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Citations for NanoTemper :
101 - 150 of 209 citations for Mouse Vang Like Protein 2 VANGL2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Protein quality was verified using a Tycho NT.6 system (NanoTemper) and ACE2 activity assay kit (SensoLyte ...
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bioRxiv - Microbiology 2019Quote: ... Hexahistidine-tagged ALIX protein was labeled by NTA-dye (NanoTemper Technologies) using manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Protein samples (0.1 mg/mL) were loaded into glass capillaries (NanoTemper) and then subjected to a temperature gradient from 20 to 95 °C at a heating rate of 1 °C per min ...
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bioRxiv - Immunology 2023Quote: ... Protein samples (0.1 mg/mL) were loaded into glass capillaries (NanoTemper) and then subjected to a temperature gradient from 20 to 95°C at a heating rate of 1°C per min ...
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bioRxiv - Biophysics 2024Quote: ... CRBN protein was labelled with RED-Maleimide 2nd Generation dye (NanoTemper) optimized for degree-of-labeling of 1:1 molar ratio calculated by nanodrop ...
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A pectin-binding peptide with a structural and signaling role in the assembly of the plant cell wallbioRxiv - Plant Biology 2023Quote: ... protein quality was assessed using a Tycho NT.6 (NanoTemper, Munich, Germany). Labeled protein was dissolved in MST buffer (50mM Tris-HCl pH 7.8 ...
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bioRxiv - Microbiology 2023Quote: Protein thermostability was evaluated with a Prometheus NT.48 instrument (NanoTemper Technologies). Proteins at a concentration of 1 mg/mL were loaded to the grade capillaries and the intrinsic fluorescence signal was measured while temperature was increased by 1°C/min ...
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bioRxiv - Biochemistry 2023Quote: ... Ten microliters of proteins were loaded into a capillary (PR-C002, Nanotemper) and thermal denaturation was recorded using a 1 °C/min ramp from 20 to 95 °C ...
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bioRxiv - Biochemistry 2022Quote: Protein thermal stability was measured using nanoDSF on a Prometheus panta instrument (NanoTemper) (39) ...
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bioRxiv - Biochemistry 2020Quote: ... 10μL of the diluted protein was loaded into NanoDSF Grade Standard Capillaries (NanoTemper) and melting temperatures were determined using a Prometheus NT4.8 NanoDSF instrument according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2020Quote: We determined protein melting temperatures using the Prometheus NT.48 instrument (NanoTemper Technologies). Purified FabI was diluted to a concentration of 25 μM in 50 nM HEPES ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein series was loaded into Monolith NT.115 Premium Capillaries (NanoTemper Technologies) and thermophoresis was measured using MO.Control v1.6.1 software ...
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bioRxiv - Biophysics 2022Quote: Recombinantly purified protein constructs were labelled with RED-tris NTA dye (NanoTemper Technologies GmbH) in PBS to a concentration of 100 nM ...
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bioRxiv - Pathology 2021Quote: ... Protein quality was determined using a Tycho NT.6 (Figures S4 & S5) (NanoTemper Technologies). Human hnRNPC was purchased from Abnova (catalog# H00003183P01S.)
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bioRxiv - Plant Biology 2023Quote: ... mixed 1:1 with the labelled protein and loaded into standard capillaries (NanoTemper Technologies). MST measurements were recorded using 20 % LED power and 20 to 80 % MST power and analysed using MO ...
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bioRxiv - Biophysics 2024Quote: ... The melting temperature for characterization of protein thermal stability were done using NanoDSF (NanoTemper) with protein concentration at 0.2mg/ml ...
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bioRxiv - Cell Biology 2022Quote: ... employing the Monolith His-Tag Labeling Kit (RED-tris-NTA 2nd Generation kit, NanoTemper). The labeled protein was dissolved in a buffer containing PBS ...
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bioRxiv - Biochemistry 2022Quote: ... Protein folding and quality control was accomplished using nDSF on a Tycho NT.6 (NanoTemper) which monitors protein thermal unfolding using intrinsic fluorescence at 330 and 350 nm ...
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bioRxiv - Neuroscience 2023Quote: Thermal denaturation of ApoE proteins was studied by using a NanoDSF Prometheus instrument (NanoTemper, Germany). The protein samples at a concentration of 0.2 mg/ml were heated at a scan rate of 1 °C/min from 25 to 95 °C ...
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bioRxiv - Evolutionary Biology 2024Quote: We measured protein stability using nano-differential scanning fluorimetry on a Prometheus NT.48 (NanoTemper). After purification and his-tag cleavage ...
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bioRxiv - Biochemistry 2024Quote: ... FabF protein was labeled with the RED-MALEIMIDE or RDE-NHS dye (Nanotemper Technologies, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... was labeled with Monolith His-Tag Labeling Kit RED-tris-NTA kit (NanoTemper Technologies, München, Germany). Briefly ...
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bioRxiv - Biophysics 2019Quote: The stability of the different proteins was measured by nanoDSF (Prometheus NT.48, NanoTemper Technologies, GmbH). The proteins were first dialyzed against 1 l of gel filtration buffer supplemented with 5 mM EDTA overnight at 4 °C and subsequently 2x against 1 l of gel filtration buffer without EDTA overnight at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... Each protein (10 μM) was labeled with NT-647-NHS reactive dye (30 μM) (Nanotemper, Germany), which reacted efficiently with the primary amines of the proteins to form a stable dye protein conjugate ...
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bioRxiv - Molecular Biology 2022Quote: The thermal stability of the proteins was determined using Prometheus NT.48 nanoDSF instrument from NanoTemper Technologies ...
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bioRxiv - Molecular Biology 2020Quote: ... We measured the fluorescence signal from Gαi1β1γ1 by using the Monolith His-Tag Labeling Kit RED-tris-NTA 2nd Generation kit (NanoTemper Technologies). The samples were prepared in a buffer containing 20 mM sodium phosphate (pH 6.9) ...
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bioRxiv - Microbiology 2023Quote: ... + 0.5% DDM and was labeled using a His-Tag Labeling Kit RED-tris-NTA 2nd Generation Kit (NanoTemper) via the manufacturers protocol in 1.7 ml low-adhesion tubes ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The protein binding was measured by the microscale thermophoresis technique on a Monolith NT.115 instrument (Nanotemper). Protein (10 nM ...
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bioRxiv - Cell Biology 2021Quote: ... The RNA-protein mixtures were analyzed in premium capillaries using the Monolith NT.115T MST (NanoTemper Technologies). Measurements were carried out at 25 °C ...
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bioRxiv - Biochemistry 2022Quote: NanoDSF and light scattering of purified proteins were measured with a Prometheus NT.48 (Nanotemper, Munich, Germany), equipped with back-reflection optics or with a Prometheus Panta (Nanotemper ...
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bioRxiv - Biochemistry 2022Quote: ... Then the proteins were loaded onto Prometheus NT.48 nanoDSF Grade Standard Capillaries (PR-C002, NanoTemper Technologies), the fluorescence signal was recorded under a temperature gradient ranging from 25 to 90°C (incremental steps of 0.5°C min-1) ...
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bioRxiv - Biochemistry 2023Quote: Protein thermal stability was determined by differential scanning fluorimetry (nanoDSF) on Prometheus NT.48 instrument (Nanotemper Technologies) in nanoDSF grade standard capillaries filled with the samples ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Protein and compound mixtures were loaded into standard treated capillaries (MO-K022, NanoTemper Technologies GmbH, Munich, Germany). Measurements were performed on a Monolith NT.115 with blue/red filters (NanoTemper Technologies GmbH ...
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bioRxiv - Biochemistry 2024Quote: Thermal stability of proteins was analyzed using nanoscale differential scanning fluorimetry with a Prometheus NT.48 (NanoTemper) device ...
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Undruggable oncoproteins cMyc and NMyc bind to mediator of transcription with superior high affinitybioRxiv - Biochemistry 2024Quote: ... The quality of the protein was determined using Differential Scanning Fluorimetry (Prometheus NT.48, NanoTemper Technologies GmbH). The uniform labelling of KIX domain was confirmed by MALDI-TOF MS.
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bioRxiv - Biophysics 2021Quote: 6xHis-RAD51 was labelled with the Monolith His-Tag Labeling Kit RED-tris-NTA 2nd Generation kit (NanoTemper Technologies), which specifically recognizes the hexahistidine tail of the recombinant protein.
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bioRxiv - Biochemistry 2021Quote: Protein thermal stability was measured in a label-free fluorescence assay using a Prometheus NT.48 (NanoTemper, Germany). Briefly ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The protein (200 nM) was fluorescently labeled using a RED-tris-NTA 2nd generation dye (NanoTemper Technologies GmbH) and a 1:1 dye/protein molar ratio in the reaction buffer ...
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bioRxiv - Immunology 2022Quote: Thermal melting profiles of proteins were measured by differential scanning fluorimetry on a Prometheus NT.48 instrument (NanoTemper). Protein samples (0.1 mg/mL ...
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bioRxiv - Immunology 2023Quote: Thermal melting profiles of proteins were measured by differential scanning fluorimetry on a Prometheus NT.48 instrument (NanoTemper). Protein samples (0.1 mg/mL ...
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bioRxiv - Neuroscience 2023Quote: ... Control experiments included measurements of the binding of the RED-tris-NTA dye to His-DJ-1 and of a dye-labeled AptamerCy5 to AMP using a manufacturer kit (Monolith NT.115 Control Kit RED, NanoTemper).
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bioRxiv - Microbiology 2024Quote: ... and the NHS-red-tag kit (Nanotemper, Germany) was used to label ConA (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... The onset of scattering and melting temperatures based on the change of intrinsic protein fluorescence (ratio 350 nm/330 nm) were calculated in PR.ThermControl software (NanoTemper).
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bioRxiv - Biochemistry 2019Quote: The thermal stability of wild-type and variant RmtC proteins was assessed using a Tycho NT.6 instrument (NanoTemper) to ensure protein quality between different preparations of proteins and before/ after freezing ...
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bioRxiv - Plant Biology 2022Quote: Protein sample preparation and measurements were performed according to the protocol from Monolith NT (NanoTemper Technologies GmbH, Munich, Germany), employing quality controls as outlined by Sedivy 202143 ...
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bioRxiv - Molecular Biology 2022Quote: ... we evaluated the interaction of recombinant PfPHB1 and PfPHB2 proteins using Monolith NT.115 instrument (NanoTemper Technologies, Munich, Germany). Thermophoretic movement of a fluorescently labelled protein is measured by monitoring the fluorescence distribution ...
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bioRxiv - Microbiology 2021Quote: ... The labelling of NyxA was performed by covalent coupling of the lysines of the protein to a fluorophore using the RED-NHS (Nanotemper) Protein Labeling Kit ...
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bioRxiv - Biophysics 2021Quote: The thermal and chemical denaturation of the WT and the mutant 14-3-3 proteins were performed using nano-DSF (Prometheus NT.48) from Nanotemper Technologies (Munchen ...
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bioRxiv - Microbiology 2020Quote: NanoDSF Systems were conducted to measure the thermostability and aggregation of purified protein using Prometheus NT.48 instrument (NanoTemper Technologies). A 10 μL aliquot of sample diluted to a concentration of 5 mg/mL were applied to quartz capillary cassette and placed into card slot ...
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bioRxiv - Microbiology 2019Quote: ... CqsR-LBD was diluted to 20 μM and labeled using the Monolith NT Protein Labeling Ki RED-NHS (NanoTemper Technologies). Different concentrations of ethanolamine were incubated with 20 nM working stock solutions of labeled protein in the dark for 30 min at 4° C ...