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Citations for Becton, Dickinson and Company :
451 - 500 of 8606 citations for 2 5 Sulfanyl 4H 1 2 4 triazol 3 yl phenol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: HCC1806 cells were starved in serum-free media for 4h before seeding 200,000 cells in serum free media on top of an 8-μm PET membrane transwell (BD-Biosciences) in a 24-well format and allowed to migtrate into the lower compartment containing media supplemented with 15% of FBS for 24 hours ...
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bioRxiv - Genetics 2024Quote: ... Following blocking (5% milk/BSA) membranes were incubated with primary antibody overnight at 4°C (BIP [1:40,000 BD Transduction 610979] ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were rinsed with 2 × 2 ml Pharmingen stain buffer and fixed with 4.21% (w/w) formaldehyde (BD Biosciences, #554655). Cells were analyzed on LSRFortessa X-20 (BD Biosciences ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed with ice-cold FACS buffer (DPBS supplemented with 2% FBS and 2 mM EDTA) and subjected to fixation and staining with Cell Fixation and Permeabilization Kits (BD Biosciences). Incubation with antibodies was performed for 1h at RT ...
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bioRxiv - Immunology 2023Quote: ... All flow cytometry analyses were performed in FACS buffer containing PBS with 2 mM EDTA and 2% FBS on a FACS CANTO (BD Pharmingen) and analysed with FlowJo software (Treestar).
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bioRxiv - Microbiology 2023Quote: ... Membranes were blocked with 2% ECL prime blocking reagent in PBS-T (Amersham; #RPN418) and incubated against AP-2 (mouse-anti-AP50, BD, #A611351) and GAPDH (rabbit-anti-GAPDH ...
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bioRxiv - Immunology 2023Quote: ... Samples were stored in PBS containing 2% (V/V) FBS and 2 mM EDTA until acquisition on an LSR Fortessa flow cytometer (BD Biosciences). Analyses were performed with FlowJo v10.7.1 (BD Biosciences).
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bioRxiv - Microbiology 2023Quote: ... and values for IL-2 were determined by using a standard curve generated by using known concentrations of purified recombinant murine IL-2 (BD Biosciences).
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bioRxiv - Immunology 2023Quote: ... up to 2 x106 MR1-5-OP-RU-APC Tetramer magnetic bead enriched spleen (n= 2) or unenriched colon (n= 2) single cell suspensions were labeled with an oligonucleotide-tagged antibody sample tag (BD Biosciences). MAIT cells (MR1-5-OP-RU-Tet+TCRβ+CD3+ ...
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bioRxiv - Biophysics 2023Quote: ... Cells were then washed twice in FACS buffer (PBS, 2% FBS, 2 mM EDTA) and permeabilized in BD Phosflow Perm Buffer III (BD Biosciences), according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... IL-2 levels in the co-culture supernatant were quantified using ELISA (BD OptiEIA Human IL-2 ELISA kit, BD Biosciences) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... resuspended in 300 μL flow buffer (1X PBS/FBS 2 %, 2 mM EDTA) and subjected flow cytometry analysis (Sony, SH800S Cell Sorter or BD FACSAria). Cell cycle analysis of vital mCherry-dCas9 and GFP-sgRNA expressing double positive cells was done using FlowJo SoftwareTM.
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bioRxiv - Immunology 2023Quote: ... 5 μg/mL of anti-IL-4 (BD Pharmingen, REF: 554385) and 10 ng/mL of IL-2 (R&D systems ...
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bioRxiv - Neuroscience 2022Quote: ... Daigger Scientific) with a rounded tip connected to a blunted 30G x 1/2 needle (BD PrecisionGlide) and 1 cc syringe (Terumo ...
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bioRxiv - Neuroscience 2021Quote: Primary antibodies were obtained from the following sources: anti-ataxin-2 (mouse monoclonal, 1:500, BD biosciences) anti-ataxin-3 (mouse monoclonal 1H9 ...
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bioRxiv - Immunology 2024Quote: ... the following monoclonal antibodies were used: anti-Human CD253 (TRAIL)-PE (RIK-2, 1:100, BD Biosciences), anti-Hu CD95 (APO-1/Fas)-APC (DX2 ...
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bioRxiv - Immunology 2023Quote: ... modifications to the protocol were made: 1 μl of 2 μM additive primer (BD Genomics, beta kit) specific to the antibodies tags was added to the amplification mixture ...
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bioRxiv - Immunology 2023Quote: ... cells were washed 2x in FACS buffer (5% FBS, 2 mM EDTA in PBS) and resuspended with anti-CD16/CD32 Mouse BD FC Block (BD Biosciences, Cat. 553142) in FACS buffer at a concentration of 10 ug/ml for 15 min ...
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bioRxiv - Immunology 2021Quote: ... were cultured with 5E5 of YAC-1 target cells (2:1 ratio) in the presence of anti-mouse CD107a-BV421 antibody (564347, BD Biosciences) (1:100 ...
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bioRxiv - Cell Biology 2020Quote: Protein synthesis of Syndecan-1 was shown by immunocytochemistry using a polyclonal antibody against murine Syndecan-1 (CD138) clone 281-2 (1:50, rat anti-mouse, BD Pharmingen) in co-cultured cells after fixation with ice-cold 99% methanol and heparitinase III (2.4mIU/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... xenograft models were generated by injecting ES2-shARID1A cells [2 × 106 cells mixed 1:1 (v/v) with Matrigel (BD Biosciences)] subcutaneously ...
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bioRxiv - Immunology 2020Quote: ... PBMC (2 × 106 cells/well) were stimulated with anti-CD28 (1 μg/ml) and anti-CD49d (1 μg/ml) (FastImmune, BD Biosciences) and 1e5 M ...
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bioRxiv - Immunology 2024Quote: ... wells were incubated with 100 µL of biotinylated anti-IgG1 (1 µg/mL in PBS with 2% FBS, clone A85-1, BD Biosciences) for 2 hours at RT ...
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bioRxiv - Immunology 2023Quote: ... plates were incubated with serum (2 fold dilutions, 1:20-1:2560) and parasite specific antibody was measured using biotinylated IgG1 (BD Biosciences) or IgG2c (BD Biosciences ...
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bioRxiv - Bioengineering 2023Quote: ... TCR expressing cells were mixed with K562-A2 cells at a ratio of 1:2 (Effector:Target) in the RPMI media and supplemented with 1 µg/ml of anti-CD28/CD49d antibodies (BD Biosciences, 347690) and 1 µg/ml of cognate peptides ...
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bioRxiv - Cancer Biology 2024Quote: ... Either 3×106 cells for MiaPaca2 or 2×106 cells for BxPC3 were suspended in a 1:1 solution of PBS and Matrigel (BD Biosciences) and injected into the subcutaneous space of athymic nude mice (Invigo) ...
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bioRxiv - Synthetic Biology 2021Quote: ... Then cells were resuspended with primary antibodies anti-Oct-3/4 (BD, 611203) and anti-Sox2 (D6D9 ...
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bioRxiv - Immunology 2022Quote: ... the cells were fixed with 2% PFA and stored at 4°C until the samples were acquired on a LSRII cytometer (BD Biosciences, Mississauga, ON, Canada). Data analysis was performed using FlowJo v10.5.3 (Tree Star ...
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bioRxiv - Immunology 2020Quote: 2 × 106 cells were stimulated for 4 h at 23°C with 40 ng ml−1 PMA and 1 μg ml−1 ionomycin in the presence of 2 μM Monensin (Golgi-Stop, BD Biosciences, Cat: 51-2092KZ). Cells were washed twice with FACS buffer and stained for extracellular markers ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were washed with PBS containing 5 mM EDTA and 3% FBS (FACS Buffer) and then blocked with 1% anti-mouse CD16/CD32 (BD biosciences), 5% normal rat serum and 5% normal mouse serum in FACS buffer for 10 minutes at RT ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: SARS-CoV-2 infected cells were fixed in 2% formaldehyde for 30 mins and permeabilized with BD Perm/Wash buffer (BD Biosciences, 554723). The primary antibody used was sheep anti-SARS-CoV-2 nucleocapsid antibody (DA114 ...
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bioRxiv - Immunology 2021Quote: ... 50 U/ml Penicillin and 50 ug/ml Streptomycin) supplemented with 100 U/ml hIL-2 and 2 ug/ml anti-CD28 (BD Pharmingen, 553294). Two days after the primary stimulation ...
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bioRxiv - Immunology 2023Quote: ... were diluted 1:50 in PBS-10% FBS and IL-2 concentration was determined by ELISA assays using the OptEIA Human IL-2 ELISA commercial kit (BD Biosciences, USA) and revealed with TMB substrate (Thermofisher ...
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bioRxiv - Genetics 2024Quote: ... After 24 hours we selected the transfected cells either by puromycin treatment (2 ug/ml for 2 days) or flowcytometric sorting for double positive cells (BD FACSAriaTM fusion).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... for 5 min at 4 °C and immunostained with CD11b-AlexaFluor 647 (M1/70; 1:1000; Cat#557686, BD Biosciences) and CD45-PE (1:1000 ...
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bioRxiv - Physiology 2022Quote: ... Venous peripheral blood (4-6 ml for adults, 1- 3 mL for children and infants) was collected on-site on EDTA (BD vacutainer©) and immediately stored at 4-8°C until use ...
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bioRxiv - Cell Biology 2020Quote: ... 2 mL of dispase (BD Biosciences, 50 U/ml) was instilled into the lungs through the trachea until the lungs inflated ...
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bioRxiv - Immunology 2022Quote: ... TCR Vβ14-Biotin (BD Bioscience: 553257, clone: 14-2), Streptavidin-BV421 (BioLegend ...
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bioRxiv - Immunology 2021Quote: B cell panel: IgD-BUV395 (IA6-2, BD Horizon), CCR7-BV421 (G043H7 ...
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bioRxiv - Immunology 2021Quote: ... Cultures were supplemented with 10IU IL-2 (BD Biosciences) 2-3 times weekly ...
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bioRxiv - Bioengineering 2020Quote: ... 2% Bacto™-agar (BD, Franklin Lakes, NJ, USA) was added to make agar plates.
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bioRxiv - Microbiology 2021Quote: ... in Noble Agar (BD DIFCO™, 2% w/v), cooling to 50°C with agitation ...
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bioRxiv - Biochemistry 2021Quote: ... Cells resuspended in 2% BSA were analyzed by BD LSR Fortessa or LSRII SORP (Becton Dickinson) ...
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bioRxiv - Immunology 2022Quote: ... and ERK1/2 (pT202/pY204) were purchased from BD Biosciences ...
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bioRxiv - Genomics 2020Quote: ... Data were collected on LSRII or Fortessa 2 (BD) and were analyzed with FlowJo software (TreeStar).
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bioRxiv - Immunology 2020Quote: ... on SM agar plates [2% Bacto agar (BD difco) with SM media ...
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bioRxiv - Immunology 2022Quote: ... anti-CD28 (2 µg/ml, clone 37.51; BD Biosciences), and rmTGFβ (10 ng/ml ...
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bioRxiv - Immunology 2020Quote: ... TCR Vβ8.1/8.2 BUV395 (Clone MR5-2, BD Biosciences), TCRβ AF700 (Clone H57-597 ...
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bioRxiv - Genomics 2020Quote: ... 2 μl of ms BrdU-antibody (BD Bioscience, B44) (Control for unspecific binding to mouse antibodies) ...
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bioRxiv - Microbiology 2020Quote: ... fixed with 2% paraformaldehyde with Fc block (BD Biosciences) before and after ...