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Citations for Becton, Dickinson and Company :
451 - 500 of 8593 citations for 3 Hydrazino 5 methyl 4H 1 2 4 triazol 4 ylamine hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... PE-IL-4 (clone 11B11, Cat#554435; BD Pharmingen, San Jose, CA, USA), FITC-IL17A (clone BL168 ...
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bioRxiv - Immunology 2024Quote: ... and Monensin protein transport inhibitors (4 pL/6E6 cells; GolgiStop, BD Biosciences, 554724) were added to the media ...
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bioRxiv - Neuroscience 2020Quote: COs were washed 2 times for 5 minutes at room temperature in 5 mL of PBS in 5 mL round bottom tubes (BD Falcon, #1152367) then fixed overnight in 4% PFA at 4°C on a rocker ...
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bioRxiv - Synthetic Biology 2023Quote: ... A third of each cell sample was stained for 30 mins at 4°C with 1:100-dilutions of APC-conjugated anti-C-Kit/CD117 (BD Biosciences; 550412) and PE-Cy7-conjugated anti-CXCR-4/CD184 (BD Biosciences ...
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bioRxiv - Cancer Biology 2024Quote: ... and stained with the following conjugated antibodies (1/100 dilution in PBS) for 15 minutes at 4°C: CD44 (PE, BD Biosciences 555479), EpCAM (FITC ...
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bioRxiv - Cell Biology 2022Quote: ... labelled with cell hashing antibodies and isolated by FACS (all steps at 4°C): Cell suspensions were incubated with Fc-block (1:50, BD Pharmingen 553142) for 10min and subsequently incubated with mouse CD45 MicroBeads (Miltenyi Biotec ...
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bioRxiv - Genomics 2023Quote: Trypsinized or MACS sorted cells were incubated at 4°C for 20 min with APC-conjugated anti-CD56 antibodies (1:40, BD Pharmingen #555518) or isotype control (1:40 ...
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bioRxiv - Cell Biology 2023Quote: The following antibodies were used in this study: rabbit anti-AP-4 epsilon 1:400 for Western blots (612019; BD Transduction Labs); rabbit anti-ATG9A 1:200 for immunofluorescence and 1:1000 for Western blots (ab108338 ...
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bioRxiv - Bioengineering 2024Quote: ... A third of each cell sample was stained for 30 mins at 4°C with 1:100-dilutions of APC-conjugated anti-C-Kit/CD117 (BD Biosciences; 550412) and PE-Cy7-conjugated anti-CXCR-4/CD184 (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: Splenocytes from infected mice were incubated for 5 hours at 37°C 5%CO2 in cRPMI prior intra-cytoplasmic detection of active-caspase 3 (BD Bioscience) using BD Fixation/Permeabilization kit (BD Bioscience) ...
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bioRxiv - Molecular Biology 2024Quote: The thymidine analog 5-bromo-2’-deoxyuridine (BrdU; BD Biosciences 550891) was used to assess cell proliferation in vitro ...
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bioRxiv - Systems Biology 2023Quote: ... Each row of a 96-well plate is a single BD construct (For example, row A: BD construct #1, row B: BD construct #2, row C: BD construct #3… to row H: BD construct #8) and each column is a single AD construct (For example column 1 ...
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bioRxiv - Systems Biology 2021Quote: ... Cells were then left at 4°C overnight in a blocking solution containing the primary antibody: mouse E-Cadherin (BD Biosciences, 1:1000) and mouse N-Cadherin (BD Biosciences ...
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bioRxiv - Neuroscience 2020Quote: ... Fixed cells were incubated overnight at 4 °C with primary mouse monoclonal anti-alpha synuclein antibody (Cat no. 610787; BD Biosciences; 1:200) and for 2 hr with secondary goat anti-mouse Alexa Fluor Plus 488 antibody (Cat no ...
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated in the FC wash buffer (2h at 4 °C) containing FITC-coupled anti-CD44 antibody (1 to 80 BD Pharmingen Cat.No. 555478) or its isotype control (FITC Mouse IgG2b ...
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bioRxiv - Neuroscience 2020Quote: Brains from paraformaldehyde-perfused 12 month-old mice were sectioned and 30 µm free-floating sections were incubated overnight at 4°C with primary antibodies (anti-αsyn, BD Transduction, 1:700), anti-DNAJC5 (Millipore ...
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bioRxiv - Immunology 2020Quote: ... Cells were then spun down at 500 × g for 5 min at 4°C and resuspended with the following antibody-conjugated fluorophores for 20 minutes at room temperature: anti-CD3-BV421 (BD, 1F4, 1:100), anti-CD4-PE-Cy7 (BD ...
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bioRxiv - Neuroscience 2023Quote: ... Homogenized tissue was washed with 3-4 ml washing buffer which was fresh PBSB 1% and then was filtered through a 70-µm cell strainer (BD Bioscience, NY, USA). Then using a long tube ...
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bioRxiv - Immunology 2024Quote: ... Cells were then washed and incubated in following antibodies for 30 minutes at 4°C in the dark: PerCP rat anti-mouse CD45 (557235, BD Biosciences, 1:50), FITC rat anti-CD11b (553310 ...
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bioRxiv - Synthetic Biology 2024Quote: ... cell samples were stained with the following antibodies in cell staining buffer for 40 min at 4 °C: anti-mouse-CD3e-FITC (553061, BD Biosciences, 1:200), anti-mouse-CD4-PercpCy5.5 (550954 ...
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bioRxiv - Immunology 2024Quote: ... TCR-engineered T cells containing 5×104 transgenic TCR (mTRBC)+ cells were co-cultured with 5×104 peptide-pulsed K562 cells for 4 h at 37°C in cRPMI and in the presence of 1 μL/mL GolgiPlug™ (BD Biosciences, 555029). As a positive control ...
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bioRxiv - Bioengineering 2024Quote: ... for 60 min at room temperature and then stained with anti-human/mouse phospho-STAT3-AF647 (clone 4/P-STAT3, BD Biosciences, 1:100) or anti-human/mouse phospho-SMAD2/3-AF647 (clone 072-670 ...
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bioRxiv - Bioengineering 2024Quote: ... 1:20 BD Pharmingen™ Alexa Fluor® 488 Mouse anti-SSEA-4 Clone MC813-70 (RUO) (BD Biosciences, Franklin Lakes, NJ, USA) 2 ml/well solution ...
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bioRxiv - Neuroscience 2023Quote: ... α-Syn (1:2 000, BD Biosciences ...
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bioRxiv - Microbiology 2020Quote: ... Cells were fixed with 4% paraformaldehyde in PBS and Click-iT chemistry (BD Pharmagen) performed according to the manufacturer’s instructions to fluorescently mark EdU+ cells.
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bioRxiv - Immunology 2021Quote: ... anti-IgD-BV510 antibodies then sorted at 4°C with FACS Aria (BD Bioscience). Post-sort purity was checked ...
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bioRxiv - Microbiology 2021Quote: ... Cells were fixed with 4% paraformaldehyde in PBS and Click-iT chemistry (BD Pharmagen) performed according to the manufacturer’s instructions to fluorescently mark EdU+ cells ...
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bioRxiv - Cancer Biology 2020Quote: ... for 4 hrs in the presence of 1μg/ml GolgiPlug™ (BD Pharmingen, 555029) as previously described10 ...
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bioRxiv - Immunology 2022Quote: ... for 45 minutes at 4°C after processing with Fixation/Permeabilization kit (BD Biosciences) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... and 4 mg/mL Difco™ Adjuvant (Mycobacterium Tuberculosis H37; BD Biosciences, Cat # 231141). Daily clinical scores were given as follows ...
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bioRxiv - Immunology 2022Quote: ... Then the cells were blocked with 4% FBS and anti-CD16/CD32 (BD Biosciences), incubated with surface marker antibodies for 20 minutes at 4° C and then permeabilized with BD Cytofix/Cytoperm buffer before intracellular labeling antibodies were added for 30 minutes at 4° C ...
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bioRxiv - Immunology 2022Quote: ... then were stained with PE conjugated pSTAT3 (pY705) (BD Bioscience clone 4/P-STAT3).
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bioRxiv - Microbiology 2021Quote: ... IL-10 and IL-4 were quantified according to ELISA kit instructions (BD Biosciences).
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bioRxiv - Genetics 2020Quote: ... Cells were then washed twice with PBS and fixed with 4% paraformaldehyde (BD Biosciences) for 30 min ...
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bioRxiv - Immunology 2021Quote: ... incubated for 30 min at 4°C in Phosflow Perm Buffer III (BD Biosciences), washed again ...
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bioRxiv - Genomics 2021Quote: ... for 20 min at 4°C and washed using Perm/Wash Buffer (BD Biosciences). Staining with primary antibodies was performed overnight at 4°C followed by incubation with suitable secondary antibodies diluted in Perm/Wash Buffer (Supplementary Table 4) ...
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bioRxiv - Genomics 2021Quote: ... Cells were then fixed in 4% PFA and quantified using flow cytometry (BD LSRFortessa). To test the sensitivity of the late gene reporter-infected lines ...
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bioRxiv - Neuroscience 2022Quote: ... Sorting was performed at 4°C in a FACSAria II cell sorter (BD Biosciences) using a 130 μm nozzle.
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bioRxiv - Cell Biology 2022Quote: ... washed with Stain Buffer and stained for p-STAT3 (clone: 4/P-STAT3, BD). Data was acquired on a BD Fortessa instrument and analysed using FloJo software ...
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bioRxiv - Immunology 2022Quote: ... CD33-BV421 (HIM3-4) and CD123-APC (7G3) (BD Biosciences, Franklin Lakes, NJ, USA). A FACSCanto™-II flow cytometer equipped with FACSDiva™ software (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... permeabilized with Perm Buffer III for 30 min at 4°C (both BD Biosciences), washed twice ...
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bioRxiv - Neuroscience 2022Quote: ... FACS sorted GFP fluorescent cells were mixed with 4% chilled Matrigel (v/v; BD Biosciences ...
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bioRxiv - Cancer Biology 2024Quote: ... plasma was collected using 21G needles into 4 ml EDTA Vacutainer tubes (BD, 367839), then centrifuged at 800 x g for 5 min at 4°C to remove cells ...
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bioRxiv - Microbiology 2023Quote: ... and PE-Cy™7 Rat Anti-Mouse IL-4 (560699, BD Pharmingen, USA). Flow cytometry data were acquired using an Aurora™ Flow Cytometer (Cytek ...
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bioRxiv - Cancer Biology 2023Quote: ... Biolegend)32 and by Western blotting (4-12% SDS-PAGE, clone JS-81, BD Biosciences). The FCM antibody panel and gating strategy are detailed in Supplemental Figure 1.33 CD81 mRNA was quantified by Taqman RT-PCR and GAPDH normalized (CD81 ...
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bioRxiv - Microbiology 2023Quote: ... Cells were fixed with 4% paraformaldehyde in PBS and Click-iT chemistry (BD Pharmagen) was performed according to the manufacturer’s instructions to fluorescently mark EdU+ cells.
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bioRxiv - Cell Biology 2023Quote: ... the membrane was incubated overnight at 4°C with mouse anti-dynactin p50 (BD Transduction Laboratories ...
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bioRxiv - Cell Biology 2024Quote: ... and incubated for 15 min.at 4 °C in Human BD Fc Block (BD Biosciences) to inhibit non-specific antibody binding ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the cells were blocked with 4% FBS and anti-CD16/CD32 (553141, BD Biosciences), incubated with surface marker antibodies for 20 minutes at 4°C and then permeabilized with BD cytofix/cytoperm buffer (554714 ...
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bioRxiv - Immunology 2024Quote: ... and Monensin protein transport inhibitors (4 µ L/6E6 cells; GolgiStop, BD Biosciences, 554724) and incubated for 4 hours at 37°C ...