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Citations for NanoTemper :
151 - 200 of 268 citations for Insulin like growth factor binding protein 6 IGFBP 6 Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... CRBN protein was labelled with RED-Maleimide 2nd Generation dye (NanoTemper) optimized for degree-of-labeling of 1:1 molar ratio calculated by nanodrop ...
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bioRxiv - Biophysics 2024Quote: ... Protein Labeling Kit RED-NHS 2nd Generation was purchased from NanoTemper Technologies (München ...
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bioRxiv - Biophysics 2021Quote: ... or Monolith NT™ (Protein Labeling Kit Blue-NHS, NanoTemper GmbH, Germany). The labeling was achieved by incubating 115 μM of protein and 350 μM of dye (465 μl reaction volume ...
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bioRxiv - Microbiology 2023Quote: Protein thermostability was evaluated with a Prometheus NT.48 instrument (NanoTemper Technologies). Proteins at a concentration of 1 mg/mL were loaded to the grade capillaries and the intrinsic fluorescence signal was measured while temperature was increased by 1°C/min ...
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bioRxiv - Biochemistry 2023Quote: ... the proteins were labeled with a RED-NHS Labeling Kit (NanoTemper Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Ten microliters of proteins were loaded into a capillary (PR-C002, Nanotemper) and thermal denaturation was recorded using a 1 °C/min ramp from 20 to 95 °C ...
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bioRxiv - Biochemistry 2023Quote: ... Monolith protein labeling kit RED-NHS 2nd generation was purchased from NanoTemper Technologies (Munich ...
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bioRxiv - Biochemistry 2024Quote: ... the proteins were labeled with a RED-NHS Labeling Kit (NanoTemper Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: Protein thermal stability was measured using nanoDSF on a Prometheus panta instrument (NanoTemper) (39) ...
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bioRxiv - Biochemistry 2020Quote: ... 10μL of the diluted protein was loaded into NanoDSF Grade Standard Capillaries (NanoTemper) and melting temperatures were determined using a Prometheus NT4.8 NanoDSF instrument according to the manufacturer’s instruction ...
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bioRxiv - Biochemistry 2022Quote: ... 150 mM NaCl using the Monolith NT Protein Labelling Kit RED (NanoTemper Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: We determined protein melting temperatures using the Prometheus NT.48 instrument (NanoTemper Technologies). Purified FabI was diluted to a concentration of 25 μM in 50 nM HEPES ...
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bioRxiv - Microbiology 2021Quote: NfrB414-745 was labeled using the Protein Labeling Kit RED-NHS (NanoTemper Technologies). The labeling reaction was performed according to the manufacturer’s instructions in the supplied labeling buffer applying a concentration of 20 μM protein at room temperature for 30 min in the dark ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein series was loaded into Monolith NT.115 Premium Capillaries (NanoTemper Technologies) and thermophoresis was measured using MO.Control v1.6.1 software ...
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bioRxiv - Cell Biology 2023Quote: IRE1αLD was labelled using the Monolith NT protein labelling Kit Red-Maleimide (NanoTemper). The concentration of the labelled IRE1αLD was kept constant at 10 nM ...
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bioRxiv - Biochemistry 2023Quote: Protein Labeling Kit RED-maleimide 2nd Generation (cat# MO-L014; NanoTemper Technologies GmbH) was used for covalent labeling of LC3A cystine residues ...
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bioRxiv - Biophysics 2022Quote: Recombinantly purified protein constructs were labelled with RED-tris NTA dye (NanoTemper Technologies GmbH) in PBS to a concentration of 100 nM ...
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bioRxiv - Cell Biology 2021Quote: ... Purified tubulin was labeled using the Monolith protein labeling kit RED-NHS (NanoTemper Technologies) and then diluted to 200 nM concentration before incubated with 2 μM eribulin for 20min at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: SAE1/SAE2 was labelled using Protein Labelling Kit RED-NHS 2nd Generation (NanoTemper Technologies). The labelling reaction was performed according to the manufacturer’s instructions in the supplied labelling buffer ...
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bioRxiv - Biophysics 2022Quote: JIP3-Nter fragments were labelled using the Protein Labelling Kit Blue-NHS (NanoTemper Technologies). The labelling reaction was performed according to the manufacturer’s instructions in the supplied labelling buffer applying a concentration of 20 μM protein (molar dye:protein ratio ≈ 3:1 ...
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bioRxiv - Plant Biology 2023Quote: ... mixed 1:1 with the labelled protein and loaded into standard capillaries (NanoTemper Technologies). MST measurements were recorded using 20 % LED power and 20 to 80 % MST power and analysed using MO ...
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bioRxiv - Biophysics 2024Quote: ... The melting temperature for characterization of protein thermal stability were done using NanoDSF (NanoTemper) with protein concentration at 0.2mg/ml ...
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bioRxiv - Molecular Biology 2021Quote: ... Nanobodies were labeled using the lysine reactive Monolith NT Protein Labeling kit RED-NHS (NanoTemper). As TCNB11 has a lysine residue in CDR2 ...
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bioRxiv - Biophysics 2022Quote: ... was labeled with RED-NHS (2nd Generation) dye using the Monolith Protein Labeling Kit (NanoTemper Technologies ...
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bioRxiv - Biochemistry 2021Quote: ... g5Rp was first labeled using the Monolith NT Protein Labeling Kit RED-NHS (NanoTemper Technologies), and the labeled protein was then diluted to 20 nM with buffer containing 50 mM HEPES ...
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bioRxiv - Neuroscience 2023Quote: Thermal denaturation of ApoE proteins was studied by using a NanoDSF Prometheus instrument (NanoTemper, Germany). The protein samples at a concentration of 0.2 mg/ml were heated at a scan rate of 1 °C/min from 25 to 95 °C ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We used the Monolith Protein Labeling Kit RED-NHS 2nd Generation kit (NanoTemper MO- L011) to label ∼1 lysine per Fcy1 monomer with a fluorophore following the manufacturer’s instruction ...
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bioRxiv - Evolutionary Biology 2024Quote: We measured protein stability using nano-differential scanning fluorimetry on a Prometheus NT.48 (NanoTemper). After purification and his-tag cleavage ...
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bioRxiv - Biochemistry 2024Quote: ... FabF protein was labeled with the RED-MALEIMIDE or RDE-NHS dye (Nanotemper Technologies, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2019Quote: The stability of the different proteins was measured by nanoDSF (Prometheus NT.48, NanoTemper Technologies, GmbH). The proteins were first dialyzed against 1 l of gel filtration buffer supplemented with 5 mM EDTA overnight at 4 °C and subsequently 2x against 1 l of gel filtration buffer without EDTA overnight at 4 °C ...
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bioRxiv - Cancer Biology 2019Quote: KDM4B was labeled with a cysteine reactive fluorescent dye (Protein Labeling Kit RED-MALEIMIDE, Nanotemper GmbH). A serial dilution of titrant was prepared in MST buffer containing 10 mM HEPES (pH 7.5) ...
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bioRxiv - Microbiology 2020Quote: ... Each protein (10 μM) was labeled with NT-647-NHS reactive dye (30 μM) (Nanotemper, Germany), which reacted efficiently with the primary amines of the proteins to form a stable dye protein conjugate ...
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bioRxiv - Molecular Biology 2022Quote: The thermal stability of the proteins was determined using Prometheus NT.48 nanoDSF instrument from NanoTemper Technologies ...
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bioRxiv - Molecular Biology 2022Quote: ... 10 μM PfCoSP was labeled using NanoTemper’s Protein Labeling Kit RED-NHS (L001, NanoTemper technologies, Germany). The concentration of labeled PfCoSP protein was kept constant at a concentration of 90 nM ...
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bioRxiv - Cell Biology 2020Quote: ... His6-MyoVa GTD was labeled using RED-NHS 2nd Generation (Amine Reactive) Protein Labeling Kit (Nanotemper Technologies). For all experiments ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by labelling with 30uM Cysteine reactive dye (Monolith Protein Labelling Kit Red-Maleimide 2nd Generation, NanoTemper), and incubated in dark at RT for 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... The RNA-protein mixtures were analyzed in premium capillaries using the Monolith NT.115T MST (NanoTemper Technologies). Measurements were carried out at 25 °C ...
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bioRxiv - Biochemistry 2022Quote: ... K17A and K18A were labeled using RED-NHS 2nd Generation (Amine Reactive) Protein Labeling Kit (Nanotemper Technologies). For all experiments ...
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bioRxiv - Biochemistry 2022Quote: NanoDSF and light scattering of purified proteins were measured with a Prometheus NT.48 (Nanotemper, Munich, Germany), equipped with back-reflection optics or with a Prometheus Panta (Nanotemper ...
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bioRxiv - Biochemistry 2022Quote: ... Then the proteins were loaded onto Prometheus NT.48 nanoDSF Grade Standard Capillaries (PR-C002, NanoTemper Technologies), the fluorescence signal was recorded under a temperature gradient ranging from 25 to 90°C (incremental steps of 0.5°C min-1) ...
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bioRxiv - Biochemistry 2022Quote: ... The full-length SLC19A1 protein was fluorescently labelled with a Red-NHS 2nd Generation kit (NanoTemper Technologies) in Buffer D (25 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: Protein thermal stability was determined by differential scanning fluorimetry (nanoDSF) on Prometheus NT.48 instrument (Nanotemper Technologies) in nanoDSF grade standard capillaries filled with the samples ...
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bioRxiv - Cell Biology 2023Quote: ... NRP-1 b1b2 domains were labeled using the Protein Labeling Kit RED-NHS (NanoTemper Technologies, Munich, Germany). The interaction of NRP-1 structural variants with FOL-peptides was measured using a Monolith NT.115 [NT.115Pico/NT.LabelFree] instrument (NanoTemper Technologies ...
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bioRxiv - Microbiology 2024Quote: ... The Csy complexes were fluorescence-labeled using the Protein Labeling Kit RED-NHS 2nd Generation (NanoTemper Technologies) at 10 μM ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Protein and compound mixtures were loaded into standard treated capillaries (MO-K022, NanoTemper Technologies GmbH, Munich, Germany). Measurements were performed on a Monolith NT.115 with blue/red filters (NanoTemper Technologies GmbH ...
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bioRxiv - Biochemistry 2024Quote: Thermal stability of proteins was analyzed using nanoscale differential scanning fluorimetry with a Prometheus NT.48 (NanoTemper) device ...
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Undruggable oncoproteins cMyc and NMyc bind to mediator of transcription with superior high affinitybioRxiv - Biochemistry 2024Quote: ... The quality of the protein was determined using Differential Scanning Fluorimetry (Prometheus NT.48, NanoTemper Technologies GmbH). The uniform labelling of KIX domain was confirmed by MALDI-TOF MS.
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bioRxiv - Biochemistry 2021Quote: Protein thermal stability was measured in a label-free fluorescence assay using a Prometheus NT.48 (NanoTemper, Germany). Briefly ...
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bioRxiv - Biochemistry 2020Quote: His6-GlnK1 was purified and labeled using Monolith Protein Labeling Kit RED-NHS 2nd Generation (NanoTemper, Munich, Germany) (DOL ...
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bioRxiv - Biochemistry 2020Quote: ... Purified DVD-actin was labelled using Monolith NT protein labelling kit RED-NHS (Amine Reactive) dye (NanoTemper Technologies) according to the manufacturer’s instructions ...