (CM, Table S3) mixed 1:1 with Matrigel (BD Biosciences). The crypt suspension (55 μl per well ...
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bioRxiv - Immunology 2023Quote: ... and anti-MCL-1 A647 (1:1000, clone 19C4-15, WEHI antibody facility) and analysed on a Fortessa X20 (BD Biosciences) or Cytek Aurora spectral flow cytometer (Cytek Biosciences) ...
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bioRxiv - Microbiology 2024Quote: ... washed with PBS-T and kept overnight in 1% BSA solution in PBS-T containing a 1:500 dilution of anti-rabbit-Vi-polysaccharide antibody (BD BioSciences) overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... The cells were resuspended in T cell stimulation media (RPMI 1640 supplemented with 10% FBS, 1% Penicillin/Streptomycin, Protein Transport Inhibitor (Containing Brefeldin A) (diluted 1:1000) (BD, 555029), Monensin (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... cells were washed and resuspended in PBS/2% FBS for analysis on a five-laser flow cytometer (FACSymphony A3 Cell Analyser, BD Bioscience, San Jose, CA, USA). Data were analyzed with FlowJo software (TreeStar ...
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bioRxiv - Neuroscience 2020Quote: Neurons from 18-day-old rat embryos were dissected and dissociated using trypsin and plated at a density of 70,000 cells cm−2 in minimal essential medium (MEM) supplemented with 10% NU serum (BD Biosciences, Le Pont de Claix, France), 0.45% glucose ...
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bioRxiv - Immunology 2021Quote: ... and intracellular cytokines (PE anti-mouse IFN-γ, PE-Cy®7 anti-mouse TNF-α, APC anti-mouse IL-2, all from BD Biosciences, USA). The labeled cells were acquired with the BD FACSCanto™ II flow cytometer and analyzed with the program FlowJo v.10.6.2 (BD Biosciences).
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... we allowed organotypic cultures to differentiate at an air-liquid interface (ALI) in a defined differentiation media [base media with 2% v/v NuSerum (BD BioSciences, San Jose, CA, USA), and 50 nM retinoic acid (Sigma-Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... we allowed organotypic cultures to differentiate at an ALI in a defined differentiation media supplemented with IL-13 [base media with 2% v/v NuSerum (BD BioSciences, San Jose, CA, USA), 50 nM retinoic acid (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: The actinobacteria were routinely cultured from frozen spore preparations via inoculation into Difco International Streptomyces Project (ISP) medium 2 (ISP2) (Becton, Dickinson and Company, Spark, MD) and incubated at 28° C ...
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bioRxiv - Immunology 2020Quote: ... before being washed and re-suspended in PBS/2% FCS for analysis on a five-laser flow cytometer (LSR FortessaTM; BD Bioscience, San Jose, CA, USA). The data were analysed with FlowJo software (TreeStar ...
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bioRxiv - Immunology 2020Quote: Cells were stimulated for 12h with SARS-CoV-2 peptide pools (0.25 μg/ml) in the presence of αCD28/αCD49d co-stimulatory antibodies (BD FastImmune™, BD Biosciences, San Diego, CA, USA), 5 μg/ml brefeldin A (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... BD 560046. After washing twice, cells were fixed in PFA 2% for 20 min, washed, and acquired on a Fortessa BD LSRII Cell Analyzer (Becton Dickinson).
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bioRxiv - Molecular Biology 2023Quote: ... PMVECs were purified after two rounds of immune selection using magnetic beads coated with anti-CD31 and anti-ICAM-2 antibodies (BD Bioscience, Franklin Lakes, NJ, USA). mPMVECs were cultured and maintained in a medium containing 5 % FBS and EC growth supplement (ECGS ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were then washed 3x in flow cytometry stain buffer and fixed with 2% PFA for 20 minutes prior to acquisition on a LSRII Fortessa (BD Biosciences, San Jose, CA, USA). Samples were analyzed with FlowJo V10 software (Figure S4).
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bioRxiv - Immunology 2023Quote: ... 5.0 × 104 CD4+ T cells were serially diluted onto either α-IL-2 or α-IFNɣ sterile coated PVDF 96 well plates (BD Biosciences, NJ, USA). The CD4+ T cells were then activated for 24 or 72 h in complete DMEM ...
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bioRxiv - Cancer Biology 2023Quote: ... or E0771 hPD-L1 (2 × 105 cells in 25 μL of medium mixed with 25 μL of Matrigel Basement Membrane Matrix [BD Biosciences, San Jose, CA, USA]) were injected into the mammary fat pad or flank ...
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bioRxiv - Genetics 2022Quote: ... The proportion of HbF-and HbS-positive RBCs were measured with an antibody recognizing HbF (Thermo Fisher for HUDEP-2 cells or BD Biosciences for primary cells) or HbS (BioMedomics) ...
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bioRxiv - Cancer Biology 2021Quote: ... and resuspended in 50 µL of FACS buffer (PBS plus 1% BSA) and blocked with µL 1 human Fc block (BD Biosciences, 564219) for 20 minutes at 4°C ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated for 30 min on ice with biotinylated and Alexa Fluor 647-conjugated recombinant soluble S and PD-1-BB515 (EH12.1, BD Horizon, 1:100) in 2% FBS and 2 mM EDTA in PBS (P2) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were fixed and permeabilized prior to staining with primary antibody (Vimentin CST # 1:100 and E-cadherin BD number 1:100) overnight followed by secondary (anti-rabbit ThermoFisher #31466 1:10000 ...
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bioRxiv - Neuroscience 2019Quote: ... Blocking was followed by 1-hour incubation with Anti-Pan Nav (Alomone labs, # ASC003) (1:50) and purified Mouse Anti-MAP II (BD biosciences, #556320) (1:400 ...
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bioRxiv - Neuroscience 2020Quote: ... one-in-ten sections were incubated with different anti-BrdU antibodies (BrdU & CldU, rat primary antibodies at 1/200 Accurate Chemical; IdU, mouse primary antibodies at 1/200, BD Biosciences). Sections were also incubated with Zif268 (rabbit,1:500 ...
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bioRxiv - Immunology 2021Quote: 12-week old mice were sensitized by intradermal injection of 100ug rat collagen type II (COL2) in 100ul of a 1:1 emulsion with Complete Freund’s adjuvant (BD, Difco, MI, USA) and 10mM acetic acid at the base of the tail ...
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bioRxiv - Immunology 2021Quote: ... 100 µL of each collected serum sample diluted 1:100 with 1% (w/v) Difco Skim Milk (cat. n° 232100, BD Biosciences, USA) was added to each plate for 1 h at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... rat anti C4b (thermo fisher scientific MA1-40047,1:25) rabbit anti Olig2 (millipore AB9610, 1:250) mouse anti-Gstp (BD 610719, 1:250), anti mouse 555 (thermo fisher scientific A-21422 ...
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bioRxiv - Cell Biology 2022Quote: ... washed with PBS and incubated in DMEM media containing 1% FBS and 1% BSA with Alexa Fluor® 647 Mouse Anti-Human LAMP1 Clone H4A3 (BD Biosciences) for 1h at 4°C with intermittent mixing ...
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bioRxiv - Neuroscience 2021Quote: ... The cell pellet was then stained with antibodies to microglial cell surface markers (CD11b-PE Cy7, 1:200 Biolegend, # 101216; CD45-APC Cy7, 1:200, BD Biosciences # 103116;) for isolation using the FACSAria™ III cell sorter (Fig 1C) ...
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bioRxiv - Cancer Biology 2021Quote: ... The fibres were stained with primary antibodies (Rat anti-BrdU Abcam ab6326 1:250, Mouse anti-BrdU BD Biosciences 347580 1:100) overnight and with secondary antibodies (Alexafluor 555 goat anti-rat 1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti-Tuj1 (gift from Tony Spano, University of Virginia, 1:250) and rat anti-Icam-II (BD Pharmingen, 553325, 1:500). Secondary antibodies ...
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bioRxiv - Bioengineering 2022Quote: ... the membrane was incubated overnight at 4°C or with primary antibody solution (1:1000 dilution for anti-Alix [E4T7U, Cell Signaling Technology], anti-QSOX1 [12713-1-AP, ProteinTech], anti-HIF-1α [610958, BD Biosciences] ...
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bioRxiv - Biochemistry 2022Quote: ... The cells were washed 1× with 1ml cold PBS and resuspended in 500μl PBS containing PE Streptavidin (BD 554061: 1:200 dilution) and Zombie Violet live dead stain (BioLegend 423113 ...
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bioRxiv - Immunology 2022Quote: ... Then the cells underwent membrane surface staining for 30 minutes at 4°C protected from light with Fixable Viability Dye eFluor506 (1:1000, eBiosience, Cat# 65-0866-14) and the following antibodies: CD3 BUV805 (1:50, BD, Cat# 612895), CD8 BUV496 (1:50 ...
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bioRxiv - Neuroscience 2024Quote: ... The following primary antibodies were used in this study: anti-total α-syn mouse monoclonal Syn-1 (1:10,000 dilution; BD Biosciences #610786), anti-PSyn rabbit monoclonal EP1536Y (1:10,000 dilution ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were cultured in RPMI plus 10% FBS in 2D on tissue culture plates or in 3D in non-adherent suspension plates in a 1:1 mix of Matrigel (growth factor-reduced, BD Biosciences - 354230) and RPMI plus 10% FBS ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were blocked in 5% milk in 0.2% Tween-20 in TBS for 1 h at room temperature and probed with primary antibodies against SORLA (1:4000; 611680, BD Transduction Labs) and GAPDH (1:10,000 ...
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bioRxiv - Immunology 2023Quote: ... DO11.10 TCR transgenic mice subcutaneously received 50 μL injections of a 1:1 emulsion of adjuvant complete Freund H37 Ra (BD Difco, 231131) and 2 mg/mL grade V ovalbumin (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... FITC-coupled anti-ST2 (1:100, clone DJ8, MD Bioproducts, #101001F) and PE-coupled anti-CD13 (1:100, clone R3-242, BD Pharmingen, #558745) antibodies ...
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bioRxiv - Immunology 2023Quote: ... or FITC-coupled anti-ST2 (1:100, clone DJ8, MD Bioproducts, #101001F) and PE-coupled anti-CD13 (1:100, clone R3-242, BD Pharmingen, #558745), respectively ...
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bioRxiv - Neuroscience 2023Quote: ... Eluent was washed twice, incubated with DR (Bio-Legend, 424101, 1:1000) and CD16/CD32 Fc block (BD Biosciences, 553141, 1:200) at room temperature for 15 minutes ...