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Citations for NanoTemper :
51 - 100 of 195 citations for Mayaro Virus E2 Envelope Protein Mouse Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: Protein-protein interactions were monitored by MST using a Monolith NT.115 instrument (Nanotemper Technologies). His6-MyoVa GTD was labeled using RED-NHS 2nd Generation (Amine Reactive ...
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bioRxiv - Cancer Biology 2023Quote: ... EED and JMJD3 proteins were labeled with Protein Labeling kit RED-NHS (NanoTemper, MO-L001). At least three independent experiments were performed for each binding reaction.
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bioRxiv - Biochemistry 2019Quote: Solution protein-protein binding was monitored by microscale thermophoresis (MST) with a Monolith NT.115 (Nanotemper). The target proteins were portions of the CsoS2 NTD fused to Superfolder GFP and used at a concentration of 50 nM ...
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bioRxiv - Biochemistry 2022Quote: Protein–protein interactions were monitored by microscale thermophoresis using a Monolith NT.115 instrument (Nanotemper Technologies). His LRRK2 Armadillo (1-552) ...
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bioRxiv - Biochemistry 2023Quote: Protein–protein interactions were monitored by microscale thermophoresis using a Monolith NT.115 instrument (NanoTemper Technologies). His LRRK2 Armadillo (1–552 ...
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bioRxiv - Biophysics 2023Quote: ... where the protein was labelled with Monolith Protein labelling kit RED-NHS (NanoTemper Technologies, Munich, Germany). In the binding assay ...
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bioRxiv - Microbiology 2022Quote: ... Protein-protein interactions were analyzed at 20°C by MST on a Monolith NT.115 (NanoTemper technologies) using standard capillaries ...
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bioRxiv - Physiology 2023Quote: ... Purified OsABG protein was labeled using the protein labeling kit Red-NHS 2nd generation (MO-L011, Nanotemper) which dyes primary lysine residues in the protein ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were fluorescently labeled using the RED-Maleimide 2nd Generation Protein Labeling Kit (NanoTemper Technologies GmbH, Germany) as recommended by the manufacturer ...
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bioRxiv - Molecular Biology 2023Quote: The affinity between RNA and protein and between proteins was measured by MST on a Monolith NT.115 (NanoTemper, Germany). Replicates represent individual measurements performed on different days using proteins from the same prep ...
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bioRxiv - Biochemistry 2023Quote: ... Purified GST fusion protein were fluorescently labeled using the Monolith series Protein Labeling Kit RED-NHS 2nd Generation (Nanotemper, MO-L011) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Phr2 and Pga4 proteins and Bull Serum Albumin (BSA) were labeled using the Monolith™ RED-NHS Protein Labeling Kit (NanoTemper Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein interaction was measured in standard capillaries (NanoTemper), 100% excitation power and medium or high MST power (IR-laser intensity) ...
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bioRxiv - Immunology 2022Quote: Protein-protein interactions were analyzed via microscale thermophoresis on a Monolith NT.115 instrument equipped with green/red filters (NanoTemper Technologies, Munich, Germany). Measurements were performed at 25°C at both 40% and 80% MST power ...
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bioRxiv - Biochemistry 2020Quote: ... Thermally-driven protein unfolding was monitored in Tycho quartz capillaries (Nanotemper) using a Tycho NT.6 instrument (Nanotemper) ...
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bioRxiv - Microbiology 2022Quote: ... Protein quality was verified using a Tycho NT.6 system (NanoTemper) and ACE2 activity assay kit (SensoLyte ...
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bioRxiv - Microbiology 2019Quote: ... Hexahistidine-tagged ALIX protein was labeled by NTA-dye (NanoTemper Technologies) using manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Monolith RED-NHS 2nd generation protein labeling kit (NanoTemper Technologies GmbH) was used to label LIAS and ...
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bioRxiv - Immunology 2022Quote: ... Protein samples (0.1 mg/mL) were loaded into glass capillaries (NanoTemper) and then subjected to a temperature gradient from 20 to 95 °C at a heating rate of 1 °C per min ...
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bioRxiv - Immunology 2023Quote: ... Protein samples (0.1 mg/mL) were loaded into glass capillaries (NanoTemper) and then subjected to a temperature gradient from 20 to 95°C at a heating rate of 1°C per min ...
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bioRxiv - Biophysics 2024Quote: ... CRBN protein was labelled with RED-Maleimide 2nd Generation dye (NanoTemper) optimized for degree-of-labeling of 1:1 molar ratio calculated by nanodrop ...
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bioRxiv - Biophysics 2024Quote: ... Protein Labeling Kit RED-NHS 2nd Generation was purchased from NanoTemper Technologies (München ...
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bioRxiv - Biophysics 2021Quote: ... or Monolith NT™ (Protein Labeling Kit Blue-NHS, NanoTemper GmbH, Germany). The labeling was achieved by incubating 115 μM of protein and 350 μM of dye (465 μl reaction volume ...
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A pectin-binding peptide with a structural and signaling role in the assembly of the plant cell wallbioRxiv - Plant Biology 2023Quote: ... protein quality was assessed using a Tycho NT.6 (NanoTemper, Munich, Germany). Labeled protein was dissolved in MST buffer (50mM Tris-HCl pH 7.8 ...
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bioRxiv - Microbiology 2023Quote: Protein thermostability was evaluated with a Prometheus NT.48 instrument (NanoTemper Technologies). Proteins at a concentration of 1 mg/mL were loaded to the grade capillaries and the intrinsic fluorescence signal was measured while temperature was increased by 1°C/min ...
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bioRxiv - Biochemistry 2023Quote: ... the proteins were labeled with a RED-NHS Labeling Kit (NanoTemper Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Ten microliters of proteins were loaded into a capillary (PR-C002, Nanotemper) and thermal denaturation was recorded using a 1 °C/min ramp from 20 to 95 °C ...
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bioRxiv - Biochemistry 2023Quote: ... Monolith protein labeling kit RED-NHS 2nd generation was purchased from NanoTemper Technologies (Munich ...
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bioRxiv - Biochemistry 2024Quote: ... the proteins were labeled with a RED-NHS Labeling Kit (NanoTemper Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: Protein thermal stability was measured using nanoDSF on a Prometheus panta instrument (NanoTemper) (39) ...
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bioRxiv - Biochemistry 2020Quote: ... 10μL of the diluted protein was loaded into NanoDSF Grade Standard Capillaries (NanoTemper) and melting temperatures were determined using a Prometheus NT4.8 NanoDSF instrument according to the manufacturer’s instruction ...
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bioRxiv - Biochemistry 2022Quote: ... 150 mM NaCl using the Monolith NT Protein Labelling Kit RED (NanoTemper Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: We determined protein melting temperatures using the Prometheus NT.48 instrument (NanoTemper Technologies). Purified FabI was diluted to a concentration of 25 μM in 50 nM HEPES ...
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bioRxiv - Microbiology 2021Quote: NfrB414-745 was labeled using the Protein Labeling Kit RED-NHS (NanoTemper Technologies). The labeling reaction was performed according to the manufacturer’s instructions in the supplied labeling buffer applying a concentration of 20 μM protein at room temperature for 30 min in the dark ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein series was loaded into Monolith NT.115 Premium Capillaries (NanoTemper Technologies) and thermophoresis was measured using MO.Control v1.6.1 software ...
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bioRxiv - Cell Biology 2023Quote: IRE1αLD was labelled using the Monolith NT protein labelling Kit Red-Maleimide (NanoTemper). The concentration of the labelled IRE1αLD was kept constant at 10 nM ...
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bioRxiv - Biochemistry 2023Quote: Protein Labeling Kit RED-maleimide 2nd Generation (cat# MO-L014; NanoTemper Technologies GmbH) was used for covalent labeling of LC3A cystine residues ...
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bioRxiv - Biophysics 2022Quote: Recombinantly purified protein constructs were labelled with RED-tris NTA dye (NanoTemper Technologies GmbH) in PBS to a concentration of 100 nM ...
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bioRxiv - Cell Biology 2021Quote: ... Purified tubulin was labeled using the Monolith protein labeling kit RED-NHS (NanoTemper Technologies) and then diluted to 200 nM concentration before incubated with 2 μM eribulin for 20min at room temperature ...
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bioRxiv - Pathology 2021Quote: ... Protein quality was determined using a Tycho NT.6 (Figures S4 & S5) (NanoTemper Technologies). Human hnRNPC was purchased from Abnova (catalog# H00003183P01S.)
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bioRxiv - Molecular Biology 2022Quote: SAE1/SAE2 was labelled using Protein Labelling Kit RED-NHS 2nd Generation (NanoTemper Technologies). The labelling reaction was performed according to the manufacturer’s instructions in the supplied labelling buffer ...
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bioRxiv - Biophysics 2022Quote: JIP3-Nter fragments were labelled using the Protein Labelling Kit Blue-NHS (NanoTemper Technologies). The labelling reaction was performed according to the manufacturer’s instructions in the supplied labelling buffer applying a concentration of 20 μM protein (molar dye:protein ratio ≈ 3:1 ...
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bioRxiv - Plant Biology 2023Quote: ... mixed 1:1 with the labelled protein and loaded into standard capillaries (NanoTemper Technologies). MST measurements were recorded using 20 % LED power and 20 to 80 % MST power and analysed using MO ...
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bioRxiv - Biophysics 2024Quote: ... The melting temperature for characterization of protein thermal stability were done using NanoDSF (NanoTemper) with protein concentration at 0.2mg/ml ...
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bioRxiv - Molecular Biology 2021Quote: ... Nanobodies were labeled using the lysine reactive Monolith NT Protein Labeling kit RED-NHS (NanoTemper). As TCNB11 has a lysine residue in CDR2 ...
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bioRxiv - Biophysics 2022Quote: ... was labeled with RED-NHS (2nd Generation) dye using the Monolith Protein Labeling Kit (NanoTemper Technologies ...
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bioRxiv - Biochemistry 2022Quote: ... Protein folding and quality control was accomplished using nDSF on a Tycho NT.6 (NanoTemper) which monitors protein thermal unfolding using intrinsic fluorescence at 330 and 350 nm ...
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bioRxiv - Biochemistry 2021Quote: ... g5Rp was first labeled using the Monolith NT Protein Labeling Kit RED-NHS (NanoTemper Technologies), and the labeled protein was then diluted to 20 nM with buffer containing 50 mM HEPES ...
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bioRxiv - Neuroscience 2023Quote: Thermal denaturation of ApoE proteins was studied by using a NanoDSF Prometheus instrument (NanoTemper, Germany). The protein samples at a concentration of 0.2 mg/ml were heated at a scan rate of 1 °C/min from 25 to 95 °C ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We used the Monolith Protein Labeling Kit RED-NHS 2nd Generation kit (NanoTemper MO- L011) to label ∼1 lysine per Fcy1 monomer with a fluorophore following the manufacturer’s instruction ...