Labshake search
Citations for NanoTemper :
1 - 50 of 143 citations for Estrone 3 Glucuronide E1G ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Affinity Analysis 3 Software (Nanotemper) using a Kd fit model and constraining the target concentration.
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bioRxiv - Microbiology 2022Quote: Purified WT or mutant NSP12 recombinant proteins were labeled with Cytidine-5 dye using the Monolith His-Tag labeling kit (NanoTemper Technologies), and then labeled NSP12 proteins were diluted to 700 nM in PBST buffer ...
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bioRxiv - Biophysics 2021Quote: The thermal and chemical denaturation of the WT and the mutant 14-3-3 proteins were performed using nano-DSF (Prometheus NT.48) from Nanotemper Technologies (Munchen ...
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bioRxiv - Molecular Biology 2021Quote: ... 20 µL reactions were incubated at room temperature for 5 min and 5 µL of the mixture was loaded into NT.115 capillaries (NanoTemper). MST was performed in Monolith NT.115 (NanoTemper ...
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bioRxiv - Biochemistry 2023Quote: ... centrifuged for 5 min and loaded to standard capillaries (Nanotemper, #MO-K022). To investigate competitive effects of different ligands ...
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bioRxiv - Microbiology 2020Quote: ... Microscale thermophoresis was completed in 3 independent repeat experiments on a Monolith NT.115 instrument (NanoTemper Technologies), running MO.Control software with settings of 60-80% excitation power and 40% MST power at room temperature.
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bioRxiv - Cell Biology 2022Quote: ... employing the Monolith His-Tag Labeling Kit (RED-tris-NTA 2nd Generation kit, NanoTemper). The labeled protein was dissolved in a buffer containing PBS ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We used the Monolith Protein Labeling Kit RED-NHS 2nd Generation kit (NanoTemper MO- L011) to label ∼1 lysine per Fcy1 monomer with a fluorophore following the manufacturer’s instruction ...
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bioRxiv - Biochemistry 2019Quote: ... was monitored during unfolding over a linear temperature gradient (35-95 °C) over 3 minutes on a Tycho NT.6 instrument (NanoTemper). The temperature at which a transition occurs in a plot of the first derivative of the fluorescence ratio (350/330 nm) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and then loaded into 3 standard capillaries for triplicate readings in a Prometheus NT.48 instrument (Nanotemper Technologies, Munich, Germany). Experiments were run using 40% excitation power ...
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bioRxiv - Biophysics 2019Quote: ... Nucleoprotein samples in PBS buffer solution (at concentrations of 3 mg/ml) were loaded into quartz capillaries (Standard Capillaries Prometheus NT.48, NanoTemper); capillaries were placed on the platform and measurements were taken in the temperature range from 15°C to 95°C in increments of 1°C /min at a laser intensity of 50% ...
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bioRxiv - Biophysics 2022Quote: ... HBc/4M2e samples in PBS buffer solution (at concentrations of 3 mg/ml) were collected in glass capillaries (Standard Capillaries Prometheus NT.48, NanoTemper), capillaries were placed on the platform and measurements were taken in the temperature range from 15°C to 95°C in increments of 1°C /min at a laser intensity of 50% ...
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bioRxiv - Biochemistry 2019Quote: ... was labeled with Monolith His-Tag Labeling Kit RED-tris-NTA kit (NanoTemper Technologies, München, Germany). Briefly ...
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bioRxiv - Biochemistry 2022Quote: ... 13000 rpm), filled into Prometheus high-sensitivity capillaries (10 μL each, n=3) and transferred to a Prometheus Panta instrument (NanoTemper Technologies). DLS analysis of BbHtrA S/A rh and PDI were determined using Prometheus Panta’s size analysis function.
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bioRxiv - Molecular Biology 2020Quote: ... We measured the fluorescence signal from Gαi1β1γ1 by using the Monolith His-Tag Labeling Kit RED-tris-NTA 2nd Generation kit (NanoTemper Technologies). The samples were prepared in a buffer containing 20 mM sodium phosphate (pH 6.9) ...
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bioRxiv - Microbiology 2023Quote: ... + 0.5% DDM and was labeled using a His-Tag Labeling Kit RED-tris-NTA 2nd Generation Kit (NanoTemper) via the manufacturers protocol in 1.7 ml low-adhesion tubes ...
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bioRxiv - Biophysics 2021Quote: 6xHis-RAD51 was labelled with the Monolith His-Tag Labeling Kit RED-tris-NTA 2nd Generation kit (NanoTemper Technologies), which specifically recognizes the hexahistidine tail of the recombinant protein.
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bioRxiv - Molecular Biology 2021Quote: ... between protein:compound sample and a protein only sample at 1.5-2-5 sec is calculated and plotted through MO.Affinity analysis v2.3 (NanoTemper Technologies) and GraphPad Prism 8.0.0 (GraphPad Software ...
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bioRxiv - Neuroscience 2023Quote: ... Control experiments included measurements of the binding of the RED-tris-NTA dye to His-DJ-1 and of a dye-labeled AptamerCy5 to AMP using a manufacturer kit (Monolith NT.115 Control Kit RED, NanoTemper).
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bioRxiv - Microbiology 2024Quote: ... and the NHS-red-tag kit (Nanotemper, Germany) was used to label ConA (Sigma ...
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bioRxiv - Biochemistry 2022Quote: ... Measurements were done in triplicate at multiple concentrations (50 µM, 5 µM, and 0.5 µM) in Tycho standard capillaries (Nanotemper Technologies). Data analysis was performed via MO Affinity Analysis Software v2.1.3 (Nanotemper Technologies ...
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bioRxiv - Microbiology 2023Quote: ... around 5 μl of the samples were loaded in MonolithTM Capillaries and MST analysis was carried out on the Instrument (Nanotemper).
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bioRxiv - Biochemistry 2023Quote: The BD5.1 protein was labelled with the Monolith His-Tag Labeling Kit (RED-tris-NTA 2nd Generation kit, NanoTemper Technologies, Catalog# MO-L018) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... Reaction mixtures were incubated for 15 min at 25°C and approximately 5 µL of solution was loaded into Monolith NT Standard Capillaries (NanoTemper Technologies). Measurements were performed at 25°C using 20% LED and 20% IR-laser power with 5 s/30 s/5 s laser off/on/off times ...
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bioRxiv - Biochemistry 2022Quote: Protein melting profiles using 5 μM of 4B7- or 1245-Db were measured with Tycho NT.6 (NanoTemper® Technologies GmbH). Fluorescence emission intensities was measured at I350 and I330 in triplicates and its ratio plotted as a function of temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... selected linear/circular aptamers were equally diluted with 5’ Cy5 labeled complementary strand to reach the optimum concentrations of 5 nM where satisfying fluorescence intensity was obtained for KD determination using Monolith device (NanoTemper, Germany). Affinity of the selected aptamers was evaluated in 16 test tubes where spike/RBD proteins were diluted by double from the initial concentration 1 mM and then 5 nM of aptamer/Cy5 labeled complementary strand complex were added to each protein dilution in final volume of 20 μl ...
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bioRxiv - Microbiology 2020Quote: ... Monolith His-Tag Labeling kit (MO-L018) was from NanoTemper Technologies.
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bioRxiv - Cell Biology 2023Quote: ... Monolith RED-NHS 2nd generation protein labeling kit (NanoTemper Technologies GmbH) was used to label LIAS and ...
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bioRxiv - Biophysics 2024Quote: ... Protein Labeling Kit RED-NHS 2nd Generation was purchased from NanoTemper Technologies (München ...
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bioRxiv - Biochemistry 2023Quote: ... and centrifuged at 20k ×g at 4 °C for 5 min prior to loading into standard-coated capillary tubes (NanoTemper, GmbH, Munich, Germany). Microscale thermophoresis data were collected on a Monolith NT.115 (NanoTemper ...
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bioRxiv - Biophysics 2021Quote: ... or Monolith NT™ (Protein Labeling Kit Blue-NHS, NanoTemper GmbH, Germany). The labeling was achieved by incubating 115 μM of protein and 350 μM of dye (465 μl reaction volume ...
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bioRxiv - Cell Biology 2023Quote: His-Tag labeling Kit RED-tris-NTA 2nd generation (MO-L018, Nanotemper) was used to label His-tagged Bax purchased from Creative BioMart (Catalog No ...
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bioRxiv - Biochemistry 2023Quote: ... using a His-tag Labeling Kit (NanoTemper Technologies, cat. no. MO-L018). For measurements using CBP30 ...
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bioRxiv - Biochemistry 2023Quote: ... the proteins were labeled with a RED-NHS Labeling Kit (NanoTemper Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Monolith protein labeling kit RED-NHS 2nd generation was purchased from NanoTemper Technologies (Munich ...
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bioRxiv - Biochemistry 2024Quote: ... the proteins were labeled with a RED-NHS Labeling Kit (NanoTemper Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... MnCl2 and DTT were added just before the experiment) and incubated at RT for 5 min before samples were loaded with premium capillaries (NanoTemper Technologies, cat. no. MO-K025). Data were recorded sequentially at 40% ...
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bioRxiv - Microbiology 2021Quote: Proteins were labeled using the Monolith NT Protein Labeling Kit RED (NanoTemper Technologies) according to the manufacturer’s protocol and using a buffer consisting of 20 mM HEPES ...
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bioRxiv - Biochemistry 2022Quote: ... 150 mM NaCl using the Monolith NT Protein Labelling Kit RED (NanoTemper Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: NfrB414-745 was labeled using the Protein Labeling Kit RED-NHS (NanoTemper Technologies). The labeling reaction was performed according to the manufacturer’s instructions in the supplied labeling buffer applying a concentration of 20 μM protein at room temperature for 30 min in the dark ...
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bioRxiv - Cell Biology 2023Quote: IRE1αLD was labelled using the Monolith NT protein labelling Kit Red-Maleimide (NanoTemper). The concentration of the labelled IRE1αLD was kept constant at 10 nM ...
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bioRxiv - Biochemistry 2023Quote: Protein Labeling Kit RED-maleimide 2nd Generation (cat# MO-L014; NanoTemper Technologies GmbH) was used for covalent labeling of LC3A cystine residues ...
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bioRxiv - Molecular Biology 2021Quote: TCNB4 was covalently labeled with Red-NHS using the Monolith NT kit (Nanotemper technologies) and used in the cellular binding and uptake studies ...
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bioRxiv - Cell Biology 2021Quote: ... Purified tubulin was labeled using the Monolith protein labeling kit RED-NHS (NanoTemper Technologies) and then diluted to 200 nM concentration before incubated with 2 μM eribulin for 20min at room temperature ...
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bioRxiv - Immunology 2019Quote: ... ILT7 purified protein was labeled using the Protein Labeling Kit RED-NHS (NanoTemper Technologies). The labeling reaction was performed according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... It was fluorescently labelled with the RED-NHS 2nd Generation kit (NanoTemper Technologies GmbH), and desalted to the reaction buffer (10 mM MES ...
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bioRxiv - Molecular Biology 2021Quote: ... EMCV 2A protein was labelled using the Protein Labelling Kit RED-Maleimide (NanoTemper Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: Purified SecB was randomly labelled using an NT-647-NHS labelling kit (Nanotemper Technologies). 160 nM NT-647-labelled SecB was incubated with the indicated concentrations of unlabelled AscA or AscAΔMBD in buffer (20 mM potassium HEPES [pH 7.5] ...
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bioRxiv - Molecular Biology 2022Quote: SAE1/SAE2 was labelled using Protein Labelling Kit RED-NHS 2nd Generation (NanoTemper Technologies). The labelling reaction was performed according to the manufacturer’s instructions in the supplied labelling buffer ...
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bioRxiv - Biophysics 2022Quote: JIP3-Nter fragments were labelled using the Protein Labelling Kit Blue-NHS (NanoTemper Technologies). The labelling reaction was performed according to the manufacturer’s instructions in the supplied labelling buffer applying a concentration of 20 μM protein (molar dye:protein ratio ≈ 3:1 ...