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Citations for Becton, Dickinson and Company :
451 - 500 of 572 citations for 6 Fluoro 8 methoxyquinoline since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: CD44+CD62L+ and CD44-CD62L+ DN1 T cells were sorted from LNs of DN1-hCD1Tg BM chimera mice vaccinated with MA-BCN at 6 weeks post vaccination by BD FACS Aria with purity >98% ...
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bioRxiv - Immunology 2023Quote: ... approximately 4-6 × 106 isolated murine CD4+ T cells/well were stimulated in a 6-well plate pre-coated with 1μg/ml αCD3 (clone 145-2C11, BD Pharmingen) and 1μg/ml and αCD28 (clone 37.51 ...
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bioRxiv - Immunology 2023Quote: ... and 1 μg/ml 4′,6-diamidino-2-phenylindole (DAPI) and analyzed on a BD FACS Aria III (BD Biosciences).
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bioRxiv - Microbiology 2023Quote: ... were stimulated with peptide pools of ESAT-6 and CFP-10 (10μg/ml of each peptide pool) in the presence of Brefeldin A (GolgiPlug, BD Biosciences) at 37°C/5% CO2 for 3.5-4 hours prior to staining ...
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bioRxiv - Immunology 2024Quote: ... CD16 BV421 (clone 3G8, catalog #: 562878), HLA-DR BV786 (clone G46-6, catalog #: 564041), and CD15 BV510 (clone W6D3, catalog #: 563141) from BD Biosciences.
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bioRxiv - Immunology 2024Quote: ... T cells or NK cells were mixed with tumor cells for 6 h in the presence of GolgiPlug (BD, BDB555029). As a positive control ...
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bioRxiv - Microbiology 2024Quote: ... were selected for each sample (for a total of up to 6 colonies) and sub-streaked onto non-selective brain heart infusion agar plates (BHIA, BD), followed by incubation at 37°C for 48 h ...
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bioRxiv - Cancer Biology 2024Quote: ... with 4′,6-diamidino-2-phenylindole (DAPI) as a viability dye and the following antibodies: anti-CD31 PE (BD Biosciences), anti-CD45 APC (Fisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: NF1-proficient and NF1-deficient engineered clones were injected into the flanks of 6- to 7-week-old athymic nude mice (1.0×106 cells) in 50% Matrigel (BD Biosciences). Mice were monitored for tumor formation for 6 months post injection or until a tumor volume of 2000mm3 was reached.
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bioRxiv - Cancer Biology 2024Quote: ... bone marrow aspirates were performed every 6 weeks post-transplant and human chimerism was tracked by flow cytometry analysis (using a BD LSR Fortessa analyzer or BD FACSymphony ...
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bioRxiv - Cancer Biology 2022Quote: Trans-well assay was done in 24-well Boyden chambers with 8-μm pore size polycarbonate membranes coated with Matrigel (BD Biosciences, San Diego, USA). ST1 cells (5 × 104 ...
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bioRxiv - Neuroscience 2023Quote: Blood extractions were performed in the morning (8:00-10:00 a.m.) after 8-12 h of fasting and extracted into 10mL BD vacutainer® tubes with K2-EDTA anticoagulant (BD, Franklin Lakes, NJ, USA). Blood samples were immediately processed to obtain plasma and PBMCs based on our previous studies (Orio et al. ...
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bioRxiv - Biochemistry 2023Quote: Invasion assays were done in 8 μm pore size of the Chemotaxicell chamber coated with 100 μg/ml Growth factor reduced Matrigel (BD Biosciences, San Jose CA) for 3 h at 37°C and blocked with 0.1% bovine serum albumin in PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... Fluorescence values were converted to molecules of equivalent fluorescein (MEFL) by calibrating to SpheroTM 8-peak rainbow calibration particles (BD Biosciences, #559123, Lot #8137522). A calibration curve that relates arbitrary fluorescence intensities to MEFL was created by calculating the linear regression between measured relative fluorescence units of each bead distribution and the manufactured supplied MEFL of each distribution ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were co-stained with 4′,6-diamidino-2-phenylindole (DAPI) to measure DNA content and analyzed with a BD LSRFortessa flow cytometer (BD Biosciences) and FlowJo software (TreeStar).
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bioRxiv - Cell Biology 2020Quote: ... Percentage of platelets in BALF (n=3-6) was quantified on a BD FACSVerse flow cytometer (BD Biosciences, San Jose, USA) and analysed with FlowJo v10 software (FlowJo LLC ...
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bioRxiv - Immunology 2019Quote: ... The cecum was exteriorized and ligated with a 6.0 silk suture (6-0 PROLENE, 8680G; Ethicon) placed below the ileo-cecal valve and perforated with a 21-gauge needle (BD Biosciences) to induce mid-grade lethal sepsis78–80 ...
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bioRxiv - Immunology 2019Quote: ... 25 μL of cell suspension from each well was washed with 100 μL PBS-EDTA and stained with 5 μL of HLA-DR-FITC (clone G46-6, BD BioSciences) or isotype control (clone G155-178 ...
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bioRxiv - Cell Biology 2019Quote: ... cells were plated at 1/6 confluence in either six 175 cm2 flasks or in two three-layer 525 cm2 tissue culture flasks (BD Biosciences) and after 24 hours synchronized at different stages of the cell cycle as described in the previous section ...
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bioRxiv - Microbiology 2020Quote: ... lymphocytes were cultured in 96-well dishes at 37°C for 5-6 h in the presence of 2 μM peptide pool and brefeldin A (BD Biosciences). Cells were then labeled for cell-surface markers ...
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bioRxiv - Cancer Biology 2019Quote: ... the cells were re-stimulated for another 6 h with the anti-CD3 antibody in the presence of both GolgiStop and GolgiPlug (BD Biosciences) and stained with antibodies against IFN-γ and TNF-α ...
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bioRxiv - Immunology 2022Quote: ... Cells were pelleted by centrifugation at 600 x g for 6 minutes and fixed in 100 μL per 1×106 cells of PhosFlow Lyse/Fix buffer (BD Bioscience) at 37 °C for 10 minutes ...
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bioRxiv - Immunology 2022Quote: ... and the remaining cells were incubated for 5-6 hrs at 37°C in the presence of 1 uM of the indicated peptide and brefeldin A (GolgiPlug; BD Pharmingen). Surface staining was done overnight at 4 degrees ...
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bioRxiv - Immunology 2020Quote: ... Cells were incubated for 5-6 days and CFSE dilution of T cells was determined on a BD Canto II (BD Bioscience) and analyzed by FlowJo (v10.1 ...
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bioRxiv - Immunology 2020Quote: ... fibroblast-collagen cultures were prepared in Falcon cell culture inserts and placed into Falcon 6 Well Deep Well TC-Treated Polystyrene Plates (BD Biosciences) containing 2×105 fibroblasts in FBS ...
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bioRxiv - Immunology 2021Quote: ... spleen cells of immunized mice were incubated with 50 μM of each peptide for 6 hours at 37°C in the presence of monensin (GolgiStop, BD Biosciences) and 0.8 μg of FITC-conjugated anti-mouse CD107a mAb (BioLegend) ...
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bioRxiv - Immunology 2022Quote: ... Tracheas were stretched out by pinning to SylGard-coated well of 6 well plate and fixed for 1 hr on ice in Cytofix/Cytoperm buffer (BD Biosciences). Immunofluorescent staining was performed in PBS with 0.25% Triton X-100 at 4°C as follows ...
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bioRxiv - Immunology 2022Quote: ... and 10% heat-inactivated FBS) and incubated at 37°C for 6 hours with protein transport inhibitors GolgiStop and GolgiPlug (BD Biosciences) and S glycoprotein peptide pools 1 μg/mL (JPT product PM WCPV-S-1 ...
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bioRxiv - Neuroscience 2019Quote: ... IL-6 and MIP-2 were measured using paired antibodies and recombinant cytokines according to the manufacturer’s recommendations (BD PharMingen Systems) (11).
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bioRxiv - Microbiology 2020Quote: ... from the four subgroups were co-cultured in the Biolog AN Microplate in a pairwise fashion (n =6, AB, AC, AD, BC, BD, CD), by combining 50 μL of each isolate suspended in inoculation fluid in each well ...
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bioRxiv - Microbiology 2021Quote: ... lymphocytes were cultured in 96-well dishes at 37°C for 5-6 h in the presence of 2 μM peptide pool and brefeldin A (BD Biosciences). Cells were then labeled for cell-surface markers ...
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bioRxiv - Microbiology 2021Quote: ... according to the manufacturer’s protocol and intracellular staining was performed using anti-BCL-6-AF647 (clone K112-91, 1:100, BD, cat#561525).
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bioRxiv - Immunology 2019Quote: Emigrated cells were washed in PBS with 1% BSA and 0.02% NaN3 and incubated for 30 minutes on 4°C with subset markers HLA-DR BV510 (clone G46-6, BD biosciences), CD1a APC (clone HI149 ...
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bioRxiv - Bioengineering 2020Quote: ... HEK293 cells transfected with ABE plasmids were collected from 6-well plate and analyzed on Becton Dickinson LSR II (BD Biosciences) to determine GFP-positive cells ...
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bioRxiv - Cell Biology 2020Quote: ... the cell surface of freshly-prepared epidermal cells was labeled with the following antihuman protein antibodies (clone, manufacturer): FITC–conjugated CD1a (HI149) and PE–conjugated HLA-DR (G46-6, both BD Pharmingen), PE–CF594–conjugated CD3 (UCHT1 ...
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bioRxiv - Immunology 2021Quote: ... and circulating plasmablasts from 6 donor mice per group (ApoE-/- versus ApoE-/-Gpr55-/- mice) were sorted using a BD FACSAria III Cell Sorter (BD Biosciences) as described above and bulk RNA-sequencing was performed using the prime-seq protocol.26 A step-by-step protocol can be found on protocols.io (dx.doi.org/10.17504/protocols.io.s9veh66) ...
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bioRxiv - Pathology 2021Quote: ... 0.2 micron sterile filtered) using an insulin syringe (31-gauge, 6 mm length, 3/10 cc, BD Veo Ultra-Fine needle). Glucose and lactate levels were measured using a minimally invasive tail-prick method at designated timepoints post-injection.
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bioRxiv - Neuroscience 2021Quote: ... The suspended cell lysates were pipetted 6 times gently and passed through a syringe 20 times (1 ml TB Syringe, BD, USA), and avoid any bubble in the procedures ...
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bioRxiv - Microbiology 2021Quote: ... The lung infiltrated cells were cultured with purified RBD protein at 10 μg/ml for 12 h at 37 °C followed by a 6 h treatment with GolgiPlug (BD 555028). After blocking with FcγIII and FcγII receptors antibody (BD Pharmingen ...
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bioRxiv - Immunology 2020Quote: ... CD95, CD278) or intracellular proteins (BCL-2, BCL-6, CD152) and cells were acquired on an LSR II analyser (BD Pharmingen), followed by flow cytometric analysis using the FlowJo Software (FlowJo LLC ...
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bioRxiv - Immunology 2021Quote: Cells were co-cultured in R10 medium alone or in presence of K562 cells for 6 hours in the presence of anti-human CD107a (H4A3, FITC, BD Biosciences). Golgi plug and Golgi stop (BD Biosciences ...
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bioRxiv - Cell Biology 2022Quote: ... the endothelial cell monolayers in 6-well plates were lysed after removing center region using cell scraper (BD Falcon #35-3085) and washing with 1X HBSS (Corning #21-022-CV).
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bioRxiv - Neuroscience 2023Quote: ... single viable cells were gated and CD45highCD8+ cells were collected using a FACSAria III and corresponding software (FACSDiva, v.6; BD Biosciences). Calculation of the number of CD45highCD8+ T cells per brain was performed by extrapolating their frequency to the counted total number of isolated cells ...
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bioRxiv - Immunology 2022Quote: ... Supernatants were recovered and the amounts of IL-6 were analyzed by cytokine-specific ELISA kits (BD Biosciences, Mountain View, CA).
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bioRxiv - Neuroscience 2023Quote: ... single viable cells were gated and CD45-O1+ and CD45lowSiglec- H+ cells were collected using a FACSAria III and corresponding software (FACSDiva, v.6; BD Biosciences). Calculation of the number of CD45lowSiglecH+ microglia per brain was performed by extrapolating their frequency to the counted total number of isolated cells ...
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bioRxiv - Microbiology 2023Quote: Stm strain SL1344 with plasmid pMW211[C.10E/DsRed] [20] and strain 12023 with plasmid pluxCDABE [6] were recovered from frozen glycerol stock and cultured in Difco Luria-Bertani (LB) Broth (BD Biosciences) containing 100 µg/ml ampicillin (Merck ...
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bioRxiv - Biophysics 2022Quote: ... The FITC ERF values were assigned to both 2 μm and 6 μm beads using a specific lot of FITC-FC Bead (BD Biosciences) as a calibrator with known ERF value ...
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bioRxiv - Bioengineering 2023Quote: ... Circulating levels of IL-6 and TNF were determined using a cytokine bead array (BDTM Cytometric Bead Array Mouse IL-6/TNF Flex Set, BD Biosciences). Samples were analyzed in technical triplicates.
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bioRxiv - Bioengineering 2023Quote: ... Cells were then selected in puromycin (1μg/ml) 2 days after transduction and sorted for their EGFP fluorescence after 6 additional days using FACS ARIA III (BD Biosciences). Cells were grown for recovery and collected for genomic DNA extraction with DNeasy Blood and Tissue kit (Qiagen).
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bioRxiv - Cancer Biology 2023Quote: ... a single cell suspension was generated using trypsin and the cells were sorted based on GFP intensity into 6 equally logged bins using BD FACS Aria III (BD Biosciences). In a parallel control condition ...