Labshake search
Citations for NanoTemper :
101 - 150 of 242 citations for Rat Insulin Like Growth Factor Binding Protein 4 IGFBP4 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: The amine residues of purified KsgA were fluorescently labeled with NHS red using the Protein Labeling Kit RED-NHS 2nd Generation (Cat # MO-L011 Nanotemper). The labeling reaction was performed according to the manufacturer’s protocol by mixing KsgA at a final concentration of 20 μM with a 3-fold molar excess of dye at room temperature for 30 minutes in the dark ...
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bioRxiv - Molecular Biology 2022Quote: ... The TPR-CHAT and its mutants were fluorescence-labeled using the Protein Labeling Kit RED-NHS 2nd Generation (NanoTemper Technologies) at 10 μM ...
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bioRxiv - Developmental Biology 2023Quote: ... His-tagged RALF proteins were labeled with red dyes with the His-Tag labelling kit RED-tris-NTA 2nd Generation (NanoTemper) according to the manufacturers’ recommendation ...
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The activator domain of bacterial collagenases drives collagen recognition, unwinding and processingbioRxiv - Biochemistry 2023Quote: ColG-variants were labeled using the Monolith NT™ Protein Labeling Kit RED-NHS 2nd Generation Amine reactive (NanoTemper, Germany) and the yield of labelled protein and the degree of labelling were determined according to the manufacturer’s manual ...
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bioRxiv - Neuroscience 2024Quote: We followed the manufacturer’s instructions to label APOE3 and APOE4 using the Monolith protein labeling kit RED-NHS 2nd generation (NanoTemper Technologies). In summary ...
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bioRxiv - Molecular Biology 2023Quote: MST experiments to measure the binding affinities between TadAs and the 19-nt DNA substrate were performed on a Monolith NT.115 system (NanoTemper Technologies, Germany) using the nano BLUE detector ...
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bioRxiv - Immunology 2022Quote: ... Germany) and MST-Red-MIF was prepared using the Monolith Protein Labeling Kit RED-NHS 2nd Generation from NanoTemper (Munich, Germany), following the manufacturers’ instructions ...
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bioRxiv - Biophysics 2022Quote: His-tagged NCTD was fluorescently labelled with NT-647 amine reactive dye from the Monolith NT Protein Labelling kit RED-NHS (NanoTemper Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... RBD was labeled with the RED-NHS 2nd generation Monolith Protein Labeling Kit according to the manufacturer’s instructions (Nanotemper; MO-L011). The fluorescent group was conjugated to RBD through primary amines ...
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bioRxiv - Biochemistry 2021Quote: 6xHis-eIF1 was labeled with a Cy5 fluorophore through non-covalent linkage to the His-tag moiety using the Monolith Protein Labeling Kit RED-tris-NTA 2nd generation (NanoTemper Technologies). 40S subunits (0.58 nM –1.2 μM ...
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bioRxiv - Microbiology 2022Quote: ... were labeled with the Monolith Protein Labeling Kit RED-NHS 2nd generation according to the recommendations of the manufacturer (NanoTemper Technologies). Briefly ...
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bioRxiv - Microbiology 2022Quote: Purified WT or mutant NSP12 recombinant proteins were labeled with Cytidine-5 dye using the Monolith His-Tag labeling kit (NanoTemper Technologies), and then labeled NSP12 proteins were diluted to 700 nM in PBST buffer ...
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bioRxiv - Biochemistry 2023Quote: ... His LRRK2 Armadillo (1–552) K439E and E240R were labeled using RED-NHS 2nd Generation (Amine Reactive) Protein Labeling Kit (NanoTemper Technologies). For all experiments ...
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bioRxiv - Biophysics 2023Quote: The recombinant RAD52 FL protein was labeled with the Monolith labeling kit RED-NHS (ammine dye NT-647-NHS) according to manufacturer indications (NanoTemper Technologies). Before MST experiments ...
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The activator domain of bacterial collagenases drives collagen recognition, unwinding and processingbioRxiv - Biochemistry 2023Quote: ... ColG variants were labeled using the Monolith NT™ Protein Labeling Kit RED-NHS 2nd Generation Amine reactive (NanoTemper Technologies, Germany). After labeling ...
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bioRxiv - Plant Biology 2024Quote: ... Purified insect cell-expressed RACK1A was fluorescently labeled with Protein Labeling Kit RED-NHS 2nd Generation (NanoTemper Technologies GmbH MO-L011) via amine conjugation ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Purified TRIM21 PRYSPRY domain was labeled using Protein Labeling Kit RED-NHS 2nd Generation (MO-L011, NanoTemper Technologies GmbH, Munich, Germany). Labeled protein was diluted to 50 nM in PBST (137 mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: 50 µg synthesized sP26 (Davids Biotechnologie, Regensburg, Germany) was fluorescently labeled with the Monolith NT Protein Labeling Kit RED (NanoTemper, Munich, Germany) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2019Quote: BRCA1 tBRCT wild type or mutant domains used in the assay were labeled with NT-647-NHS fluorescent dye using the Monolith NT™ Protein Labeling Kit (NanoTemper Technologies). Assays were carried out in 20mM Tris buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 µM His6-SUMO-AGO1-NcGN was orthogonally labeled on Cystein-192 using the Monolith NT™ Protein Labeling Kit Red – Maleimide (NanoTemper Technologies). The labeling was performed according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: Protein-protein interactions were monitored by MST using a Monolith NT.115 instrument (Nanotemper Technologies). His6-MyoVa GTD was labeled using RED-NHS 2nd Generation (Amine Reactive ...
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Long-Range Electrostatic Interactions Significantly Modulate the Affinity of Dynein for MicrotubulesbioRxiv - Biophysics 2021Quote: We measured the binding affinity of MTBDs (fluorescently labeled target) to microtubules (unlabeled ligand) using a Monolith NT.115 MST system (NanoTemper Technologies, GmbH, Munich, Germany). We labeled the MTBDs with a 2nd Generation RED-tris-NTA His-Tag Labeling Kit (MO-L018 ...
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bioRxiv - Biophysics 2020Quote: ... PD-1 and its mutant were fluorescently labelled according the standard labeling protocol of the NanoTemper Protein Labeling Kit RED-NHS (L001-NanoTemper Technologies, Munich, Germany). In MST experiments ...
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bioRxiv - Neuroscience 2021Quote: ... Recombinant human FTO protein was labeled with His-tag using Monolith His-Tag Labeling Kit RED-tris-NTA (NanoTemper Technologies GmbH; MO-L008). The labelled FTO protein (target ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Recombinant METTL16 MTD (residues 1-291) protein was labelled via His-tag using Monolith His-Tag Labeling Kit RED-tris-NTA 2nd Generation (NanoTemper Technologies GmbH, Germany). The labelled protein was diluted in MST buffer (10 mM Na-phosphate buffer ...
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bioRxiv - Biochemistry 2022Quote: Protein–protein interactions were monitored by microscale thermophoresis using a Monolith NT.115 instrument (Nanotemper Technologies). His LRRK2 Armadillo (1-552) ...
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bioRxiv - Biochemistry 2023Quote: Protein–protein interactions were monitored by microscale thermophoresis using a Monolith NT.115 instrument (NanoTemper Technologies). His LRRK2 Armadillo (1–552 ...
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bioRxiv - Biophysics 2024Quote: The (I91)2-ClfAN2N3-(I91)2 polyprotein was fluorescently labeled by utilizing the commercial protein Labeling Kit RED-NHS 2nd Generation (Nanotemper Technologies GmbH, Munich, Germany). The dye carries a reactive NHS-ester group that reacts with primary amines in the protein to form a covalent bond ...
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bioRxiv - Microbiology 2022Quote: ... Protein-protein interactions were analyzed at 20°C by MST on a Monolith NT.115 (NanoTemper technologies) using standard capillaries ...
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bioRxiv - Biochemistry 2020Quote: ... histidine tagged HMGB1 was labeled by the his-tag specific NT-647 dye (Monolith NTTM Protein Labelling Kit RED-NHS, NanoTemper® Technologies GmbH, Mu□nchen, Germany), for 30 minutes at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: The affinity between RNA and protein and between proteins was measured by MST on a Monolith NT.115 (NanoTemper, Germany). Replicates represent individual measurements performed on different days using proteins from the same prep ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein interaction was measured in standard capillaries (NanoTemper), 100% excitation power and medium or high MST power (IR-laser intensity) ...
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bioRxiv - Immunology 2022Quote: Protein-protein interactions were analyzed via microscale thermophoresis on a Monolith NT.115 instrument equipped with green/red filters (NanoTemper Technologies, Munich, Germany). Measurements were performed at 25°C at both 40% and 80% MST power ...
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bioRxiv - Biochemistry 2020Quote: ... Thermally-driven protein unfolding was monitored in Tycho quartz capillaries (Nanotemper) using a Tycho NT.6 instrument (Nanotemper) ...
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bioRxiv - Microbiology 2022Quote: ... Protein quality was verified using a Tycho NT.6 system (NanoTemper) and ACE2 activity assay kit (SensoLyte ...
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bioRxiv - Microbiology 2019Quote: ... Hexahistidine-tagged ALIX protein was labeled by NTA-dye (NanoTemper Technologies) using manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Protein samples (0.1 mg/mL) were loaded into glass capillaries (NanoTemper) and then subjected to a temperature gradient from 20 to 95 °C at a heating rate of 1 °C per min ...
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bioRxiv - Immunology 2023Quote: ... Protein samples (0.1 mg/mL) were loaded into glass capillaries (NanoTemper) and then subjected to a temperature gradient from 20 to 95°C at a heating rate of 1°C per min ...
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bioRxiv - Biophysics 2024Quote: ... CRBN protein was labelled with RED-Maleimide 2nd Generation dye (NanoTemper) optimized for degree-of-labeling of 1:1 molar ratio calculated by nanodrop ...
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A pectin-binding peptide with a structural and signaling role in the assembly of the plant cell wallbioRxiv - Plant Biology 2023Quote: ... protein quality was assessed using a Tycho NT.6 (NanoTemper, Munich, Germany). Labeled protein was dissolved in MST buffer (50mM Tris-HCl pH 7.8 ...
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bioRxiv - Microbiology 2023Quote: Protein thermostability was evaluated with a Prometheus NT.48 instrument (NanoTemper Technologies). Proteins at a concentration of 1 mg/mL were loaded to the grade capillaries and the intrinsic fluorescence signal was measured while temperature was increased by 1°C/min ...
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bioRxiv - Biochemistry 2023Quote: ... Ten microliters of proteins were loaded into a capillary (PR-C002, Nanotemper) and thermal denaturation was recorded using a 1 °C/min ramp from 20 to 95 °C ...
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bioRxiv - Biochemistry 2022Quote: Protein thermal stability was measured using nanoDSF on a Prometheus panta instrument (NanoTemper) (39) ...
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bioRxiv - Biochemistry 2020Quote: ... 10μL of the diluted protein was loaded into NanoDSF Grade Standard Capillaries (NanoTemper) and melting temperatures were determined using a Prometheus NT4.8 NanoDSF instrument according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2020Quote: We determined protein melting temperatures using the Prometheus NT.48 instrument (NanoTemper Technologies). Purified FabI was diluted to a concentration of 25 μM in 50 nM HEPES ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein series was loaded into Monolith NT.115 Premium Capillaries (NanoTemper Technologies) and thermophoresis was measured using MO.Control v1.6.1 software ...
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bioRxiv - Biophysics 2022Quote: Recombinantly purified protein constructs were labelled with RED-tris NTA dye (NanoTemper Technologies GmbH) in PBS to a concentration of 100 nM ...
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bioRxiv - Pathology 2021Quote: ... Protein quality was determined using a Tycho NT.6 (Figures S4 & S5) (NanoTemper Technologies). Human hnRNPC was purchased from Abnova (catalog# H00003183P01S.)
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bioRxiv - Plant Biology 2023Quote: ... mixed 1:1 with the labelled protein and loaded into standard capillaries (NanoTemper Technologies). MST measurements were recorded using 20 % LED power and 20 to 80 % MST power and analysed using MO ...
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bioRxiv - Biophysics 2024Quote: ... The melting temperature for characterization of protein thermal stability were done using NanoDSF (NanoTemper) with protein concentration at 0.2mg/ml ...