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Citations for NanoTemper :
1 - 50 of 202 citations for Human Chemokine Like Factor Superfamily 6 CKLFSF6 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Protein quality was verified using a Tycho NT.6 system (NanoTemper) and ACE2 activity assay kit (SensoLyte ...
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A pectin-binding peptide with a structural and signaling role in the assembly of the plant cell wallbioRxiv - Plant Biology 2023Quote: ... protein quality was assessed using a Tycho NT.6 (NanoTemper, Munich, Germany). Labeled protein was dissolved in MST buffer (50mM Tris-HCl pH 7.8 ...
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bioRxiv - Pathology 2021Quote: ... Protein quality was determined using a Tycho NT.6 (Figures S4 & S5) (NanoTemper Technologies). Human hnRNPC was purchased from Abnova (catalog# H00003183P01S.)
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bioRxiv - Biochemistry 2022Quote: ... Protein folding and quality control was accomplished using nDSF on a Tycho NT.6 (NanoTemper) which monitors protein thermal unfolding using intrinsic fluorescence at 330 and 350 nm ...
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bioRxiv - Biochemistry 2019Quote: The thermal stability of wild-type and variant RmtC proteins was assessed using a Tycho NT.6 instrument (NanoTemper) to ensure protein quality between different preparations of proteins and before/ after freezing ...
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bioRxiv - Plant Biology 2021Quote: ... 6 (NanoTemper Technologies). Assays were done at 5.8 μM protein in 0.2 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biophysics 2020Quote: ... 6 (NanoTemper Technologies). Spike variants were diluted (0.15 mg/ml ...
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bioRxiv - Biochemistry 2020Quote: ... 6 (NanoTemper Technologies). Spike variants were diluted (0.15 mg/ml ...
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bioRxiv - Biochemistry 2020Quote: ... 6 (NanoTemper Technologies). Pre-dialyzed protein and mutant samples were diluted (∼0.5 mg/mL ...
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bioRxiv - Molecular Biology 2023Quote: ... 6 (NanoTemper Technologies). Spike ectodomains were diluted to approximately 0.15LJmg/mL ...
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bioRxiv - Biochemistry 2022Quote: Protein melting profiles using 5 μM of 4B7- or 1245-Db were measured with Tycho NT.6 (NanoTemper® Technologies GmbH). Fluorescence emission intensities was measured at I350 and I330 in triplicates and its ratio plotted as a function of temperature ...
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The activator domain of bacterial collagenases drives collagen recognition, unwinding and processingbioRxiv - Biochemistry 2023Quote: ... 6 (NanoTemper Technologies, Germany). The measurements were performed at 0.1 mg/ml protein concentration in 15 mM Tris-SO4 pH 7.5 ...
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bioRxiv - Microbiology 2024Quote: ... using the Tycho NT.6 (NanoTemper). DSF experiments were set up to a final volume of 10 µl of buffer C containing the EccC5DUF construct at 2 µM in the absence or in the presence of ADP-AlF3 at 4 mM ...
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bioRxiv - Neuroscience 2021Quote: ... Recombinant human FTO protein was labeled with His-tag using Monolith His-Tag Labeling Kit RED-tris-NTA (NanoTemper Technologies GmbH; MO-L008). The labelled FTO protein (target ...
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bioRxiv - Biochemistry 2020Quote: ... using a Tycho NT.6 instrument (Nanotemper), which allowed for determination of NT-mini’s unfolding temperatures in the different assayed conditions ...
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bioRxiv - Biochemistry 2023Quote: We used the Tycho NT.6 (NanoTemper Technologies) for label-free thermal shift analysis ...
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bioRxiv - Biochemistry 2021Quote: ... and RBD-71 measured using the Tycho NT.6 (NanoTemper).
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bioRxiv - Biochemistry 2024Quote: DSF assays were performed using Tycho NT.6 (NanoTemper Technologies). RBDs were diluted to approximatively 1 mg/mL ...
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bioRxiv - Biochemistry 2020Quote: His6-GlnA1 was analyzed using Tycho NT.6 (NanoTemper, Munich, Germany) by applying a standard capillary (10 µl ...
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bioRxiv - Biophysics 2023Quote: Thermal shift assay was performed using Tycho NT.6 (NanoTemper Technologies). Envelope ectodomain SOSIPs were diluted (0.15 mg ml−1 ...
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bioRxiv - Biochemistry 2022Quote: ... Data were acquired with a Tycho NT.6 equipment (NanoTemper Technologies). The emission fluorescence intensity was recorded at 330 nm and 35 ºC ...
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bioRxiv - Immunology 2023Quote: Thermal shift assay was performed using Tycho NT.6 (NanoTemper Technologies). Envelope ectodomain SOSIPs were diluted (0.15LmgLml−1 ...
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bioRxiv - Biochemistry 2024Quote: The thermal stability was performed using Tycho NT.6 (NanoTemper Technologies). SIDT1ECD and kifunensine-treated SIDT1ECD were diluted to a final concentration of 0.25 mg/mL at various pH (pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: Proteins were labeled using the Monolith NT Protein Labeling Kit RED (NanoTemper Technologies) according to the manufacturer’s protocol and using a buffer consisting of 20 mM HEPES ...
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bioRxiv - Molecular Biology 2022Quote: ... We used Tycho analysis (Tycho NT.6, NanoTemper Technologies GmbH, Munich, Germany) to follow the interaction between VcDnaB or EcDnaB and VcDciA ...
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bioRxiv - Immunology 2019Quote: ... ILT7 purified protein was labeled using the Protein Labeling Kit RED-NHS (NanoTemper Technologies). The labeling reaction was performed according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... EMCV 2A protein was labelled using the Protein Labelling Kit RED-Maleimide (NanoTemper Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: Protein-protein interactions were monitored by MST using a Monolith NT.115 instrument (Nanotemper Technologies). His6-MyoVa GTD was labeled using RED-NHS 2nd Generation (Amine Reactive ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were labeled using the RED-tris-NTA His tag protein labeling kit (Nanotemper Technologies). Labelled protein was added to a serial dilution of unlabeled DAG ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were labeled using the RED-tris-NTA His tag protein-labeling kit (Nanotemper Technologies). Labeled proteins were added to a serial dilution of unlabeled DAG or liposomes prepared in binding buffer (20 mM Tris ...
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bioRxiv - Cancer Biology 2023Quote: ... EED and JMJD3 proteins were labeled with Protein Labeling kit RED-NHS (NanoTemper, MO-L001). At least three independent experiments were performed for each binding reaction.
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bioRxiv - Biochemistry 2023Quote: The thermal stability analysis was conducted using the Tycho NT.6 (NanoTemper Technologies). SIDT1ECD and SIDT2ECD were diluted to a final concentration of 0.25 mg/mL at different pH values (pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... the samples were loaded into capillary tubes for Tycho NT.6 instrument (NanoTemper). Tryptophan fluorescence at emission wavelengths of 350 nm and 330 nm were measured as the sample temperature was ramped from 35 °C to 95 °C according to the preprogrammed instrument protocol ...
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bioRxiv - Biochemistry 2019Quote: Solution protein-protein binding was monitored by microscale thermophoresis (MST) with a Monolith NT.115 (Nanotemper). The target proteins were portions of the CsoS2 NTD fused to Superfolder GFP and used at a concentration of 50 nM ...
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bioRxiv - Biochemistry 2022Quote: Protein–protein interactions were monitored by microscale thermophoresis using a Monolith NT.115 instrument (Nanotemper Technologies). His LRRK2 Armadillo (1-552) ...
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bioRxiv - Biochemistry 2023Quote: Protein–protein interactions were monitored by microscale thermophoresis using a Monolith NT.115 instrument (NanoTemper Technologies). His LRRK2 Armadillo (1–552 ...
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bioRxiv - Biophysics 2023Quote: ... where the protein was labelled with Monolith Protein labelling kit RED-NHS (NanoTemper Technologies, Munich, Germany). In the binding assay ...
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bioRxiv - Biophysics 2023Quote: ... and experiments were carried out using the NanoTemper Tycho NT.6 (NanoTemper Technologies, Germany). The temperature gradient was set to an increase of 30 °C/min from 35° C to 95° C ...
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bioRxiv - Microbiology 2022Quote: ... Protein-protein interactions were analyzed at 20°C by MST on a Monolith NT.115 (NanoTemper technologies) using standard capillaries ...
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bioRxiv - Physiology 2023Quote: ... Purified OsABG protein was labeled using the protein labeling kit Red-NHS 2nd generation (MO-L011, Nanotemper) which dyes primary lysine residues in the protein ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were fluorescently labeled using the RED-Maleimide 2nd Generation Protein Labeling Kit (NanoTemper Technologies GmbH, Germany) as recommended by the manufacturer ...
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bioRxiv - Microbiology 2019Quote: Thermal unfolding experiments of AtaC were performed with a Tycho NT.6 instrument (NanoTemper Technologies). The samples were heated in a glass capillary at a rate of 30 K/min and the internal fluorescence at 330 nm and 350 nm was recorded ...
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bioRxiv - Molecular Biology 2023Quote: The affinity between RNA and protein and between proteins was measured by MST on a Monolith NT.115 (NanoTemper, Germany). Replicates represent individual measurements performed on different days using proteins from the same prep ...
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bioRxiv - Cancer Biology 2023Quote: Label-free thermal shift assay experiments were performed with a Tycho NT.6 instrument (NanoTemper Technologies) based on a reported protocol 76 ...
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bioRxiv - Biochemistry 2020Quote: ... His-tagged protein (Pry1 or Na-ASP-2) was fluorescently labeled using the RED-tris-NTA His tag protein labeling kit (Nanotemper Technologies). Labeled protein (Pry1 or Na-ASP-2 ...
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bioRxiv - Biochemistry 2023Quote: ... Purified GST fusion protein were fluorescently labeled using the Monolith series Protein Labeling Kit RED-NHS 2nd Generation (Nanotemper, MO-L011) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: Thermal stability was evaluated by nanoDSF using the Tycho NT.6 system (NanoTemper Technologies GmbH, München, Germany) 68 ...
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bioRxiv - Biophysics 2020Quote: Samples thermostability was measured by nano differential scanning fluorimetry using a Tycho NT.6 instrument (NanoTemper Technologies) that measures the intrinsic fluorescence from tryptophan and tyrosine residues detected at emission wavelengths of 350 and 330 nm ...
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bioRxiv - Plant Biology 2021Quote: ... 0.5 mM NaCl and run in duplicates in Tycho NT.6 capillaries (NanoTemper Technologies, Cat# TY-C001). Different protein solutions were prepared to reach a final CaCl2 concentrations of 0.01 mM ...
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bioRxiv - Microbiology 2021Quote: ... Phr2 and Pga4 proteins and Bull Serum Albumin (BSA) were labeled using the Monolith™ RED-NHS Protein Labeling Kit (NanoTemper Technologies) according to the manufacturer’s instructions ...