Labshake search
Citations for NanoTemper :
1 - 50 of 189 citations for Recombinant Human LDLR protein GST tagged since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2021Quote: Recombinant His-tagged proteins were labeled using the Monolith His-Tag Labeling Kit RED-tris-NTA (NanoTemper, MO-L008). In all experiments ...
-
bioRxiv - Biochemistry 2024Quote: ... The purified recombinant His-tagged PfSir2A was labeled by the RED-tris-NTA 2nd generation dye (NanoTemper Technologies). The protein concentration was adjusted to 200 nM in PBS-T buffer ...
-
bioRxiv - Neuroscience 2021Quote: ... Recombinant human FTO protein was labeled with His-tag using Monolith His-Tag Labeling Kit RED-tris-NTA (NanoTemper Technologies GmbH; MO-L008). The labelled FTO protein (target ...
-
bioRxiv - Biochemistry 2023Quote: ... Purified GST fusion protein were fluorescently labeled using the Monolith series Protein Labeling Kit RED-NHS 2nd Generation (Nanotemper, MO-L011) according to manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: His-tagged MLCK proteins were labeled using a His-Tag Labeling Kit RED-tris-NTA (NanoTemper). Microscale thermophoresis was performed using a Monolith NT.115 (NanoTemper) ...
-
bioRxiv - Cancer Biology 2024Quote: ... Purified recombinant PTPN2 protein (20 μM) was labeled according to the protein labeling kit RED-NHS (Nanotemper, Cat. No. L001). The K73 compound was diluted into 16 concentration gradients with 1xPBS-P using the fold-and-half dilution method ...
-
bioRxiv - Biochemistry 2021Quote: C-terminally His6-tagged proteins were labeled using the Monolith His-tag labeling kit RED-tris-NTA (Nanotemper #MO-L008) following the manufacturer’s protocol ...
-
bioRxiv - Developmental Biology 2023Quote: ... His-tagged RALF proteins were labeled with red dyes with the His-Tag labelling kit RED-tris-NTA 2nd Generation (NanoTemper) according to the manufacturers’ recommendation ...
-
bioRxiv - Molecular Biology 2024Quote: ... 200 µL of 100 nM solution of his-tagged protein was labeled with RED-tris-NTA 2nd Generation dye (Monolith HIS-tag labeling Kit, NanoTemper). Protein-protein complexes were obtained by mixing dye-labeled IFIT protein (final concentration 50 nM ...
-
bioRxiv - Molecular Biology 2022Quote: ... we evaluated the interaction of recombinant PfPHB1 and PfPHB2 proteins using Monolith NT.115 instrument (NanoTemper Technologies, Munich, Germany). Thermophoretic movement of a fluorescently labelled protein is measured by monitoring the fluorescence distribution ...
-
bioRxiv - Genetics 2024Quote: Recombinant IRF6 protein was labeled using the Monolith His-Tag Labeling Kit RED-tris-NTA (Nanotemper Technologies, MO-L018) according to the manufacturer’s protocol at a 1:2 dye:protein ratio ...
-
bioRxiv - Biochemistry 2024Quote: The human proteasomes were labelled using the Monolith NT™ Protein Labeling Kit (NanoTemper) and exchanged to reaction buffer (50 mM Tris-HCl [pH 7.5] ...
-
bioRxiv - Biochemistry 2024Quote: The binding affinity of each recombinant TrK13 protein for heme was evaluated by MST analysis using the Monolith NT.115 instrument (NanoTemper Technologies). The recombinant TrK13 proteins (50 nM ...
-
bioRxiv - Microbiology 2022Quote: Purified WT or mutant NSP12 recombinant proteins were labeled with Cytidine-5 dye using the Monolith His-Tag labeling kit (NanoTemper Technologies), and then labeled NSP12 proteins were diluted to 700 nM in PBST buffer ...
-
bioRxiv - Biochemistry 2020Quote: ... histidine tagged HMGB1 was labeled by the his-tag specific NT-647 dye (Monolith NTTM Protein Labelling Kit RED-NHS, NanoTemper® Technologies GmbH, Mu□nchen, Germany), for 30 minutes at room temperature ...
-
bioRxiv - Biochemistry 2024Quote: ... containing 6.25 μM of His-tagged PyrE labeled with RED dye (Nanotemper Technologies, USA), with an equal volume of the respective ligand solution ...
-
Cryo-EM structure of a eukaryotic zinc transporter at a low pH suggests its Zn2+-releasing mechanismbioRxiv - Biochemistry 2022Quote: ... by staining His-tagged xtZnT8 with the RED-Tris-NTA 2nd Generation dye (NanoTemper Technologies, Germany). For Zn2+ titration ...
-
bioRxiv - Biochemistry 2022Quote: His6-tagged caspases were labelled using the His-tag labelling Kit RED-tris-NTA 2nd generation (NanoTemper). Briefly ...
-
bioRxiv - Biochemistry 2023Quote: 300 nM 6His-tagged EF-Tu was incubated with 75 nM Red Tris NTA dye Generation 2 (NanoTemper Technologies) in binding buffer (287 mM NaCl ...
-
bioRxiv - Biochemistry 2024Quote: Site-specific labelling of His-tagged BRISC was performed using a RED-tris-NTA 2nd generation labelling kit (NanoTemper Technologies) in buffer containing 25 mM HEPES pH 7.5 ...
-
bioRxiv - Cell Biology 2020Quote: ... his-tagged TLNRD1 was diluted to 100 nM and coupled with His-tag NT-647 dye (RED-tris-NTA NanoTemper, München, Germany) at room temperature for 30 min ...
-
bioRxiv - Microbiology 2024Quote: The protein-DNA and protein-protein interaction were assayed by microscale thermophoresis (MST) using Monolith NT.115 (NanoTemper Technologies, Germany) as described earlier (Su et al ...
-
bioRxiv - Microbiology 2021Quote: Proteins were labeled using the Monolith NT Protein Labeling Kit RED (NanoTemper Technologies) according to the manufacturer’s protocol and using a buffer consisting of 20 mM HEPES ...
-
bioRxiv - Molecular Biology 2021Quote: ... EMCV 2A protein was labelled using the Protein Labelling Kit RED-Maleimide (NanoTemper Technologies) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2024Quote: Target proteins were fluorescently labeled using the Protein Labeling Kit RED-NHS (NanoTemper Technologies). Labeling reaction was performed following the manufacturer’s instructions with 10 μM protein ...
-
bioRxiv - Cell Biology 2020Quote: Protein-protein interactions were monitored by MST using a Monolith NT.115 instrument (Nanotemper Technologies). His6-MyoVa GTD was labeled using RED-NHS 2nd Generation (Amine Reactive ...
-
bioRxiv - Cell Biology 2020Quote: ... Proteins were labeled using the RED-tris-NTA His tag protein labeling kit (Nanotemper Technologies). Labelled protein was added to a serial dilution of unlabeled DAG ...
-
bioRxiv - Cancer Biology 2023Quote: ... EED and JMJD3 proteins were labeled with Protein Labeling kit RED-NHS (NanoTemper, MO-L001). At least three independent experiments were performed for each binding reaction.
-
bioRxiv - Cell Biology 2022Quote: ... Proteins were labeled using the RED-tris-NTA His tag protein-labeling kit (Nanotemper Technologies). Labeled proteins were added to a serial dilution of unlabeled DAG or liposomes prepared in binding buffer (20 mM Tris ...
-
bioRxiv - Biophysics 2024Quote: The melting temperatures (Tm) of recombinant VDAC were determined by native tryptophan fluorescence using a Tycho Differential Scanning Fluorometer (Nanotemper). VDAC samples were diluted to 1mg/ml in 25 mM HEPES at pH 7.4 ...
-
bioRxiv - Biochemistry 2022Quote: Protein–protein interactions were monitored by microscale thermophoresis using a Monolith NT.115 instrument (Nanotemper Technologies). His LRRK2 Armadillo (1-552) ...
-
bioRxiv - Biochemistry 2023Quote: Protein–protein interactions were monitored by microscale thermophoresis using a Monolith NT.115 instrument (NanoTemper Technologies). His LRRK2 Armadillo (1–552 ...
-
bioRxiv - Biophysics 2023Quote: ... where the protein was labelled with Monolith Protein labelling kit RED-NHS (NanoTemper Technologies, Munich, Germany). In the binding assay ...
-
bioRxiv - Microbiology 2022Quote: ... Protein-protein interactions were analyzed at 20°C by MST on a Monolith NT.115 (NanoTemper technologies) using standard capillaries ...
-
bioRxiv - Microbiology 2023Quote: ... Proteins were fluorescently labeled using the RED-Maleimide 2nd Generation Protein Labeling Kit (NanoTemper Technologies GmbH, Germany) as recommended by the manufacturer ...
-
bioRxiv - Physiology 2023Quote: ... Purified OsABG protein was labeled using the protein labeling kit Red-NHS 2nd generation (MO-L011, Nanotemper) which dyes primary lysine residues in the protein ...
-
bioRxiv - Cancer Biology 2021Quote: Binding of purified recombinant KIX with fluorescein isothiocyanate (FITC)-conjugated peptides was measured by microscale thermophoresis using the Monolith NT.115 (NanoTemper Technologies). Assays were conducted in 50 mM sodium phosphate ...
-
bioRxiv - Molecular Biology 2023Quote: The affinity between RNA and protein and between proteins was measured by MST on a Monolith NT.115 (NanoTemper, Germany). Replicates represent individual measurements performed on different days using proteins from the same prep ...
-
bioRxiv - Biochemistry 2020Quote: ... His-tagged protein (Pry1 or Na-ASP-2) was fluorescently labeled using the RED-tris-NTA His tag protein labeling kit (Nanotemper Technologies). Labeled protein (Pry1 or Na-ASP-2 ...
-
bioRxiv - Microbiology 2021Quote: ... Phr2 and Pga4 proteins and Bull Serum Albumin (BSA) were labeled using the Monolith™ RED-NHS Protein Labeling Kit (NanoTemper Technologies) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... The protein interaction was measured in standard capillaries (NanoTemper), 100% excitation power and medium or high MST power (IR-laser intensity) ...
-
bioRxiv - Immunology 2022Quote: Protein-protein interactions were analyzed via microscale thermophoresis on a Monolith NT.115 instrument equipped with green/red filters (NanoTemper Technologies, Munich, Germany). Measurements were performed at 25°C at both 40% and 80% MST power ...
-
bioRxiv - Biochemistry 2020Quote: ... Thermally-driven protein unfolding was monitored in Tycho quartz capillaries (Nanotemper) using a Tycho NT.6 instrument (Nanotemper) ...
-
bioRxiv - Microbiology 2022Quote: ... Protein quality was verified using a Tycho NT.6 system (NanoTemper) and ACE2 activity assay kit (SensoLyte ...
-
bioRxiv - Biophysics 2024Quote: ... CRBN protein was labelled with RED-Maleimide 2nd Generation dye (NanoTemper) optimized for degree-of-labeling of 1:1 molar ratio calculated by nanodrop ...
-
bioRxiv - Immunology 2023Quote: ... Protein samples (0.1 mg/mL) were loaded into glass capillaries (NanoTemper) and then subjected to a temperature gradient from 20 to 95°C at a heating rate of 1°C per min ...
-
bioRxiv - Biophysics 2024Quote: ... Protein Labeling Kit RED-NHS 2nd Generation was purchased from NanoTemper Technologies (München ...
-
bioRxiv - Cell Biology 2023Quote: ... Monolith RED-NHS 2nd generation protein labeling kit (NanoTemper Technologies GmbH) was used to label LIAS and ...
-
bioRxiv - Immunology 2022Quote: ... Protein samples (0.1 mg/mL) were loaded into glass capillaries (NanoTemper) and then subjected to a temperature gradient from 20 to 95 °C at a heating rate of 1 °C per min ...
-
bioRxiv - Biophysics 2021Quote: ... or Monolith NT™ (Protein Labeling Kit Blue-NHS, NanoTemper GmbH, Germany). The labeling was achieved by incubating 115 μM of protein and 350 μM of dye (465 μl reaction volume ...