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Citations for Becton, Dickinson and Company :
451 - 500 of 741 citations for Streptavidin magnetic beads since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: Bone marrow cells from mice were labeled with a cocktail of biotin-conjugated lineage marker antibodies in combination with Streptavidin Particles Plus (BD, 557812) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... After subsequent washing step cells were labelled with primary and secondary antibodies or streptavidin-fluorochrome conjugates and were used for flow cytometry analysis (LSR-II, BD Biosciences) or sorting (FACSAria ...
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bioRxiv - Genomics 2022Quote: ... This was followed by the secondary labeling step with 1:200 dilution of Streptavidin PE-Cy7 antibody (BD Biosciences, Cat#: 557598). Cells were isolated and sorted using an Influx Cell Sorter Instrument (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... Plates were then washed and incubated with detection biotinylated anti-IFNγ antibody for 2h and followed by incubation with streptavidin-horse radish peroxidase (BD Biosciences) for 1h ...
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bioRxiv - Immunology 2020Quote: For data acquisition on the BD FACSymphony the following amounts of 14 different fluorescent streptavidin conjugates were added to 10 μl of pMHC monomer (100 μg/ml): 1 μl of SA-BB790 (BD, custom), 1 μl of SA-BB630 (BD ...
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bioRxiv - Immunology 2020Quote: ... Biotinylated control peptides were exchanged with HLA-DR at 1:2 molar ratio in the presence of 5 times excess of a test peptide and detected using horseradish peroxidase (HRP)-conjugated streptavidin (BD Biosciences). PBMCs obtained after vaccination from each donor were stimulated with 47 DR-binding peptides at 0.4ug/ml per peptide for 3 weeks and stained with tetramers ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated antibody bound to RBD in the presence of competitor was stained with Streptavidin-PE at a 1:1000 dilution (BD Biosciences) and analysed on a 200 Bioplex instruments ...
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bioRxiv - Immunology 2022Quote: ... Spike and RBD tetramers were prepared fresh before use and formed by combining biotinylated proteins with PE-conjugated Streptavidin (BD Biosciences) at a molar ratio of 4:1.
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bioRxiv - Immunology 2023Quote: ... Functionalised agarose beads were washed twice and then incubated with 20 nM of the GFP-streptavidin tetramers and anti-mouse IgG2a/b BV421 antibody (1:10 dilution, clone R2-40, BD Bioscience) for 30 min on ice ...
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bioRxiv - Cell Biology 2022Quote: ... for epitope retrieval and incubation with primary anti-Collagen Type II (DSHB II-II6B3) followed by a biotinylated secondary and Streptavidin-HRP (BD Biosciences). II-II6B3 was deposited to the DSHB by Linsenmayer ...
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bioRxiv - Immunology 2022Quote: ... ProT2 MHC Class II monomers for HLA-DRB1*15:01 (S751-767 NLLLQYGSFCTQLNRAL) were purchased from ProImmune and conjugated to Streptavidin-PE (BD Biosciences) at a molar ratio of 8:1 monomer:S-PE ...
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bioRxiv - Cell Biology 2024Quote: ... was added for 1 h at 37 °C followed by Streptavidin-conjugated Horse Raddish Peroxydase (HRP; BD Pharmingen; dilution 1:3000) for 1 h at 37 °C ...
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bioRxiv - Microbiology 2024Quote: ... plates were washed 4 times with PBST and filled each well with 100 µl 1000 times diluted HRP-conjugated streptavidin (BD, 554066) in 0.5% skim milk PBST buffer ...
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bioRxiv - Immunology 2020Quote: ... Mouse neutrophils were generally purified from the BM by positive magnetic selection with anti–Ly-6G PE-conjugated Ab (1A8; BD Biosciences) and anti-PE microbeads (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... Cells were enriched for marker gene expression using magnetic activated cell sorting (MACS; Miltenyi) or fluorescent activated cell sorting (FACS) on an Aria III cell sorter (BD Biosciences). Stimulator cells were loaded with peptides through pre-incubation for 30 minutes at 37°C with 0.01-1 µM peptide (Supplementary file 3 for peptide details) ...
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bioRxiv - Cancer Biology 2021Quote: ... Beads detecting cytokines from mouse were analyzed on a FACS Verse (BD Biosciences ...
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bioRxiv - Microbiology 2020Quote: ... beads were rolled on R2 agar plates (BD Difco, Sparks Maryland, USA) and incubated at 25 °C ...
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bioRxiv - Genomics 2020Quote: ... cell numbers were measured by flow cytometry using Calibrite beads (BD Pharmingen) as standards as previously described41 ...
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bioRxiv - Microbiology 2021Quote: The human inflammatory cytometric bead array (CBA) kit was purchased from BD Biosciences (San Jose ...
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bioRxiv - Immunology 2020Quote: Cytometric Bead Array for Th1/Th2/Th17 cytokines was obtained from BD biosciences and performed on 10µl supernatant after 4day co-culture following manufacturers’ instructions ...
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bioRxiv - Microbiology 2020Quote: ... Beads were analyzed using flow cytometry on a FACSAriaIII instrument (BD Biosciences).
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bioRxiv - Immunology 2022Quote: ... Cell counts were measured by flow cytometry using counting beads (BD Biosciences) and data were collected on a modified LSRII or a modified FACSymphony ...
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bioRxiv - Immunology 2022Quote: ... Single-stained controls using mouse Ig/ κ compensation beads (BD Biosciences, 552843) were used to calculate compensation ...
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bioRxiv - Biophysics 2022Quote: ... Additives were spacer beads (non fluorescent FACS calibration, ∅ 6 μm, BD Biosciences) and freely diffusing Atto-NHS 647N dye (ATTO-TEC ...
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bioRxiv - Molecular Biology 2023Quote: ... and the droplet stream was calibrated with Accudrop Beads (BD Biosciences, 345249). Sort layout was set to 4-way purity and four subfractions were collected into 5 mL FACS tubes as follows:
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bioRxiv - Cancer Biology 2023Quote: ... Receptor density measurements were performed using Quantibrite Beads (BD Biosciences, cat #340495), which contain four levels of phycoerythrin (PE ...
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bioRxiv - Genomics 2024Quote: ... the BD Quantibrite™ Beads PE Fluorescence Quantitation Kit (BD Biosciences; #340495) was used and CD19 expression in cells was assessed using a PE-conjugated antibody (PE anti-human CD19 ...
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IL-27R signaling serves as immunological checkpoint for NK cells to promote hepatocellular carcinomabioRxiv - Immunology 2020Quote: ... After washing with 1% BSA-PBS slides were incubated with secondary goat anti-rat and goat anti-rabbit biotinylated antibodies for 30 min at RT followed by 30 min of incubation with streptavidin–HRP (1:500; BD Pharmingen, 554066). For developing DAB substrate (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... The cells were washed 1× with 1ml cold PBS and resuspended in 500μl PBS containing PE Streptavidin (BD 554061: 1:200 dilution) and Zombie Violet live dead stain (BioLegend 423113 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The cells were washed twice with FC buffer by centrifugation (1000 rpm/5minutes) and incubated with streptavidin−PE conjugate (BD bioscience, 349023) for 30 minutes at 4ºC ...
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bioRxiv - Immunology 2024Quote: ... Content of chemokines / cytokines was quantified after washing and staining with biotinylated detection antibodies and phycoerythrin bound streptavidin by using LSR II (BD Bioscience, Germany). Calculation of each chemokine / cytokine quantity was determined by using standard curves according manufacturer’s instructions.
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bioRxiv - Microbiology 2024Quote: ... Plates were washed five times before adding rabbit polyclonal anti-biotin (ab68520 1:1000 dilution) and streptavidin-HRP (cat#554066, BD 1:1000) followed by 1-hour incubation at room temperature ...
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bioRxiv - Immunology 2024Quote: ... Biotinylated monomers of MR1-5-OP-RU or CD1d-α-GalCer were tetramerised to streptavidin conjugated to PE (SAV-PE; BD Pharmingen), BV421 (SAV-BV421 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... a yeast IgG library was subjected to multiple rounds of selection by magnetic activated cell sorting (MACS) and florescence activated cell sorting (FACS, BD ARIA III) in PBS buffer containing 1mM MnCl2 ...
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bioRxiv - Cell Biology 2022Quote: ... bone marrow cells were isolated and labelled using biotin-conjugated lineage antibody cocktail in combination with PerCP- conjugated streptavidin (BD, 554064; 1:200), and antibodies against SCA-1 ...
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bioRxiv - Immunology 2023Quote: ... S1pr2-CreERT2 were purified by depleting CD90+ T cells and CD138+ cells using biotinylated anti-Thy1.2 Ab and biotinylated anti-CD138 Ab followed by streptavidin-conjugated microbeads (iMagTM; BD Biosciences, San Jose CA). B cells from CD45.1 ...
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bioRxiv - Microbiology 2020Quote: ... SpA and C1q to the beads were detected using flow cytometry (BD FACSVerse) and data were analyzed based on single bead population using FlowJo software.
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bioRxiv - Microbiology 2020Quote: ... and fluorescence detector sensitivity using the BDTM CS&T beads (#656504, BD Biosciences). Drop delay before every FACS sort was determined using the BD FACSTM Accudrop beads (#345249 ...
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bioRxiv - Cancer Biology 2020Quote: ... Compensations were attained using Anti-Rat and anti-hamster compensation beads (BD Biosciences). For fixable live/dead staining ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples and beads were run on the Accuri C6 Plus cytometer (BD Biosciences), and receptor levels calculated based on the dye:protein ratio of the individual antibodies and values determined using the QuickCal spreadsheet (Bangs Laboratories).
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bioRxiv - Immunology 2022Quote: ... Cytokines were quantified using a cytometric bead assay (CBA mouse inflammation kit, BD) according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: BD cytometric bead array Mouse Th1/Th2 cytokine kit (Cat# 551287, BD Biosciences) was used to determine the IFN-γ levels ...
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bioRxiv - Immunology 2022Quote: ... using the Mouse Inflammation Cytometric Bead Array kit (reference: BD Biosciences, Rungis, France) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Stained cells and beads were analyzed on a BD FACSCanto II (BD Biosciences). The Geo Mean or Median channel values for each population were recorded into the provided QuickCal spreadsheet yielding a regression coefficient of 0.998.
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bioRxiv - Neuroscience 2020Quote: ... Analyte concentrations were measured by cytometric bead array (CBA; BD Biosciences, CA, USA) as described previously (85) ...
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bioRxiv - Microbiology 2020Quote: ... 4000 bead events were collected on a FACS Canto II cytometer (BD Biosciences) using BD FACS Diva software ...
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bioRxiv - Immunology 2022Quote: ... Bead uptake was measured by flow cytometry on a BD LSRII (BD Biosciences) and analyzed by FlowJo 10.3 ...
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bioRxiv - Immunology 2020Quote: ... Compensation for Zombie NIR was performed using BD Amine Reactive Beads (BD Biosciences) stained as per manufacturer protocols ...
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bioRxiv - Immunology 2021Quote: ... C1q or C3b to the beads was determined by flow cytometry (BD FACSVerse). Data were analyzed based on single bead population using FlowJo software and presented as Fluorescence Intensity (FI ...
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bioRxiv - Immunology 2021Quote: ... Stained cells or beads were analyzed using a flow cytometer (LSR II; BD) and data were analyzed using FLOWJO ...