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Citations for Becton, Dickinson and Company :
451 - 500 of 584 citations for 3 3 Bromomethyl phenyl thiophene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... 1 mL plastic syringes (BD PlastipakTM; sterile needles, 25G x 1” – NR. 18, 0.5 mm x 25 mm, BD MicrolanceTM 3), and portex tubing PE (Scientific Laboratory Supplies ...
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bioRxiv - Bioengineering 2020Quote: ... cells were then suspended in alginate precursor solution ‘A’ at 5 x 106 cells/mL (final concentration 2.5 x 106 cells/mL) and loaded into 1 or 3 mL syringes (BD luer lock). Following previous protocols ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were washed with PBS containing 5 mM EDTA and 3% FBS (FACS Buffer) and then blocked with 1% anti-mouse CD16/CD32 (BD biosciences), 5% normal rat serum and 5% normal mouse serum in FACS buffer for 10 minutes at RT ...
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bioRxiv - Plant Biology 2021Quote: ... A 1.0 mL aliquot consisting of approximately 108 cells was inoculated in a 100 mL 3% tryptic soy broth (TSB, BD-Difco) in Erlenmeyer flasks ...
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bioRxiv - Microbiology 2020Quote: ... AU) of the infected cells 1 and 3 hours after infection by flow cytometry using a FACS Aria III (BD Biosciences). The intensity of GFP fluorescence corresponds to the amount of intracellular bacteria ...
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bioRxiv - Immunology 2021Quote: ... Intracellular staining using antibodies detecting Active Caspase-3 (C92-605) and BCL2L11 (Y36) was performed using BD Cytofix/Cytoperm (BD Biosciences) or eBioscience FoxP3/Transcription Factor Staining Buffer Set (ThermoFisher) ...
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bioRxiv - Microbiology 2021Quote: ... 20µL were transferred into 180µL of 10mM Tris-1mM EDTA (for DiOC2(3) staining) or PBS (for DiSC3(5) staining) and analyzed by flow cytometry using LSR Fortessa (BD Bioscience) operated by FACS Diva software (BD Bioscience) ...
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bioRxiv - Plant Biology 2021Quote: ... Samples were run for 5 min at low flow rate (12 ± 3 μl min−1) and cytometric data were documented using BD CellQuest Pro software (BD Biosciences). In addition to glaucophyte cells ...
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bioRxiv - Cancer Biology 2021Quote: ... A total of 3 million MDA-MB-231-GFP-Luc cells were suspended in the mixture of PBS: Matrigel (BD Biosciences) at 1:1 ...
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bioRxiv - Immunology 2022Quote: ... ex vivo-stimulated cells were stained with cell surface markers followed by staining with anti-cleaved caspase-3 antibody (C92-605; BD Biosciences) for 45 minutes at 4°C after processing with Fixation/Permeabilization kit (BD Biosciences ...
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bioRxiv - Cell Biology 2022Quote: ... and plated on the upper chamber of the 24-well format HTS FluoBlokTM Multiwell Insert System (3 μm pore size; BD Falcon) in RPMI without phenol red (Life Technologies ...
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bioRxiv - Immunology 2020Quote: ... with the relevant peptides (each at 1 μg/ml) in the presence of anti-CD28 (clone L293) and anti-CD49d (clone L25) (each at 3 μl/ml; BD Biosciences). Cells were analyzed by flow cytometry after incubation for 24 hr at 37°C ...
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bioRxiv - Immunology 2021Quote: ... the wells were washed six times with DPBS containing 0.05% (v/v) Tween-20 (PBS-T 0.05%) and blocked with 3% (w/v) Difco Skim Milk (cat. n° 232100, BD Biosciences, USA) in PBS-T 0.05% for 2 h in agitation at RT ...
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bioRxiv - Cancer Biology 2020Quote: ... and the fraction of sgRNA-expressing cells was measured 3 days and 5 days after treatment as BFP-positive cells by flow cytometry on an LSR-II (BD Biosciences). Specifically ...
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bioRxiv - Immunology 2021Quote: ... the wells were washed six times with PBS containing 0.05% (v/v) Tween-20 (PBS-T) and blocked with 3% (w/v) Difco Skim Milk (BD Biosciences, USA) in PBS-T for 2 h in agitation at RT ...
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bioRxiv - Immunology 2020Quote: ... followed by staining for 3 days at 37 °C with 1:100 dilutions of BV421-labeled mouse anti-human CD35 clone E11 (BD Biosciences) and Alexa Fluor 488-labeled mouse anti-Ki67 clone B56 (BD Biosciences ...
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bioRxiv - Cancer Biology 2021Quote: ... the samples were rinsed 3 times in FACS buffer and then suspended in FACS buffer for analysis and sorting with FACSARIA III (BD Biosciences). Living single cells were selected via FSC/SSC isolation.
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bioRxiv - Cancer Biology 2022Quote: ... Cells were analysed on the BD LSR Fortessa X-20 or BD FACSymphony or sorted on the BD FACSAria Fusion 5 or BD FACSAria Fusion 3 (BD Biosciences). Data was analysed using FlowJo (v 10.4).
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bioRxiv - Immunology 2022Quote: Igλ-enriched B1-8 cells were cultured for 2-3 days at 4 *105 cells/well in a 12-well tissue culture plate (BD Falcon) and then washed ...
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bioRxiv - Microbiology 2020Quote: ... The concentration of iron in LB was calculated using specifications provided in the “Bionutrients Technical Manual Vol 3” supplied by the manufacturer (BD Biosciences). For assays testing the role of iron availability ...
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bioRxiv - Microbiology 2020Quote: ... NJ) were coated for 3□hours with 10□μg of Matrigel following the manufacturer’s instructions (Biocoat, BD Biosciences, San Jose, CA). Inserts were placed into 24-well dishes containing 500□μl of RPMI medium supplemented with 1% fetal calf serum ...
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bioRxiv - Microbiology 2021Quote: Cells were harvested using trypsin and washed with PBS 3 times gently and then stained with propidium iodide (BD Bioscience, 556547) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... for 30 min prior to the addition of unconjugated anti-CD28 (clone L293) and anti-CD49d (clone L25) (each at 3 μl/ml; BD Biosciences), brefeldin A (1 μl/ml ...
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bioRxiv - Immunology 2020Quote: ... in the presence of unconjugated anti-CD28 (clone L293) and anti-CD49d (clone L25) (each at 3 μl/ml; BD Biosciences) and IL-2 (10 IU/ml ...
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bioRxiv - Neuroscience 2021Quote: ... Purified bone marrow cells were centrifuged at 1500 rpm for 3 min and filtered through a 100 μm cell strainer (BD Biosciences). The bone marrow cells were plated onto a 100 mm culture dis h or six-well plate (BD Biosciences ...
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bioRxiv - Pathology 2021Quote: ... 0.2 micron sterile filtered) using an insulin syringe (31-gauge, 6 mm length, 3/10 cc, BD Veo Ultra-Fine needle). Glucose and lactate levels were measured using a minimally invasive tail-prick method at designated timepoints post-injection.
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bioRxiv - Immunology 2021Quote: ... NK cells purity at the end of the 3-week coculture was (> 90%) as determined by flow cytometry (BD Accuri C6) using CD56 (5.11H11 ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained with cell phenotyping antibodies in a 1:1 volume ratio of 3% FBS and Brilliant Stain Buffer (BD Biosciences) according to standard procedures ...
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bioRxiv - Cell Biology 2023Quote: ... After 1 h of incubation at 37 °C the cells were washed 3 times with HBSS followed by trypsinization and signal was measured using FACScan and FACSCalibur systems (BD Biosciences). Data was analyzed with FlowJo™ v10 Software (BD Biosciences).
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bioRxiv - Cancer Biology 2023Quote: ... Flow cytometry was performed on a 3 laser Cytek Aurora Cytometer (Cytek Biosciences, Fremont, CA) and analyzed using FlowJo software (BD Biosciences). All antibodies are from Biolegend ...
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bioRxiv - Immunology 2023Quote: ... Pooled cells from n = 3 - 5 mice/cohort were stained with PE- or APC-conjugated antibodies (from either BD or BioLegend) against murine F4/80 (clone ...
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bioRxiv - Immunology 2023Quote: ... with 3×104 PFU or injected intracranially (10uL) with 50 PFU of LGTV-TP21 using insulin syringes (BD Medical, #BD-329461). At appropriate times post infection ...
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bioRxiv - Physiology 2022Quote: ... Sections were washed 3 times in wash buffer then stained for Alexa Fluor 555 mouse anti-E-cadherin (BD, Cat. 560064) for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... Confluent definitive endoderm cells are then passed 1:3 in presence of Rock Inhibitor on growth factors reduced Matrigel (BD Biosciences) and cultured for 8 days in differentiation medium (DMEM F12 ...
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bioRxiv - Biochemistry 2023Quote: ... 1 µg of this genomic DNA from soluble fraction was mixed with 3 µg of anti-IdU/BrdU antibody (3D4 clone, BD Science) in BrdU-IP buffer composed of PBS and 0.0625% Triton X-100 overnight ...
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bioRxiv - Cell Biology 2024Quote: Cells were seeded 24 h before treatment and were allowed to grow in the presence of 3 μg/mL BrdU (BD Pharmingen) for 48 h ...
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bioRxiv - Immunology 2024Quote: ... and 5×104 of each were combined in 96-well U-bottom plates with anti-CD40 (100 ng/mL, clone HM40-3, BD Biosciences) in the presence or absence of OVA (10 μg/mL ...
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bioRxiv - Immunology 2023Quote: ... and ionomycin (500 ng/mL) for 3 hrs at 37°C prior to surface and intracellular staining using the BD Cytofix/Cytoperm Kit (BD Biosciences). Intracellular transcription factor staining was performed using a Foxp3 staining kit (ThermoFisher) ...
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bioRxiv - Cancer Biology 2023Quote: ... Flow cytometry was performed on a 3 laser Cytek Aurora Cytometer (Cytek Biosciences, Fremont, CA) and analyzed using FlowJo software (BD Biosciences). All antibodies are from Biolegend unless otherwise specified.
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bioRxiv - Immunology 2023Quote: ... and 1:20 dilution mouse anti-human CD123 (IL-3)-PE (stock 200 ug/mL, BD Biosciences, Franklin Lakes, NJ, USA). A 1:1,000 dilution of anti-mouse IgG Alexa-647 (stock 1 mg/mL ...
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bioRxiv - Microbiology 2023Quote: ... cells were incubated with fluorochrome-conjugated monoclonal antibodies (mAbs) diluted at 1:200 into a 1:3 solution of Brilliant Staining Buffer (BD 563794): Cell Staining Buffer ...
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bioRxiv - Cell Biology 2023Quote: ... The cells followed by an incubation with the SM/Chol probe OlyA (3 µM) and primary anti-IFN-γR1 antibody (1:50) (BD Biosciences) together in 1% BSA-PBS at room temperature for 90 min ...
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IpaA reveals distinct modes of vinculin activation during Shigella invasion and cell-matrix adhesionbioRxiv - Cell Biology 2023Quote: ... NJ) were coated for 3 hours with 10 μg of Matrigel following the manufacturer’s instructions (Biocoat, BD Biosciences, San Jose, CA). Inserts were placed into 24-well dishes containing 500 μl of RPMI medium supplemented with 1% fetal calf serum ...
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bioRxiv - Evolutionary Biology 2023Quote: ... After 48h cultures were diluted and plated on Nutrient Agar plates (5 g/L peptone BD difco, BD Bioscience; 3 g/L yeast extract BD difco, BD Bioscience, 15 g/L agar Bacto, BD Bioscience). Plates were left at room temperature until colonies were detectable and distinguishable (2-4 days) ...
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bioRxiv - Plant Biology 2024Quote: ... The dry volume (DV, m3) was measured by water displacement (Berta et al., 2010) and basic density (BD, kg m-3) calculated as reported by Carriero et al ...
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bioRxiv - Systems Biology 2024Quote: ... freshly sorted lineage-labeled Dclk1+ cells were resuspended in LPM-3D medium (LPM-3) and mixed with growth factor-reduced Matrigel (BD Biosciences) in a 1:1 ratio ...
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bioRxiv - Immunology 2022Quote: Splenocytes from infected mice were incubated for 5 hours at 37°C 5%CO2 in cRPMI prior intra-cytoplasmic detection of active-caspase 3 (BD Bioscience) using BD Fixation/Permeabilization kit (BD Bioscience) ...
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bioRxiv - Microbiology 2022Quote: ... in PBS at 37°C for 30 mins followed by one wash with PBS at 450 g for 3 mins and finally resuspend in 100 μl PBS for analysis using flow cytometry (BD Fortessa).
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bioRxiv - Microbiology 2022Quote: ... Cells were harvested and washed with 3 x with PBS before performing FACS using a BD FACS Celesta Cell Analyzer (BD Biosciences).
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bioRxiv - Cancer Biology 2022Quote: ... Percentage of caspase-3/7 negative cells in each treatment condition was normalized to vehicle-treated control populations (FlowJo software v9, BD Biosciences).