Labshake search
Citations for NanoTemper :
1 - 50 of 202 citations for Protein DNA Interaction Assay since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: ... The protein interaction was measured in standard capillaries (NanoTemper), 100% excitation power and medium or high MST power (IR-laser intensity) ...
-
bioRxiv - Cell Biology 2020Quote: Protein-protein interactions were monitored by MST using a Monolith NT.115 instrument (Nanotemper Technologies). His6-MyoVa GTD was labeled using RED-NHS 2nd Generation (Amine Reactive ...
-
bioRxiv - Biochemistry 2022Quote: Protein–protein interactions were monitored by microscale thermophoresis using a Monolith NT.115 instrument (Nanotemper Technologies). His LRRK2 Armadillo (1-552) ...
-
bioRxiv - Biochemistry 2023Quote: Protein–protein interactions were monitored by microscale thermophoresis using a Monolith NT.115 instrument (NanoTemper Technologies). His LRRK2 Armadillo (1–552 ...
-
bioRxiv - Microbiology 2022Quote: ... Protein-protein interactions were analyzed at 20°C by MST on a Monolith NT.115 (NanoTemper technologies) using standard capillaries ...
-
bioRxiv - Biophysics 2022Quote: EspB1-332 and free phospholipid or model membrane interaction assay was done using MST (Nanotemper)(43) ...
-
bioRxiv - Immunology 2022Quote: Protein-protein interactions were analyzed via microscale thermophoresis on a Monolith NT.115 instrument equipped with green/red filters (NanoTemper Technologies, Munich, Germany). Measurements were performed at 25°C at both 40% and 80% MST power ...
-
bioRxiv - Molecular Biology 2022Quote: ... we evaluated the interaction of recombinant PfPHB1 and PfPHB2 proteins using Monolith NT.115 instrument (NanoTemper Technologies, Munich, Germany). Thermophoretic movement of a fluorescently labelled protein is measured by monitoring the fluorescence distribution ...
-
bioRxiv - Cell Biology 2024Quote: MST measurements were carried out using premium-coated capillaries to reduce non-specific interaction of the proteins with the glass surface on a Monolith NT.115 red-blue (Nanotemper, Munich, Germany), as described previously (Zuidema et al. ...
-
bioRxiv - Pathology 2023Quote: ... the possible isoprothiolane-MoIcl1 interaction was assayed on Prometheus NT.48 (NanoTemper, Germany) as previously described.60 The more detailed information is provided in the Supporting Information.
-
bioRxiv - Molecular Biology 2022Quote: ... All interactions were measured in Monolith NT.115 Standard Treated Capillaries (MO-K002, Nanotemper) at 25℃ with an MST-on time of 20 seconds and an MST-off time of 5 seconds ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... The interaction was further validated with Microscale Thermophoresis (MST) methods using Monolith NT.115 (Nanotemper). Rg3 titrated in different concentrations to purified recombinant human GRB2 protein ...
-
bioRxiv - Cancer Biology 2019Quote: The Grb2 and Shp2 interactions were measured using the Monolith NT.115 MST instrument from Nanotemper Technologies ...
-
bioRxiv - Biochemistry 2021Quote: Protein thermal stability was measured in a label-free fluorescence assay using a Prometheus NT.48 (NanoTemper, Germany). Briefly ...
-
bioRxiv - Cancer Biology 2019Quote: BRCA1 tBRCT wild type or mutant domains used in the assay were labeled with NT-647-NHS fluorescent dye using the Monolith NT™ Protein Labeling Kit (NanoTemper Technologies). Assays were carried out in 20mM Tris buffer ...
-
bioRxiv - Biochemistry 2023Quote: ... Each protein dilution was mixed with one volume of labeled DNA and filled into Monolith NT standard treated capillaries (NanoTemper Technologies GmbH). Thermophoresis was measured using a Monolith NT.115 instrument (NanoTemper Technologies GmbH ...
-
bioRxiv - Molecular Biology 2022Quote: Binding assays were performed by electrophoretic mobility shift assay (EMSA) and Microscale thermophoresis (MST, NanoTemper Munich). Dilution series (1.5 fold ...
-
bioRxiv - Microbiology 2021Quote: Proteins were labeled using the Monolith NT Protein Labeling Kit RED (NanoTemper Technologies) according to the manufacturer’s protocol and using a buffer consisting of 20 mM HEPES ...
-
bioRxiv - Immunology 2019Quote: ... ILT7 purified protein was labeled using the Protein Labeling Kit RED-NHS (NanoTemper Technologies). The labeling reaction was performed according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2021Quote: ... EMCV 2A protein was labelled using the Protein Labelling Kit RED-Maleimide (NanoTemper Technologies) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2020Quote: ... Proteins were labeled using the RED-tris-NTA His tag protein labeling kit (Nanotemper Technologies). Labelled protein was added to a serial dilution of unlabeled DAG ...
-
bioRxiv - Cell Biology 2022Quote: ... Proteins were labeled using the RED-tris-NTA His tag protein-labeling kit (Nanotemper Technologies). Labeled proteins were added to a serial dilution of unlabeled DAG or liposomes prepared in binding buffer (20 mM Tris ...
-
bioRxiv - Cancer Biology 2023Quote: ... EED and JMJD3 proteins were labeled with Protein Labeling kit RED-NHS (NanoTemper, MO-L001). At least three independent experiments were performed for each binding reaction.
-
bioRxiv - Biochemistry 2019Quote: Solution protein-protein binding was monitored by microscale thermophoresis (MST) with a Monolith NT.115 (Nanotemper). The target proteins were portions of the CsoS2 NTD fused to Superfolder GFP and used at a concentration of 50 nM ...
-
bioRxiv - Biophysics 2023Quote: ... where the protein was labelled with Monolith Protein labelling kit RED-NHS (NanoTemper Technologies, Munich, Germany). In the binding assay ...
-
bioRxiv - Physiology 2023Quote: ... Purified OsABG protein was labeled using the protein labeling kit Red-NHS 2nd generation (MO-L011, Nanotemper) which dyes primary lysine residues in the protein ...
-
bioRxiv - Microbiology 2023Quote: ... Proteins were fluorescently labeled using the RED-Maleimide 2nd Generation Protein Labeling Kit (NanoTemper Technologies GmbH, Germany) as recommended by the manufacturer ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Binding assays were performed using NanoTemper NT.LabelFree instrument (NanoTemper Technologies). All experiments were carried out at room temperature in hydrophobic capillaries with 20% light-emitting diode power (fluorescence lamp intensity ...
-
bioRxiv - Plant Biology 2023Quote: ... MST assays were performed according to protocols provided by NanoTemper Technologies (20% LED power and 40% MST power) ...
-
bioRxiv - Biochemistry 2024Quote: DSF assays were performed using Tycho NT.6 (NanoTemper Technologies). RBDs were diluted to approximatively 1 mg/mL ...
-
bioRxiv - Plant Biology 2020Quote: MST assays were carried out using a Monolith NT.115 (NanoTemper) machine at the Chemical Genomics Facility of Purdue University ...
-
bioRxiv - Biophysics 2023Quote: Thermal shift assay was performed using Tycho NT.6 (NanoTemper Technologies). Envelope ectodomain SOSIPs were diluted (0.15 mg ml−1 ...
-
bioRxiv - Immunology 2023Quote: Thermal shift assay was performed using Tycho NT.6 (NanoTemper Technologies). Envelope ectodomain SOSIPs were diluted (0.15LmgLml−1 ...
-
bioRxiv - Molecular Biology 2023Quote: The affinity between RNA and protein and between proteins was measured by MST on a Monolith NT.115 (NanoTemper, Germany). Replicates represent individual measurements performed on different days using proteins from the same prep ...
-
bioRxiv - Biochemistry 2020Quote: ... His-tagged protein (Pry1 or Na-ASP-2) was fluorescently labeled using the RED-tris-NTA His tag protein labeling kit (Nanotemper Technologies). Labeled protein (Pry1 or Na-ASP-2 ...
-
bioRxiv - Biochemistry 2023Quote: ... Purified GST fusion protein were fluorescently labeled using the Monolith series Protein Labeling Kit RED-NHS 2nd Generation (Nanotemper, MO-L011) according to manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2023Quote: ... Thermal shift assay was performed on Prometheus Panta NT.48 from NanoTemper technologies by measuring the intrinsic dual-UV fluorescence change in tryptophan and tyrosine residues in proteins at emission wavelengths of k = 330 and 350 nm.The ratio of the recorded emission intensities (Em350nm/Em330nm) ...
-
bioRxiv - Biochemistry 2023Quote: ... microscale thermophoresis (MST) assays were performed on a Monolith X (Nanotemper Technologies) using standard capillaries (MO-K022 ...
-
bioRxiv - Biochemistry 2024Quote: Spectral shift assay was performed on a Monolith X instrument (NanoTemper Technologies) in parallel with MST measurements ...
-
bioRxiv - Microbiology 2021Quote: ... Phr2 and Pga4 proteins and Bull Serum Albumin (BSA) were labeled using the Monolith™ RED-NHS Protein Labeling Kit (NanoTemper Technologies) according to the manufacturer’s instructions ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: The MST assay was carried out on a Monolith NT.115 (NanoTemper Technologies). CK1γ2 was prepared at concentrations from 0.305 to 10000□nM and fluorescence dye from the Monolith His-tag Labeling kit RED-tris-NTA (NanoTemper Technologies ...
-
bioRxiv - Biochemistry 2020Quote: ... Thermally-driven protein unfolding was monitored in Tycho quartz capillaries (Nanotemper) using a Tycho NT.6 instrument (Nanotemper) ...
-
bioRxiv - Microbiology 2022Quote: ... Protein quality was verified using a Tycho NT.6 system (NanoTemper) and ACE2 activity assay kit (SensoLyte ...
-
bioRxiv - Microbiology 2019Quote: ... Hexahistidine-tagged ALIX protein was labeled by NTA-dye (NanoTemper Technologies) using manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2023Quote: ... Monolith RED-NHS 2nd generation protein labeling kit (NanoTemper Technologies GmbH) was used to label LIAS and ...
-
bioRxiv - Immunology 2022Quote: ... Protein samples (0.1 mg/mL) were loaded into glass capillaries (NanoTemper) and then subjected to a temperature gradient from 20 to 95 °C at a heating rate of 1 °C per min ...
-
bioRxiv - Immunology 2023Quote: ... Protein samples (0.1 mg/mL) were loaded into glass capillaries (NanoTemper) and then subjected to a temperature gradient from 20 to 95°C at a heating rate of 1°C per min ...
-
bioRxiv - Biophysics 2024Quote: ... CRBN protein was labelled with RED-Maleimide 2nd Generation dye (NanoTemper) optimized for degree-of-labeling of 1:1 molar ratio calculated by nanodrop ...
-
bioRxiv - Biophysics 2024Quote: ... Protein Labeling Kit RED-NHS 2nd Generation was purchased from NanoTemper Technologies (München ...
-
bioRxiv - Biochemistry 2020Quote: The microscale thermophoresis (MST) assay was performed using a Monolith NT.115 instrument (NanoTemper Technologies). For the MST assay ...