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Citations for NanoTemper :
151 - 169 of 169 citations for Rat Translational Activator Of Cytochrome C Oxidase 1 TACO1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... for 30 min with 50 nM RED-tris-NTA 2nd Generation dye from the Monolith His-Tag Labeling Kit (Cat.#MO-L018; NanoTemper Technologies). The labeling reaction mixture was centrifuged for 10 min at 4°C and 15,000 x g to remove protein aggregates ...
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bioRxiv - Microbiology 2021Quote: ... Phr2 and Pga4 proteins and Bull Serum Albumin (BSA) were labeled using the Monolith™ RED-NHS Protein Labeling Kit (NanoTemper Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: 50 µg synthesized sP26 (Davids Biotechnologie, Regensburg, Germany) was fluorescently labeled with the Monolith NT Protein Labeling Kit RED (NanoTemper, Munich, Germany) according to manufacturer’s protocol ...
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Long-Range Electrostatic Interactions Significantly Modulate the Affinity of Dynein for MicrotubulesbioRxiv - Biophysics 2021Quote: ... We labeled the MTBDs with a 2nd Generation RED-tris-NTA His-Tag Labeling Kit (MO-L018, NanoTemper Technologies, GmbH, Munich, Germany) per the manufacturer’s instructions with slight modifications (43) ...
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bioRxiv - Cancer Biology 2019Quote: BRCA1 tBRCT wild type or mutant domains used in the assay were labeled with NT-647-NHS fluorescent dye using the Monolith NT™ Protein Labeling Kit (NanoTemper Technologies). Assays were carried out in 20mM Tris buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 µM His6-SUMO-AGO1-NcGN was orthogonally labeled on Cystein-192 using the Monolith NT™ Protein Labeling Kit Red – Maleimide (NanoTemper Technologies). The labeling was performed according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... PD-1 and its mutant were fluorescently labelled according the standard labeling protocol of the NanoTemper Protein Labeling Kit RED-NHS (L001-NanoTemper Technologies, Munich, Germany). In MST experiments ...
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bioRxiv - Neuroscience 2021Quote: ... Recombinant human FTO protein was labeled with His-tag using Monolith His-Tag Labeling Kit RED-tris-NTA (NanoTemper Technologies GmbH; MO-L008). The labelled FTO protein (target ...
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bioRxiv - Biophysics 2020Quote: ... 1 mM CaCl2 and 0.05 % (v/v) Tween-20 using a Nanotemper Monolith NT.115 (Nanotemper). To measure the affinity of zGFRα1D1-3-zGDNFmat ...
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bioRxiv - Biophysics 2024Quote: The (I91)2-ClfAN2N3-(I91)2 polyprotein was fluorescently labeled by utilizing the commercial protein Labeling Kit RED-NHS 2nd Generation (Nanotemper Technologies GmbH, Munich, Germany). The dye carries a reactive NHS-ester group that reacts with primary amines in the protein to form a covalent bond ...
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bioRxiv - Neuroscience 2023Quote: ... Fluorescent NbSyt1 was incubated with different dilutions of purified Syt-1(97-421) using Premium Coated Capillaries (NanoTemper). For operation of the instrument and evaluation of affinity data ...
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bioRxiv - Biochemistry 2020Quote: ... histidine tagged HMGB1 was labeled by the his-tag specific NT-647 dye (Monolith NTTM Protein Labelling Kit RED-NHS, NanoTemper® Technologies GmbH, Mu□nchen, Germany), for 30 minutes at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... 25 μl of a 1 mg/ml HiSiaP protein solution was prepared and 10 μl was soaked into a glass capillary (standard capillary, NanoTemper). The start and end temperature were set to 20 °C and 90 °C ...
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bioRxiv - Biophysics 2021Quote: The data was analyzed and the Ktet (Eq. 1) or EC50 (Eq. 2) calculated using the MO affinity analysis software (NanoTemper, Germany), considering a 1:1 model.
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bioRxiv - Microbiology 2019Quote: ... The binding affinities were determined to fit the data points to a quadratic equation (equation 1) using non-linear regression using MO Affinity software (NanoTemper Technologies), Prism (GraphPad Inc ...
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bioRxiv - Biophysics 2020Quote: The thermal stability of target proteins in different buffers and the potential stabilizing effects of ligands was evaluated at 1 μM using a direct thermal shift assay using a Tycho NT6 instrument (Nanotemper Technologies), monitoring intrinsic protein fluorescence at 330 and 350 nm during a thermal ramp from 35 °C to 95 °C ...
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bioRxiv - Cell Biology 2019Quote: ... 10 ⌈L of 1 mg/mL ADCs were loaded into standard capillaries and mounted in a Prometheus NT.48 (Nanotemper Technologies, Germany) with excitation near UV ...
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bioRxiv - Cell Biology 2023Quote: ... The interaction of NRP-1 structural variants with FOL-peptides was measured using a Monolith NT.115 [NT.115Pico/NT.LabelFree] instrument (NanoTemper Technologies, Munich, Germany). For more details see supplementary materials and methods.
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bioRxiv - Neuroscience 2023Quote: ... into 50 nM of fluorescently labeled Munc13-1 constructs in a premium-coated glass capillary using a Monolith NT.115 (Nanotemper Technologies, San Francisco, CA).