Labshake search
Citations for NanoTemper :
101 - 150 of 231 citations for QuantiFluo Protein Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2023Quote: Thermal shift assay was performed using Tycho NT.6 (NanoTemper Technologies). Envelope ectodomain SOSIPs were diluted (0.15LmgLml−1 ...
-
bioRxiv - Molecular Biology 2023Quote: The affinity between RNA and protein and between proteins was measured by MST on a Monolith NT.115 (NanoTemper, Germany). Replicates represent individual measurements performed on different days using proteins from the same prep ...
-
bioRxiv - Neuroscience 2023Quote: ... Thermal shift assay was performed on Prometheus Panta NT.48 from NanoTemper technologies by measuring the intrinsic dual-UV fluorescence change in tryptophan and tyrosine residues in proteins at emission wavelengths of k = 330 and 350 nm.The ratio of the recorded emission intensities (Em350nm/Em330nm) ...
-
bioRxiv - Biochemistry 2023Quote: ... microscale thermophoresis (MST) assays were performed on a Monolith X (Nanotemper Technologies) using standard capillaries (MO-K022 ...
-
bioRxiv - Biochemistry 2024Quote: Spectral shift assay was performed on a Monolith X instrument (NanoTemper Technologies) in parallel with MST measurements ...
-
bioRxiv - Molecular Biology 2023Quote: ... The protein interaction was measured in standard capillaries (NanoTemper), 100% excitation power and medium or high MST power (IR-laser intensity) ...
-
bioRxiv - Immunology 2022Quote: Protein-protein interactions were analyzed via microscale thermophoresis on a Monolith NT.115 instrument equipped with green/red filters (NanoTemper Technologies, Munich, Germany). Measurements were performed at 25°C at both 40% and 80% MST power ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: The MST assay was carried out on a Monolith NT.115 (NanoTemper Technologies). CK1γ2 was prepared at concentrations from 0.305 to 10000□nM and fluorescence dye from the Monolith His-tag Labeling kit RED-tris-NTA (NanoTemper Technologies ...
-
bioRxiv - Biochemistry 2020Quote: ... Thermally-driven protein unfolding was monitored in Tycho quartz capillaries (Nanotemper) using a Tycho NT.6 instrument (Nanotemper) ...
-
bioRxiv - Microbiology 2022Quote: ... Protein quality was verified using a Tycho NT.6 system (NanoTemper) and ACE2 activity assay kit (SensoLyte ...
-
bioRxiv - Microbiology 2019Quote: ... Hexahistidine-tagged ALIX protein was labeled by NTA-dye (NanoTemper Technologies) using manufacturer’s protocol ...
-
bioRxiv - Immunology 2022Quote: ... Protein samples (0.1 mg/mL) were loaded into glass capillaries (NanoTemper) and then subjected to a temperature gradient from 20 to 95 °C at a heating rate of 1 °C per min ...
-
bioRxiv - Immunology 2023Quote: ... Protein samples (0.1 mg/mL) were loaded into glass capillaries (NanoTemper) and then subjected to a temperature gradient from 20 to 95°C at a heating rate of 1°C per min ...
-
bioRxiv - Biophysics 2024Quote: ... CRBN protein was labelled with RED-Maleimide 2nd Generation dye (NanoTemper) optimized for degree-of-labeling of 1:1 molar ratio calculated by nanodrop ...
-
bioRxiv - Biochemistry 2020Quote: The microscale thermophoresis (MST) assay was performed using a Monolith NT.115 instrument (NanoTemper Technologies). For the MST assay ...
-
bioRxiv - Biophysics 2022Quote: EspB1-332 and free phospholipid or model membrane interaction assay was done using MST (Nanotemper)(43) ...
-
bioRxiv - Immunology 2023Quote: ... The final sample was validated using SDS-PAGE and thermostability assay (Nanotemper NT.48 nanoDSF).
-
bioRxiv - Developmental Biology 2023Quote: ... microscale thermophoresis (MST) assays were carried out by using a Monolish NT.115 (NanoTemper Technologies). His-tagged RALF proteins were labeled with red dyes with the His-Tag labelling kit RED-tris-NTA 2nd Generation (NanoTemper ...
-
bioRxiv - Biochemistry 2023Quote: Thermal stability assays were performed using a Prometheus NT.48 nanoDSF instrument (NanoTemper Inc., Germany). 50 μl of sample per condition were prepared to perform experiments in triplicates using standard-treated glass capillaries ...
-
bioRxiv - Molecular Biology 2024Quote: MicroScale Thermophoresis (MST) assay was performed on a Monolith NT.115 instrument (NanoTemper Technologies GmbH) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: Protein thermostability was evaluated with a Prometheus NT.48 instrument (NanoTemper Technologies). Proteins at a concentration of 1 mg/mL were loaded to the grade capillaries and the intrinsic fluorescence signal was measured while temperature was increased by 1°C/min ...
-
A pectin-binding peptide with a structural and signaling role in the assembly of the plant cell wallbioRxiv - Plant Biology 2023Quote: ... protein quality was assessed using a Tycho NT.6 (NanoTemper, Munich, Germany). Labeled protein was dissolved in MST buffer (50mM Tris-HCl pH 7.8 ...
-
bioRxiv - Biochemistry 2023Quote: ... Ten microliters of proteins were loaded into a capillary (PR-C002, Nanotemper) and thermal denaturation was recorded using a 1 °C/min ramp from 20 to 95 °C ...
-
bioRxiv - Plant Biology 2022Quote: ... cAMP binding assays to G-actin were performed with the Monolith NT.115 (Nanotemper, Munich, Germany) using 50 nM NTred-maleimid 2nd generation labelled G-actin and increasing concentrations of cAMP ...
-
bioRxiv - Cancer Biology 2023Quote: Label-free thermal shift assay experiments were performed with a Tycho NT.6 instrument (NanoTemper Technologies) based on a reported protocol 76 ...
-
bioRxiv - Biochemistry 2022Quote: Protein thermal stability was measured using nanoDSF on a Prometheus panta instrument (NanoTemper) (39) ...
-
bioRxiv - Biochemistry 2020Quote: ... 10μL of the diluted protein was loaded into NanoDSF Grade Standard Capillaries (NanoTemper) and melting temperatures were determined using a Prometheus NT4.8 NanoDSF instrument according to the manufacturer’s instruction ...
-
bioRxiv - Microbiology 2020Quote: We determined protein melting temperatures using the Prometheus NT.48 instrument (NanoTemper Technologies). Purified FabI was diluted to a concentration of 25 μM in 50 nM HEPES ...
-
bioRxiv - Molecular Biology 2023Quote: ... The protein series was loaded into Monolith NT.115 Premium Capillaries (NanoTemper Technologies) and thermophoresis was measured using MO.Control v1.6.1 software ...
-
bioRxiv - Microbiology 2023Quote: ... thermal shift assays were performed with nanoDSF integrated in the Prometheus NT.48 (NanoTemper Technologies GmbH, Germany). Protein solutions of 75-100 µM of YopR WT ...
-
bioRxiv - Biochemistry 2024Quote: Thermal shift assays were carried out in triplicate on the Prometheus NT.48 nanoDSF instrument (NanoTemper Technologies) using the manufacturer-designated capillaries ...
-
bioRxiv - Biochemistry 2021Quote: MST assays were performed in a Monolith NT.115 using Standard Treated Glass Capillaries (NanoTemper Technologies MO-K002). His-TEV-UCH37(C88S)-RPN13C was labelled with Monolith His-Tag labeling Kit RED-tris-NTA (MO-L008 ...
-
bioRxiv - Biophysics 2022Quote: Recombinantly purified protein constructs were labelled with RED-tris NTA dye (NanoTemper Technologies GmbH) in PBS to a concentration of 100 nM ...
-
bioRxiv - Pathology 2021Quote: ... Protein quality was determined using a Tycho NT.6 (Figures S4 & S5) (NanoTemper Technologies). Human hnRNPC was purchased from Abnova (catalog# H00003183P01S.)
-
bioRxiv - Plant Biology 2023Quote: ... mixed 1:1 with the labelled protein and loaded into standard capillaries (NanoTemper Technologies). MST measurements were recorded using 20 % LED power and 20 to 80 % MST power and analysed using MO ...
-
bioRxiv - Biophysics 2024Quote: ... The melting temperature for characterization of protein thermal stability were done using NanoDSF (NanoTemper) with protein concentration at 0.2mg/ml ...
-
bioRxiv - Cell Biology 2022Quote: ... employing the Monolith His-Tag Labeling Kit (RED-tris-NTA 2nd Generation kit, NanoTemper). The labeled protein was dissolved in a buffer containing PBS ...
-
bioRxiv - Microbiology 2023Quote: Nucleotide binding assays based on microscale thermophoresis were performed with a Monolith NT.115 instrument (NanoTemper Technologies GmbH, Germany) using Monolith NT Premium Capillaries ...
-
bioRxiv - Biochemistry 2022Quote: ... Protein folding and quality control was accomplished using nDSF on a Tycho NT.6 (NanoTemper) which monitors protein thermal unfolding using intrinsic fluorescence at 330 and 350 nm ...
-
bioRxiv - Neuroscience 2023Quote: Thermal denaturation of ApoE proteins was studied by using a NanoDSF Prometheus instrument (NanoTemper, Germany). The protein samples at a concentration of 0.2 mg/ml were heated at a scan rate of 1 °C/min from 25 to 95 °C ...
-
bioRxiv - Evolutionary Biology 2024Quote: We measured protein stability using nano-differential scanning fluorimetry on a Prometheus NT.48 (NanoTemper). After purification and his-tag cleavage ...
-
bioRxiv - Biochemistry 2024Quote: ... FabF protein was labeled with the RED-MALEIMIDE or RDE-NHS dye (Nanotemper Technologies, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2019Quote: ... was labeled with Monolith His-Tag Labeling Kit RED-tris-NTA kit (NanoTemper Technologies, München, Germany). Briefly ...
-
bioRxiv - Biochemistry 2020Quote: DSF assays were performed on LH3 wild-type and mutants using a Tycho NT.6 instrument (NanoTemper Technologies GmbH, Germany). LH3 samples at a concentration of 1 mg/mL in a buffer composed of 25 mM HEPES ...
-
bioRxiv - Biophysics 2019Quote: The stability of the different proteins was measured by nanoDSF (Prometheus NT.48, NanoTemper Technologies, GmbH). The proteins were first dialyzed against 1 l of gel filtration buffer supplemented with 5 mM EDTA overnight at 4 °C and subsequently 2x against 1 l of gel filtration buffer without EDTA overnight at 4 °C ...
-
bioRxiv - Microbiology 2020Quote: ... Each protein (10 μM) was labeled with NT-647-NHS reactive dye (30 μM) (Nanotemper, Germany), which reacted efficiently with the primary amines of the proteins to form a stable dye protein conjugate ...
-
bioRxiv - Molecular Biology 2022Quote: The thermal stability of the proteins was determined using Prometheus NT.48 nanoDSF instrument from NanoTemper Technologies ...
-
bioRxiv - Cell Biology 2021Quote: Binding of cevipabulin to the seventh site was detected with an MST assay with a Monolith NT.115 instrument (NanoTemper Technologies). Purified tubulin was labeled using the Monolith protein labeling kit RED-NHS (NanoTemper Technologies ...
-
bioRxiv - Biophysics 2020Quote: The thermal stability of target proteins in different buffers and the potential stabilizing effects of ligands was evaluated at 1 μM using a direct thermal shift assay using a Tycho NT6 instrument (Nanotemper Technologies), monitoring intrinsic protein fluorescence at 330 and 350 nm during a thermal ramp from 35 °C to 95 °C ...
-
bioRxiv - Biophysics 2020Quote: ... The assay was performed in duplicate and the values reported were generated through the usage of MO Affinity Analysis software (NanoTemper Technologies).