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Citations for NanoTemper :
151 - 195 of 195 citations for Mayaro Virus E2 Envelope Protein Human Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: The purified HBsAg was purchased from ProSpec (hbs-872-c) and was labeled by RED-NHS dye (monolith protein labeling kit, MO-L011, NanoTemper) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2020Quote: NanoDSF Systems were conducted to measure the thermostability and aggregation of purified protein using Prometheus NT.48 instrument (NanoTemper Technologies). A 10 μL aliquot of sample diluted to a concentration of 5 mg/mL were applied to quartz capillary cassette and placed into card slot ...
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bioRxiv - Microbiology 2019Quote: ... CqsR-LBD was diluted to 20 μM and labeled using the Monolith NT Protein Labeling Ki RED-NHS (NanoTemper Technologies). Different concentrations of ethanolamine were incubated with 20 nM working stock solutions of labeled protein in the dark for 30 min at 4° C ...
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bioRxiv - Molecular Biology 2021Quote: ... His-Cdc42 was labeled following the instructions of the MO-L001 Monolith NT Protein Labeling Kit RED – NHS (NanoTemper Technologies). Labelled protein concentration in the binding reactions was 10 nM while compounds concentration was either 50 or 100 μM ...
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bioRxiv - Biochemistry 2022Quote: The amine residues of purified KsgA were fluorescently labeled with NHS red using the Protein Labeling Kit RED-NHS 2nd Generation (Cat # MO-L011 Nanotemper). The labeling reaction was performed according to the manufacturer’s protocol by mixing KsgA at a final concentration of 20 μM with a 3-fold molar excess of dye at room temperature for 30 minutes in the dark ...
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bioRxiv - Molecular Biology 2022Quote: ... The TPR-CHAT and its mutants were fluorescence-labeled using the Protein Labeling Kit RED-NHS 2nd Generation (NanoTemper Technologies) at 10 μM ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Monitoring of the ITF at 330 nm and 350 nm during protein thermal denaturation was carried out in a Prometheus NT.48 instrument from NanoTemper Technologies with an excitation wavelength of 280 nm ...
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The activator domain of bacterial collagenases drives collagen recognition, unwinding and processingbioRxiv - Biochemistry 2023Quote: ColG-variants were labeled using the Monolith NT™ Protein Labeling Kit RED-NHS 2nd Generation Amine reactive (NanoTemper, Germany) and the yield of labelled protein and the degree of labelling were determined according to the manufacturer’s manual ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Monitoring of the ITF at 330 nm and 350 nm during protein thermal denaturation was carried out in a Prometheus NT.48 instrument from NanoTemper Technologies (Munchen ...
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bioRxiv - Biochemistry 2023Quote: ... 25 μl of a 1 mg/ml HiSiaP protein solution was prepared and 10 μl was soaked into a glass capillary (standard capillary, NanoTemper). The start and end temperature were set to 20 °C and 90 °C ...
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bioRxiv - Biochemistry 2023Quote: ... Samples containing varying concentrations of protein were loaded into glass capillaries and applied to the nanoDSF device Prometheus NT.48 (Nanotemper). The samples were heated from 20 to 90°C with a slope of 1.5°C/min and the unfolding of the proteins was observed by detecting shifts in the fluorescence at 330 and 350 nm ...
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bioRxiv - Neuroscience 2024Quote: We followed the manufacturer’s instructions to label APOE3 and APOE4 using the Monolith protein labeling kit RED-NHS 2nd generation (NanoTemper Technologies). In summary ...
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bioRxiv - Immunology 2022Quote: ... Germany) and MST-Red-MIF was prepared using the Monolith Protein Labeling Kit RED-NHS 2nd Generation from NanoTemper (Munich, Germany), following the manufacturers’ instructions ...
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bioRxiv - Biophysics 2022Quote: Thermal scans with measurement of the intrinsic fluorescence of the protein samples were carried out using a Tycho instrument (Nanotemper, Germany). 10 μL samples were loaded in capillaries (TY-C001 ...
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bioRxiv - Biochemistry 2022Quote: ... 5 μL of refolded protein was loaded into standard capillaries and onto the capillary tray of a Prometheus NT.48 instrument (NanoTemper Technologies) and run using a 5.0 °C/min thermal ramp at 50% LED excitation ...
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bioRxiv - Biophysics 2022Quote: His-tagged NCTD was fluorescently labelled with NT-647 amine reactive dye from the Monolith NT Protein Labelling kit RED-NHS (NanoTemper Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... RBD was labeled with the RED-NHS 2nd generation Monolith Protein Labeling Kit according to the manufacturer’s instructions (Nanotemper; MO-L011). The fluorescent group was conjugated to RBD through primary amines ...
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bioRxiv - Biophysics 2020Quote: ... The protein concentration was additionally measured on ND-1000 spectrophotometer (NanoDrop®) and the protein quality was evaluated by nanoDSF using a Tycho (Nanotemper).
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bioRxiv - Biochemistry 2022Quote: Protein melting profiles using 5 μM of 4B7- or 1245-Db were measured with Tycho NT.6 (NanoTemper® Technologies GmbH). Fluorescence emission intensities was measured at I350 and I330 in triplicates and its ratio plotted as a function of temperature ...
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bioRxiv - Microbiology 2022Quote: ... were labeled with the Monolith Protein Labeling Kit RED-NHS 2nd generation according to the recommendations of the manufacturer (NanoTemper Technologies). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... The labeled aptamer/protein mixture is incubated for 30 min at room temperature before loading them in Monolith NT.115 Capillary (NanoTemper, Germany) and determining binding affinity using Monolith machine.
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The activator domain of bacterial collagenases drives collagen recognition, unwinding and processingbioRxiv - Biochemistry 2023Quote: ... ColG variants were labeled using the Monolith NT™ Protein Labeling Kit RED-NHS 2nd Generation Amine reactive (NanoTemper Technologies, Germany). After labeling ...
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bioRxiv - Biochemistry 2023Quote: ... His LRRK2 Armadillo (1–552) K439E and E240R were labeled using RED-NHS 2nd Generation (Amine Reactive) Protein Labeling Kit (NanoTemper Technologies). For all experiments ...
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bioRxiv - Biochemistry 2023Quote: The thermal stability of wt-JDBD and mutant proteins was measured in the Prometheus NT.48 nanoDSF (nano differential scanning fluorescence) instrument (NanoTemper Technologies). For each protein ...
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bioRxiv - Biophysics 2023Quote: The recombinant RAD52 FL protein was labeled with the Monolith labeling kit RED-NHS (ammine dye NT-647-NHS) according to manufacturer indications (NanoTemper Technologies). Before MST experiments ...
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bioRxiv - Molecular Biology 2023Quote: The melting temperature Tm of a TadA protein was determined using nano differential scanning fluorimetry (nanoDSF,Nanotemper Prometheus NT.48 system) [40] ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Purified TRIM21 PRYSPRY domain was labeled using Protein Labeling Kit RED-NHS 2nd Generation (MO-L011, NanoTemper Technologies GmbH, Munich, Germany). Labeled protein was diluted to 50 nM in PBST (137 mM NaCl ...
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bioRxiv - Cancer Biology 2023Quote: ... Thermal unfolding was followed by differential scanning fluorimetry (DSF) by measuring intrinsic protein fluorescence using a Prometheus NT.48 (NanoTemper Technologies). Aliquots (10 μl ...
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bioRxiv - Biochemistry 2024Quote: The binding affinity of each recombinant TrK13 protein for heme was evaluated by MST analysis using the Monolith NT.115 instrument (NanoTemper Technologies). The recombinant TrK13 proteins (50 nM ...
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bioRxiv - Plant Biology 2024Quote: ... Purified insect cell-expressed RACK1A was fluorescently labeled with Protein Labeling Kit RED-NHS 2nd Generation (NanoTemper Technologies GmbH MO-L011) via amine conjugation ...
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bioRxiv - Biophysics 2023Quote: ... and the intrinsic protein fluorescence was recorded at 330 and 350 nm using the Prometheus NT.Plex nanoDSF device (NanoTemper, Munich, Germany).
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bioRxiv - Cancer Biology 2024Quote: ... 90 µl of 200 nM protein mixed 1:1 with 100 nM RED-tris-NTA 2nd generation dye (MO-L018, Nanotemper Technologies) and incubated at room temperature for 30 minutes ...
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bioRxiv - Biochemistry 2020Quote: 50 µg synthesized sP26 (Davids Biotechnologie, Regensburg, Germany) was fluorescently labeled with the Monolith NT Protein Labeling Kit RED (NanoTemper, Munich, Germany) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2019Quote: BRCA1 tBRCT wild type or mutant domains used in the assay were labeled with NT-647-NHS fluorescent dye using the Monolith NT™ Protein Labeling Kit (NanoTemper Technologies). Assays were carried out in 20mM Tris buffer ...
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bioRxiv - Biochemistry 2021Quote: Fluorescence labeling of the target protein (MDH) was performed by Red N-hydroxy succinimide (NHS) 2nd generation (NanoTemper Technologies GmbH, München, Germany) following the manufacturer’s protocol ...
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bioRxiv - Biophysics 2021Quote: The thermal unfolding experiments of the IgLC samples as a function of protein and denaturant concentration were performed with a Prometheus Panta instrument (Nanotemper, Munich, Germany). This is a microcapillary-based (10 μl sample per capillary ...
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bioRxiv - Plant Biology 2022Quote: The purified protein in PBS at a concentration of ∼16 µM was loaded into capillaries (nanoDSF grade standard, NanoTemper GmbH, Munich, Germany) and a melting curve was determined by using the nanoDSF Prometheus NT.48 instrument (NanoTemper GmbH ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 µM His6-SUMO-AGO1-NcGN was orthogonally labeled on Cystein-192 using the Monolith NT™ Protein Labeling Kit Red – Maleimide (NanoTemper Technologies). The labeling was performed according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Each protein dilution was mixed with one volume of labeled DNA and filled into Monolith NT standard treated capillaries (NanoTemper Technologies GmbH). Thermophoresis was measured using a Monolith NT.115 instrument (NanoTemper Technologies GmbH ...
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bioRxiv - Cell Biology 2024Quote: MST measurements were carried out using premium-coated capillaries to reduce non-specific interaction of the proteins with the glass surface on a Monolith NT.115 red-blue (Nanotemper, Munich, Germany), as described previously (Zuidema et al. ...
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bioRxiv - Biophysics 2020Quote: ... PD-1 and its mutant were fluorescently labelled according the standard labeling protocol of the NanoTemper Protein Labeling Kit RED-NHS (L001-NanoTemper Technologies, Munich, Germany). In MST experiments ...
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bioRxiv - Microbiology 2019Quote: The binding affinities of anti-CRISPR proteins to SpyCas9 were calculated using microscale thermophoresis (MST) on the Monolith NT.115 instrument (NanoTemper Technologies GmbH, Munich, Germany). For AcrIIA1/AcrIIA2b.3 with WT or mutant Cas9-gRNA complexes ...
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bioRxiv - Plant Biology 2022Quote: ... 50 nM of protein sample in PBS together with indicated concentrations of nucleotides were loaded into standard Monolith NT.115 capillaries (NanoTemper Technologies GmbH, Munich, Germany). MST measurement was performed using the Monolith NT.115 instrument (NanoTemper Technologies GmbH ...
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bioRxiv - Biophysics 2024Quote: The (I91)2-ClfAN2N3-(I91)2 polyprotein was fluorescently labeled by utilizing the commercial protein Labeling Kit RED-NHS 2nd Generation (Nanotemper Technologies GmbH, Munich, Germany). The dye carries a reactive NHS-ester group that reacts with primary amines in the protein to form a covalent bond ...
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bioRxiv - Biochemistry 2021Quote: Fluorophore-labeled protein was incubated with various ligands at room temperature for 15 minutes before measuring on Monolith NT.115 (NanoTemper, 80% LED power, 40% MST power). For competition assays ...