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Citations for NanoTemper :
501 - 550 of 604 citations for NT proBNP Protein Recombinant since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: The differential scanning fluorimetry (DSF) method was used to follow the thermal unfolding event of HsPepT2 with a Prometheus NT.48 device (NanoTemper Technologies, Munich, Germany). Purified HsPepT2 was diluted to 0.3 mg/mL and supplemented with decreasing amounts of Ala-Phe dipeptide in a dilution series of 13 points starting at 10 mM down to 2.4 µM ...
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bioRxiv - Biophysics 2021Quote: Binding affinities between Mdn1 MIDAS-WT or MIDAS-Y4666R and Rsa4-GFP or Ytm1-GFP were measured using a Monolith NT.115 instrument (NanoTemper Technologies, Munich, Germany). All experiments were run in binding assay buffer (50 mM Tris [pH 7.5] ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples containing 100 nM labeled PlaF were incubated for 16 h at room temperature in the dark and microscale thermophoresis (MST) experiments were performed using the Monolith® NT.115 device (NanoTemper, Munich, Germany) with the following setup ...
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bioRxiv - Biochemistry 2019Quote: ... The samples were loaded in the standard treated capillaries and measured in a NanoTemper Monolith NT.115 LabelFree instrument (NanoTemper Technologies, München, Germany). The samples were measured at LED power of 40% ...
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bioRxiv - Immunology 2021Quote: ... native or FA-cross-linked was analyzed by differential scanning fluorimetry coupled to back scattering using a Prometheus NT.48 instrument (Nanotemper Technologies, Munich, DE). Protein samples were first extensively dialyzed against PBS pH 7.4 ...
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bioRxiv - Biophysics 2022Quote: The conformational stability of HBc/4M2e on the thermal denaturation analysis was performed by differential scanning fluorimetry without additional fluorescent labels by smoothly heating the sample in a scanning fluorometer (Prometheus NT.48, NanoTemper Technologies GmbH, Germany). HBc/4M2e samples in PBS buffer solution (at concentrations of 3 mg/ml ...
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Long-Range Electrostatic Interactions Significantly Modulate the Affinity of Dynein for MicrotubulesbioRxiv - Biophysics 2021Quote: We measured the binding affinity of MTBDs (fluorescently labeled target) to microtubules (unlabeled ligand) using a Monolith NT.115 MST system (NanoTemper Technologies, GmbH, Munich, Germany). We labeled the MTBDs with a 2nd Generation RED-tris-NTA His-Tag Labeling Kit (MO-L018 ...
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bioRxiv - Microbiology 2023Quote: ... and placed in nano-DSF grade Prometheus high-sensitivity capillaries and nano-DSF measurements were carried out using a Prometheus NT.48 (Nanotemper technologies GmbH, Munchen, Germany) with a temperature ramp of 1 °C min−1 ...
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bioRxiv - Neuroscience 2023Quote: ... into 50 nM of fluorescently labeled Munc13-1 constructs in a premium-coated glass capillary using a Monolith NT.115 (Nanotemper Technologies, San Francisco, CA).
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bioRxiv - Immunology 2024Quote: The affinity of MASP-3 towards MBL and C1q in solution was determined by MicroScale Thermophoresis (MST) using a Monolith NT.115 Pico RED (Nanotemper Technologies GmbH, Munich, Germany). rMASP-3 (in-house ...
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bioRxiv - Immunology 2019Quote: ... ILT7 purified protein was labeled using the Protein Labeling Kit RED-NHS (NanoTemper Technologies). The labeling reaction was performed according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... EMCV 2A protein was labelled using the Protein Labelling Kit RED-Maleimide (NanoTemper Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were labeled using the RED-tris-NTA His tag protein labeling kit (Nanotemper Technologies). Labelled protein was added to a serial dilution of unlabeled DAG ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were labeled using the RED-tris-NTA His tag protein-labeling kit (Nanotemper Technologies). Labeled proteins were added to a serial dilution of unlabeled DAG or liposomes prepared in binding buffer (20 mM Tris ...
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bioRxiv - Cancer Biology 2023Quote: ... EED and JMJD3 proteins were labeled with Protein Labeling kit RED-NHS (NanoTemper, MO-L001). At least three independent experiments were performed for each binding reaction.
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bioRxiv - Biophysics 2023Quote: ... where the protein was labelled with Monolith Protein labelling kit RED-NHS (NanoTemper Technologies, Munich, Germany). In the binding assay ...
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bioRxiv - Physiology 2023Quote: ... Purified OsABG protein was labeled using the protein labeling kit Red-NHS 2nd generation (MO-L011, Nanotemper) which dyes primary lysine residues in the protein ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were fluorescently labeled using the RED-Maleimide 2nd Generation Protein Labeling Kit (NanoTemper Technologies GmbH, Germany) as recommended by the manufacturer ...
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bioRxiv - Biochemistry 2020Quote: ... His-tagged protein (Pry1 or Na-ASP-2) was fluorescently labeled using the RED-tris-NTA His tag protein labeling kit (Nanotemper Technologies). Labeled protein (Pry1 or Na-ASP-2 ...
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bioRxiv - Biochemistry 2023Quote: ... Purified GST fusion protein were fluorescently labeled using the Monolith series Protein Labeling Kit RED-NHS 2nd Generation (Nanotemper, MO-L011) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Phr2 and Pga4 proteins and Bull Serum Albumin (BSA) were labeled using the Monolith™ RED-NHS Protein Labeling Kit (NanoTemper Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein interaction was measured in standard capillaries (NanoTemper), 100% excitation power and medium or high MST power (IR-laser intensity) ...
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bioRxiv - Biochemistry 2020Quote: ... Thermally-driven protein unfolding was monitored in Tycho quartz capillaries (Nanotemper) using a Tycho NT.6 instrument (Nanotemper) ...
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bioRxiv - Microbiology 2019Quote: ... Hexahistidine-tagged ALIX protein was labeled by NTA-dye (NanoTemper Technologies) using manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Monolith RED-NHS 2nd generation protein labeling kit (NanoTemper Technologies GmbH) was used to label LIAS and ...
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bioRxiv - Immunology 2022Quote: ... Protein samples (0.1 mg/mL) were loaded into glass capillaries (NanoTemper) and then subjected to a temperature gradient from 20 to 95 °C at a heating rate of 1 °C per min ...
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bioRxiv - Immunology 2023Quote: ... Protein samples (0.1 mg/mL) were loaded into glass capillaries (NanoTemper) and then subjected to a temperature gradient from 20 to 95°C at a heating rate of 1°C per min ...
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bioRxiv - Biophysics 2024Quote: ... CRBN protein was labelled with RED-Maleimide 2nd Generation dye (NanoTemper) optimized for degree-of-labeling of 1:1 molar ratio calculated by nanodrop ...
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bioRxiv - Biophysics 2024Quote: ... Protein Labeling Kit RED-NHS 2nd Generation was purchased from NanoTemper Technologies (München ...
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bioRxiv - Biochemistry 2023Quote: ... the proteins were labeled with a RED-NHS Labeling Kit (NanoTemper Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Ten microliters of proteins were loaded into a capillary (PR-C002, Nanotemper) and thermal denaturation was recorded using a 1 °C/min ramp from 20 to 95 °C ...
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bioRxiv - Biochemistry 2023Quote: ... Monolith protein labeling kit RED-NHS 2nd generation was purchased from NanoTemper Technologies (Munich ...
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bioRxiv - Biochemistry 2024Quote: ... the proteins were labeled with a RED-NHS Labeling Kit (NanoTemper Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: Protein thermal stability was measured using nanoDSF on a Prometheus panta instrument (NanoTemper) (39) ...
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bioRxiv - Biochemistry 2020Quote: ... 10μL of the diluted protein was loaded into NanoDSF Grade Standard Capillaries (NanoTemper) and melting temperatures were determined using a Prometheus NT4.8 NanoDSF instrument according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2021Quote: NfrB414-745 was labeled using the Protein Labeling Kit RED-NHS (NanoTemper Technologies). The labeling reaction was performed according to the manufacturer’s instructions in the supplied labeling buffer applying a concentration of 20 μM protein at room temperature for 30 min in the dark ...
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bioRxiv - Biochemistry 2023Quote: Protein Labeling Kit RED-maleimide 2nd Generation (cat# MO-L014; NanoTemper Technologies GmbH) was used for covalent labeling of LC3A cystine residues ...
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bioRxiv - Biophysics 2022Quote: Recombinantly purified protein constructs were labelled with RED-tris NTA dye (NanoTemper Technologies GmbH) in PBS to a concentration of 100 nM ...
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bioRxiv - Cell Biology 2021Quote: ... Purified tubulin was labeled using the Monolith protein labeling kit RED-NHS (NanoTemper Technologies) and then diluted to 200 nM concentration before incubated with 2 μM eribulin for 20min at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: SAE1/SAE2 was labelled using Protein Labelling Kit RED-NHS 2nd Generation (NanoTemper Technologies). The labelling reaction was performed according to the manufacturer’s instructions in the supplied labelling buffer ...
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bioRxiv - Biophysics 2022Quote: JIP3-Nter fragments were labelled using the Protein Labelling Kit Blue-NHS (NanoTemper Technologies). The labelling reaction was performed according to the manufacturer’s instructions in the supplied labelling buffer applying a concentration of 20 μM protein (molar dye:protein ratio ≈ 3:1 ...
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bioRxiv - Plant Biology 2023Quote: ... mixed 1:1 with the labelled protein and loaded into standard capillaries (NanoTemper Technologies). MST measurements were recorded using 20 % LED power and 20 to 80 % MST power and analysed using MO ...
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bioRxiv - Biophysics 2024Quote: ... The melting temperature for characterization of protein thermal stability were done using NanoDSF (NanoTemper) with protein concentration at 0.2mg/ml ...
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bioRxiv - Biophysics 2022Quote: ... was labeled with RED-NHS (2nd Generation) dye using the Monolith Protein Labeling Kit (NanoTemper Technologies ...
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bioRxiv - Neuroscience 2023Quote: Thermal denaturation of ApoE proteins was studied by using a NanoDSF Prometheus instrument (NanoTemper, Germany). The protein samples at a concentration of 0.2 mg/ml were heated at a scan rate of 1 °C/min from 25 to 95 °C ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We used the Monolith Protein Labeling Kit RED-NHS 2nd Generation kit (NanoTemper MO- L011) to label ∼1 lysine per Fcy1 monomer with a fluorophore following the manufacturer’s instruction ...
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bioRxiv - Biochemistry 2024Quote: ... FabF protein was labeled with the RED-MALEIMIDE or RDE-NHS dye (Nanotemper Technologies, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: KDM4B was labeled with a cysteine reactive fluorescent dye (Protein Labeling Kit RED-MALEIMIDE, Nanotemper GmbH). A serial dilution of titrant was prepared in MST buffer containing 10 mM HEPES (pH 7.5) ...
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bioRxiv - Pathology 2021Quote: Spike RBD proteins were labeled using Monolith His-Tag Labeling Kit RED-tris-NTA (NanoTemper Technologies) following manufacturer protocol at 2:1 protein to dye ratio ...
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bioRxiv - Molecular Biology 2022Quote: ... 10 μM PfCoSP was labeled using NanoTemper’s Protein Labeling Kit RED-NHS (L001, NanoTemper technologies, Germany). The concentration of labeled PfCoSP protein was kept constant at a concentration of 90 nM ...