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Citations for NanoTemper :
151 - 200 of 201 citations for Mouse Lactase Like Protein LCTL ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... 25 μl of a 1 mg/ml HiSiaP protein solution was prepared and 10 μl was soaked into a glass capillary (standard capillary, NanoTemper). The start and end temperature were set to 20 °C and 90 °C ...
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bioRxiv - Biochemistry 2023Quote: ... Samples containing varying concentrations of protein were loaded into glass capillaries and applied to the nanoDSF device Prometheus NT.48 (Nanotemper). The samples were heated from 20 to 90°C with a slope of 1.5°C/min and the unfolding of the proteins was observed by detecting shifts in the fluorescence at 330 and 350 nm ...
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bioRxiv - Biophysics 2022Quote: Thermal scans with measurement of the intrinsic fluorescence of the protein samples were carried out using a Tycho instrument (Nanotemper, Germany). 10 μL samples were loaded in capillaries (TY-C001 ...
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bioRxiv - Biochemistry 2022Quote: ... 5 μL of refolded protein was loaded into standard capillaries and onto the capillary tray of a Prometheus NT.48 instrument (NanoTemper Technologies) and run using a 5.0 °C/min thermal ramp at 50% LED excitation ...
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bioRxiv - Biophysics 2020Quote: ... The protein concentration was additionally measured on ND-1000 spectrophotometer (NanoDrop®) and the protein quality was evaluated by nanoDSF using a Tycho (Nanotemper).
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bioRxiv - Biochemistry 2022Quote: Protein melting profiles using 5 μM of 4B7- or 1245-Db were measured with Tycho NT.6 (NanoTemper® Technologies GmbH). Fluorescence emission intensities was measured at I350 and I330 in triplicates and its ratio plotted as a function of temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... The labeled aptamer/protein mixture is incubated for 30 min at room temperature before loading them in Monolith NT.115 Capillary (NanoTemper, Germany) and determining binding affinity using Monolith machine.
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bioRxiv - Biochemistry 2023Quote: The thermal stability of wt-JDBD and mutant proteins was measured in the Prometheus NT.48 nanoDSF (nano differential scanning fluorescence) instrument (NanoTemper Technologies). For each protein ...
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bioRxiv - Molecular Biology 2023Quote: The melting temperature Tm of a TadA protein was determined using nano differential scanning fluorimetry (nanoDSF,Nanotemper Prometheus NT.48 system) [40] ...
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bioRxiv - Cancer Biology 2023Quote: ... Thermal unfolding was followed by differential scanning fluorimetry (DSF) by measuring intrinsic protein fluorescence using a Prometheus NT.48 (NanoTemper Technologies). Aliquots (10 μl ...
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bioRxiv - Biochemistry 2024Quote: The binding affinity of each recombinant TrK13 protein for heme was evaluated by MST analysis using the Monolith NT.115 instrument (NanoTemper Technologies). The recombinant TrK13 proteins (50 nM ...
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bioRxiv - Biophysics 2023Quote: ... and the intrinsic protein fluorescence was recorded at 330 and 350 nm using the Prometheus NT.Plex nanoDSF device (NanoTemper, Munich, Germany).
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bioRxiv - Cancer Biology 2024Quote: ... 90 µl of 200 nM protein mixed 1:1 with 100 nM RED-tris-NTA 2nd generation dye (MO-L018, Nanotemper Technologies) and incubated at room temperature for 30 minutes ...
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bioRxiv - Microbiology 2020Quote: ... Monolith His-Tag Labeling kit (MO-L018) was from NanoTemper Technologies.
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bioRxiv - Biochemistry 2021Quote: Fluorescence labeling of the target protein (MDH) was performed by Red N-hydroxy succinimide (NHS) 2nd generation (NanoTemper Technologies GmbH, München, Germany) following the manufacturer’s protocol ...
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bioRxiv - Biophysics 2021Quote: The thermal unfolding experiments of the IgLC samples as a function of protein and denaturant concentration were performed with a Prometheus Panta instrument (Nanotemper, Munich, Germany). This is a microcapillary-based (10 μl sample per capillary ...
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bioRxiv - Plant Biology 2022Quote: The purified protein in PBS at a concentration of ∼16 µM was loaded into capillaries (nanoDSF grade standard, NanoTemper GmbH, Munich, Germany) and a melting curve was determined by using the nanoDSF Prometheus NT.48 instrument (NanoTemper GmbH ...
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bioRxiv - Biochemistry 2023Quote: ... Each protein dilution was mixed with one volume of labeled DNA and filled into Monolith NT standard treated capillaries (NanoTemper Technologies GmbH). Thermophoresis was measured using a Monolith NT.115 instrument (NanoTemper Technologies GmbH ...
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bioRxiv - Cell Biology 2024Quote: MST measurements were carried out using premium-coated capillaries to reduce non-specific interaction of the proteins with the glass surface on a Monolith NT.115 red-blue (Nanotemper, Munich, Germany), as described previously (Zuidema et al. ...
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bioRxiv - Microbiology 2019Quote: The binding affinities of anti-CRISPR proteins to SpyCas9 were calculated using microscale thermophoresis (MST) on the Monolith NT.115 instrument (NanoTemper Technologies GmbH, Munich, Germany). For AcrIIA1/AcrIIA2b.3 with WT or mutant Cas9-gRNA complexes ...
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bioRxiv - Plant Biology 2022Quote: ... 50 nM of protein sample in PBS together with indicated concentrations of nucleotides were loaded into standard Monolith NT.115 capillaries (NanoTemper Technologies GmbH, Munich, Germany). MST measurement was performed using the Monolith NT.115 instrument (NanoTemper Technologies GmbH ...
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bioRxiv - Cell Biology 2023Quote: His-Tag labeling Kit RED-tris-NTA 2nd generation (MO-L018, Nanotemper) was used to label His-tagged Bax purchased from Creative BioMart (Catalog No ...
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bioRxiv - Biochemistry 2023Quote: ... using a His-tag Labeling Kit (NanoTemper Technologies, cat. no. MO-L018). For measurements using CBP30 ...
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bioRxiv - Biochemistry 2021Quote: Fluorophore-labeled protein was incubated with various ligands at room temperature for 15 minutes before measuring on Monolith NT.115 (NanoTemper, 80% LED power, 40% MST power). For competition assays ...
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bioRxiv - Molecular Biology 2021Quote: TCNB4 was covalently labeled with Red-NHS using the Monolith NT kit (Nanotemper technologies) and used in the cellular binding and uptake studies ...
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bioRxiv - Biochemistry 2021Quote: ... It was fluorescently labelled with the RED-NHS 2nd Generation kit (NanoTemper Technologies GmbH), and desalted to the reaction buffer (10 mM MES ...
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bioRxiv - Molecular Biology 2020Quote: Purified SecB was randomly labelled using an NT-647-NHS labelling kit (Nanotemper Technologies). 160 nM NT-647-labelled SecB was incubated with the indicated concentrations of unlabelled AscA or AscAΔMBD in buffer (20 mM potassium HEPES [pH 7.5] ...
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bioRxiv - Biochemistry 2024Quote: ... was labeled using the Monolith His-Tag Labeling Kit Red-tris-NTA (NanoTemper Technologies) and diluted in MST buffer (NanoTemper Technologies ...
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bioRxiv - Molecular Biology 2020Quote: ... PrgU-His was labeled with the RED 2nd generation His-tag labeling kit (NanoTemper Technologies) according to the manufacturer’s instructions with a final concentration in the assay of 50 nM (molar dye ...
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bioRxiv - Cell Biology 2022Quote: ... Monolith His-Tag Labeling Kit RED-tris-NTA 2nd Generation (NanoTemper Technologies, catalog #: MO-L018) was used to label recombinant His-α1-ERD (MyBioSource ...
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bioRxiv - Cell Biology 2023Quote: His6-CTxA1 was labeled using Monolith NT His-Tag Labeling Kit RED-tris-NTA (NanoTemper). CTxB labeled with Alexa Fluor 647 (Invitrogen ...
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bioRxiv - Biophysics 2020Quote: ... zRETECD was labelled with NHS-RED 2nd generation dye (Amine Reactive) using the labelling kit (Nanotemper). A 1:1 serial dilution of unlabelled zGFRα1D1-3-zGDNFmat ...
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bioRxiv - Biochemistry 2024Quote: ... coli was labeled using the Monolith Lys-Tag Labeling Kit RED-NHS second generation (NanoTemper Technologies), according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: ... cMyBP-C constructs were fluorescently labeled via His-tag using a commercially available kit (NanoTemper Technologies). Unlabeled sS1 or 15-hep myosin was titrated against 84 nM fluorescently labeled C3 or C0C7 protein ...
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bioRxiv - Biochemistry 2024Quote: ... was labeled using the Monolith His-Tag Labeling Kit RED-Tris-NTA second generation (NanoTemper Technologies), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: His6-tagged caspases were labelled using the His-tag labelling Kit RED-tris-NTA 2nd generation (NanoTemper). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... as recommended by the Monolith His-Tag Labeling Kit RED-tris-NTA protocol (NanoTemper Technologies, MO-L008). The binding buffer used for reactions [50 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Immunology 2023Quote: ... were fluorescently labeled using His-Tag Labeling Kit RED-Tris-NTA 2nd Generation (NanoTemper, Cat# MO-L018). The proteins (at fixed concentration ...
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bioRxiv - Genetics 2024Quote: ... IS621 recombinase was labeled for MST using the RED-MALEIMIDE 2nd Generation cysteine reactive kit (NanoTemper technologies) as per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... C0-C7 was fluorescently labeled with a commercial His-tag labeling kit according to manufacturer instructions (Nanotemper technologies). His-labeled C0-C7 fluorescence was measured by a red LED at 60-90% excitation power (RED filter ...
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bioRxiv - Biochemistry 2020Quote: ... S1-His or RBD-His were labelled using Monolith NTTM His-Tag Labeling Kit RED-tris-NTA (Nanotemper) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2019Quote: ... was labelled with 100 μM Monolith NTTM His-Tag Labeling kit RED-tris-NTA (L008, NANOTEMPER, Munich, Germany) 30 min at room temperature (RT ...
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bioRxiv - Plant Biology 2022Quote: ... The N-terminal His-tag was labelled with the RED-tris-NTA kit (NanoTemper Technologies GmbH, Munich, Germany). After 10 minutes incubation on ice ...
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bioRxiv - Biophysics 2020Quote: ... zRETECD P291-Q296;AAG was labelled with NHS-RED 2nd generation dye (Amine Reactive) using the labelling kit (Nanotemper), and the procedure was carried out as above with zRETECD P291-Q296;AAG -NHS-RED (50 nM ...
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bioRxiv - Neuroscience 2023Quote: ... was labelled via His-tag with Monolith His-Tag Labeling Kit RED-tris-NTA 2nd Generation (NanoTemper Technologies GmbH). The labelled complex was diluted in MST buffer (10 mM Na-phosphate buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... 650 nm fluorescent dye using the Monolith NT RED-NHS lysine labeling kit according to manufacturer’s protocol (NanoTemper, Munich, Germany). RED labeled untagged sP36 at 100 nM and His6-GlnK1 in 16 different concentrations ranging from 7.5 µM to 0.23 nM or 10 nM of sP36-RED and AmtB1-His6 in 16 dilutions ranging from 12.6 µM to 0.38 nM (all concentrations based on monomeric molecular mass ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... CK1γ2 was prepared at concentrations from 0.305 to 10000□nM and fluorescence dye from the Monolith His-tag Labeling kit RED-tris-NTA (NanoTemper Technologies) were added to concentrations of 80□nM and 5□nM ...
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bioRxiv - Microbiology 2019Quote: Three independent MST experiments were performed with His-tagged BspC labelled using the Monolith His-Tag Labeling Kit RED-tris-NTA 2nd Generation (NanoTemper Technologies) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Labeling was performed by incubating PlaF with 15 μL dye (440 μM stock solution) for 2.5 h using the NHS labeling Kit (NanoTemper, Munich, Germany). PlaF was then transferred into a purification buffer using PD-10 columns ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... for 30 min with 50 nM RED-tris-NTA 2nd Generation dye from the Monolith His-Tag Labeling Kit (Cat.#MO-L018; NanoTemper Technologies). The labeling reaction mixture was centrifuged for 10 min at 4°C and 15,000 x g to remove protein aggregates ...