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201 - 204 of 204 citations for Recombinant Cyan Fluorescent Protein His tagged since 2019

• Listeria phages induce Cas9 degradation to protect lysogenic genomes

  Beatriz A. Osuna, et al., bioRxiv - Microbiology 2019

  Quote: The binding affinities of anti-CRISPR proteins to SpyCas9 were calculated using microscale thermophoresis (MST) on the Monolith NT.115 instrument (NanoTemper Technologies GmbH, Munich, Germany). For AcrIIA1/AcrIIA2b.3 with WT or mutant Cas9-gRNA complexes ...
• The K⁺ exchange antiporter 3 senses the chloroplast energy status to synchronize photosynthesis

  Michal Uflewski, et al., bioRxiv - Plant Biology 2022

  Quote: ... 50 nM of protein sample in PBS together with indicated concentrations of nucleotides were loaded into standard Monolith NT.115 capillaries (NanoTemper Technologies GmbH, Munich, Germany). MST measurement was performed using the Monolith NT.115 instrument (NanoTemper Technologies GmbH ...
• The Strongest Protein Binder is Surprisingly Labile

  Alba Fernandez-Calvo, et al., bioRxiv - Biophysics 2024

  Quote: The (I91)2-ClfAN2N3-(I91)2 polyprotein was fluorescently labeled by utilizing the commercial protein Labeling Kit RED-NHS 2nd Generation (Nanotemper Technologies GmbH, Munich, Germany). The dye carries a reactive NHS-ester group that reacts with primary amines in the protein to form a covalent bond ...
• Molecular basis of UHRF1 allosteric activation for synergistic histone modification binding by PI5P

  Papita Mandal, et al., bioRxiv - Biochemistry 2021

  Quote: Fluorophore-labeled protein was incubated with various ligands at room temperature for 15 minutes before measuring on Monolith NT.115 (NanoTemper, 80% LED power, 40% MST power). For competition assays ...