Labshake search
Citations for NanoTemper :
151 - 184 of 184 citations for Microtubule Associated Protein 1 Light Chain 3 Gamma MAP1LC3C Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: The melting temperature Tm of a TadA protein was determined using nano differential scanning fluorimetry (nanoDSF,Nanotemper Prometheus NT.48 system) [40] ...
-
The activator domain of bacterial collagenases drives collagen recognition, unwinding and processingbioRxiv - Biochemistry 2023Quote: ... ColG variants were labeled using the Monolith NT™ Protein Labeling Kit RED-NHS 2nd Generation Amine reactive (NanoTemper Technologies, Germany). After labeling ...
-
bioRxiv - Cancer Biology 2023Quote: ... Thermal unfolding was followed by differential scanning fluorimetry (DSF) by measuring intrinsic protein fluorescence using a Prometheus NT.48 (NanoTemper Technologies). Aliquots (10 μl ...
-
bioRxiv - Biophysics 2023Quote: ... and the intrinsic protein fluorescence was recorded at 330 and 350 nm using the Prometheus NT.Plex nanoDSF device (NanoTemper, Munich, Germany).
-
bioRxiv - Biochemistry 2024Quote: The binding affinity of each recombinant TrK13 protein for heme was evaluated by MST analysis using the Monolith NT.115 instrument (NanoTemper Technologies). The recombinant TrK13 proteins (50 nM ...
-
bioRxiv - Plant Biology 2024Quote: ... Purified insect cell-expressed RACK1A was fluorescently labeled with Protein Labeling Kit RED-NHS 2nd Generation (NanoTemper Technologies GmbH MO-L011) via amine conjugation ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: Purified TRIM21 PRYSPRY domain was labeled using Protein Labeling Kit RED-NHS 2nd Generation (MO-L011, NanoTemper Technologies GmbH, Munich, Germany). Labeled protein was diluted to 50 nM in PBST (137 mM NaCl ...
-
bioRxiv - Biochemistry 2020Quote: 50 µg synthesized sP26 (Davids Biotechnologie, Regensburg, Germany) was fluorescently labeled with the Monolith NT Protein Labeling Kit RED (NanoTemper, Munich, Germany) according to manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2019Quote: BRCA1 tBRCT wild type or mutant domains used in the assay were labeled with NT-647-NHS fluorescent dye using the Monolith NT™ Protein Labeling Kit (NanoTemper Technologies). Assays were carried out in 20mM Tris buffer ...
-
bioRxiv - Biochemistry 2021Quote: Fluorescence labeling of the target protein (MDH) was performed by Red N-hydroxy succinimide (NHS) 2nd generation (NanoTemper Technologies GmbH, München, Germany) following the manufacturer’s protocol ...
-
bioRxiv - Biophysics 2021Quote: The thermal unfolding experiments of the IgLC samples as a function of protein and denaturant concentration were performed with a Prometheus Panta instrument (Nanotemper, Munich, Germany). This is a microcapillary-based (10 μl sample per capillary ...
-
bioRxiv - Plant Biology 2022Quote: The purified protein in PBS at a concentration of ∼16 µM was loaded into capillaries (nanoDSF grade standard, NanoTemper GmbH, Munich, Germany) and a melting curve was determined by using the nanoDSF Prometheus NT.48 instrument (NanoTemper GmbH ...
-
bioRxiv - Molecular Biology 2023Quote: ... 20 µM His6-SUMO-AGO1-NcGN was orthogonally labeled on Cystein-192 using the Monolith NT™ Protein Labeling Kit Red – Maleimide (NanoTemper Technologies). The labeling was performed according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2024Quote: MST measurements were carried out using premium-coated capillaries to reduce non-specific interaction of the proteins with the glass surface on a Monolith NT.115 red-blue (Nanotemper, Munich, Germany), as described previously (Zuidema et al. ...
-
bioRxiv - Biochemistry 2023Quote: ... Each protein dilution was mixed with one volume of labeled DNA and filled into Monolith NT standard treated capillaries (NanoTemper Technologies GmbH). Thermophoresis was measured using a Monolith NT.115 instrument (NanoTemper Technologies GmbH ...
-
bioRxiv - Biophysics 2020Quote: ... PD-1 and its mutant were fluorescently labelled according the standard labeling protocol of the NanoTemper Protein Labeling Kit RED-NHS (L001-NanoTemper Technologies, Munich, Germany). In MST experiments ...
-
bioRxiv - Neuroscience 2021Quote: ... Recombinant human FTO protein was labeled with His-tag using Monolith His-Tag Labeling Kit RED-tris-NTA (NanoTemper Technologies GmbH; MO-L008). The labelled FTO protein (target ...
-
bioRxiv - Biochemistry 2023Quote: The BD5.1 protein was labelled with the Monolith His-Tag Labeling Kit (RED-tris-NTA 2nd Generation kit, NanoTemper Technologies, Catalog# MO-L018) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2019Quote: The binding affinities of anti-CRISPR proteins to SpyCas9 were calculated using microscale thermophoresis (MST) on the Monolith NT.115 instrument (NanoTemper Technologies GmbH, Munich, Germany). For AcrIIA1/AcrIIA2b.3 with WT or mutant Cas9-gRNA complexes ...
-
bioRxiv - Plant Biology 2022Quote: ... 50 nM of protein sample in PBS together with indicated concentrations of nucleotides were loaded into standard Monolith NT.115 capillaries (NanoTemper Technologies GmbH, Munich, Germany). MST measurement was performed using the Monolith NT.115 instrument (NanoTemper Technologies GmbH ...
-
bioRxiv - Biophysics 2024Quote: The (I91)2-ClfAN2N3-(I91)2 polyprotein was fluorescently labeled by utilizing the commercial protein Labeling Kit RED-NHS 2nd Generation (Nanotemper Technologies GmbH, Munich, Germany). The dye carries a reactive NHS-ester group that reacts with primary amines in the protein to form a covalent bond ...
-
bioRxiv - Biochemistry 2020Quote: ... histidine tagged HMGB1 was labeled by the his-tag specific NT-647 dye (Monolith NTTM Protein Labelling Kit RED-NHS, NanoTemper® Technologies GmbH, Mu□nchen, Germany), for 30 minutes at room temperature ...
-
bioRxiv - Biochemistry 2021Quote: Fluorophore-labeled protein was incubated with various ligands at room temperature for 15 minutes before measuring on Monolith NT.115 (NanoTemper, 80% LED power, 40% MST power). For competition assays ...
-
bioRxiv - Biophysics 2020Quote: ... 1 mM CaCl2 and 0.05 % (v/v) Tween-20 using a Nanotemper Monolith NT.115 (Nanotemper). To measure the affinity of zGFRα1D1-3-zGDNFmat ...
-
bioRxiv - Neuroscience 2023Quote: ... Fluorescent NbSyt1 was incubated with different dilutions of purified Syt-1(97-421) using Premium Coated Capillaries (NanoTemper). For operation of the instrument and evaluation of affinity data ...
-
bioRxiv - Biophysics 2020Quote: Thermal unfolding of BG505-SOSIP was determined with a heating ramp of 1°C/min and 20% sensitivity setting using high-sensitivity capillaries (NanoTemper Technologies ...
-
bioRxiv - Neuroscience 2023Quote: ... Control experiments included measurements of the binding of the RED-tris-NTA dye to His-DJ-1 and of a dye-labeled AptamerCy5 to AMP using a manufacturer kit (Monolith NT.115 Control Kit RED, NanoTemper).
-
bioRxiv - Biophysics 2021Quote: The data was analyzed and the Ktet (Eq. 1) or EC50 (Eq. 2) calculated using the MO affinity analysis software (NanoTemper, Germany), considering a 1:1 model.
-
bioRxiv - Microbiology 2019Quote: ... The binding affinities were determined to fit the data points to a quadratic equation (equation 1) using non-linear regression using MO Affinity software (NanoTemper Technologies), Prism (GraphPad Inc ...
-
bioRxiv - Biophysics 2020Quote: The thermal stability of target proteins in different buffers and the potential stabilizing effects of ligands was evaluated at 1 μM using a direct thermal shift assay using a Tycho NT6 instrument (Nanotemper Technologies), monitoring intrinsic protein fluorescence at 330 and 350 nm during a thermal ramp from 35 °C to 95 °C ...
-
bioRxiv - Cell Biology 2019Quote: ... 10 ⌈L of 1 mg/mL ADCs were loaded into standard capillaries and mounted in a Prometheus NT.48 (Nanotemper Technologies, Germany) with excitation near UV ...
-
bioRxiv - Cell Biology 2023Quote: ... The interaction of NRP-1 structural variants with FOL-peptides was measured using a Monolith NT.115 [NT.115Pico/NT.LabelFree] instrument (NanoTemper Technologies, Munich, Germany). For more details see supplementary materials and methods.
-
bioRxiv - Cell Biology 2021Quote: ... 10 μl of the protein samples at 10 μM were measured in triplicates with a temperature ramp set to 1 °C/min from 20 °C-80 °C using standard treated capillaries (NanoTemper Technologies (#PR-C002)) ...
-
bioRxiv - Neuroscience 2023Quote: ... into 50 nM of fluorescently labeled Munc13-1 constructs in a premium-coated glass capillary using a Monolith NT.115 (Nanotemper Technologies, San Francisco, CA).