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Citations for NanoTemper :
51 - 61 of 61 citations for Recombinant Human Lipocalin 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: Binding of purified recombinant KIX with fluorescein isothiocyanate (FITC)-conjugated peptides was measured by microscale thermophoresis using the Monolith NT.115 (NanoTemper Technologies). Assays were conducted in 50 mM sodium phosphate ...
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bioRxiv - Biochemistry 2024Quote: The binding affinity of each recombinant TrK13 protein for heme was evaluated by MST analysis using the Monolith NT.115 instrument (NanoTemper Technologies). The recombinant TrK13 proteins (50 nM ...
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bioRxiv - Plant Biology 2022Quote: ... Thermal unfolding of G-actin (2 μM) and F-actin (2 μM) was performed with the Tycho NT.6 (Nanotemper, Munich, Germany) according to the manufacturer’s instructions in G-buffer (5 mM Tris-HCl ...
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bioRxiv - Cell Biology 2021Quote: ... Samples in the presence and absence of 2 mM ATP with 2 mM MgCl2 were loaded in high-sensitivity capillaries (Nanotemper, Munich, Germany) and heated with a 0.3°C/min temperature ramp from 20°C to 70°C with a 2 sec DLS integration time.
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bioRxiv - Biophysics 2024Quote: The (I91)2-ClfAN2N3-(I91)2 polyprotein was fluorescently labeled by utilizing the commercial protein Labeling Kit RED-NHS 2nd Generation (Nanotemper Technologies GmbH, Munich, Germany). The dye carries a reactive NHS-ester group that reacts with primary amines in the protein to form a covalent bond ...
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bioRxiv - Molecular Biology 2021Quote: ... between protein:compound sample and a protein only sample at 1.5-2-5 sec is calculated and plotted through MO.Affinity analysis v2.3 (NanoTemper Technologies) and GraphPad Prism 8.0.0 (GraphPad Software ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: The thermostability of MAPK14 was measured in the presence of different concentrations of MBZ in 50 mM HEPES buffer in the presence of 200 mM NaCl and 2% DMSO at pH 7.5 using a label-free fluorimetric analysis with a Prometheus NT.Plex instrument (NanoTemper Technologies), as described previously [52] ...
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bioRxiv - Biochemistry 2023Quote: ... was measured from 15°C to 95°C with a ramp rate of 2°C per minute with a Prometheus NT.48 nano-fluorimeter (NanoTemper). The melting temperatures (Tm values ...
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bioRxiv - Biophysics 2021Quote: The data was analyzed and the Ktet (Eq. 1) or EC50 (Eq. 2) calculated using the MO affinity analysis software (NanoTemper, Germany), considering a 1:1 model.
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bioRxiv - Molecular Biology 2020Quote: ... and Spike RBD mixed with W25 (2 μM each) were prepared and loaded into Capillaries Tycho NT.6 (TY-C001, NanoTemper Technologies GmbH). The Tycho instrument applied a quick thermal ramp from 35°C to 95°C with a heating rate of 30°C/min and the unfolding of proteins were monitored through changes in the 350 nm / 330 nm fluorescence emission ratio ...
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bioRxiv - Molecular Biology 2020Quote: ... between the W25 Nanobody and SARS-CoV-2 S1 of Spike RBD was measured by microscale thermophoresis (MST) using a Monolith NT.115PICO instrument (Nanotemper Technologies GmbH). Purified W25 was buffer exchanged into a PBS buffer ...