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Citations for NanoTemper :
51 - 100 of 125 citations for Recombinant Human Interleukin 3 Receptor Alpha Low Affinity His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: His-Tag labeling Kit RED-tris-NTA 2nd generation (MO-L018, Nanotemper) was used to label His-tagged Bax purchased from Creative BioMart (Catalog No ...
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bioRxiv - Biochemistry 2023Quote: ... using a His-tag Labeling Kit (NanoTemper Technologies, cat. no. MO-L018). For measurements using CBP30 ...
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Receptor tyrosine kinases regulate signal transduction through a liquid–liquid phase separated statebioRxiv - Cell Biology 2019Quote: The binding affinities were measured using the Monolith NT.115 (NanoTemper Technologies, GmbH). Proteins were fluorescently labelled with Atto488 according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2021Quote: ... Microscale thermophoresis results were analyzed by the MO affinity analysis software (NanoTemper Technologies GmbH)
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bioRxiv - Biochemistry 2022Quote: ... Data analysis was performed via MO Affinity Analysis Software v2.1.3 (Nanotemper Technologies, Munich, Germany).
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bioRxiv - Biochemistry 2022Quote: ... Binding curves were fitted and affinities (Kd) were calculated using MO.Affinity Analysis software (NanoTemper).
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Alpha-1-antitrypsin binds to the glucocorticoid receptor with biological significance in macrophagesbioRxiv - Immunology 2022Quote: ... Data were analyzed using the MO Affinity Analysis software suite (Nanotemper Technologies, Munich, Germany).
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bioRxiv - Cell Biology 2022Quote: ... employing the Monolith His-Tag Labeling Kit (RED-tris-NTA 2nd Generation kit, NanoTemper). The labeled protein was dissolved in a buffer containing PBS ...
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bioRxiv - Biochemistry 2024Quote: ... was labeled using the Monolith His-Tag Labeling Kit Red-tris-NTA (NanoTemper Technologies) and diluted in MST buffer (NanoTemper Technologies ...
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bioRxiv - Molecular Biology 2020Quote: ... Affinity measurements were conducted in Premium Capillaries Monolith NT.115 (MO-K025, NanoTemper Technologies GmbH) and repeated three times ...
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bioRxiv - Biochemistry 2022Quote: Binding affinity analysis by microscale thermophoresis was performed using the Monolith NT instrument (Nanotemper Technologies). All reactions (in triplicates ...
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bioRxiv - Cancer Biology 2023Quote: Binding affinities were estimated by microscale thermophoresis using a Monolith NT.115 device (NanoTemper, Germany). We measured the thermophoretic movements of labeled molecules within a temperature gradient ...
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bioRxiv - Molecular Biology 2024Quote: ... The Kd values and Hill coefficients (nH) were calculated using MO Affinity Analysis software (NanoTemper). All data were statistically analyzed and graphed using GraphPad Prism software (v5.0).
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bioRxiv - Cell Biology 2020Quote: ... Proteins were labeled using the RED-tris-NTA His tag protein labeling kit (Nanotemper Technologies). Labelled protein was added to a serial dilution of unlabeled DAG ...
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bioRxiv - Cell Biology 2022Quote: ... Monolith His-Tag Labeling Kit RED-tris-NTA 2nd Generation (NanoTemper Technologies, catalog #: MO-L018) was used to label recombinant His-α1-ERD (MyBioSource ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were labeled using the RED-tris-NTA His tag protein-labeling kit (Nanotemper Technologies). Labeled proteins were added to a serial dilution of unlabeled DAG or liposomes prepared in binding buffer (20 mM Tris ...
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bioRxiv - Cell Biology 2023Quote: His6-CTxA1 was labeled using Monolith NT His-Tag Labeling Kit RED-tris-NTA (NanoTemper). CTxB labeled with Alexa Fluor 647 (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... the binding affinity between SpaKC and SpaAleader was measured using Monolith NT.115 Pico (Nanotemper Technologies). SpaKC was fluorescently labelled by the labeling kit (Monolith Protein Labeling Kit RED-NHS 2nd Generation (Amine Reactive) ...
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bioRxiv - Molecular Biology 2023Quote: Binding affinity comparisons via microscale thermophoresis were performed using the Monolith NT.115 instrument (NanoTemper Technologies). The CTD polypeptides (CTD ...
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bioRxiv - Biochemistry 2023Quote: ... binding affinities were determined via microscale thermophoresis on a Monolith NT.115 instrument (NanoTemper Technologies, Germany). Briefly ...
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bioRxiv - Biochemistry 2023Quote: ... a 16-point affinity measurement was performed in duplicates using the Dianthus (NanoTemper Technologies GmbH, Germany) instrument ...
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bioRxiv - Molecular Biology 2024Quote: Binding affinity comparisons via microscale thermophoresis were performed using the Monolith NT.115 instrument (NanoTemper Technologies). The CTD polypeptides (CTD ...
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bioRxiv - Biochemistry 2022Quote: ... The binding affinity measurements were carried out using the modified manufacturer’s protocol (Monolith NT.115pico, NanoTemper Technologies) in the Center for Macromolecular Interactions in Harvard Medical School (Boston ...
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bioRxiv - Neuroscience 2023Quote: The affinity of the NbSyt1 was measured by microscale thermophoresis using the device NT.115Pico Monolith (NanoTemper). NbSyt1 was labelled with Alexa647 (as described above ...
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bioRxiv - Biochemistry 2022Quote: The affinity of SLC19A1 for TPP was determined using a NanoTemper Monolith NT.115 instrument (NanoTemper Technologies). The full-length SLC19A1 protein was fluorescently labelled with a Red-NHS 2nd Generation kit (NanoTemper Technologies ...
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bioRxiv - Pathology 2021Quote: Spike RBD proteins were labeled using Monolith His-Tag Labeling Kit RED-tris-NTA (NanoTemper Technologies) following manufacturer protocol at 2:1 protein to dye ratio ...
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bioRxiv - Biochemistry 2019Quote: ... was labeled with Monolith His-Tag Labeling Kit RED-tris-NTA kit (NanoTemper Technologies, München, Germany). Briefly ...
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bioRxiv - Physiology 2024Quote: ... cMyBP-C constructs were fluorescently labeled via His-tag using a commercially available kit (NanoTemper Technologies). Unlabeled sS1 or 15-hep myosin was titrated against 84 nM fluorescently labeled C3 or C0C7 protein ...
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bioRxiv - Biochemistry 2024Quote: ... was labeled using the Monolith His-Tag Labeling Kit RED-Tris-NTA second generation (NanoTemper Technologies), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: 300 nM 6His-tagged EF-Tu was incubated with 75 nM Red Tris NTA dye Generation 2 (NanoTemper Technologies) in binding buffer (287 mM NaCl ...
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bioRxiv - Molecular Biology 2021Quote: ... KD was determined according to the law of mass action using the program MO Affinity Analysis (NanoTemper Technologies) and results were plotted using Prism (Graphpad).
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bioRxiv - Biochemistry 2021Quote: ... Thermophoresis data were analyzed when fluorescence signals lies within the 10% acceptance range using MO Affinity Analysis software V2.1.2 (Nanotemper). Data were plotted using GraphPad Prism 4.0 software and fitted with nonlinear equation.
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bioRxiv - Microbiology 2020Quote: ... as recommended by the Monolith His-Tag Labeling Kit RED-tris-NTA protocol (NanoTemper Technologies, MO-L008). The binding buffer used for reactions [50 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Immunology 2023Quote: ... were fluorescently labeled using His-Tag Labeling Kit RED-Tris-NTA 2nd Generation (NanoTemper, Cat# MO-L018). The proteins (at fixed concentration ...
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bioRxiv - Biophysics 2021Quote: Thermal stability and binding affinity were determined using nanoscale differential scanning fluorimetry on a Prometheus NT.48 (NanoTemper Technologies). Capillaries were filled with 10 μL of respective samples in duplicates and loaded into the reading chamber ...
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bioRxiv - Cancer Biology 2020Quote: ... Data analyses and the dissociation constants (Kd) calculation were performed using MO Affinity Analysis software (NanoTemper Technologies, Munich, Germany). Binding of radiolabeled GTP was measured by means of the SPA ...
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bioRxiv - Biochemistry 2019Quote: ... C0-C7 was fluorescently labeled with a commercial His-tag labeling kit according to manufacturer instructions (Nanotemper technologies). His-labeled C0-C7 fluorescence was measured by a red LED at 60-90% excitation power (RED filter ...
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bioRxiv - Microbiology 2021Quote: ... TgPRP4K protein was labeled using the Monolith His-Tag Labeling Kit RED-tris-NTA 2nd Generation (NanoTemper Technologies). The labeled TgPRP4K protein was adjusted to 100 nM with a buffer containing 30 mM Hepes (PH 7.5) ...
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bioRxiv - Molecular Biology 2020Quote: ... We measured the fluorescence signal from Gαi1β1γ1 by using the Monolith His-Tag Labeling Kit RED-tris-NTA 2nd Generation kit (NanoTemper Technologies). The samples were prepared in a buffer containing 20 mM sodium phosphate (pH 6.9) ...
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bioRxiv - Biochemistry 2019Quote: ... was labelled with 100 μM Monolith NTTM His-Tag Labeling kit RED-tris-NTA (L008, NANOTEMPER, Munich, Germany) 30 min at room temperature (RT ...
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bioRxiv - Plant Biology 2022Quote: ... The N-terminal His-tag was labelled with the RED-tris-NTA kit (NanoTemper Technologies GmbH, Munich, Germany). After 10 minutes incubation on ice ...
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bioRxiv - Microbiology 2023Quote: ... + 0.5% DDM and was labeled using a His-Tag Labeling Kit RED-tris-NTA 2nd Generation Kit (NanoTemper) via the manufacturers protocol in 1.7 ml low-adhesion tubes ...
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bioRxiv - Cell Biology 2024Quote: Purified protein was labeled using the His-Tag labeling Kit RED-tris-NTA 2nd Generation (Nanotemper, MO-L018) following the manufacturer’s directions ...
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bioRxiv - Biochemistry 2020Quote: The binding affinities of compound 20 to Sc-Dyn-lysoMut was measured using Monolith NT.115 (NanoTemper Technologies, Munich, Germany). Sc-Dyn-lysoMut was labeled with the Monolith NT Protein Labeling Kit RED-NHS ...
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bioRxiv - Biophysics 2020Quote: The binding affinity of spike to Sb23 was measured by microscale thermophoresis using the MST NT.115 device (NanoTemper Technologies). Sb23 was labelled via the His6-tag with RED-tris-NTA dye (NanoTemper Technologies ...
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bioRxiv - Biochemistry 2019Quote: Apparent Kd values were computed fitting the compound concentration-dependent changes in normalized fluorescence (Fnorm) by the MO Affinity Analysis software provided by Nanotemper. Final results were obtained averaging four independent experiments.
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bioRxiv - Molecular Biology 2023Quote: The affinity between RNA and protein and between proteins was measured by MST on a Monolith NT.115 (NanoTemper, Germany). Replicates represent individual measurements performed on different days using proteins from the same prep ...
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bioRxiv - Microbiology 2022Quote: ... Normalization of the fluorescence signal and fitting to the Hill equation were performed using the software MO Affinity Analysis v2.3 (NanoTemper Technologies). For each sample ...
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bioRxiv - Biophysics 2021Quote: 6xHis-RAD51 was labelled with the Monolith His-Tag Labeling Kit RED-tris-NTA 2nd Generation kit (NanoTemper Technologies), which specifically recognizes the hexahistidine tail of the recombinant protein.
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bioRxiv - Biophysics 2021Quote: The data was analyzed and the Ktet (Eq. 1) or EC50 (Eq. 2) calculated using the MO affinity analysis software (NanoTemper, Germany), considering a 1:1 model.